Enzymatic synthesis of a series of alkyl esters using novozyme 435 in a packed-bed, miniaturized, continuous flow reactor

2008 ◽  
Vol 26 (6) ◽  
pp. 466-472 ◽  
Author(s):  
L. L. Woodcock ◽  
C. Wiles ◽  
G. M. Greenway ◽  
P. Watts ◽  
A. Wells ◽  
...  
2012 ◽  
Vol 18 (21) ◽  
pp. 6604-6609 ◽  
Author(s):  
Lara Babich ◽  
Aloysius F. Hartog ◽  
Michael A. van der Horst ◽  
Ron Wever

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ewa Szczepańska ◽  
Danilo Colombo ◽  
Francesca Tentori ◽  
Teresa Olejniczak ◽  
Elisabetta Brenna ◽  
...  

AbstractThe demand for natural food flavorings increases every year. Biotransformation has become an attractive approach to obtain natural products. In this work, enantiomerically pure (R)-(+)-δ-decalactone was obtained by reduction of the C=C double bond of natural massoia lactone in a continuous-flow reactor. Of 13 different ene-reductases isolated, purified and tested, OYE3 was found to be the most efficient biocatalyst. The selected biocatalyst, either in the form of purified enzyme, cell lysate, whole cells or immobilized cells, was tested in the batch system as well as in the packed-bed flow bioreactor. The biotransformation performed in batch mode, using Ca2+-alginate immobilized cells of Escherichia coli BL21(DE3)/pET30a-OYE3, furnished the desired product with complete conversion in 30 min. The process was intensified using a continuous-flow reactor-membrane filtration system (flow 0.1 mL/min, substrate concentration 10 mM, pH 7, 24 °C) with cell lysate as biocatalyst combined with a cofactor regeneration system, which allowed obtaining > 99% bioconversion of massoia lactone.


2018 ◽  
Vol 69 (6) ◽  
pp. 1363-1366 ◽  
Author(s):  
Stefania Daniela Bran ◽  
Petre Chipurici ◽  
Mariana Bran ◽  
Alexandru Vlaicu

This paper has aimed at evaluating the concentration of bioethanol obtained using sunflower stem as natural support, molasses as carbon source and Saccharomyces cerevisiae yeast in a continuous flow reactor. The natural support was tested to investigate the immobilization/growth of S. cerevisiae yeast. The concentration of bioethanol produced by fermentation was analyzed by gas chromatography using two methods: aqueous solutions and extraction in organic phase. The CO2 flow obtained during the fermentation process was considered to estimate when the yeast was deactivated. The laboratory experiments have highlighted that the use of plant-based wastes to bioconversion in ethanol could be a non-pollutant and sustainable alternative.


2020 ◽  
Vol 8 (35) ◽  
pp. 13195-13205 ◽  
Author(s):  
Swathi Mukundan ◽  
Daria Boffito ◽  
Abhijit Shrotri ◽  
Luqman Atanda ◽  
Jorge Beltramini ◽  
...  

2019 ◽  
Vol 18 (2) ◽  
pp. 314-318 ◽  
Author(s):  
Martin Dilla ◽  
Ahmet E. Becerikli ◽  
Alina Jakubowski ◽  
Robert Schlögl ◽  
Simon Ristig

Newly developed tubular reactor geometry allows intensive gas–solid interaction in photocatalytic gas-phase CO2 reduction.


2017 ◽  
Vol 115 (3) ◽  
pp. 606-616 ◽  
Author(s):  
Stephanie A. Parker ◽  
Linus Amarikwa ◽  
Kevin Vehar ◽  
Raquel Orozco ◽  
Scott Godfrey ◽  
...  

2006 ◽  
Vol 691 (24-25) ◽  
pp. 5197-5203 ◽  
Author(s):  
Zenon Lysenko ◽  
Bob R. Maughon ◽  
Tezi Mokhtar-Zadeh ◽  
Michael L. Tulchinsky

Sign in / Sign up

Export Citation Format

Share Document