Quantitative determination of Streptococcus mutans by using competitive polymerase chain reaction

1999 ◽  
Vol 107 (2) ◽  
pp. 75-81 ◽  
Author(s):  
Stefan Rupf ◽  
Susanne Kneist ◽  
Knut Merte ◽  
Klaus Eschrich
Keyword(s):  
Polymerase Chain Reaction ◽  
Quantitative Determination ◽  
Streptococcus Mutans ◽  
Chain Reaction ◽  
Competitive Polymerase Chain Reaction ◽  
Polymerase Chain

Detection of potentially cariogenic strains of Streptococcus mutans using the polymerase chain reaction

2003 ◽  
Vol 27 (1) ◽  
pp. 47-51 ◽  
Author(s):  
Luis Alejandro Aguilera Galaviz ◽  
Ma. Del Carmen Aceves Medina ◽  
Iris Estrada García
Keyword(s):  
Polymerase Chain Reaction ◽  
Streptococcus Mutans ◽  
Oral Bacteria ◽  
Mutans Streptococci ◽  
Chain Reaction ◽  
Dmft Index ◽  
Biochemical Identification ◽  
Polymerase Chain ◽  
The Relationship

Streptococcus mutans is a pathogen related to the occurrence of human dental caries. The determination of total amounts of mutans streptococci, as well as the proportion related to other oral bacteria, is of interest when assessing the risk of developing caries. In this context, it is better to use a sensitive, specific and non-time consuming method such as the polymerase chain reaction (PCR), than to use culture and biochemical identification methods. In this work we identified potentially cariogenic strains of S. mutans and assessed the relationship with the dmft, DMFT or dmft/DMFT index. Using DNA isolated from dental plaque, a 192 bp sequence was identified and amplified from the spaP gene and a 722 bp sequence from the dexA gene. The results suggest that it is important to evaluate the presence of cariogenic S. mutans strains in plaque content rather than the accumulation of plaque itself. However, other factors like diet, hygiene, genetic background, the flow rate of saliva and the presence of specific antibodies, also play a key role in the development of caries.

Determination of plasma viral load in HIV-1 infection by quantitative competitive polymerase chain reaction

AIDS ◽  
1993 ◽  
Vol 7 ◽  
pp. S65-72 ◽  
Author(s):  
Michael Piatak ◽  
Michael S. Saag ◽  
Limei C. Yang ◽  
Steven J. Clark ◽  
John C. Kappes ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Viral Load ◽  
Plasma Viral Load ◽  
Chain Reaction ◽  
Competitive Polymerase Chain Reaction ◽  
Polymerase Chain ◽  
Hiv 1
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