scholarly journals A new, highly sensitive method for the detection and quantification of penicillin-binding proteins

1993 ◽  
Vol 291 (1) ◽  
pp. 19-21 ◽  
Author(s):  
M Galleni ◽  
B Lakaye ◽  
S Lepage ◽  
M Jamin ◽  
I Thamm ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Binding Proteins ◽  
New Method ◽  
Bacterial Cells ◽  
Penicillin Binding Proteins ◽  
Naked Eye ◽  
Highly Sensitive ◽  
Direct Labelling ◽  
Sensitive Method ◽  
Detection And Quantification

A new method for the identification and quantification of penicillin-binding proteins is described which uses fluorescein-coupled penicillins. It allows the rapid detection of 0.2 pmol with the naked eye and 2 fmol with the help of an A.L.F. automatic DNA sequencer. Direct labelling can also be performed on whole bacterial cells.

Viral immunoblotting: A sensitive method for detecting viral-specific oliogoclonal bands in unconcentrated cerebrospinal fluid

1984 ◽  
Vol 4 (6) ◽  
pp. 505-510 ◽  
Author(s):  
S. Moyle ◽  
G. Keir ◽  
E. J. Thompson
Keyword(s):  
Cerebrospinal Fluid ◽  
Viral Antigen ◽  
New Method ◽  
Viral Antibodies ◽  
Highly Sensitive ◽  
Immunoprecipitation Reaction ◽  
Sensitive Method

A new method for detecting viral antibodies in cerebrospinal fluid is described. The technique has many advantages over previously published methods in that it is highly sensitive eliminating the need to concentrate the CSF, takes 5 h to complete, avoids the use of radionucleides, and most importantly circumvents problems associated with prozone effects which occur in immunoprecipitation reaction since the viral antigen is immobilized on nitrocellulose membranes.

Application of a new method for the sensitive detection and quantification of acyl-CoA esters in Arabidopsis thaliana seedlings and mature leaves

2000 ◽  
Vol 28 (6) ◽  
pp. 575-577 ◽  
Author(s):  
T. R. Larson ◽  
I. A. Graham
Keyword(s):  
Arabidopsis Thaliana ◽  
Reversed Phase ◽  
New Method ◽  
Sensitive Detection ◽  
Reversed Phase Hplc ◽  
Selective Method ◽  
Mature Leaves ◽  
Highly Sensitive ◽  
First Time ◽  
Detection And Quantification

We report a novel, highly sensitive and selective method for the detection and quantification of acyl-CoA esters from Arabidopsis thaliana. Extracted acyl-CoA esters were derived to their fluorescent acyl-etheno-CoA esters, separated by ion-paired reversed-phase HPLC, and detected fluorometrically. We report the composition and concentration of the acyl-CoA pool in mature A. thaliana leaves, and during storage-lipid breakdown in 2-day-old seedlings. The concentrations measured were in the 1–4 μM range for both tissue types. To our knowledge, this is the first time that reliable estimates of acyl-CoA ester concentrations have been made for plants.

XII.—On a New Method of Measurement of Minute Alternating Currents

1931 ◽  
Vol 50 ◽  
pp. 166-174 ◽  
Author(s):  
D. F. Martyn
Keyword(s):  
Magnetic Field ◽  
Alternating Current ◽  
New Method ◽  
Electro Magnetic Field ◽  
Highly Sensitive ◽  
Method Of Measurement ◽  
Electro Magnetic ◽  
Sensitive Method ◽  
Plate Current

SummaryA highly sensitive method of measuring alternating currents of the order of microamperes and of any frequency is described. The current to be measured is passed through the filament of a diode valve. The main heating current of this valve is supplied by an independent oscillator working at the frequency of the current under measurement. The change in the plate current of the valve is measured, thus indicating the amplitude of the alternating current being measured. Theoretical and practical details of the methods of working are given and particulars of the method by which the calibration was tested. It is suggested that the method is particularly applicable to the determination of electro-magnetic field strengths by measuring the currents induced in an aerial.

