Early accumulation of non-enzymatically synthesised oxylipins in Arabidopsis thaliana after infection with Pseudomonas syringae

2007 ◽  
Vol 34 (1) ◽  
pp. 65 ◽  
Author(s):  
Christoph Grun ◽  
Susanne Berger ◽  
Daniel Matthes ◽  
Martin J. Mueller
Keyword(s):  
Reactive Oxygen Species ◽  
Fatty Acids ◽  
Arabidopsis Thaliana ◽  
Jasmonic Acid ◽  
Pseudomonas Syringae ◽  
Virulent Strain ◽  
Reactive Oxygen ◽  
Hydroxy Fatty Acids ◽  
Avirulent Strain ◽  
Oxygen Species

The formation of non-enzymatic oxylipins is catalysed by reactive oxygen species. Reactive oxygen species are produced in response to pathogen attack. In this study, the accumulation of non-enzymatically formed hydroxy fatty acids and F1-phytoprostanes in leaves of Arabidopsis thaliana (L.) Heyhn upon infection with Pseudomonas syringae was investigated and compared with the accumulation of the enzymatically formed oxylipins jasmonic acid and 12-oxo-phytodienoic acid. Levels of all oxylipins increased after infection with a virulent and with an avirulent strain of P. syringae. Inoculation of the avirulent strain resulted in a biphasic accumulation with a first maximum around 5 h which was missing after inoculation of the virulent strain. Levels of free and esterified hydroxy fatty acids and F1-phytoprostanes increased after pathogen treatment; however, esterified compounds were 30 times more abundant than free oxylipins. The increase of the free compounds occurred later than the increase of the esterified compounds suggesting that non-enzymatic lipid oxidation occurs predominantly in membranes from which oxidised lipids can be released.

scholarly journals Extracts from cultures of Pseudomonas fluorescens induce defensive patterns of gene expression and enzyme activity while depressing visible injury and reactive oxygen species in Arabidopsis thaliana challenged with pathogenic Pseudomonas syringae

AoB Plants ◽  
2019 ◽  
Vol 11 (5) ◽  
Author(s):  
H Martin-Rivilla ◽  
A Garcia-Villaraco ◽  
B Ramos-Solano ◽  
F J Gutierrez-Mañero ◽  
J A Lucas
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Arabidopsis Thaliana ◽  
Pseudomonas Fluorescens ◽  
Pseudomonas Syringae ◽  
Reactive Oxygen ◽  
Signalling Pathway ◽  
Systemic Resistance ◽  
Oxygen Species ◽  
Ros Scavenging

Abstract We evaluated the ability of metabolic elicitors extracted from Pseudomonas fluorescens N21.4 to induce systemic resistance (ISR) in Arabidopsis thaliana against the pathogen Pseudomonas syringae DC3000. Metabolic elicitors were obtained from bacteria-free culture medium with n-hexane, ethyl acetate and n-butanol in three consecutive extractions. Each extract showed plant protection activity. The n-hexane fraction was the most effective and was used to study the signal transduction pathways involved by evaluating expression of marker genes of the salicylic acid (SA) signalling pathway (NPR1, PR1, ICS and PR2) and the jasmonic acid/ethylene (JA/ET) signalling pathway (PDF1, MYC2, LOX2 and PR3). In addition, the level of oxidative stress was tested by determining the activity of enzymes related to the ascorbate-glutathione cycle. N-hexane extracts stimulated both pathways based on overexpression of ICS, PR1, PR2, PDF1 and LOX2 genes. In addition, activity of the pathogenesis-related proteins glucanase (PR2) and chitinase (PR3), lipoxygenase and polyphenol oxidase was enhanced together with an increased capacity to remove reactive oxygen species (ROS). This was associated with less oxidative stress as indicated by a decrease in malondialdehyde (MDA), suggesting a causative link between defensive metabolism against P. syringae and ROS scavenging.

scholarly journals NTRC and Chloroplast-Generated Reactive Oxygen Species Regulate Pseudomonas syringae pv. tomato Disease Development in Tomato and Arabidopsis

