scholarly journals Up-regulation of long non-coding RNA SNHG20 promotes ovarian cancer progression via Wnt/β-catenin signaling

2018 ◽  
Vol 38 (1) ◽  
Author(s):  
Shanyang He ◽  
Yunhe Zhao ◽  
Xiaoping Wang ◽  
Yalan Deng ◽  
Zhiyong Wan ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Progression ◽  
Target Gene ◽  
Biological Function ◽  
Downstream Target ◽  
Non Coding Rna ◽  
Downstream Target Gene ◽  
Long Non Coding Rna ◽  
Nucleolar Rna ◽  
Signaling Activity

Long non-coding RNA small nucleolar RNA host gene 20 (SNHG20) has been demonstrated to play crucial regulatory roles in many types of cancer. However, the biological function of long ncRNA (lncRNA) SNHG20 in ovarian cancer is still unclear. In the present study, we found that lncRNA SNHG20 was significantly increased in ovarian cancer. In addition, lncRNA SNHG20 knockdown suppressed the ovarian cancer progression, whereas overexpression of SNHG20 showed the opposite effects. Moreover, our results also revealed that lncRNA SNHG20 knockdown inhibited Wnt/β-catenin signaling activity by suppressing β-catenin expression and reversing the downstream target gene expression. Taken together, lncRNA SNHG20 plays an pivotal role in ovarian cancer progression by regulating Wnt/β-catenin signaling.

scholarly journals LncRNA GAS5 Suppresses Ovarian Cancer Progression by Targeting miR-96-5p/PTEN Axis

2020 ◽  
Author(s):  
Qian Dong ◽  
Xiaoran Long ◽  
Jie Cheng ◽  
Xia Yin ◽  
Wenjing Wang ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Progression ◽  
Target Gene ◽  
Growth Arrest ◽  
Mtor Signaling Pathway ◽  
Downstream Target ◽  
Specific Transcript ◽  
Downstream Target Gene ◽  
Non Coding Rnas ◽  
Proliferation And Invasion

Abstract Background Long non-coding RNAs (lncRNA) play critical roles in tumor occurrence and progression, including ovarian cancer (OC). The lncRNA growth arrest-specific transcript 5 (GAS5) has been proved to be an important modulator in the growth and metastasis of OC cells. Our studies confirm that GAS5 is down-regulated in OC; however, the potential molecular mechanism underlying it remains to be elucidated. Results In our study, we demonstrated that the expression levels of GAS5 and PTEN decreased, while miR-96-5p was up-regulated in ovarian cancer samples and cell lines compared with controls. PTEN is the downstream target gene of miR-96-5p. The up-regulation of GAS5 inhibited the expression of miR-96-5p, which directly targets PTEN. GAS5 overexpression can significantly reduce OC cell proliferation and invasion ability via suppression of miR-96-5p expression. PTEN/AKT/mTOR expression had a positive correlation with GAS5 expression. Moreover, miR-96-5p promoted OC progression by mediating PTEN/AKT/mTOR signaling pathway. Conclusion Our study identified GAS5 as a ceRNA which regulates the PTEN/AKT/mTOR axis through sponging miR-96-5p in OC.

scholarly journals Long non-coding RNA MAFG-AS1 promotes proliferation and metastasis of breast cancer by modulating STC2 pathway

2020 ◽  
Author(s):  
Shihao Di ◽  
Die Lu ◽  
Chunni Chen ◽  
Tianshi Ma ◽  
Zigui Zou ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Target Gene ◽  
Invasion And Metastasis ◽  
Cancer Proliferation ◽  
Downstream Target ◽  
Qrt Pcr ◽  
Non Coding Rna ◽  
Downstream Target Gene ◽  
Proliferation And Metastasis ◽  
Long Non Coding Rna

Abstract Objective Breast cancer is the most common cancer in Chinese women. A number of studies proposed that long non-coding RNA plays an essential role in the regulation of invasion and metastasis of various forms of malignancy, including lung cancer, gastric cancer and bladder cancer. In this study, a long non-coding RNA MAFG-AS1 was explored in detail to understand the significance in the etiology of breast cancer.Methods Quantitative reverse transcription PCR (qRT-PCR) was used to examine the expression level of LncRNA MAFG-AS1 in tissues and cell lines. The association of LncRNA MAFG-AS1 expression and the postoperative prognosis was analyzed by the Kaplan-Meier method and log-rank test. Cell proliferation was evaluated in vitro and in vivo . Transwell assays were performed to examine the cell migration. Cell cycle and apoptosis was evaluated by flowcytometry analysis. The downstream target gene STC2 of LncRNA MAFG-AS1 was screened using the microarray analysis, which was validated by qRT-PCR, functional analysis, and rescue experiment.Results Expression of LncRNA MAFG-AS1 in the breast cancer tissues was significantly higher than the precancerous lesions. Elevated expression level of LncRNA MAFG-AS1 was correlated to the larger GTV (gross tumor volume), negative expression of ER, PR, Her2, lymph node metastasis, and poor prognosis. The potency of breast cancer proliferation, invasion and metastasis was inhibited in the absence of LncRNA MAFG-AS1.Tumorigenic capacity of breast cancer cells was inhibited in the absence of LncRNA MAFG-AS1. The downstream target gene regulated by LncRNA MAFG-AS1 was screened out by gene chip technology, GO analysis and QRT-PCR ultimately. Disrupted STC2 suppressed the cell proliferation and metastasis when the level of LncRNA MAFG-AS1 elevated.Conclusion The LncRNA MAFG-AS1 triggers tumorigenesis in the breast cancer and regulates breast cancer proliferation and metastasis by modulating the downstream target gene STC2. Results from our study indicates that LncRNA MAFG-AS1 can be used.

