scholarly journals Biochemical synthesis of gold nanoparticles from leaf protein of Nicotiana tabacum L. cv. xanthi and their physiological, developmental, and ROS scavenging responses on tobacco plant under stress conditions

2018 ◽  
Vol 13 (1) ◽  
pp. 23-29 ◽  
Author(s):  
Syed Uzma Jalil ◽  
Manaal Zahera ◽  
Mohd Sajid Khan ◽  
Mohammad Israil Ansari
Keyword(s):  
Gold Nanoparticles ◽  
Nicotiana Tabacum ◽  
Tobacco Plant ◽  
Stress Conditions ◽  
Leaf Protein ◽  
Ros Scavenging ◽  
Nicotiana Tabacum L

scholarly journals Overexpression of chitinase gene with a GC-rich synthetic enhancer in tobacco plant (Nicotiana tabacum L.) Overekspresi gen kitinase dengan enhancer sintetis kaya GC pada tanaman tembakau (Nicotiana tabacum L.)

2016 ◽  
Vol 68 (2) ◽  
Author(s):  
D SANTOSO ◽  
T CHAIDAMSARI ◽  
A BUDIANI ◽  
H MINARSIH ◽  
S DWI UTOMO ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Nicotiana Tabacum ◽  
Transgenic Tobacco ◽  
Recombinant Dna ◽  
Tobacco Plant ◽  
Control Plant ◽  
Chitinase Activity ◽  
Dot Blot ◽  
Nicotiana Tabacum L ◽  
Hindiii Restriction Site

Ringkasan Perakitan tanaman perkebunan toleran terhadap serangan cendawan patogenik  dilakukan dengan mengoverekspresikan gen penyandi kitinase. Untuk itu elemen DNA peningkat ekspresi (enhancer E52) yang berupa oligonukleotida 52 pb dan kaya kandungan basa purin (GC) disisipkan di ujung 5’ konstruk 35S-chi. Penyisipan E52 tersebut dilakukan secara lebih terarah pada situs ganda HindIII-SalI dari MCS pCAMBIA2301. Melalui situs HindIII yang terletak tepat di ujung 3’ E52, konstruk 35S-chi kemudian disambungkan dengan E52 pada pCAMBIA tersebut. Transformasi DNA rekombinan ke dalam sel tembakau dikerjakan melalui perantaraan Agrobacterium tumefaciens LBA4404. Sel tanaman transgenik     diseleksi    dan  diregenrasi dalam media  yang    mengandung  3%  sukrosa, 0,5 mg/L diregenerasikan pada media   MS   padat  benzilaminopurin   (BAP)  dan  50 mg/L kanamisin. Pada media ini tunas transgenik    tembakau   mulai   terbentuk    setelah 5 minggu penanaman. Analisis  tingkat aktivitas enzimatis menunjukkan bahwa aktivitas kitinase pada tembakau transgenik 40 hingga 80 kali lebih tinggi daripada non-transgenik. Pengujian hibridisasi protein menggunakan antibodi anti-kitinase, dot blot dan western blot, membuktikan bahwa enhancer tersebut dapat meningkatkan ekspresi transgen chi pada tanaman tembakau.Summary Development of estate crops tolerant to pathogenic fungi is conducted by overexpressing chi gene. For this purpose, a synthetic enhancer consisting of 52 base pairs and GC-rich was inserted at immediate 5’ end of a 35S-chi cassette. Insertion of the E52 was directed at HindIII-SalI restriction sites of  the pCAMBIA2301 MCS.  With HindIII restriction site located just after the 3’ end of the E52 sequence, the 35S-chi construct was then ligated with the E52 of the pCAMBIA. Transformation of the resulting recombinant DNA into tobacco cells was mediated by Agrobacterium tumefaciens LBA4404. The transgenic cells were selected and regenerated on a solid MS medium supplemented with 3% sucrose, 0.5 mg/L benzylamino purine (BAP) and 50 mg/L kanamycin. Tobacco   shoots   were   initiated  after 5 weeks inoculation on the selection media. Enzymatic analysis demonstrated that chitinase activity of transgenic tobacco was 40 to 80 folds higher than that of the control plant. Analysis of  enzymatic activity using hybridization with anti-chitinase antibody indicated that the level of chitinase activity in the transgenic tobacco carrying the enhancer is higher than that  without enhancer. These data suggest that the enhancer improved the expression of chi transgene in tobacco. 

scholarly journals Overexpression of chitinase gene with a GC-rich synthetic enhancer in tobacco plant (Nicotiana tabacum L.) Overekspresi gen kitinase dengan enhancer sintetis kaya GC pada tanaman tembakau (Nicotiana tabacum L.)

2016 ◽  
Vol 68 (2) ◽  
Author(s):  
D SANTOSO ◽  
T CHAIDAMSARI ◽  
A BUDIANI ◽  
H MINARSIH ◽  
S DWI UTOMO ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Nicotiana Tabacum ◽  
Transgenic Tobacco ◽  
Recombinant Dna ◽  
Tobacco Plant ◽  
Control Plant ◽  
Chitinase Activity ◽  
Dot Blot ◽  
Nicotiana Tabacum L ◽  
Hindiii Restriction Site

