HOXB5 induces invasion and migration through direct transcriptional up-regulation of β-catenin in human gastric carcinoma

HOXB5 is significantly up-regulated in gastric cancer and its expression is closely correlated with β-catenin expression. HOXB5 binds directly to the CTNNB1 promoter and activates its transcriptional expression, which in turn promotes invasion and migration activity of gastric cancer cells.

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MicroRNA-21 expression is associated with the clinical features of patients with gastric carcinoma and affects the proliferation, invasion and migration of gastric cancer cells by regulating Noxa

Molecular Medicine Reports ◽

10.3892/mmr.2016.4863 ◽

2016 ◽

Vol 13 (3) ◽

pp. 2701-2707 ◽

Cited By ~ 12

Author(s):

HAIBIN SUN ◽

PANZHI WANG ◽

QIANGNU ZHANG ◽

XIAOYAN HE ◽

GUOZHEN ZAI ◽

...

Keyword(s):

Gastric Cancer ◽

Gastric Carcinoma ◽

Cancer Cells ◽

Clinical Features ◽

Gastric Cancer Cells ◽

Invasion And Migration ◽

And Migration

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Effects and Mechanisms of Anlotinib and Dihydroartemisinin Combination Therapy in Ameliorating Malignant Biological Behavior of Gastric Cancer Cells

Current Pharmaceutical Biotechnology ◽

10.2174/1389201021666200623132803 ◽

2020 ◽

Vol 21 ◽

Author(s):

Qiong Luo ◽

Suyun Zhang ◽

Donghuan Zhang ◽

Rui Feng ◽

Nan Li ◽

...

Keyword(s):

Gastric Cancer ◽

Cancer Cells ◽

Molecular Mechanisms ◽

Chorioallantoic Membrane ◽

Cell Counting ◽

Biological Behavior ◽

Gastric Cancer Cells ◽

Invasion And Migration ◽

Apoptosis Related Protein ◽

And Migration

Background: Gastric cancer(GC) is currently one of the major malignancies that threatens human lives and health. Anlotinib is a novel small-molecule that inhibits angiogenesis to exert anti-tumor effects. However, the function in gastric cancer is incompletely understood. Objective: The aim of the present study was to investigate the anti-tumor effects and molecular mechanisms of anlotinib combined with dihydroartemisinin (DHA) in SGC7901 gastric cancer cells. Method: Different concentrations of anlotinib and DHA were used to treat SGC7901 gastric cancer cells, after which cell proliferation was measured. Drug interactions of anlotinib and DHA were analyzed by the Chou-Talalay method with CompuSyn software. proliferation, apoptosis, invasion, migration, and angiogenesis were measured using the cell counting kit-8 (CCK8) assay, flow cytometry, Transwell invasion assays, scratch assays, and chicken chorioallantoic membrane (CAM) assays. proliferation-associated protein (Ki67), apoptosis-related protein (Bcl-2), and vascular endothelial growth factor A (VEGF-A) were quantified by Western bloting. Results: The combination of 2.5 μmol/L of anlotinib and 5 of μmol/L DHA was highly synergistic in inhibiting cell growth, significantly increased the apoptosis rate and suppressed obviously the invasion and migration capability and angiogenesis of gastric cancer cells. In addition, the expression levels of Ki67, Bcl-2, and VEGF-A, as well as angiogenesis, were significantly decreased in the Combination of drugs compared with in control and either drug alone. Conclusion: The combination of anlotinib and DHA showed synergistic antitumor activity, suggesting their potential in treating patients with gastric cancer.

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