scholarly journals Lipid metabolism in microsomal fraction from photosynthetic tissue. Effects of catalase and hydrogen peroxide on oleate desaturation

1983 ◽  
Vol 213 (1) ◽  
pp. 249-252 ◽  
Author(s):  
D J Murphy ◽  
K D Mukherjee ◽  
E Latzko
Keyword(s):  
Hydrogen Peroxide ◽  
Lipid Metabolism ◽  
Pisum Sativum ◽  
Microsomal Fraction ◽  
Inhibitory Effect ◽  
Pisum Sativum L ◽  
Tissue Effects

On incubation of microsomal fraction from pea (Pisum sativum L.) leaves with ammonium [1-14C]oleate or [1-14C]oleoyl-CoA in the presence of ATP, CoA, Mg2+ and NADH, the major reactions observed were those catalysed by oleoyl-CoA synthetase, oleoyl-CoA thioesterase, oleoyl-CoA:phosphatidylcholine acyltransferase and oleoyl phosphatidylcholine desaturase. The reaction catalysed by oleoyl phosphatidylcholine desaturase was specifically inhibited by H2O2, and this inhibitory effect was overcome by catalase (EC 1.11.1.6).

scholarly journals EFFECT OF SALINE WATER AND POTASH FERTILIZER ON PROLINE CONTENT AND WATER POTENTAL IN PISUM SATIVUM L. (VAR.SENADOR CAMBADOS)

2007 ◽  
Vol 4 (3) ◽  
pp. 351-357
Author(s):  
Baghdad Science Journal
Keyword(s):  
Pisum Sativum ◽  
Water Potential ◽  
Irrigation Water ◽  
Saline Water ◽  
Inhibitory Effect ◽  
Proline Accumulation ◽  
Proline Content ◽  
Pisum Sativum L ◽  
Main Plot ◽  
Fertilizer Rates

Filed experiment was conducted to test the effect of saline water and potassium fertilizers rate on proline and water potential of Pisum sativum L. (Var.Senador Cambados ) leaves . Treatments of the experiment included two levels of water salinity( 2, 7 dSm-1) as a main plot and fertilizer rates as a sub plot. Results indicated that irrigation of plant with saline water 7 dSm-1 and fertilization 150 kg/donum increased proline accumulation and water potential 0.31 mmol/g,-17.00 bar at 9 AM morning and 0.62 mmol/g , -21.00 bar at 3 PM afternoon ,Irrigating plant with a 2 dSm-1 and fertilization 300 kg/donum decreased proline accumulation and water potential of leaves 0.22 mmol/g, -16.00 bar at 9 AM and 0.43 mmol/g,-18.00 bar at 3 PM . Irrigation plants with saline water 7 dSm-1 and fertilizer with 150 kg/ Donum K2SO4 increased Root/Shoot to 0.89 ,while 0.41 after irrigation with 2 dSm-1 saline water and fertilization with 300 kg/ Donum K2SO4 . The Na/K ratio increased to 0.53 under 7 dSm-1 of irrigation water and 150 kg/Donum fertilization and decreased to 0.1 under 2 dSm-1 irrigation water and fertilized 300 kg/ Donum . The results lead to the conclusion that potassium fertilization may reduce the inhibitory effect of increasing salinity of irrigation water on pea.

Allelopathic effects account for the inhibitory effect of field-pea (Pisum sativum L.) shoots on wheat growth in dense clay subsoils

2019 ◽  
Vol 55 (7) ◽  
pp. 649-659 ◽  
Author(s):  
Xiaojuan (Juan) Wang ◽  
Sale Peter ◽  
Zhiqian Liu ◽  
Roger Armstrong ◽  
Simone Rochfort ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Inhibitory Effect ◽  
Field Pea ◽  
Wheat Growth ◽  
Pisum Sativum L ◽  
Allelopathic Effects

scholarly journals Interaction of chronic ultraviolet radiation and cytokinin in adaptive reactions of pea plants

2020 ◽  
Vol 26 ◽  
pp. 196-201
Author(s):  
V. V. Zhuk ◽  
A. N. Mikheev ◽  
L. G. Ovsyannikova
Keyword(s):  
Hydrogen Peroxide ◽  
Pisum Sativum ◽  
Ultraviolet Radiation ◽  
Photosynthetic Pigments ◽  
Reactive Oxygen ◽  
Pisum Sativum L ◽  
Adaptive Reactions ◽  
Mass Accumulation

