A meta-analysis of the activity, stability, and mutational characteristics of temperature-adapted enzymes

Understanding the characteristics that define temperature-adapted enzymes has been a major goal of extremophile enzymology in recent decades. In this study, we explore these characteristics by comparing psychrophilic, mesophilic, and thermophilic enzymes. Through a meta-analysis of existing data, we show that psychrophilic enzymes exhibit a significantly larger gap (Tg) between their optimum and melting temperatures compared to mesophilic and thermophilic enzymes. These results suggest that Tg may be a useful indicator as to whether an enzyme is psychrophilic or not and that models of psychrophilic enzyme catalysis need to account for this gap. Additionally, by using predictive protein stability software, HoTMuSiC and PoPMuSiC, we show that the deleterious nature of amino acid substitutions to protein stability increases from psychrophiles to thermophiles. How this ultimately affects the mutational tolerance and evolutionary rate of temperature adapted organisms is currently unknown.

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Meta-Analysis—Correlation between Spiral Ganglion Cell Counts and Speech Perception with a Cochlear Implant

Audiology Research ◽

10.3390/audiolres11020020 ◽

2021 ◽

Vol 11 (2) ◽

pp. 220-226

Author(s):

Yew-Song Cheng ◽

Mario A. Svirsky

Keyword(s):

Speech Perception ◽

Cochlear Implant ◽

Ganglion Cells ◽

Meta Analysis ◽

Spiral Ganglion ◽

Spiral Ganglion Cell ◽

Cell Counts ◽

Temporal Bones ◽

The Relationship ◽

Existing Data

The presence of spiral ganglion cells (SGCs) is widely accepted to be a prerequisite for successful speech perception with a cochlear implant (CI), because SGCs provide the only known conduit between the implant electrode and the central auditory system. By extension, it has been hypothesized that the number of SGCs might be an important factor in CI outcomes. An impressive body of work has been published on findings from the laborious process of collecting temporal bones from CI users and counting the number of SGCs to correlate those numbers with speech perception scores, but the findings thus far have been conflicting. We performed a meta-analysis of all published studies with the hope that combining existing data may help us reach a more definitive conclusion about the relationship between SGC count and speech perception scores in adults.

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Meta-Analysis Combining New and Existing Data Sets Confirms that the TERT–CLPTM1L Locus Influences Melanoma Risk

Journal of Investigative Dermatology ◽

10.1038/jid.2011.322 ◽

2012 ◽

Vol 132 (2) ◽

pp. 485-487 ◽

Cited By ~ 35

Author(s):

Matthew H. Law ◽

Grant W. Montgomery ◽

Kevin M. Brown ◽

Nicholas G. Martin ◽

Graham J. Mann ◽

...

Keyword(s):

Meta Analysis ◽

Data Sets ◽

Melanoma Risk ◽

Existing Data

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Childhood infections, orchitis and testicular germ cell tumours: a report from the STEED study and a meta-analysis of existing data

British Journal of Cancer ◽

10.1038/bjc.2012.45 ◽

2012 ◽

Vol 106 (7) ◽

pp. 1331-1334 ◽

Cited By ~ 4

Author(s):

B Trabert ◽

B I Graubard ◽

R L Erickson ◽

K A McGlynn

Keyword(s):

Germ Cell ◽

Meta Analysis ◽

Germ Cell Tumours ◽

Testicular Germ Cell ◽

Testicular Germ Cell Tumours ◽

Childhood Infections ◽

Existing Data

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Estimating drowning mortality in Tanzania: a systematic review and meta-analysis of existing data sources

Injury Prevention ◽

10.1136/injuryprev-2018-042939 ◽

2018 ◽

Vol 25 (5) ◽

pp. 459-471 ◽

Cited By ~ 2

Author(s):

Sophie Sarrassat ◽

Sigilbert Mrema ◽

Kassimu Tani ◽

Thomas Mecrow ◽

Dan Ryan ◽

...

Keyword(s):

Meta Analysis ◽

Complete Information ◽

Population Based ◽

Data Sources ◽

Prevention Strategies ◽

Policy Makers ◽

Drowning Prevention ◽

Study Sites ◽

Global Datasets ◽

Existing Data

BackgroundThe WHO advocates a 7-step process to enable countries to develop and implement drowning prevention strategies. We sought to assess, using existing data sources, the drowning situation in Tanzania as a first step in this process.MethodsWe searched for data on causes of death in Tanzania by reviewing existing literature and global datasets and by in-country networking. Authors and institutions were then contacted to request aggregate data on drowning mortality. Site-specific drowning estimates were combined using a random effects meta-analytic approach. We also tested for evidence of variations in drowning estimates by sex and by age group.ResultsWe acquired partial or complete information on drowning deaths for 13 data sources. We found strong evidence for substantial variations between study sites (p<0.001). Combining population-based data, we estimated an average of 5.1 drowning deaths per 100 000 persons per year (95% CI 3.8 to 6.3). The proportions of deaths due to drowning were 0.72% (95% CI 0.55 to 0.88) and 0.94% (95% CI 0.09 to 1.78) combining population-based data and hospital-based data, respectively. Males were at greater risk than females, while both under-five children and adults aged 45 years or more were at greater risk than those aged 5–44 years.ConclusionOur estimates of drowning burden are broadly in line with the 2016 Global Burden of Disease and the 2015 WHO Global Health Estimates. While this exercise was useful in raising the burden of drowning in Tanzania with policy makers, planning drowning prevention strategies in this country will require a better understanding of which subpopulations are at high risk.

