Facile synthesis of GalNAc monomers and block polycations for hepatocyte gene delivery

2021 ◽  
Author(s):  
Matthew R. Bockman ◽  
Rishad J. Dalal ◽  
Ramya Kumar ◽  
Theresa M. Reineke
Keyword(s):  
Gene Delivery ◽  
Synthetic Route ◽  
Facile Synthesis ◽  
Low Toxicity ◽  
Delivery Vehicles

Here, we present a facile synthetic route for a monomer displaying N-acetyl-d-galactosamine and subsequent copolymerization in a block format with cationic subunits readily accessing liver-targeted polymeric pDNA delivery vehicles with low toxicity.

scholarly journals Environmentally friendly and facile synthesis of Rh nanoparticles at room temperature by alkaline ethanol solution and their application for ethanol electrooxidation

RSC Advances ◽  
2017 ◽  
Vol 7 (6) ◽  
pp. 3161-3169 ◽  
Author(s):  
Fuhai Li ◽  
Hanqin Weng ◽  
Yun Shang ◽  
Zuoming Ding ◽  
Zheng Yang ◽  
...  
Keyword(s):  
Room Temperature ◽  
Environmentally Friendly ◽  
Ethanol Solution ◽  
Ethanol Electrooxidation ◽  
Synthetic Route ◽  
Facile Synthesis

A facile, fact and green synthetic route was developed to prepare Rh nanoparticles at room temperature.

Adeno-associated Viruses as Liver-Directed Gene Delivery Vehicles: Focus on Lipoprotein Metabolism

2013 ◽  
pp. 273-307 ◽  
Author(s):  
William R. Lagor ◽  
Julie C. Johnston ◽  
Martin Lock ◽  
Luk H. Vandenberghe ◽  
Daniel J. Rader
Keyword(s):  
Gene Delivery ◽  
Lipoprotein Metabolism ◽  
Delivery Vehicles ◽  
Gene Delivery Vehicles

A carbon-based nanocarrier for efficient gene delivery

2021 ◽  
Author(s):  
Tahereh Kashkoulinejad-Kouhi ◽  
Shadi Sawalha ◽  
Shahrokh Safarian ◽  
Blanca Arnaiz
Keyword(s):  
Hydrogen Peroxide ◽  
Gene Delivery ◽  
Water Solubility ◽  
Chemotherapy Resistance ◽  
A549 Cells ◽  
Carbon Nanodots ◽  
Simple Method ◽  
Efficient Gene ◽  
Low Toxicity ◽  
Positively Charged

Aim: Several types of nanocarriers, most of which show significant cytotoxicity, have been developed to overcome the problem of gene-delivery barriers. Biocompatibility, low toxicity and water solubility of carbon nanodots (CNDs) are major advantages that recommend them as delivery systems. Materials & methods: We present a simple method to produce positively charged CNDs. Ethanolamine, ethylenediamine and hydrogen peroxide were utilized to synthesize these CNDs. Results & conclusion: Our results indicated that delivery of the CND–siGFP complex led to significant switching-off of the fluorescence of the GFP-expressing A549 cell. Next, the A549 cells were transfected with siRNA against BiP, which is a pivotal protein in the chemotherapy resistance of cancer cells. The expression levels of BiP decreased remarkably.

scholarly journals A supramolecular platform for controlling and optimizing molecular architectures of siRNA targeted delivery vehicles

2020 ◽  
Vol 6 (31) ◽  
pp. eabc2148
Author(s):  
Yuting Wen ◽  
Hongzhen Bai ◽  
Jingling Zhu ◽  
Xia Song ◽  
Guping Tang ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Targeted Delivery ◽  
Tumor Therapy ◽  
Sirna Delivery ◽  
Targeted Gene Delivery ◽  
Delivery Vehicles ◽  
Molecular Architectures ◽  
Host Polymer

It requires multistep synthesis and conjugation processes to incorporate multifunctionalities into a polyplex gene vehicle to overcome numerous hurdles during gene delivery. Here, we describe a supramolecular platform to precisely control, screen, and optimize molecular architectures of siRNA targeted delivery vehicles, which is based on rationally designed host-guest complexation between a β-cyclodextrin–based cationic host polymer and a library of guest polymers with various PEG shape and size, and various density of ligands. The host polymer is responsible to load/unload siRNA, while the guest polymer is responsible to shield the vehicles from nonspecific cellular uptake, to prolong their circulation time, and to target tumor cells. A series of precisely controlled molecular architectures through a simple assembly process allow for a rapid optimization of siRNA delivery vehicles in vitro and in vivo for therapeutic siRNA-Bcl2 delivery and tumor therapy, indicating the platform is a powerful screening tool for targeted gene delivery vehicles.

Characterization of Cationic Vector-Based Gene Delivery Vehicles Using Isothermal Titration and Differential Scanning Calorimetry

2003 ◽  
pp. 319-348 ◽  
Author(s):  
Brian A. Lobo ◽  
Sheila A. Rogers ◽  
Christopher M. Wiethoff ◽  
Sirirat Choosakoonkriang ◽  
Susan Bogdanowich-Knipp ◽  
...  
Keyword(s):  
Differential Scanning Calorimetry ◽  
Gene Delivery ◽  
Scanning Calorimetry ◽  
Delivery Vehicles ◽  
Gene Delivery Vehicles
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