Patterns of Total Peroxidase Activity and Isozyme Complement During Development of Selected Maturity Genotypes of Sorghum

Crop Science ◽  
1988 ◽  
Vol 28 (3) ◽  
pp. 531-535 ◽  
Author(s):  
Ching‐I Pao ◽  
Page W. Morgan
2012 ◽  
Vol 40 (1) ◽  
pp. 243 ◽  
Author(s):  
S. Filiz GUCLU ◽  
Fatma KOYUNCU

This study was carried out on 1-year old trees of ‘0900 Ziraat’ variety grafted onto ‘Kuş kirazi’, ‘Kara idris’, ‘Sari idris’, ‘MaxMa 14’, ‘MaxMa 60’ and ‘Gisela 5’ in order to determine their compatibility. For this purpose, total peroxidase activity was determined by spectrophotomery assay. Taking ground tissue samples were planned three times as before the grafting (beginning), and then 8 and 12 months after grafting. Total peroxidase activities of rootstocks before grafting varied between 10.80 ΔAg.min. (‘Kuş kirazi’) and 7.83 ΔAg.min. (‘Kara idris’) and were found to be statistically important. The peroxidase activity of ‘0900 Ziraat’ was 11.07ΔAg.min. and the closest value occurred in Prunus avium rootstock. The most different values occurred in rootstocks of ‘Kara idris’ and ‘Gisela 5’. The results showed that peroxidase activity increased in rootstock and graft scion. This increase had higher values in heterogenetic combinations especially in ‘0900 Ziraat’/‘Gisela 5’ and ‘0900 Ziraat’/‘Kara idris’ grafts. Peroxidase activity was decreased at the 12th month for all combinations. The highest value was obtained from ‘0900 Ziraat’/‘MaxMa 14’ combinaion with 29.17 ΔAg.min. while lowest one was ‘0900 Ziraat’/‘Kuş kirazi’ with 17.39. The findings showed that peroxidase activity could be used as a parameter in early determination of graft incompatibility.


Genome ◽  
1991 ◽  
Vol 34 (4) ◽  
pp. 495-504 ◽  
Author(s):  
M. A. Fieldes ◽  
J. Ross

The genetic regulation of the environmentally induced heritable difference in peroxidase activity between Durrant's large (L) and small (S) flax genotrophs was examined in leaves from plants ranging in developmental age from 6 days before anthesis to 3 days after. Mean peroxidase activity was higher for S than L and intermediate for the reciprocal F2's from L × S and S × L crosses (F2L × S and F2S × L). However, activity increased with development and, since there were small but significant differences in the average developmental ages of L, S, F2L × S, and F2S × L plants, the effects of development on activity had to be taken into account in examining the F2 activity data for segregation. A regression method was used to remove developmental effects and, underlying these effects, total peroxidase activity appeared to be regulated by a single locus with two alleles and L dominance. Two other dimorphic loci, both described previously, were also examined. One regulates the presence-absence of septa hairs in the seed capsules and the other the relative mobility of anionic peroxidase isozymes. There was no phenotypic linkage between the three segregating parameters. The genetic control of activity appeared to regulate cationic rather than anionic activity. In addition, a relationship between activity and plant height indicated either that peroxidase activity is one of the factors regulating main stem elongation or that the locus regulating peroxidase activity is linked to one of the loci involved in the regulation of plant height.Key words: flax genotrophs, peroxidase, genetic control, development.


1991 ◽  
Vol 69 (10) ◽  
pp. 2192-2196 ◽  
Author(s):  
R. Bronner ◽  
E. Westphal ◽  
F. Dreger

Peroxidase activity and its electrophoretic pattern were studied in resistant Solanum dulcamara leaves following infestation by the gall mite Aceria cladophthirus. Total peroxidase activity increased in infested leaves and was related to enhancement of three major peroxidase isozymes. The highest activity was found for the intercellular peroxidases. The extraction procedures used suggest that these peroxidases are pathogenesis-related proteins. These results are discussed with reference to the lack of lignin barrier around the mite-induced necrotic local lesions. Key words: Aceria cladophthirus, gall mite, Solanum dulcamara, resistant plant, pathogenesis-related proteins, peroxidases isozymes.


