scholarly journals Genomic Damage Induced in Nicotiana tabacum L. Plants by Colloidal Solution with Silver and Gold Nanoparticles

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1260
Author(s):  
Petra Lovecká ◽  
Anna Macůrková ◽  
Kamil Záruba ◽  
Tomáš Hubáček ◽  
Jakub Siegel ◽  
...  
Keyword(s):  
Dna Damage ◽  
Gold Nanoparticles ◽  
Nicotiana Tabacum ◽  
Colloidal Solution ◽  
Negative Control ◽  
Tail Moment ◽  
Tobacco Plants ◽  
Silver And Gold Nanoparticles ◽  
Nicotiana Tabacum L ◽  
Significant Difference

Tobacco seedlings (Nicotiana tabacum L cv. Wisconsin 38) were treated for 24 h with colloidal solution of silver and gold nanoparticles (AgNPs and AuNPs) of different size or cultivated for 8 weeks on soil polluted with these NPs. DNA damage in leaf and roots nuclei was evaluated by the comet assay. AgNPs of the size 22–25 nm at concentrations higher than 50 mg·L−1 significantly increased the tail moments (TM) values in leaf nuclei compared to the negative control. Ag nanoparticles of smaller size 12–15 nm caused a slight increase in tail moment without significant difference from the negative control. The opposite effect of AgNPs was observed on roots. The increasing tail moment was registered for smaller NPs. Similar results were observed for AuNPs at a concentration of 100 mg·L−1. DNA damaging effects after growing tobacco plants for 8 weeks in soil polluted with AgNPs and AuNPs of different size and concentrations were observed. While lower concentrations of both types of particles had no effect on the integrity of DNA, concentration of 30 mg·kg−1 of AgNPs caused significant DNA damage in leaves of tobacco plants. AuNPs had no effect even at the highest concentration. The content of Ag was determined by ICP–MS in above-ground part of plants (leaves) after 8 weeks of growth in soil with 30 mg·kg−1.AgNPs and was 2.720 ± 0.408 µg·g−1. Long term effect is much less harmful probably due to the plant restoration capability.

scholarly journals Lead Phytoremediation Potential of Wild Type and Transgenic Tobacco Plants

2021 ◽  
Vol 5 (1) ◽  
pp. 168-182
Author(s):  
Hatice DAGHAN ◽  
Veli UYGUR ◽  
Abdullah EREN
Keyword(s):  
Nicotiana Tabacum ◽  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Wild Type ◽  
Tobacco Plants ◽  
Nicotiana Tabacum L ◽  
Lead Phytoremediation

Genetiği değiştirilmiş bitkiler, kurşunun (Pb) kökten yer üstü kısımlarına translokasyonunu geliştirmek için büyük bir potansiyele sahip olabilir. Transgenik olmayan ( Nicotiana tabacum L. cv. Petit Havana SR1) ve transgenik (p-cV-ChMTII GFP) tütün bitkileri tarafından Pb alımının sağlanması araştırmak için Çin hamsteri metalotiyonin II gezen bir kap deneyi yapıldı . Transgenik ve transgenik olmayan tütün bitkileri, 0, 1000, 2500, 5000 mg Pb kg- 1 ile Pb (NO 3 ) 2 olarak işlenmiş topraklarda yetiştirildi. Kelimede bir büyüme bölümünde 6 hafta boyunca çiçeklenme aşamasına kadar.Bitkilerin büyümesi, klorofil içeriği, mineral besin elementleri ve düşük glutatyon (GSH) bezleri, bitkilerin Pb alım potansiyeli ile birlikte incelenmiştir. Hem transgenik hem de transgenik olmayan bitkiler için Pb uygulamasındaki artışa bağlı olarak yer üstü biyokütle çevrildi aşamalı bir düşüş gözlendi. Yaprak besinlerinin bulaştığı, aşırı Pb işlemlerinden olumsuz etkilenmiştir, bunlardan en büyük düşüşü. Sürgün Pb yüksek derecesi 76.0 mg kg kadar ulaşan -1 transgenik ve 70.9 mg kg -1 transgenik olmayan bitkilerde. Pb alımı, p-cV-ChMTII GFP'nin tütün bitkisine aktarılmasıyla iyileştirildi; ancak, Pb fitoremediasyonunda yeterli değildi. 

scholarly journals SMOKING GENOTOXICITY IN PERIPHERAL BLOOD LYMPHOCYTES USING THE COMET ASSAY

2013 ◽  
Vol 03 (03) ◽  
pp. 038-041
Author(s):  
Shobha S. Shetty ◽  
Hrishikesh Nachane
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
The Body ◽  
P Value ◽  
Tail Moment ◽  
Blood Lymphocytes ◽  
Significant Difference ◽  
Almost All

