scholarly journals Regulation of gluconeogenesis during exposure of young rats to hypoxic conditions

1971 ◽  
Vol 121 (2) ◽  
pp. 169-178 ◽  
Author(s):  
F. J. Ballard
Keyword(s):  
Steady State ◽  
Concentration Ratio ◽  
Redox State ◽  
Adenine Nucleotides ◽  
Redox Potentials ◽  
Air Content ◽  
Hypoxic Conditions ◽  
Air Atmosphere ◽  
Old Rats ◽  
The Difference

1. Two-day-old rats were exposed at constant temperature to atmospheres containing air and nitrogen with the air content varied in steps from 100 to 0%. By using this system of graded hypoxia a comparison was made between rates of gluconeogenesis from lactate, serine and aspartate in the whole animal and the concentrations of several liver metabolites. 2. Gluconeogenesis, expressed as the percentage incorporation of labelled isotope into glucose plus glycogen, proceeds linearly for 30min when the animals are incubated in a normal air atmosphere, but is completely suppressed if the atmosphere is 100% nitrogen. 3. Preincubation of animals for between 5 and 30min under an atmosphere containing 19% air results in the attainment of a new steady state with respect to gluconeogenesis and hepatic concentrations of ATP, ADP, AMP, lactate, pyruvate, β-hydroxybutyrate and acetoacetate. 4. When lactate (100μmol), aspartate (20μmol) or serine (20μmol) was injected, it was shown that the more severe the hypoxia the greater the depression of gluconeogenesis. Under conditions when gluconeogenesis was markedly inhibited there were no changes in the degree of phosphorylation of hepatic adenine nucleotides, but free [NAD+]/[NADH] ratios fell in both cytosol and mitochondrial compartments of the liver cell. 5. Measurements of total liver NAD+ and NADH showed that the concentrations of these nucleotide coenzymes changed less with anoxia, in comparison with the concentration ratio of free coenzymes. 6. Calculations showed that the difference in NAD+–NADH redox potentials between mitochondrial and cytosol compartments increased with the severity of hypoxia. 7. From the constancy of the concentrations of adenine nucleotides it is concluded that liver of hypoxic rats can conserve ATP by lowering the rate of ATP utilization for gluconeogenesis. Gluconeogenesis may be regulated in turn by the changes in mitochondrial and cytosol redox state.

The oxidized forms of cytochrome oxidase

1968 ◽  
Vol 46 (11) ◽  
pp. 1371-1379 ◽  
Author(s):  
G. R. Williams ◽  
R. Lemberg ◽  
M. E. Cutler
Keyword(s):  
Steady State ◽  
Cytochrome C ◽  
Cytochrome Oxidase ◽  
Dominant Species ◽  
Redox State ◽  
Electron Flow ◽  
Aerobic Oxidation ◽  
The Difference

Ferric cytochrome oxidase (Soret max. 418 mμ) and "oxygenated" cytochrome oxidase (Soret max. 428 mμ) carry the same number of oxidizing equivalents (two per heme a) available for the oxidation of ferrous cytochrome c. "Oxygenated" oxidase can be formed by the aerobic oxidation of a3+ + a32+CO and therefore the difference from ferric cytochrome oxidase resides in the a3 moiety of the enzyme. When reductant is added to ferric oxidase aerobically, a transient formation of a2+a33+ occurs but at later times the dominant species in the steady state is "oxygenated" oxidase. It is suggested that the reason for the inhibition of electron flow between a2+ and a33+ may be either in conformational restraints or in the redox state of the associated copper. No such block is apparent in the reduction of "oxygenated" oxidase.

scholarly journals Effects of Genotypes and Treatment on Oxygenscan Parameters in Sickle Cell Disease

Cells ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 811
Author(s):  
Camille Boisson ◽  
Minke A. E. Rab ◽  
Elie Nader ◽  
Céline Renoux ◽  
Celeste Kanne ◽  
...  
Keyword(s):  
Steady State ◽  
Sickle Cell Disease ◽  
Blood Cell ◽  
Sickle Cell ◽  
Red Blood Cell ◽  
Cell Disease ◽  
Oxygen Gradient ◽  
Acute Complication ◽  
Adults And Children ◽  
The Difference

(1) Background: The aim of the present study was to compare oxygen gradient ektacytometry parameters between sickle cell patients of different genotypes (SS, SC, and S/β+) or under different treatments (hydroxyurea or chronic red blood cell exchange). (2) Methods: Oxygen gradient ektacytometry was performed in 167 adults and children at steady state. In addition, five SS patients had oxygenscan measurements at steady state and during an acute complication requiring hospitalization. (3) Results: Red blood cell (RBC) deformability upon deoxygenation (EImin) and in normoxia (EImax) was increased, and the susceptibility of RBC to sickle upon deoxygenation was decreased in SC patients when compared to untreated SS patients older than 5 years old. SS patients under chronic red blood cell exchange had higher EImin and EImax and lower susceptibility of RBC to sickle upon deoxygenation compared to untreated SS patients, SS patients younger than 5 years old, and hydroxyurea-treated SS and SC patients. The susceptibility of RBC to sickle upon deoxygenation was increased in the five SS patients during acute complication compared to steady state, although the difference between steady state and acute complication was variable from one patient to another. (4) Conclusions: The present study demonstrates that oxygen gradient ektacytometry parameters are affected by sickle cell disease (SCD) genotype and treatment.

