scholarly journals The oxidative pathway of carbohydrate metabolism in Escherichia coli. 6. Adaptation of glucose 6-phosphate dehydrogenase to growth in complex media*

1959 ◽  
Vol 72 (3) ◽  
pp. 426-429 ◽  
Author(s):  
Dwight B. McNair Scott ◽  
Eileen Chu
Keyword(s):  
Escherichia Coli ◽  
Carbohydrate Metabolism ◽  
Complex Media ◽  
Oxidative Pathway ◽  
Glucose 6 Phosphate Dehydrogenase

scholarly journals Effect of dithiocyano-methane on hexose monophosphate pathway in the respiratory metabolism of Escherichia coli

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yanfeng Chen ◽  
Wenjie Ke ◽  
Huabin Qin ◽  
Siwei Chen ◽  
Limei Qin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Control Group ◽  
Respiratory Metabolism ◽  
Hexose Monophosphate ◽  
Hexose Monophosphate Pathway ◽  
Response To Stress ◽  
Glucose Decomposition ◽  
Decomposition Pathway ◽  
Glucose 6 Phosphate Dehydrogenase

Abstract This paper studied the inhibitory effects of dithiocyano-methane (DM) on the glucose decomposition pathway in the respiratory metabolism of Escherichia coli. We investigated the effects of DM on the activities of key enzymes (ATPase and glucose-6-phosphate dehydrogenase, G6PDH), the levels of key product (nicotinamide adenosine denucleotide hydro-phosphoric acid, NADPH), and gene expression in the hexose monophosphate pathway (HMP). The results showed that the minimum inhibitory concentration (MIC) and the minimum bactericide concentration (MBC) of DM against the tested strains were 5.86 mg/L and 11.72 mg/L, respectively. Bacteria exposed to DM at MIC demonstrated an increase in bacterial ATPase and G6PDH activities, NADPH levels, and gene expression in the HMP pathway compared to bacteria in the control group, which could be interpreted as a behavioral response to stress introduced by DM. However, DM at a lethal concentration of 10 × MIC affected glucose decomposition by inhibiting mainly the HMP pathway in E. coli.

scholarly journals Alcoholic Stem Extract ofCoscinium fenestratumRegulates Carbohydrate Metabolism and Improves Antioxidant Status in Streptozotocin–Nicotinamide Induced Diabetic Rats

2005 ◽  
Vol 2 (3) ◽  
pp. 375-381 ◽  
Author(s):  
I. R. Punitha ◽  
K. Rajendran ◽  
Arun Shirwaikar ◽  
Annie Shirwaikar
Keyword(s):  
Carbohydrate Metabolism ◽  
Antioxidant Status ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Glutathione Synthetase ◽  
Alcoholic Extract ◽  
Enzymatic Antioxidants ◽  
Stem Extract ◽  
Gluconeogenic Enzyme ◽  
Glucose 6 Phosphate Dehydrogenase

Alcoholic extract of the stems ofCoscinium fenestratum, a medicinal plant indigenous to India and Sri Lanka used in ayurveda and siddha medicine for treating diabetes, was studied for its carbohydrate metabolism effect and antioxidant status in streptozotocin–nicotinamide induced type 2 diabetic rats. Oral administration ofC. fenestratumstem extract in graded doses caused a significant increase in enzymatic antioxidants such as catalase, superoxide dismutase, glutathione synthetase, peroxidase, and glutathione peroxidase and in the nonenzymatic antioxidants ascorbic acid, ceruloplasmin and tocopherol. Effects of alcoholic extract on glycolytic enzymes such as glucose-6-phosphate dehydrogenase, lactate dehydrogenase and hexokinase showed a significant increase in their levels, whereas a significant decrease was observed in the levels of gluconeogenic enzyme, glucose-6-phosphatase and alanine aminotransferase in treated diabetic rats. Serum creatinine and urea levels also declined significantly. This investigation demonstrates significant antidiabetic activity ofC. fenestratum.

Spermatozoa of the giant octopus of the North Pacific Octopus dofleini martini

1970 ◽  
Vol 175 (1038) ◽  
pp. 63-68 ◽  
Keyword(s):  
Carbohydrate Metabolism ◽  
North Pacific ◽  
Sperm Head ◽  
Glycolytic Pathway ◽  
Striking Feature ◽  
Total Length ◽  
The North ◽  
Condensed Nucleus ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
The North Pacific

Spermatozoa of the giant octopus, as obtained from ruptured metre-long spermatophores, are only feebly motile. The total length of a spermatozoon is 1/2 mm, most of it due to the tail. The sperm-head, slim and oblong, is composed of a highly condensed nucleus and a cork-screw shaped acrosome. A striking feature, which distinguishes octopus spermatozoa from those of mammals, is the presence of a large amount of glycogen, concentrated mainly around the sperm-head. This, together with the occurrence of high phosphorylase and phosphoghlcomutase activity, indicates that glycogenolysis represents a pathway of carbohydrate metabolism in octopus spermatozoa. Both glucose-6-phosphate isomerase and glucose-6-phosphate dehydrogenase were demonstrated in extracts of prepared sperm- homogenates, which suggests that octopus spermatozoa may be capable of metabolizing glucose-6-phosphate along the oxidative as well as the glycolytic pathway.

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