The Mixed Lineage Kinase Leucine-Zipper Protein Kinase Exhibits a Differentiation-Associated Localization in Normal Human Skin and Induces Keratinocyte Differentiation upon Overexpression

Cyclophilin A, the major intracellular binding protein for the immunosuppressive drug cyclosporin A (CsA), was studied in human keratinocytes during differentiation both in vivo and in vitro. Analysis of cyclophilin by gel-filtration radiobinding-assay with tritiated CsA showed one specific radioactive peak at 17 kDa. By this technique, the levels of cyclophilin (mean 55.23 +/- 8.43 pmol/mg protein) did not significantly differ during keratinocyte differentiation. When the protein extracts from calcium-induced differentiating keratinocytes and normal human skin were analysed by PAGE radiobinding-assay, two specific radioactive CsA-binding peaks were detected. The major peak (RF 0.13) was expressed in all samples (mean 47.32 +/- 17.53 pmol/mg protein) whereas the minor peak (RF 0.23) was dramatically decreased about 6-fold in abnormally differentiated skin (psoriasis) as well as in non-differentiated keratinocytes. At least six [3H]CsA-binding isoforms with pI values ranging from 5.58 to 7.75 were detected by isoelectrofocusing autoradio-blotting-assay in normal human skin; three of them immunoreacted with antibodies to cyclophilin. These results demonstrated the presence of several cyclophilin isoforms in human epidermal cells and an expression which correlated with the differentiation of human keratinocytes both in vivo and in vitro.

Download Full-text

Immunohistochemical demonstration of novel protein kinase Cη in normal human skin and skin diseases

Journal of Dermatological Science ◽

10.1016/0923-1811(92)90152-2 ◽

1992 ◽

Vol 4 (2) ◽

pp. 123 ◽

Cited By ~ 2

Author(s):

Hiroko Koizumi ◽

Akira Ohkawara ◽

Toshio Kuroki

Keyword(s):

Protein Kinase ◽

Human Skin ◽

Skin Diseases ◽

Normal Human Skin ◽

Immunohistochemical Demonstration ◽

Normal Human ◽

Novel Protein

Download Full-text

Influence of Ispaghula seed husk polysaccharides on the gene expression of normal human skin keratinocytes (NHK)

Planta Medica ◽

10.1055/s-0030-1264534 ◽

2010 ◽

Vol 76 (12) ◽

Author(s):

A Deters

Keyword(s):

Gene Expression ◽

Human Skin ◽

Normal Human Skin ◽

Skin Keratinocytes ◽

Seed Husk ◽

Normal Human

Download Full-text

Clinical Studies on Hydrogen Ion Concentration on the Surface of Normal Human Skin

Nishi Nihon Hifuka ◽

10.2336/nishinihonhifu.31.296 ◽

1969 ◽

Vol 31 (3) ◽

pp. 296-314 ◽

Cited By ~ 1

Author(s):

Tadashi TAURA

Keyword(s):

Human Skin ◽

Clinical Studies ◽

Hydrogen Ion ◽

Ion Concentration ◽

Normal Human Skin ◽

Hydrogen Ion Concentration ◽

Normal Human

Download Full-text

Faculty Opinions recommendation of Tumor evolution. High burden and pervasive positive selection of somatic mutations in normal human skin.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.725509016.793507115 ◽

2015 ◽

Author(s):

William H Colledge

Keyword(s):

Positive Selection ◽

Human Skin ◽

Somatic Mutations ◽

Tumor Evolution ◽

Normal Human Skin ◽

High Burden ◽

Normal Human ◽

Selection Of

Download Full-text

The Effect of Practolol, In Vitro Generated Metabolites of Practolol and Chemical Analogues on the Rate of Growth of Human Skin Fibroblasts

Alternatives to Laboratory Animals ◽

10.1177/026119298401200205 ◽

1984 ◽

Vol 12 (2) ◽

pp. 89-97

Author(s):

Graham R. Elliott ◽

H.E. Amos ◽

James W. Bridges

Keyword(s):

Human Skin ◽

Psoriasis Patient ◽

Skin Fibroblasts ◽

Human Skin Fibroblasts ◽

Cell Type ◽

Normal Human Skin ◽

Rate Of Growth ◽

Structural Analogues ◽

Normal Human

The rate of growth of normal human skin fibroblasts was inhibited in a dose related, reversible, fashion by practolol (N-4-(2-hydroxy)-3 (1-methyl)-aminopropoxyphenylacetamine) (ID50 1.35 ± 0.14 x 10-3M), propranolol (1-(isopropylamino)-3(1-naphthyl-oxy)-2-propranolol) (ID50 0.145 ± 0.02 x 10-3M) and paracetamol (N-(4-hydroxyphenyl) acetamide) (ID50 0.85 ± 0.2 x 10-3M). Skin fibroblasts isolated from a psoriasis patient were more sensitive towards practolol (ID50 0.48 ± 0.14 x 10-3M) and propranolol (ID50 0.032 ± 0.002 x 10-3M), but less sensitive towards paracetamol (ID50 1.3 ± 0.07 x 10-3M). In vitro generated metabolites of practolol, using normal or Arochlor 1254-pretreated hamster liver preparations, and structural analogues of practolol had no effect upon the growth of either cell type.

Download Full-text