scholarly journals The pathogenetic aspects of acute intestinal infections of suckling piglets

2020 ◽  
Vol 17 ◽  
pp. 00104
Author(s):  
Alexey G. Shakhov ◽  
Larisa Yu. Sashnina ◽  
Julia Y. Vladimirova ◽  
Maria Y. Jeines ◽  
Anatoly Y. Samuilenko
Keyword(s):  
Molecular Weight ◽  
Total Protein ◽  
Immune Status ◽  
Diagnostic Value ◽  
Low Molecular Weight ◽  
Functional Reserve ◽  
Complex Process ◽  
Intestinal Infections ◽  
Intestinal Pathology ◽  
Suckling Piglets

According to the studies piglets with intestinal infections compared to the clinically healthy animals, have lower the content of total protein, gamma globulins and general immunoglobulins, higher levels of malondialdehyde and endogenous intoxication, low molecular weight circulating immune complexes, lysozyme and complementary blood serum activity, phagocytic activity of neutrophils, their absorption and metabolic ability with a low functional reserve of phagocytes. The revealed changes in the biochemical, antioxidant, and immune status should be considered one of the pathogenetic mechanisms of the complex process of inflammation, as well as their diagnostic value in case of intestinal pathology caused by bacteria.

scholarly journals Investigation of the N-terminal coding region of MUC7 alterations in dentistry students with and without caries

2016 ◽  
Vol 19 (1) ◽  
pp. 71-76 ◽  
Author(s):  
L Koç Öztürk ◽  
A Yarat ◽  
S Akyuz ◽  
H Furuncuoglu ◽  
K Ulucan
Keyword(s):  
Molecular Weight ◽  
Oral Hygiene ◽  
Total Protein ◽  
Turkish Population ◽  
Dental Students ◽  
Oral Bacteria ◽  
World Health ◽  
Low Molecular Weight ◽  
Coding Region ◽  
Protein Levels

ABSTRACTHuman low-molecular weight salivary mucin (MUC7) is a small, secreted glycoprotein coded by MUC7. In the oral cavity, they inhibit the colonization of oral bacteria, including cariogenic ones, by masking their surface adhesions, thus helping saliva to avoid dental caries. The N-terminal domain is important for low-molecular weight (MG2) mucins to contact with oral microorganisms. In this study, we aimed to identify the N-terminal coding region of the MUC7 gene between individuals with and without caries. Forty-four healthy dental students were enrolled in this study; 24 of them were classified to have caries [decayed, missing, filled-teeth (DMFT) = 5.6] according to the World Health Organization (WHO) criteria, and 20 of them were caries-free (DMFT = 0). Simplified oral hygiene index (OHI-S) and gingival index (GI) were used to determine the oral hygiene and gingival conditions. Total protein levels and salivary total protein levels and salivary buffer capacity (SBC) were determined by Lowry and Ericsson methods. DNA was extracted from peripheral blood cells of all the participants and genotyping was carried out by a polymerase chain reaction (PCR)-sequencing method. No statistical differences were found between two groups in the terms of salivary parameters, oral hygiene and gingival conditions. We detected one common single nucleotide polymorphism (SNP) that leads to a change of asparagine to lysine at codon 80. This substitution was found in 29.0 and 40.0%, respectively, of the groups with and without caries. No other sequence variations were detected. The SNP found in this study may be a specific polymorphism affecting the Turkish population. Further studies with extended numbers are necessary in order to clarify this finding.

Quantifying Serum Derived Differential Expressed and Low Molecular Weight Protein in Breast Cancer Patients

2020 ◽  
Vol 27 (7) ◽  
pp. 658-673
Author(s):  
Ayesha Zafar ◽  
Maryum Jabbar ◽  
Yasmeen Manzoor ◽  
Huma Gulzar ◽  
Shahzad Gul Hassan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Molecular Weight ◽  
Total Protein ◽  
Early Stage ◽  
Low Molecular Weight ◽  
Molecular Weight Protein ◽  
Early Detection Of Disease ◽  
Differential Protein ◽  
Weight Protein ◽  
Low Molecular Weight Protein