Aim. The goal of this study was to study the interaction of chronic ultraviolet (UV-B) radiation and cytokinin 6-benzylaminopurine (BAP) in the adaptation of pea plants (Pisum sativum L.) to the effect of chronic UV-B irradiation. Methods. Pea plants cultivar Aronis were sprayed by BAP solution in concentration 10-5 M before the effect of chronic UV-B radiation at doses of 6 kJ/m2 per day with a power of 1 W/m2 for 12 days. During this time, the growth of plants and their mass were measured, the content of photosynthetic pigments and endogenous hydrogen peroxide (HP) were determined in the leaves. Results. It was established that the effect of chronic UV-B radiation at a dose of 6 kJ/m2 per day on pea plants caused a delay in plant mass accumulation, synthesis of photosynthetic pigments and increasing HP content in leaves. Treatment plants by BAP increased mass, stimulated synthesis of photosynthetic pigments, reduced the content of HP in the leaves of pea plants during the UV-B radiation. Conclusions. It was shown that treatment of pea plants by BAP caused the adaptation of pea plants at the beginning of UV-B radiation. Cytokinins are capable to detoxify reactive oxygen spices, stimulate formation of photosynthetic complex that promotes growth of pea plants under chronic UV-B radiation. Keywords: UV-B, BAP, Pisum sativum L., photosynthetic pigments, adaptation.

scholarly journals The influence of selective light on the growth reaction and antioxidant system of seedlings Pisum sativum L.

2021 ◽  
Vol 29 ◽  
pp. 179-184
Author(s):  
O. O. Avksentieva ◽  
E.D. Batueva
Keyword(s):  
Hydrogen Peroxide ◽  
Pisum Sativum ◽  
Antioxidant System ◽  
Growth Response ◽  
The State ◽  
Aboveground Part ◽  
Pisum Sativum L ◽  
Etiolated Seedlings ◽  
Growth Reaction ◽  
Axial Organs

Aim. Study of the effect of red (660 nm), green (530 nm) and blue (450 nm) light on the growth processes and the state of the antioxidant system in the axial organs of seedlings of pea plants. Methods. Etiolated seedlings of pea Maecenat variety were irradiated with selective light with different spectrum of RL (660 nm), GL (530 nm), BL (450 nm) to activate photoreceptor systems of plants. In 10-day-old seedlings, growth response was determined – linear growth and biomass accumulation, as well as indicators of antioxidant system – hydrogen peroxide content and activity of oxidases – catalase and nonspecific peroxidase in axial organs of seedlings: in the aboveground part and roots. Results. Irradiation of the RL and the GL stimulates the accumulation of seedling biomass in the aboveground part and roots. BL inhibits the growth response of seedlings. The maximum stimulating effect is shown by the GL. The state of the antioxidant system in etiolated seedlings is characterized by organ specificity. Under the action of selective light, the content of the main form of ROS – hydrogen peroxide and shoots and in the roots, significantly stimulates the activity of catalase and peroxidase enzymes in the aboveground part of the seedling and is inhibited in the roots. The maximum effect in the aboveground part is shown by the GL, in the roots of the RL and the BL. Conclusions. The established effects of selective light irradiation are manifested differently in the aboveground and underground parts of seedlings. Possible mechanisms of connection of a condition of antioxidant system with separate aspects of signaling in photomorphogenesis of plants are discussed. Keywords: Pisum sativum L., selective light, RL (660 nm), GL (530 nm), BL (450 nm), growth reaction, axial organs, H2O2, catalase, peroxidase.

scholarly journals Substrate specificities of the enzymes of the oleate desaturase system from photosynthetic tissue

1985 ◽  
Vol 225 (1) ◽  
pp. 267-270 ◽  
Author(s):  
D J Murphy ◽  
I E Woodrow ◽  
K D Mukherjee
Keyword(s):  
Pisum Sativum ◽  
Microsomal Fraction ◽  
Major Product ◽  
Acyl Transfer ◽  
Functional Coupling ◽  
Substrate Specificities ◽  
Oleate Desaturase ◽  
Pisum Sativum L ◽  
Bulk Membrane ◽  
Desaturase System

In the microsomal fraction from young pea (Pisum sativum L.) leaves, the oleoyl moieties from oleoyl-CoA are principally transferred to the sn-2 position of phosphatidylcholine by oleoyl-CoA:1-acyl-lysophosphatidylcholine acyltransferase. The major product of this acyl transfer is 1-palmitoyl(stearoyl)-2-oleoyl phosphatidylcholine. The 1-palmitoyl(stearoyl)-2-oleoyl phosphatidylcholine is subsequently converted into 1-palmitoyl(stearoyl)-2-linoleoyl phosphatidylcholine by the oleate desaturase complex without equilibrating with the bulk membrane phosphatidylcholine pool. Hence, both the acyl transfer to phosphatidylcholine and the subsequent desaturation of oleoyl moieties occur on the sn-2 position of phosphatidylcholine, and there is also a functional coupling of the acyltransferase and oleate desaturase.

scholarly journals The control of CTP:choline-phosphate cytidylyltransferase activity in pea (Pisum sativum L.)