scholarly journals Long non-coding RNA SDCBP2-AS1 delays the progression of ovarian cancer via microRNA-100-5p-targeted EPDR1

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Xiu Liu ◽  
Chanyuan Liu ◽  
Aijun Zhang ◽  
Qi Wang ◽  
Jiao Ge ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Progression ◽  
Expression Profiling ◽  
Migration And Invasion ◽  
Related Protein ◽  
Biological Functions ◽  
Interactive Analysis ◽  
Non Coding Rna ◽  
Non Coding Rnas ◽  
Long Non Coding Rna

Abstract Background Dysregulation of long non-coding RNAs has been implied to connect with cancer progression. This research was to decipher the mechanism of long non-coding RNA SDCBP2-AS1 in ovarian cancer (OC) through regulation of microRNA (miR)-100-5p and ependymin-related protein 1 (EPDR1). Methods LncRNA SDCBP2-AS1 and EPDR1 levels in OC were assessed by Gene Expression Profiling Interactive Analysis. lncRNA SDCBP2-AS1, miR-100-5p, and EPDR1 levels in OC tissues and cells were determined. SKOV3 and A2780 cells were transfected with lncRNA SDCBP2-AS1, miR-100-5p, and EPDR1-related plasmids or sequences, and then their functions in cell viability, apoptosis, migration, and invasion were evaluated. The interplay of lncRNA SDCBP2-AS1, miR-100-5p, and EPDR1 was clarified. Results LncRNA SDCBP2-AS1 and EPDR1 levels were suppressed whilst miR-100-5p level was elevated in OC. After upregulating lncRNA SDCBP2-AS1 or EPDR1, viability, migration, and invasion of OC cells were impaired, and apoptosis rate was increased. Downregulating EPDR1 or upregulating miR-100-5p partially mitigated upregulated lncRNA SDCBP2-AS1-induced impacts on the biological functions of OC cells. LncRNA SDCBP2-AS1 sponged miR-100-5p, and EPDR1 was targeted by miR-100-5p. Conclusion It is illustrated that lncRNA SDCBP2-AS1 regulates EPDR1 by sponge adsorption of miR-100-5p to inhibit the progression of OC.

scholarly journals Long non-coding RNA SNHG25 promotes epithelial ovarian cancer progression by up-regulating COMP

2021 ◽  
Vol 12 (6) ◽  
pp. 1660-1668
Author(s):  
Yinglei Liu ◽  
Boqun Xu ◽  
Manhua Liu ◽  
Haifeng Qiao ◽  
Siming Zhang ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Epithelial Ovarian Cancer ◽  
Cancer Progression ◽  
Non Coding Rna ◽  
Long Non Coding Rna

scholarly journals Knockdown of Long Non-coding RNA SNGH3 by CRISPR-dCas9 Inhibits the Progression of Bladder Cancer

2021 ◽  
Vol 8 ◽  
Author(s):  
Yu Cao ◽  
Qiong Hu ◽  
Ruiming Zhang ◽  
Ling Li ◽  
Mingjuan Guo ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Cancer Progression ◽  
Research Evidence ◽  
Histological Grade ◽  
Non Coding Rna ◽  
Clinical Relationship ◽  
Non Coding Rnas ◽  
Cancer Tissues ◽  
Long Non Coding Rna ◽  
Nucleolar Rna

Recent research evidence documents that lncRNAs (long non-coding RNAs lncRNAs) play a pivotal role in the tumorigenesis and development of tumors. LncRNA SNGH3 (small nucleolar RNA host gene 3) is highly expressed in numerous forms of cancer, serving as an oncogene in cancer progression. Nonetheless, the clinical relationship, along with the mechanism of SNGH3 in bladder cancer, have not been studied. Herein, the findings exhibited upregulation of SNGH3 in bladder cancer tissues, along with the cell lines. Furthermore, overexpressed SNGH3 was positively linked to the TNM stage, as well as the histological grade of bladder cancer. Moreover, the silencing of SNGH3, using CRISPR-dCas9, suppressed cell growth along with migration, but elevated bladder cancer cell apoptosis. In summary, we established that SNGH3 serves as a bladder cancer oncogene and could be employed as a prospective diagnostic marker for clinical use, and is also a therapeutic target for CRISPR-mediated gene therapy.

scholarly journals Long non-coding RNA FLJ33360 participates in ovarian cancer progression by sponging miR-30b-3p

2019 ◽  
Vol Volume 12 ◽  
pp. 4469-4480 ◽  
Author(s):  
Meiqin Yang ◽  
Zhensheng Zhai ◽  
Shuang Guo ◽  
Xiaoxi Li ◽  
Yongxia Zhu ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Progression ◽  
Non Coding Rna ◽  
Long Non Coding Rna
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