scholarly journals Testosterone control of nucleic acid content and proliferation of epithelium and stroma in rat seminal vesicles

1976 ◽  
Vol 160 (1) ◽  
pp. 43-48 ◽  
Author(s):  
S J Higgins ◽  
J M Burchell ◽  
W I Mainwaring
Keyword(s):  
Nucleic Acid ◽  
Acid Content ◽  
Seminal Vesicles ◽  
Male Rats ◽  
Nucleic Acid Content ◽  
Secretory Proteins ◽  
Tissue Weight ◽  
Wet Weight ◽  
Sexually Mature

Tissue wet weight, nucleic acid content and epithelial and stromal cell numbers were measured in the seminal vesicles of sexually mature male rats. After castration, tissue weight and RNA decreased rapidly and in aprallel to reach, after 14 days, values only 15-20% of those in control (not castrated) animals. During this period, DNA decreased to a much lesser extent (by about 40%), but this change in DNA correlates well with the observed loss of cells from the epithelium. Testosterone in vivo promoted an immediate resynthesis of RNA, the value characteristic of control animals being reached within 80h. Delays occurred in the hormone-induced regain of tissue weight (30h) and DNA (40h), each of which preceded proliferation of the epithelium (40-50h). The cells of the stroma were unaffected by these changes in the androgenic statls of the animal. It is suggested that these proliferative changes in the epithelium cannot account for the previously reported induction by testosterone of basic secretory proteins in this tissue.

IN VIVO EFFECTS OF HIGH-DOSE STEROIDS ON NUCLEIC ACID CONTENT OF IMMUNOCOMPETENT CELLS OF RENAL ALLOGRAFT RECIPIENTS

1988 ◽  
Vol 45 (3) ◽  
pp. 534-536
Author(s):  
A. J. Walle ◽  
G. Y. Wong ◽  
M. Suthanthiran ◽  
A. L. Rubin ◽  
K. H. Stenzel
Keyword(s):  
Nucleic Acid ◽  
Acid Content ◽  
Renal Allograft ◽  
Immunocompetent Cells ◽  
Nucleic Acid Content ◽  
High Dose ◽  
In Vivo Effects

scholarly journals Relative Growth, Nucleic Acid Content and Cell Numbers of the Brain in Octopus Vulgaris (Lamarck)

1970 ◽  
Vol 52 (3) ◽  
pp. 539-552
Author(s):  
A. PACKARD ◽  
V. ALBERGONI
Keyword(s):  
Nucleic Acid ◽  
Acid Content ◽  
Fold Increase ◽  
Small Cells ◽  
Nucleic Acid Content ◽  
Octopus Vulgaris ◽  
Homogeneous Population ◽  
Optic Lobes ◽  
Wet Weight ◽  
The Brain

1. The brain of Octopus vulgaris grows allometrically with respect to weight from 12 mg. at the beginning of post-larval life to 1.95 g. at a body weight of 8.5 kg. 2. The two optic lobes contribute 64% to the wet weight total at the beginning of the growth range and 44% at the end. 3. The 150-fo1d increase in brain weight is accompanied by a 40-fold increase in total DNA content, 80-85% of which is in the optic lobes. DNA concentrations of the order of 20 mg./g. wet weight are found in the vertical lobes. 4. An average of 1 mg. DNA is yielded by 1.9x108Octopus sperms and this figure is used to calibrate the nucleic acid content of the optic lobes, and of the suboesophageal and supraoesophageal divisions of the brain in terms of their ‘diploid equivalent’. 5. The ratio between the ‘diploid equivalent’ and the numbers of neurones counted histologically is highest for the suboesophageal division and lowest for the vertical lobes and probably indicates a high glia/neurone index in brain areas with many large cells and low index in those with a homogeneous population of small cells. The brain cells of O. vulgaris do not appear to be polyploid. 6. The RNA/DNA ratio is less than 1.0 in the optic and supraoesophageal lobes, higher than 1.0 in the suboesophageal lobes. The ratio increases during growth, especially in the suboesophageal division.

scholarly journals Impact of Curcumin on Nucleic Acids in Diethanolamine Induced Toxicity in Mus musculus

2021 ◽  
Vol 69 (2) ◽  
Author(s):  
Hetal Doctor ◽  
Sanman Samova ◽  
R.J. Verma
Keyword(s):  
Body Weight ◽  
Nucleic Acid ◽  
Acid Content ◽  
Nucleic Acid Content ◽  
High Dose ◽  
Dependent Manner ◽  
In Vivo Experiments ◽  
Ameliorative Effect ◽  
Dose Dependent

Tremendous hike in the use of chemicals in every consumables worldwide, led researchers to investigate the effects of various products and their ingredients that are being used in routine. Diethanolamine (DEA) is one such organic compound used in various industries and several personal care products that are being used daily. To evaluate DEA toxicity on nucleic acid content, Swiss strain male albino mice were chosen as animal model for in vivo experiments. Mice were exposed to DEA (110, 165, 330 mg/kg body weight/day) for 30 days. Animals were sorted into nine different groups, each containing 10 animals per group. In untreated control groups of animals no significant changes in nucleic acid content were noted whereas in DEA exposed animals, nucleic acid content decreased significantly (p<0.05) in a dose-dependent manner. However, more decrease were noted in high dose (330 mg/kg body weight/day) exposed animals as compared to other groups of animals. For the mitigation of the toxicity generated by DEA, curcumin, a miraculous antioxidant, which is an active component of turmeric was used. Curcumin (10, 20, 30 mg/kg body weight/day) was orally administered for 30 days along with the high dose of DEA. After completion of treatment, animals were humanly sacrificed and liver was quickly isolated for further biochemical evaluations. In animals exposed to curcumin for 30 days the nucleic acid content increased significantly (p<0.05) as compared to DEA-HD treated groups of animals. The effect was dose-dependent. The ameliorative effect of curcumin might be due to its high antioxidant potency.