Background: Searching the biomarker from complex heterogeneous material for early detection of disease is a challenging task in the field of biomedical sciences. Objective: The study has been arranged to explore the proteomics serum derived profiling of the differential expressed and low molecular weight protein in breast cancer patient. Methods: Quantitative proteome was analyzed using the Nano LC/Mass and Bioinformatics tool. Results: This quantification yields 239 total protein constituting 29% of differentially expressed protein, with 82% downregulated differential protein and 18% up-regulated differential protein. While 12% of total protein were found to be cancer inducing proteins. Gene Ontology (GO) described that the altered proteins with 0-60 kDa mass in nucleus, cytosol, ER, and mitochondria were abundant that chiefly controlled the RNA, DNA, ATP, Ca ion and receptor bindings. Conclusion: The study demonstrate that the organelle specific, low molecular weighted proteins are significantly important biomarker. That act as strong agents in the prognosis and diagnosis of breast cancer at early stage.

scholarly journals Total protein, albumin and low-molecular-weight protein excretion in HIV-positive patients

2012 ◽  
Vol 13 (1) ◽  
Author(s):  
Lucy J Campbell ◽  
Tracy Dew ◽  
Rashim Salota ◽  
Emily Cheserem ◽  
Lisa Hamzah ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Total Protein ◽  
Hiv Positive ◽  
Low Molecular Weight ◽  
Molecular Weight Protein ◽  
Protein Excretion ◽  
Weight Protein ◽  
Low Molecular Weight Protein

scholarly journals Proteinuria in rats in relation to age-dependent renal changes

1980 ◽  
Vol 14 (2) ◽  
pp. 95-101 ◽  
Author(s):  
Jeannette M. Alt ◽  
H. Hackbarth ◽  
F. Deerberg ◽  
H. Stolte
Keyword(s):  
Molecular Weight ◽  
Total Protein ◽  
Plasma Proteins ◽  
Disc Electrophoresis ◽  
Male Rats ◽  
Low Molecular Weight ◽  
Urinary Proteins ◽  
Protein Excretion ◽  
Age Dependent ◽  
Renal Changes

A variety of sex-dependent urinary proteins of low molecular weight, absent in females and in castrated males, can be identified in male rats by disc electrophoresis. In the urine of male rats of age 5·5 months, albumin comprises only 1-2% of the total protein. Albumin excretion increases greatly with age and associated kidney disease. Total protein excretion, however, stays the same or even decreases slightly as the rat ages, due to a loss of low molecular weight, sex-dependent, proteins. These are virtually absent in senescent rats (38 months of age), although total protein excretion rises tenfold in these animals due to high molecular weight plasma proteins passing into the urine; the glomerular filtration rate decreases to 70% of the value measured at 5·5 months of age.

A high-performance oil-free backing pump for electron microscopes

1978 ◽  
Vol 36 (1) ◽  
pp. 110-111
Author(s):  
G.K.W. Balkau ◽  
E. Bez ◽  
J.L. Farrant
Keyword(s):  
Molecular Weight ◽  
Electron Microscope ◽  
Hot Spots ◽  
High Performance ◽  
Carbonaceous Material ◽  
Contamination Rate ◽  
Low Molecular Weight ◽  
Principal Source ◽  
Rotary Pumps ◽  
Electron Microscopes

The earliest account of the contamination of electron microscope specimens by the deposition of carbonaceous material during electron irradiation was published in 1947 by Watson who was then working in Canada. It was soon established that this carbonaceous material is formed from organic vapours, and it is now recognized that the principal source is the oil-sealed rotary pumps which provide the backing vacuum. It has been shown that the organic vapours consist of low molecular weight fragments of oil molecules which have been degraded at hot spots produced by friction between the vanes and the surfaces on which they slide. As satisfactory oil-free pumps are unavailable, it is standard electron microscope practice to reduce the partial pressure of organic vapours in the microscope in the vicinity of the specimen by using liquid-nitrogen cooled anti-contamination devices. Traps of this type are sufficient to reduce the contamination rate to about 0.1 Å per min, which is tolerable for many investigations.

Low-molecular-weight heparin in the prevention and treatment of thromboembolic disorders

1998 ◽  
Vol 1 (5) ◽  
pp. 166-174 ◽  
Author(s):  
Evelyn R Hermes De Santis ◽  
Betsy S Laumeister ◽  
Vidhu Bansal ◽  
Vandana Kataria ◽  
Preeti Loomba ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight Heparin ◽  
Low Molecular Weight ◽  
Prevention And Treatment
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