Poly lactic acid-caprolactone copolymer tube with a denatured skeletal muscle segment inside as a guide for peripheral nerve regeneration: A morphological and electrophysiological evaluation of the regenerated nerves

2003 ◽  
Vol 78 (3) ◽  
pp. 156-161 ◽  
Author(s):  
Nurru L. Mligiliche ◽  
Yasuhiko Tabata ◽  
Masaaki Kitada ◽  
Katsuaki Endoh ◽  
Keiko Okamato ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Lactic Acid ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Poly Lactic Acid

In vivo evaluation of poly(l-lactic acid) porous conduits for peripheral nerve regeneration

Biomaterials ◽  
1999 ◽  
Vol 20 (12) ◽  
pp. 1109-1115 ◽  
Author(s):  
G.R.D. Evans ◽  
K. Brandt ◽  
M.S. Widmer ◽  
L. Lu ◽  
R.K. Meszlenyi ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
In Vivo Evaluation

QUANTITATION OF NEUROTROPHIN mRNA IN SKELETAL MUSCLE: CHANGES DURING THE PROCESS OF PERIPHERAL NERVE REGENERATION

2006 ◽  
Vol 10 (03) ◽  
pp. 131-140 ◽  
Author(s):  
Yasushi Morisawa ◽  
Shinichiro Takayama ◽  
Kazuhiko Okushi ◽  
Toshiyasu Nakamura ◽  
Keiichi Fukuda ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Nerve Injury ◽  
High Performance ◽  
Peripheral Nerve Regeneration ◽  
Hplc Method ◽  
Mrna Levels ◽  
Muscle Changes ◽  
Over Time

Peripheral nerve injury changes the kinetics of neurotrophins. The production of several neurotrophins increases at the site of injury. Although numerous reports have described changes in neurotrophins over time in areas of nerve injury, neurotrophin mRNA is present at very low levels in target tissues, making accurate quantitation difficult. We developed a reverse transcription–polymerase chain reaction/high-performance liquid chromatography (RT-PCR/HPLC) method that enables accurate quantitation of neurotrophin mRNA. We then attempted to quantitate mRNA levels for nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) produced by skeletal muscle innervated by the sciatic nerve following transection and reattachment of the nerve in mice. In addition, wet weights of the muscle were measured and changes in weight over time were determined. The results indicated that neurotrophin production in muscle increases as a result of peripheral nerve denervation due to transection, and decreases with nerve regeneration and reinnervation resulting from reattachment.

scholarly journals Peripheral Nerve Regeneration Using a Cytokine Cocktail Secreted by Skeletal Muscle-Derived Stem Cells in a Mouse Model

2021 ◽  
Vol 10 (4) ◽  
pp. 824
Author(s):  
Daisuke Maki ◽  
Tetsuro Tamaki ◽  
Tsuyoshi Fukuzawa ◽  
Toshiharu Natsume ◽  
Ippei Yamato ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Mouse Serum ◽  
Facilitation Effect ◽  
Myelinated Fibers ◽  
Cytokine Cocktail

Severe peripheral nerve injury, which does not promise natural healing, inevitably requires clinical treatment. Here, we demonstrated the facilitation effect of peripheral nerve regeneration using a cytokine cocktail secreted by skeletal muscle-derived stem cells (Sk-MSCs). Mouse sciatic nerve was transected with a 6 mm gap and bridged collagen tube, and the culture supernatant of Sk-MSCs with 20% adult mouse serum (AMS)/Iscove’s modified Dulbecco’s medium (IMDM) was administered into the tube immediately after the operation, followed by an injection once a week for six weeks through the skin to the surrounding tube of the cytokine (CT) group. Similarly, 20% AMS/IMDM without cytokines was administered to the non-cytokine control (NT) group. Tension recovery in the plantar flexor muscles via electrical stimulation at the upper portion of the damaged nerve site, as well as the numerical recovery of axons and myelinated fibers at the damaged site, were evaluated as an index of nerve regeneration. Specific cytokines secreted by Sk-MSCs were compared with damaged sciatic nerve-derived cytokines. Six weeks after operation, significantly higher tension output and numerical recovery of the axon and myelinated fibers were consistently observed in the CT group, showing that the present cytokine cocktail may be a useful nerve regeneration acceleration agent. We also determined 17 candidate factors, which are likely included in the cocktail.

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