scholarly journals Identification of novel DNA hypermethylation of the adenylate kinase 5 promoter in colorectal adenocarcinoma

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Bokyung Ahn ◽  
Yang Seok Chae ◽  
Soo Kyung Lee ◽  
Moa Kim ◽  
Hyeon Soo Kim ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Cell Lines ◽  
Adenylate Kinase ◽  
Dna Methyltransferase ◽  
Colorectal Adenocarcinoma ◽  
Adenine Nucleotides ◽  
Inverse Correlation ◽  
Dna Hypermethylation ◽  
Normal Tissues ◽  
The Difference

AbstractAdenylate kinase 5 (AK5) belongs to the adenylate kinase family that catalyses reversible phosphate transfer between adenine nucleotides, and it is related to various energetic signalling mechanisms. However, the role of AK5 in colorectal cancer (CRC) has not been reported. In this study, AK5 was significantly hypermethylated in CRC compared to adjacent normal tissues (P < 0.0001) and normal tissues (P = 0.0015). Although the difference in mRNA expression was not statistically significant in all of them, the selected 49 cases of CRC tissues with AK5 hypermethylation with the cut off value of 40% showed a significant inverse correlation with mRNA expression (P = 0.0003). DNA methylation of AK5 promoter significantly decreased and AK5 expression recovered by 5-aza-2′-deoxycytidine, DNA methyltransferase inhibitor in CRC cell lines. In addition, AK5 promoter activity significantly decreased due to DNA methyltransferase, and it increased due to 5-aza. Moreover, AK5 regulated the phosphorylated AMPK and mTOR phosphorylation and inhibited the cell migration and cell invasion in CRC cell lines. Furthermore, low AK5 expression is associated with poor differentiation (P = 0.014). These results demonstrate that the AK5 promoter is frequently hypermethylated and induced methylation-mediated gene down-regulation. AK5 expression regulates AMPK/mTOR signalling and may be closely related to metastasis in colorectal adenocarcinoma.

On the steady-state solution of the M/G/2 queue

1979 ◽  
Vol 11 (01) ◽  
pp. 240-255 ◽  
Author(s):  
Per Hokstad
Keyword(s):  
Steady State ◽  
General Solution ◽  
Laplace Transform ◽  
Queue Length ◽  
Joint Distribution ◽  
Length Distribution ◽  
Steady State Solution ◽  
Queue Length Distribution ◽  
The Difference ◽  
Number Of Customers

The asymptotic behaviour of the M/G/2 queue is studied. The difference-differential equations for the joint distribution of the number of customers present and of the remaining holding times for services in progress were obtained in Hokstad (1978a) (for M/G/m). In the present paper it is found that the general solution of these equations involves an arbitrary function. In order to decide which of the possible solutions is the answer to the queueing problem one has to consider the singularities of the Laplace transforms involved. When the service time has a rational Laplace transform, a method of obtaining the queue length distribution is outlined. For a couple of examples the explicit form of the generating function of the queue length is obtained.

Kinetics of the cytochrome c oxidase and reductase reactions in energized and de-energized mitochondria

1977 ◽  
Vol 55 (7) ◽  
pp. 706-713 ◽  
Author(s):  
Lars Chr. Petersen ◽  
Hans Degn ◽  
Peter Nicholls
Keyword(s):  
Steady State ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Oxygen Concentration ◽  
Respiration Rate ◽  
Redox State ◽  
Rat Liver Mitochondria ◽  
Excess Oxygen ◽  
Succinate Oxidation ◽  
State Reduction