Plant Science ◽  
1999 ◽  
Vol 141 (1) ◽  
pp. 1-9 ◽  
Author(s):  
N Sreenivasulu ◽  
S Ramanjulu ◽  
K Ramachandra-Kini ◽  
H.S Prakash ◽  
H Shekar-Shetty ◽  
...  

1983 ◽  
Vol 10 (1) ◽  
pp. 87 ◽  
Author(s):  
F Gubler ◽  
AE Ashford

The effect of gibberellic acid (GA3) on peroxidase activity and isoenzyme complement was followed in isolated aleurone layers of barley (Hordeurn vulgare L.) over a 48-h period. A high level of peroxidase activity was initially present in freshly isolated aleurone layers. Disc electrophoresis resolved nine peroxidase isoenzymes. By 36 h incubation, total peroxidase activity had doubled in response to GA3 and two new peroxidase isoenzymes were observed. Peroxidase release from isolated aleurone layers was also greatly stimulated by GA3 and more isoenzymes were found in the medium. The time course of release was similar to that for α-amylase. Peroxidase activity in endosperm of whole germinating grains began to increase after 2 days, peaked at 4 days and subsequently declined.


2008 ◽  
Vol 133 (3) ◽  
pp. 315-319 ◽  
Author(s):  
Tao Wu ◽  
Jiashu Cao

Comparisons of total peroxidase activity and peroxidase isozymes as well as protein profiles among segregating, near-isogenic bush and vine phenotypes of pumpkin (Cucurbita moschata Duchesne) were investigated. Peroxidase activities of internode and leaf tissues of the bush plants were higher than those of respective vine tissues. Roots of bush plants, however, had a lower peroxidase activity than vine plants. In both bush and vine plants, peroxidase activities were lower in leaf tissues than in root and internode tissues. Electrophoretic comparisons revealed qualitative differences in peroxidase patterns in internodes between bush and vine plants. Moreover, qualitative differences between internode and root profiles were found between bush and vine plants in C. moschata. In conclusion, the results of this report revealed that a single gene conferring the bush phenotype in C. moschata might affect the relative expression of peroxidase activity, peroxidase isozymes, and protein profiles in leaf, internode, and root tissues.


2021 ◽  
Vol 19 (2) ◽  
pp. 101-108
Author(s):  
O.L. Nosareva ◽  
◽  
A.P. Pomogaeva ◽  
E.A. Stepovaya ◽  
T.V. Zhavoronok ◽  
...  

Objective. To assess the impact of changes in the content of ceruloplasmin and the activity of myeloperoxidase, superoxide dismutase, glutathione peroxidase, catalase on an increase in the severity and development of a form of pseudotuberculosis progression in children, to determine prognostic criteria for the formation of a non-smooth progression of the disease. Patients and methods. 125 children with pseudotuberculosis of various severity and progression were examined. The control group consisted of 45 children with health group II. The content of ceruloplasmin, the total peroxidase activity of blood plasma, the activity of superoxide dismutase, glutathione peroxidase, catalase, and myeloperoxidase were determined. Results. An increase in the activity of superoxide dismutase, myeloperoxidase and catalase in the acute period of pseudotuberculosis in all groups of children was determined. During the period of early convalescence an increase in the activity of superoxide dismutase, myeloperoxidase, and catalase against the background of comparable values of ceruloplasmin and glutathione peroxidase activity was observed in children with severe pseudotuberculosis. Conclusion. The results of the study prove that the mechanisms of non-smooth progression formation and an increase in the severity of pseudotuberculosis in children are mediated by an imbalance in the functioning of antiperoxides. A decrease in the activity of glutathione peroxidase due to an increase in the functioning of catalase in erythrocytes, no increase in the content of ceruloplasmin in the blood plasma during the acute period and the period of early convalescence serve as a prognostic criterion for the formation of a more severe degree and non-smooth progression of pseudotuberculosis. Key words: children, pseudotuberculosis, oxidative stress, antioxidant system


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