Abstract Background: Smoking has been shown to have a positive effect on DNA damage in almost all the cells of the body. Quantitative analysis of this damage will help in assessing the etiopathogenesis of various nicotine induced damage to the body. Comet assay has been an emerging tool in this regard and hence was applied by us to estimate the severity of DNA damage in smokers. Aims & Objectives: To evaluate the DNA genotoxicity in peripheral blood lymphocytes in smokers and their comparison with non smokers & assess the quantitative damage. Materials and methods: 30 smokers & 20 non smokers were recruited & their peripheral blood was taken for the comet assay to look for Olive moment & Tail moment to quantitatively assess the DNA damage due to cigarette smoking. Results: In our study there was no significant difference in the analysis of DNA damage (with regard to tail moment & olive moment) in smokers versus non smokers (P value: more than 0.05). Conclusions: Though smoking is known to cause DNA damage, we did not find significant differences between the two groups probably due to other multifactorial etiologies for genotoxicity.

scholarly journals Effects of photopolymerisation on genotoxicity of composite adhesives in the comet assay

Genetika ◽  
2016 ◽  
Vol 48 (2) ◽  
pp. 617-627
Author(s):  
Stefan Dacic ◽  
Ninoslav Djelic ◽  
Milena Radakovic ◽  
Nada Lakic ◽  
Aleksandar Veselinovic ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Human Lymphocytes ◽  
Negative Control ◽  
In Vivo Studies ◽  
Halogen Lamp ◽  
Genotoxic Effects ◽  
Significant Difference ◽  
Isolated Human Lymphocytes

Certain in vivo studies have shown that the application of adhesives directly onto the open pulp or on a thin layer of dentin causes inflammation and pulpal abscesses. This reaction is related to toxic effects of monomers from adhesives. It has been confirmed that after proper illumination the adhesives become less toxic. The aim of the study was to examine genotoxicity of non-polymerised, partly polymerised and polymerised adhesives on isolated human lymphocytes using the alkaline Comet assay. Adper Single bond2 and Adper Easy One/3M ESPE adhesive photopolymerisation was performed by Elipar Highlight 3M ESPE halogen lamp for 0, 10 and 40 sec, at final concentrations of 100, 200, 500 and 1000 ?g/mL. With both adhesives, photopolymerisation at 0 and 10 seconds showed statistically significant increase in DNA damage in comparision to the negative control (solvent). On the other hand, after 40 seconds of photopolymerisation of both adhesives in all tested concentrations, the degree of DNA damage in Comet assay had no significant difference (P>0.05, ?2 test) compared to the negative control. Therefore, only the 40 seconds of photopolymerisation prevented genotoxic effects of both adhesives in the Comet assay.

ACCUMULATION OF NITRATE AND CHLORIDE BY BURLEY TOBACCO

1974 ◽  
Vol 54 (1) ◽  
pp. 167-174 ◽  
Author(s):  
B. D. FUQUA ◽  
J. E. LEGGETT ◽  
J. L. SIMS
Keyword(s):  
Nicotiana Tabacum ◽  
The Other ◽  
Field Conditions ◽  
Leaf Cell ◽  
Plant Composition ◽  
Tobacco Plants ◽  
Burley Tobacco ◽  
Linear Relationships ◽  
Nicotiana Tabacum L ◽  
Root Media

Experiments were conducted to study the NO3− and Cl− interactions during uptake by burley tobacco (Nicotiana tabacum L., cult Ky 14) and the effects of their interactions on plant composition over long growth periods. Results indicated that NO3− or Cl− inhibited the absorption of the other anion by burley tobacco plants but did not influence the composition of cations. Positive linear relationships were found between NO3− and Cl− levels in solution, and the NO3− and Cl− contents in the plants in the laboratory and greenhouse studies. The inhibition of NO3− by Cl− during accumulation was also evident for tobacco plants grown under field conditions for longer periods. The interaction between NO3− and Cl− appears to be competitive during accumulation by burley tobacco. Competition between NO3− and Cl− during accumulation is proposed to be localized in the leaf cell rather than at the root–media interface.

Role of CaCl2 in nitrate assimilation in leaves and roots of tobacco plants (Nicotiana tabacum L.)

Plant Science ◽  
1999 ◽  
Vol 141 (2) ◽  
pp. 107-115 ◽  
Author(s):  
Juan M. Ruiz ◽  
Rosa M. Rivero ◽  
Pablo C. Garcia ◽  
Mourad Baghour ◽  
Luis Romero
Keyword(s):  
Nicotiana Tabacum ◽  
Nitrate Assimilation ◽  
Tobacco Plants ◽  
Nicotiana Tabacum L ◽  
Leaves And Roots
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