1. Coupled, cytochrome-c-depleted ('stripped') rat liver mitochondria reducing oxygen in the presence of exogenous cytochrome c, with succinate or ascorbate as substrates, show marked declines in the steady-state reduction of cytochrome c in excess oxygen on addition of uncouplers. Calculated ratios of maximal turnover in the uncoupled state and in the energized state for the cytochrome c oxidase (EC 1.9.3.1) reaction lie between 3 and 6, as obtained with reconstituted oxidase-containing vesicles. The succinate-cytochrome c reductase activity in such mitochondria shows a smaller response to uncoupler than that of the oxidase.2. The respiration rates of uncoupled mitochondria oxidizing ascorbate in the presence of added cytochrome c follow a Michaelis–Menten relationship with respect to oxygen concentration, in accordance with the pattern found previously with the solubilized oxidase. But succinate oxidation tends to give nonlinear concave-upward double-reciprocal plots of respiration rate against oxygen concentration, in accordance with the pattern found previously with intact uncoupled mitochondria.3. From simultaneous measurements of cytochrome c steady-state reduction, respiration rate, and oxygen concentration during succinate oxidation under uncoupled conditions it is found that at full reduction of cytochrome c, apparent Km for oxygen is 0.9 μM and the maximal oxidase (aa3) turnover is 400 s−1 (pH 7.4, 30 °C).4. The redox state of cytochrome c in uncoupled systems reflects a simple steady state; the redox state of cytochrome c in energized systems tends towards an equilibrium condition with the terminal cytochrome a3, whose apparent potential under these conditions is more negative than that of cytochrome c.

scholarly journals Phosphatidylcholine synthesis in the developing small intestine

1980 ◽  
Vol 186 (2) ◽  
pp. 399-403 ◽  
Author(s):  
P G Holtzapple ◽  
C M Starr ◽  
T Morck
Keyword(s):  
Small Intestine ◽  
Oleic Acid ◽  
Steady State ◽  
Adult Rats ◽  
Acyltransferase Activity ◽  
Acid Incorporation ◽  
Old Rats ◽  
Phosphatidylcholine Synthesis

1. Phosphatidylcholine synthesis in the foetal, newborn and adult small intestine of rats was studied by determination of cytidine diphosphocholine-1,2-diacylglycerocholine phosphotransferase (cholinephosphotransferase) and acyl-CoA-1-acyl-sn-glycerol-3-phosphocholine acyltransferase (lysophosphatidylcholine acyltransferase) activities and the incorporation of [1-14C]oleic acid into phosphatidylcholine. 2. Cholinephosphotransferase activity was low in foetal jejunum and ileum, increased 3-4 fold in the ileum by 6 days of age and by 12 days in the jejunum. Jejunal activity remained constant throughout weaning; ileal activity gradually decreased to values 25% of that of the jejunum. 3. Lysophosphatidylcholine acyltransferase activity was high in foetal jejunum and ileum, decreased 70% immediately after birth in the jejunum and increased to adult values by 12 days of age. Ileal activity decreased by 20% after birth, but decreased more rapidly at weaning to 30% of the activity in jejunum. 4. Initial rates and steady-state incorporation of [1-14C]oleic acid into phosphatidylcholine by jejunal rings of 10 day-old rats exceeded that observed in jejunal rings from adult rats by 2-4-fold. 5. In the postnatal jejunum, neither cholinephosphotransferase and lysophosphatidylcholine acyltransferase activities nor oleic acid incorporation were stimulated by cortisone administration in vivo.

scholarly journals Inflammatory mediators in sickle cell anaemia highlight the difference between steady state and crisis in paediatric patients

2017 ◽  
Vol 182 (6) ◽  
pp. 933-936 ◽  
Author(s):  
Magda O. S. Carvalho ◽  
Théo Araujo-Santos ◽  
João H. O. Reis ◽  
Larissa C. Rocha ◽  
Bruno A. V. Cerqueira ◽  
...  
Keyword(s):  
Steady State ◽  
Sickle Cell ◽  
Inflammatory Mediators ◽  
Sickle Cell Anaemia ◽  
Paediatric Patients ◽  
The Difference

Effect of Saturation Degree on Concrete Deterioration due to Freeze-Thaw Action

2011 ◽  
Vol 477 ◽  
pp. 404-408 ◽  
Author(s):  
Wen Cui Yang ◽  
Yong Ge ◽  
Bao Sheng Zhang ◽  
Jie Yuan
Keyword(s):  
Frost Resistance ◽  
Water Pressure ◽  
High Water ◽  
Frost Damage ◽  
Critical Value ◽  
Cold Weather ◽  
Saturation Degree ◽  
Freeze Thaw ◽  
Air Content ◽  
The Difference

Freezing-thawing durability of cement concrete is extremely important in cold weather, to better understand mechanism of frost damage and air-entraining，saturation degree of pores in concrete and its relation with frost resistance were studied in this paper. Concrete specimens with different saturation degree from 0 to 100% were prepared used a sealed tin with a high water pressure pump. Then these specimens were subjected to six freezing-thawing cycles and the relative dynamic modulus of elasticity was examined. The results showed that critical saturation degree of concrete with water- binder ratio of 0.30 and 0.47, air content of 1%, 4% and 6% were from 0.60 to 0.80. When its saturation degree exceeded the critical value, concrete was deteriorated significantly after only six freeze-thaw cycles. The critical saturation degree was mainly related to the air content of concrete mixture, and it decreased with the increasing of air content. The difference between the saturation degree and the critical value can be used to evaluate potential frost resistance of concrete, and its result was consistent with the result of frost tests very well.

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