2012 ◽  
Vol 25 (3) ◽  
pp. 294-306 ◽  
Author(s):  
Yasuhiro Ishiga ◽  
Takako Ishiga ◽  
Tamding Wangdi ◽  
Kirankumar S. Mysore ◽  
Srinivasa Rao Uppalapati
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Death ◽  
Pseudomonas Syringae ◽  
Function Analysis ◽  
Virulent Strain ◽  
Reactive Oxygen ◽  
Forward Genetics ◽  
Negative Regulator ◽  
Dependent Manner ◽  
Oxygen Species

Coronatine (COR)-producing pathovars of Pseudomonas syringae, including pvs. tomato, maculicola, and glycinea, cause important diseases on tomato, crucifers, and soybean, respectively, and produce symptoms with necrotic lesions surrounded by chlorosis. The chlorosis is mainly attributed to COR. However, the significance of COR-induced chlorosis in localized lesion development and the molecular basis of disease-associated cell death is largely unknown. To identify host (chloroplast) genes that play a role in COR-mediated chlorosis, we used a forward genetics approach using Nicotiana benthamiana and virus-induced gene silencing and identified a gene which encodes 2-Cys peroxiredoxin (Prxs) that, when silenced, produced a spreading hypersensitive or necrosis-like phenotype instead of chlorosis after COR application in a COI1-dependent manner. Loss-of-function analysis of Prx and NADPH-dependent thioredoxin reductase C (NTRC), the central players of a chloroplast redox detoxification system, resulted in spreading accelerated P. syringae pv. tomato DC3000 disease-associated cell death with enhanced reactive oxygen species (ROS) accumulation in a COR-dependent manner in tomato and Arabidopsis. Consistent with these results, virulent strain DC3000 suppressed the expression of Prx and NTRC in Arabidopsis and tomato during pathogenesis. However, interestingly, authentic COR suppressed the expression of Prx and NTRC in tomato but not in Arabidopsis, suggesting that COR in conjunction with other effectors may modulate ROS and cell death in different host species. Taken together, these results indicated that NTRC or Prx function as a negative regulator of pathogen-induced cell death in the healthy tissues that surround the lesions, and COR-induced chloroplast-localized ROS play a role in enhancing the disease-associated cell death.

scholarly journals Cell Wall Damage-Induced Lignin Biosynthesis Is Regulated by a Reactive Oxygen Species- and Jasmonic Acid-Dependent Process in Arabidopsis

2011 ◽  
Vol 156 (3) ◽  
pp. 1364-1374 ◽  
Author(s):  
Lucinda Denness ◽  
Joseph Francis McKenna ◽  
Cecile Segonzac ◽  
Alexandra Wormit ◽  
Priya Madhou ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Wall ◽  
Jasmonic Acid ◽  
Reactive Oxygen ◽  
Lignin Biosynthesis ◽  
Oxygen Species ◽  
Cell Wall Damage ◽  
Dependent Process

scholarly journals EFFECTS OF FATTY ACIDS ON PRODUCTION OF REACTIVE OXYGEN SPECIES (ROS) BY SKELETAL MUSCLE CELLS

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Renato Tadeu Nachbar ◽  
Augusto Ducati Luchessi ◽  
Tavane David Cambiaghi ◽  
Rafael Herling Lambertucci ◽  
Sandro Massao Hirabara ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Skeletal Muscle ◽  
Fatty Acids ◽  
Reactive Oxygen ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Oxygen Species

Effects of salicylic acid, jasmonic acid and reactive oxygen species on the resistance of Solanum peruvianum to Meloidogyne incognita

2021 ◽  
Vol 275 ◽  
pp. 109649
Author(s):  
Chong Du ◽  
Fengyi Shen ◽  
Yue Li ◽  
Zhentong Zhao ◽  
Xiangyang Xu ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Meloidogyne Incognita ◽  
Reactive Oxygen ◽  
Oxygen Species
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