METABOLISM AND MODE OF ACTION OF ANDROGENS IN TARGET TISSUES OF MALE RATS

1972 ◽  
Vol 69 (1) ◽  
pp. 165-173 ◽  
Author(s):  
H. Schmidt ◽  
I. Noack ◽  
K. D. Voigt
Keyword(s):  
Cell Proliferation ◽  
Cell Metabolism ◽  
Seminal Vesicles ◽  
Male Rats ◽  
Ventral Prostate ◽  
Nucleic Acid Content ◽  
The Difference ◽  
Independent Effects ◽  
Sites Of Action ◽  
Peripheral Metabolism

ABSTRACT The effect of testosterone and 5α-dihydrotestosterone on protein and nucleic acid content as well as on the activities of some enzymes has been studied in the ventral prostate and the seminal vesicles of immature castrated rats. Both androgens were given intraperitoneally in doses of 1 mg daily for one or three days the rats were sacrificed one day after the last injection. In the prostate it was found that 5α-dihydrotestosterone had a greater effect on DNA increase, i. e. cell proliferation than testosterone, whereas cell metabolism was stimulated by the two androgens to nearly the same extent. In the seminal vesicles a single dose led to the same results as had been obtained in the prostate, i. e. a greater cell proliferative action of 5α-dihydrotestosterone and an equal stimulation of cell metabolism by testosterone and 5α-dihydrotestosterone was also observed. When three doses of the two androgens were given, cell proliferation as well as cell metabolism in the seminal vesicles were significantly more increased after 5α-dihydrotestosterone than after testosterone. The difference of action after systemic administration of the two androgens is explained by their different accumulation and by their different peripheral metabolism in the target tissues. From the partly independent effects of various androgens on cell proliferation and cell metabolism the conclusion may be drawn that there exist at least two intracellular sites of action.

Application of Ogur & Rosen's Method for the Determination of Nucleic Acid Content of Bacteria

1957 ◽  
Vol 12 (2) ◽  
pp. 125-129 ◽  
Author(s):  
Nobuyasu KAWASAKI ◽  
Ichiro TAKI ◽  
Chiaki WATANABE ◽  
Kiyoshi MATOBA ◽  
Mokichiro NISHIO ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Acid Content ◽  
Nucleic Acid Content

scholarly journals Using flow cytometry and light-induced fluorescence technique to characterizethe variability and characteristics of bioaerosols in springtime at Metro Atlanta, Georgia

2018 ◽  
Author(s):  
Arnaldo Negron ◽  
Natasha DeLeon-Rodriguez ◽  
Samantha M. Waters ◽  
Luke D. Ziemba ◽  
Bruce Anderson ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Nucleic Acid ◽  
Acid Content ◽  
Fungal Spores ◽  
Epifluorescence Microscopy ◽  
Nucleic Acid Content ◽  
Rain Event ◽  
Bacterial Cells ◽  
Sampling System ◽  
Spores And Pollen

Abstract. The abundance and speciation of primary biological aerosol particles (PBAP) is important for understanding their impacts on human health, cloud formation and ecosystems. Towards this, we have developed a protocol for quantifying PBAP collected from large volumes of air with a portable wet-walled cyclone bioaerosol sampler. A flow cytometry (FCM) protocol was then developed to quantify and characterize the PBAP populations from the sampler, which were confirmed against epifluorescence microscopy. The sampling system and FCM analysis were used to study PBAP in Atlanta, GA over a two-month period and showed clearly defined populations of DNA-containing particles: Low Nucleic Acid-content particles (bioLNA), High Nucleic Acid-content particles (HNA) being fungal spores and pollen. We find that daily-average springtime PBAP concentration (1 to 5 μm diameter) ranged between 1.4 × 104 and 1.1 × 105 m−3. The BioLNA population dominated PBAP during dry days (72 ± 18 %); HNA dominated the PBAP during humid days and following rain events, where HNA (e.g., wet-ejected fungal spores) comprised up to 92 % of the PBAP number. Concurrent measurements with a Wideband Integrated Bioaerosol Sensor (WIBS-4A) showed that FBAP and total FCM counts are similar; HNA (from FCM) significantly correlated with ABC type FBAP concentrations throughout the sampling period (and for the same particle size range, 1–5 μm diameter). However, the FCM bioLNA population, possibly containing bacterial cells, did not correlate to any FBAP type. The lack of correlation of any WIBS FBAP type with the bioLNA suggest bacterial cells may be more difficult to detect with autofluorescence than previously thought. Ιdentification of bacterial cells even in the FCM (bioLNA population) is challenging, given that the fluorescence level of stained cells at times may be comparable to that seen from abiotic particles. HNA and ABC displayed highest concentration on a humid and warm day after a rain event (4/14), suggesting that both populations correspond to wet-ejected fungal spores. Overall, information from both instruments combined reveals a highly dynamic airborne bioaerosol community over Atlanta, with a considerable presence of fungal spores during humid days, and a bioLNA population dominating bioaerosol community during dry days.

Age-Related Effect of Dietary Nucleotides on Liver Nucleic Acid Content in Rats

1997 ◽  
Vol 41 (5) ◽  
pp. 324-330 ◽  
Author(s):  
Ana T. L&oacute;pez-Navarro ◽  
Angel Gil ◽  
Antonio S&aacute;nchez-Pozo
Keyword(s):  
Nucleic Acid ◽  
Acid Content ◽  
Nucleic Acid Content ◽  
Related Effect ◽  
Age Related ◽  
Dietary Nucleotides
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