scholarly journals Cationic gas-filled microbubbles for ultrasound-based nucleic acids delivery

2017 ◽  
Vol 37 (6) ◽  
Author(s):  
Anthony Delalande ◽  
Colette Bastié ◽  
Lucie Pigeon ◽  
Simona Manta ◽  
Matthias Lebertre ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Electrostatic Interactions ◽  
Low Frequency ◽  
Target Tissue ◽  
High Concentration ◽  
Non Invasive ◽  
Efficient Delivery ◽  
Low Frequency Ultrasound ◽  
Nucleic Acids Delivery

The use of ultrasound has gained great interest for nucleic acids delivery. Ultrasound can reach deep tissues in non-invasive manner. The process of sonoporation is based on the use of low-frequency ultrasound combined with gas-filled microbubbles (MBs) allowing an improved delivery of molecules including nucleic acids in the insonified tissue. For in vivo gene transfer, the engineering of cationic MBs is essential for creating strong electrostatic interactions between MBs and nucleic acids leading to their protection against nucleases degradation and high concentration within the target tissue. Cationic MBs must be stable enough to withstand nucleic acids interaction, have a good size distribution for in vivo administration, and enough acoustic activity to be detected by echography. This review aims to summarize the basic principles of ultrasound-based delivery and new knowledge acquired in these recent years about this method. A focus is made on gene delivery by discussing reported studies made with cationic MBs including ours. They have the ability for efficient delivery of plasmid DNA (pDNA), mRNA or siRNA. Last, we discuss about the key challenges that have to be faced for a fine use of this delivery system.

scholarly journals i-GONAD (improved genome-editing via oviductal nucleic acids delivery), a convenient in vivo tool to produce genome-edited rats

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Shuji Takabayashi ◽  
Takuya Aoshima ◽  
Katsuya Kabashima ◽  
Kazushi Aoto ◽  
Masato Ohtsuka ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Genome Editing ◽  
Nucleic Acids Delivery

scholarly journals A Review of the Combination Therapy of Low Frequency Ultrasound with Antibiotics

2017 ◽  
Vol 2017 ◽  
pp. 1-14 ◽  
Author(s):  
Yun Cai ◽  
Jin Wang ◽  
Xu Liu ◽  
Rui Wang ◽  
Lei Xia
Keyword(s):  
Combination Therapy ◽  
Bacterial Infections ◽  
Bacterial Resistance ◽  
Low Frequency ◽  
Bactericidal Effect ◽  
Low Frequency Ultrasound ◽  
Biofilm Bacteria ◽  
Frequency Ultrasound

Single antimicrobial therapy has been unable to resist the global spread of bacterial resistance. Literatures of availablein vitroandin vivostudies were reviewed and the results showed that low frequency ultrasound (LFU) has a promising synergistic bactericidal effect with antibiotics against both planktonic and biofilm bacteria. It also can facilitate the release of antibiotics from medical implants. As a noninvasive and targeted therapy, LFU has great potential in treating bacterial infections. However, more in-depth and detailed studies are still needed before LFU is officially applied as a combination therapy in the field of anti-infective treatment.

Combined Low-Frequency Ultrasound and Streptokinase Intravascular Destruction of Arterial Thrombi In Vivo

2011 ◽  
Vol 37 (10) ◽  
pp. 1644-1652 ◽  
Author(s):  
Igor E. Adzerikho ◽  
Alexander G. Mrochek ◽  
Vladimir T. Minchenya ◽  
Vyacheslav V. Dmitriev ◽  
Anatoly I. Kulak
Keyword(s):  
Low Frequency ◽  
Low Frequency Ultrasound ◽  
Frequency Ultrasound

Targeting 5α-reductase with 99mTc labeled dutasteride derivatives for prostate imaging

2020 ◽  
Vol 108 (4) ◽  
pp. 305-313
Author(s):  
◽  
Syed Qaiser Shah
Keyword(s):  
Room Temperature ◽  
Target Tissue ◽  
Prostate Imaging ◽  
Non Invasive ◽  
Good Target ◽  
Target Ratio ◽  
First Time ◽  
Analogous Behavior

AbstractTo assess the suitability of 99mTc labeled 5α-reductase (5α-Rds) inhibitors for non-invasive targeting of prostate cancer (PCa) using Male Sprague Dawely Rat (MSDR) model. In this work, dutasteride (Cpd-1) a 5α-Rds inhibitor was derivatized to its dithiocarbamate analogue (Cpd-2) for subsequent synthesis of 99mTc(CO)3-dutasteride dithiocarbamate (Cpd-3) using tricarbonyl technique. To determine the structure of Cpd-3, for the first time a reference Re(CO)3-dutasteride dithiocarbamate (Cpd-4) was synthesized and characterized with NMR, ESIMS, HPLC and elemental analysis. HPLC was used to establish the identity of Cpd-3 using Cpd-4 as a reference standard. The suitability of Cpd-3 as a new 5α-Rds targeting agent was investigated, both in vitro and vivo. The Cpd-3 has shown ≥98 % in vitro stability at room temperature and was remained ≥90 % stable up to 6 h. In serum Cpd-3 has revealed an analogous behavior with a small decrease in stability after 16 h. High uptake (26.25 ± 1.10 %, after 4 h of i.v.) of Cpd-3 was observed in the prostate (target tissue) of MSDR model with reasonably good target to non-target ratio. Blocking the target site with excess Cpd-2 considerably decreased the uptake of Cpd-3 to 4.10 ± 0.75 % in PCa. High in vitro stability in saline and serum and in vivo uptake in prostate of MSDR model plausibly attracts the opportunity of using Cpd-3 as a novel radiopharmaceutical for non invasive targeting of prostate.

Low-Frequency Ultrasound in Medicine: An In Vivo Evaluation

2012 ◽  
Vol 61 (6) ◽  
pp. 1658-1663 ◽  
Author(s):  
Antonino S. Fiorillo ◽  
Domenico Grimaldi ◽  
Donatella Paolino ◽  
Salvatore A. Pullano
Keyword(s):  
Low Frequency ◽  
In Vivo Evaluation ◽  
Low Frequency Ultrasound ◽  
Frequency Ultrasound
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