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Amino-acid substitutions in a surface turn modulate protein stability

Natural Structural Biology ◽

10.1038/nsb0196-54 ◽

1996 ◽

Vol 3 (1) ◽

pp. 54-58 ◽

Cited By ~ 68

Author(s):

Paul F. Predki ◽

Vishal Agrawal ◽

Axel T. Brünger ◽

Lynne Regan

Keyword(s):

Amino Acid ◽

Protein Stability ◽

Amino Acid Substitutions

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Uwarunkowania wykluczenia emerytalnego z dodatkowej części systemu emerytalnego w Polsce

Ekonomia ◽

10.19195/2658-1310.27.2.1 ◽

2021 ◽

Vol 27 (2) ◽

pp. 9-25

Author(s):

Tomasz Jedynak

Keyword(s):

Economic Analysis ◽

Research Methods ◽

Critical Analysis ◽

Main Part ◽

Pension System ◽

Meta Analysis ◽

Economic Analysis Of Law ◽

Limited Availability ◽

Financial Exclusion ◽

Existing Data

The study addresses the issues of exclusion from the supplementary part of the Polish pension system. The main goals of the research are: 1) characterising retirement exclusion as a specific type of financial exclusion, and 2) identifying and assessing key determinants of retirement exclusion in the supplementary part of the Polish pension system. The article is epistemological and classifying in nature. In the review part, the research methods used are based on a critical analysis of the literature and a synthesis of its conclusions. The main part is based on a meta-analysis of the results of surveys regarding pension awareness and propensity to save, elements of the economic analysis of law, and analysis of existing data. The presented considerations lead to the formulation of arguments, that justify the thesis that the scale and extent of retirement exclusion in the supplementary part of the Polish pension system are a derivative of factors included in four meta-categories: 1. low pension awareness, 2. low saving potential, 3. low propensity to save, and 4. limited availability of pension products.

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Amino acid substitutions in CPC-LIKE MYB reveal residues important for protein stability in Arabidopsis roots

PLoS ONE ◽

10.1371/journal.pone.0205522 ◽

2018 ◽

Vol 13 (10) ◽

pp. e0205522

Author(s):

Koh Yamada ◽

Michiko Sasabe ◽

Yukichi Fujikawa ◽

Takuji Wada ◽

Rumi Tominaga-Wada

Keyword(s):

Amino Acid ◽

Protein Stability ◽

Amino Acid Substitutions

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Direct Oral Anticoagulants in the Treatment of Left Ventricular Thrombus: A Retrospective, Multicenter Study and Meta-Analysis of Existing Data

Journal of Cardiovascular Pharmacology and Therapeutics ◽

10.1177/1074248420967644 ◽

2020 ◽

pp. 107424842096764 ◽

Cited By ~ 2

Author(s):

John M. Cochran ◽

Xiaoming Jia ◽

Jessica Kaczmarek ◽

Kristen A. Staggers ◽

Mahmoud Al Rifai ◽

...

Keyword(s):

Controlled Trial ◽

Meta Analysis ◽

Vitamin K Antagonists ◽

Oral Anticoagulants ◽

Direct Oral Anticoagulants ◽

Left Ventricular ◽

Left Ventricular Thrombus ◽

Coronary Syndrome ◽

Ventricular Thrombus ◽

Existing Data

Aim: To compare the safety and efficacy of direct oral anticoagulants (DOAC) relative to vitamin K antagonists (VKA) for the treatment of left ventricular thrombus (LVT). Methods: This retrospective study enrolled patients diagnosed with LVT from 2014-2017. Patient characteristics and outcomes within 12 months of LVT diagnosis were recorded and analyzed. A meta-analysis was also performed by pooling our results with existing data in literature. Results: 14 DOAC and 59 VKA patients were included. Baseline demographic and clinical characteristics were similar except for age. Although more strokes within 12 months occurred in VKA (15%) than in DOAC (0%) patients, this was not statistically significant (p = 0.189). There were no significant differences in outcomes between patients on DOAC and VKA for acute coronary syndrome (ACS) (7%, vs 3.4%, p = .477), LVT resolution (86% vs 76%, p = .499) or bleeding (14% vs 14%, p = 1) within 12 months. The meta-analysis included 6 studies (n = 408 for DOACs; n = 1207 for VKA). There were no significant differences between DOACs versus VKAs with respect to odds for unresolved thrombus (OR 0.61, 95% CI 0.26,1.41), embolic events (OR 1.24, 95% CI 0.90,1.69), embolic events and death (OR 1.10, 95% CI 0.84,1.45) or bleeding events (OR 1.13, 95% CI 0.74,1.72). Conclusions: Our study and meta-analysis suggest similar efficacy and safety of DOACs in the treatment of LVT compared to VKA. These findings underscore the need for a randomized controlled trial.

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