Ringkasan Perakitan tanaman perkebunan toleran terhadap serangan cendawan patogenik  dilakukan dengan mengoverekspresikan gen penyandi kitinase. Untuk itu elemen DNA peningkat ekspresi (enhancer E52) yang berupa oligonukleotida 52 pb dan kaya kandungan basa purin (GC) disisipkan di ujung 5’ konstruk 35S-chi. Penyisipan E52 tersebut dilakukan secara lebih terarah pada situs ganda HindIII-SalI dari MCS pCAMBIA2301. Melalui situs HindIII yang terletak tepat di ujung 3’ E52, konstruk 35S-chi kemudian disambungkan dengan E52 pada pCAMBIA tersebut. Transformasi DNA rekombinan ke dalam sel tembakau dikerjakan melalui perantaraan Agrobacterium tumefaciens LBA4404. Sel tanaman transgenik     diseleksi    dan  diregenrasi dalam media  yang    mengandung  3%  sukrosa, 0,5 mg/L diregenerasikan pada media   MS   padat  benzilaminopurin   (BAP)  dan  50 mg/L kanamisin. Pada media ini tunas transgenik    tembakau   mulai   terbentuk    setelah 5 minggu penanaman. Analisis  tingkat aktivitas enzimatis menunjukkan bahwa aktivitas kitinase pada tembakau transgenik 40 hingga 80 kali lebih tinggi daripada non-transgenik. Pengujian hibridisasi protein menggunakan antibodi anti-kitinase, dot blot dan western blot, membuktikan bahwa enhancer tersebut dapat meningkatkan ekspresi transgen chi pada tanaman tembakau.Summary Development of estate crops tolerant to pathogenic fungi is conducted by overexpressing chi gene. For this purpose, a synthetic enhancer consisting of 52 base pairs and GC-rich was inserted at immediate 5’ end of a 35S-chi cassette. Insertion of the E52 was directed at HindIII-SalI restriction sites of  the pCAMBIA2301 MCS.  With HindIII restriction site located just after the 3’ end of the E52 sequence, the 35S-chi construct was then ligated with the E52 of the pCAMBIA. Transformation of the resulting recombinant DNA into tobacco cells was mediated by Agrobacterium tumefaciens LBA4404. The transgenic cells were selected and regenerated on a solid MS medium supplemented with 3% sucrose, 0.5 mg/L benzylamino purine (BAP) and 50 mg/L kanamycin. Tobacco   shoots   were   initiated  after 5 weeks inoculation on the selection media. Enzymatic analysis demonstrated that chitinase activity of transgenic tobacco was 40 to 80 folds higher than that of the control plant. Analysis of  enzymatic activity using hybridization with anti-chitinase antibody indicated that the level of chitinase activity in the transgenic tobacco carrying the enhancer is higher than that  without enhancer. These data suggest that the enhancer improved the expression of chi transgene in tobacco. 

scholarly journals Genomic Damage Induced in Nicotiana tabacum L. Plants by Colloidal Solution with Silver and Gold Nanoparticles

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1260
Author(s):  
Petra Lovecká ◽  
Anna Macůrková ◽  
Kamil Záruba ◽  
Tomáš Hubáček ◽  
Jakub Siegel ◽  
...  
Keyword(s):  
Dna Damage ◽  
Gold Nanoparticles ◽  
Nicotiana Tabacum ◽  
Colloidal Solution ◽  
Negative Control ◽  
Tail Moment ◽  
Tobacco Plants ◽  
Silver And Gold Nanoparticles ◽  
Nicotiana Tabacum L ◽  
Significant Difference

Tobacco seedlings (Nicotiana tabacum L cv. Wisconsin 38) were treated for 24 h with colloidal solution of silver and gold nanoparticles (AgNPs and AuNPs) of different size or cultivated for 8 weeks on soil polluted with these NPs. DNA damage in leaf and roots nuclei was evaluated by the comet assay. AgNPs of the size 22–25 nm at concentrations higher than 50 mg·L−1 significantly increased the tail moments (TM) values in leaf nuclei compared to the negative control. Ag nanoparticles of smaller size 12–15 nm caused a slight increase in tail moment without significant difference from the negative control. The opposite effect of AgNPs was observed on roots. The increasing tail moment was registered for smaller NPs. Similar results were observed for AuNPs at a concentration of 100 mg·L−1. DNA damaging effects after growing tobacco plants for 8 weeks in soil polluted with AgNPs and AuNPs of different size and concentrations were observed. While lower concentrations of both types of particles had no effect on the integrity of DNA, concentration of 30 mg·kg−1 of AgNPs caused significant DNA damage in leaves of tobacco plants. AuNPs had no effect even at the highest concentration. The content of Ag was determined by ICP–MS in above-ground part of plants (leaves) after 8 weeks of growth in soil with 30 mg·kg−1.AgNPs and was 2.720 ± 0.408 µg·g−1. Long term effect is much less harmful probably due to the plant restoration capability.

6-Substituted Indanoyl Isoleucine Conjugate Induces Tobacco Plant Responses in Secondary Metabolites

2005 ◽  
Vol 60 (1-2) ◽  
pp. 1-4 ◽  
Author(s):  
Qun Hu ◽  
Wilhelm Boland ◽  
Ji-Kai Liu
Keyword(s):  
Nicotiana Tabacum ◽  
Secondary Metabolites ◽  
Volatile Compounds ◽  
Secondary Metabolism ◽  
Tobacco Plant ◽  
Plant Responses ◽  
Plant Secondary Metabolism ◽  
Tobacco Leaves ◽  
Nicotiana Tabacum L

To characterize the role of the phytotoxin mimic 6-substituted indanoyl isoleucine conjugate 1 in plant secondary metabolism, tobacco (Nicotiana tabacum L. K326) was treated with compound 1. The volatile compounds of tobacco leaves were analyzed by GC-MS. In contrast to the control, three compounds, farnesene (2), santalol (3) and tetradecanal (4), were induced by treatment with 1 mm of compound 1. Concurrently other volatile compounds were also regulated.

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