scholarly journals BOCILLIN FL, a Sensitive and Commercially Available Reagent for Detection of Penicillin-Binding Proteins

1999 ◽  
Vol 43 (5) ◽  
pp. 1124-1128 ◽  
Author(s):  
Genshi Zhao ◽  
Timothy I. Meier ◽  
Steven D. Kahl ◽  
Kyle R. Gee ◽  
Larry C. Blaszczak
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Rapid Detection ◽  
Binding Proteins ◽  
Uv Light ◽  
Rapid Determination ◽  
Penicillin G ◽  
Direct Evaluation ◽  
Penicillin Binding Proteins

ABSTRACT We describe a new, sensitive, rapid, and nonradioactive method involving the use of the commercially available BOCILLIN FL, a fluorescent penicillin, as a labeling reagent for the detection and study of penicillin-binding proteins (PBPs). This method allowed rapid detection of 30 ng of a purified PBP protein under UV light and of 2 to 4 ng of the protein with the aid of a FluorImager. This method also allowed rapid determination of the PBP profiles of Escherichia coli, Pseudomonas aeruginosa, and Streptococcus pneumoniae. The PBP profiles obtained are virtually identical to those reported previously with 3H-,14C-, or 125I-labeled penicillin. Using this method enabled us to determine the 50% inhibitory concentrations of the penicillin-sensitive and -resistant PBP2x proteins of S. pneumoniae for penicillin G, thereby allowing a direct evaluation of their relative affinities for penicillin G. Finally, this method also allowed us to compare relative affinities of a PBP2x protein for different β-lactam antibiotics with the aid of fluorescence polarization technology and to monitor a PBP2x protein during purification.

A fast and highly sensitive method for the detection of canine distemper virus by the naked eye

2015 ◽  
Vol 7 (6) ◽  
pp. 2264-2267 ◽  
Author(s):  
Caroline R. Basso ◽  
Claudia C. Tozato ◽  
João Pessoa A. Junior ◽  
Valber A. Pedrosa
Keyword(s):  
Gold Nanoparticle ◽  
Canine Distemper Virus ◽  
Low Cost ◽  
Canine Distemper ◽  
Naked Eye ◽  
Highly Sensitive ◽  
Sensitive Method

An easy, low cost method for the detection of canine distemper virus (CDV) that requires no instrumentation and uses gold nanoparticle-labeled antibodies is presented.

Effect of piperacillin on morphology and viability of gram-negative bacteria

1984 ◽  
Vol 42 ◽  
pp. 218-219
Author(s):  
R. H. Liss
Keyword(s):  
Inhibitory Concentration ◽  
Cytoplasmic Membrane ◽  
Drug Binding ◽  
Gram Negative Bacteria ◽  
Bacterial Cells ◽  
Drug Induced ◽  
Penicillin Binding Proteins ◽  
Lactam Antibiotic ◽  
Drug Free ◽  
Tube Dilution

Piperacillip (PIP) is b-[D(-)-α-(4-ethy1-2,3-dioxo-l-piperzinylcar-bonylamino)-α-phenylacetamido]-penicillanate. The broad spectrum semisynthetic β-lactam antibiotic is believed to effect bactericidal activity through its affinity for penicillin-binding proteins (PBPs), enzymes on the bacterial cytoplasmic membrane that control elongation and septation during cell growth and division. The purpose of this study was to correlate penetration and binding of 14C-PIP in bacterial cells with drug-induced lethal changes assessed by microscopic, microbiologic and biochemical methods.The bacteria used were clinical isolates of Escherichia coli and Pseudomonas aeruginosa (Figure 1). Sensitivity to the drug was determined by serial tube dilution in Trypticase Soy Broth (BBL) at an inoculum of 104 organisms/ml; the minimum inhibitory concentration of piperacillin for both bacteria was 1 μg/ml. To assess drug binding to PBPs, the bacteria were incubated with 14C-PIP (5 μg/0.09 μCi/ml); controls, in drug-free medium.

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