1986 ◽  
Vol 240 (3) ◽  
pp. 837-842 ◽  
Author(s):  
M J Price-Jones ◽  
J L Harwood
Keyword(s):  
Enzyme Activity ◽  
Pisum Sativum ◽  
Subcellular Distribution ◽  
Microsomal Fraction ◽  
Control Mechanisms ◽  
Stem Tissue ◽  
Enzyme Protein ◽  
Pisum Sativum L ◽  
Ylacetic Acid ◽  
Stimulation Of

Several possible control mechanisms for CTP:choline-phosphate cytidylyltransferase (EC 2.7.7.15) activity in pea (Pisum sativum L.) stems were investigated. Indol-3-ylacetic acid (IAA) treatment of the pea stems decreased total cytidylyltransferase activity but did not affect its subcellular distribution. Oleate (2 mM) caused some stimulation of enzyme activity by release of activity from the microsomal fraction into the cytosol, but neither phosphatidylglycerol nor monoacyl phosphatidylethanolamine had an effect on activity or subcellular distribution. A decrease in soluble cytidylyltransferase protein concentrations was found in IAA-treated pea stems, but this was not sufficient to account for all of the decrease in cytidylyltransferase activity. A 50% inhibition of enzyme activity could be obtained with 0.2 mM-CMP, which indicated possible allosteric regulation. Similar inhibition was obtained with 1.5 mM-ATP, but other nucleotides had no effect. The cytidylyltransferase enzyme protein was not directly phosphorylated, and the inhibition with 1.5 mM-ATP occurred with the purified enzyme, thus excluding an obligatory mediation via a modulator protein. The results indicate that the cytosolic form of cytidylyltransferase is the most important in pea stem tissue and that the decrease in cytidylyltransferase activity in IAA-treated material appears to be brought about by several methods.

scholarly journals Effect of Saline water and Potash Fertilizer on some chemical constituents in Pisum sativum L.(Var.Senador Cambados) plant.

2009 ◽  
Vol 6 (2) ◽  
pp. 257-264
Author(s):  
Baghdad Science Journal
Keyword(s):  
Pisum Sativum ◽  
Irrigation Water ◽  
Saline Water ◽  
Chemical Constituents ◽  
Inhibitory Effect ◽  
Water Salinity ◽  
Potash Fertilizer ◽  
Pisum Sativum L ◽  
Main Plot ◽  
Salinity Water

Field experiment was conducted to test the effect of saline water and fertilizers rate on Pisum sativum L. plants . Treatments of the experiment included two levels of water salinity (2, 7 dSm-1) as a main plot and three levels of potash fertilization K2SO4 (44%K) namely 150 control, 300 and 450 kg/Donum as a sub plot. Results indicated that irrigation of plant with saline water 7 dSm-¹ caused a significant decrease in K and P contents specially in the upper parts of the plants , the percentage of the K increased (2.80%) under 2 dSm-¹of irrigation water and 300 kg/ donum fertilizer rate in the upper leaves, However K decreased(1.10%) in lower leaves under 7 dSm-¹ and 300 kg/donum fertilizer. while P increased in pods under same water salinity but with 150kg/ donum potash fertilizer. although P decreased( 0. 3 %) in pods under 7 dSm-¹ salinity water with 150 kg/donum fertilizer. However the results showed irrigation with 7 dSm-¹ saline water and 150kg/ donum fertilizer increased Ca, Mg and Na percentage ( 3.1 ,0.45 ,1.19 % ) in the roots of plants respectively , the lowest Ca ,Mg percentage was in pods of plants irrigated with 7 dSm-¹ saline water and fertilized with 150kg/ donum (0.39,0.05%), beside Na lowest percentage was ( 0.16 %) in upper leaves after using 2 dSm-¹ saline water and fertilized with 300kg/ donum. Accordingly, conclusion could be made that potassium fertilization may reduce the inhibitory effect of increasing salinity of irrigation water on pea.

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