scholarly journals Investigation of the N-terminal coding region of MUC7 alterations in dentistry students with and without caries

2016 ◽  
Vol 19 (1) ◽  
pp. 71-76 ◽  
Author(s):  
L Koç Öztürk ◽  
A Yarat ◽  
S Akyuz ◽  
H Furuncuoglu ◽  
K Ulucan
Keyword(s):  
Molecular Weight ◽  
Oral Hygiene ◽  
Total Protein ◽  
Turkish Population ◽  
Dental Students ◽  
Oral Bacteria ◽  
World Health ◽  
Low Molecular Weight ◽  
Coding Region ◽  
Protein Levels

ABSTRACTHuman low-molecular weight salivary mucin (MUC7) is a small, secreted glycoprotein coded by MUC7. In the oral cavity, they inhibit the colonization of oral bacteria, including cariogenic ones, by masking their surface adhesions, thus helping saliva to avoid dental caries. The N-terminal domain is important for low-molecular weight (MG2) mucins to contact with oral microorganisms. In this study, we aimed to identify the N-terminal coding region of the MUC7 gene between individuals with and without caries. Forty-four healthy dental students were enrolled in this study; 24 of them were classified to have caries [decayed, missing, filled-teeth (DMFT) = 5.6] according to the World Health Organization (WHO) criteria, and 20 of them were caries-free (DMFT = 0). Simplified oral hygiene index (OHI-S) and gingival index (GI) were used to determine the oral hygiene and gingival conditions. Total protein levels and salivary total protein levels and salivary buffer capacity (SBC) were determined by Lowry and Ericsson methods. DNA was extracted from peripheral blood cells of all the participants and genotyping was carried out by a polymerase chain reaction (PCR)-sequencing method. No statistical differences were found between two groups in the terms of salivary parameters, oral hygiene and gingival conditions. We detected one common single nucleotide polymorphism (SNP) that leads to a change of asparagine to lysine at codon 80. This substitution was found in 29.0 and 40.0%, respectively, of the groups with and without caries. No other sequence variations were detected. The SNP found in this study may be a specific polymorphism affecting the Turkish population. Further studies with extended numbers are necessary in order to clarify this finding.

scholarly journals Protective Effect of Low Molecular Weight Peptides from Solenocera crassicornis Head against Cyclophosphamide-Induced Nephrotoxicity in Mice via the Keap1/Nrf2 Pathway

Antioxidants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 745
Author(s):  
Shuoqi Jiang ◽  
Zhuangwei Zhang ◽  
FangFang Huang ◽  
Zuisu Yang ◽  
Fangmiao Yu ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Target Genes ◽  
Inflammatory Responses ◽  
B Cell Lymphoma ◽  
Low Molecular Weight ◽  
Intragastric Administration ◽  
Related Factor ◽  
Downstream Target ◽  
Protein Levels ◽  
Total Antioxidant

The major component of the Solenocera crassicornis head protein hydrolysates-fraction 1 (SCHPs-F1) are low molecular weight peptides (MW < 1 kDa). In this study, we investigated the potential renoprotective effects of SCHPs-F1 in a cyclophosphamide (CTX) toxicity mouse model. In brief, 40 male mice were randomly divided into 5 groups and received either saline or 80 mg/kg body weight (BW) CTX by intraperitoneal injection for 5 days, followed by either saline or SCHPs-F1 (100, 200, and 400 mg/kg BW) by intragastric administration for 15 days. SCHPs-F1 treatment significantly reversed the CTX-induced decreases in the levels of blood urea nitrogen (BUN), creatinine (CRE), and cytochrome P450 (CYP450), as well as the renal histological lesions. Furthermore, the results indicated that SCHPs-F1 potentially alleviated CTX-induced nephrotoxicity through mitigating inflammatory responses, oxidative stress, and apoptosis status of the kidneys, as evidenced by decreased levels of malondialdehyde (MDA), interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ and increased levels of total antioxidant capacity (T-AOC), catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). Moreover, overexpression of pro-apoptotic proteins pair B-cell lymphoma-2 (Bcl-2)-associated X (Bax)/Bcl-2, cysteinyl aspartate specific proteinase (caspase)-3 and caspase-9 in renal tissues were suppressed by treatment with SCHPs-F1. In addition, the protein levels of the antioxidant factor nuclear factor erythroid-2 related factor 2 (Nrf2) and the expression levels of its downstream target genes heme-oxygenase (HO-1), glutamate-cysteine ligase modifier subunit (GCLM) and NAD(P)H dehydrogenase (quinone) 1 (NQO-1) were stimulated by treatment with SCHPs-F1 in the CTX-induced renal injury model. Taken together, our data suggested that SCHPs-F1 could provide a novel potential strategy in mitigating the nephrotoxicity caused by CTX.

scholarly journals The pathogenetic aspects of acute intestinal infections of suckling piglets

2020 ◽  
Vol 17 ◽  
pp. 00104
Author(s):  
Alexey G. Shakhov ◽  
Larisa Yu. Sashnina ◽  
Julia Y. Vladimirova ◽  
Maria Y. Jeines ◽  
Anatoly Y. Samuilenko
Keyword(s):  
Molecular Weight ◽  
Total Protein ◽  
Immune Status ◽  
Diagnostic Value ◽  
Low Molecular Weight ◽  
Functional Reserve ◽  
Complex Process ◽  
Intestinal Infections ◽  
Intestinal Pathology ◽  
Suckling Piglets

According to the studies piglets with intestinal infections compared to the clinically healthy animals, have lower the content of total protein, gamma globulins and general immunoglobulins, higher levels of malondialdehyde and endogenous intoxication, low molecular weight circulating immune complexes, lysozyme and complementary blood serum activity, phagocytic activity of neutrophils, their absorption and metabolic ability with a low functional reserve of phagocytes. The revealed changes in the biochemical, antioxidant, and immune status should be considered one of the pathogenetic mechanisms of the complex process of inflammation, as well as their diagnostic value in case of intestinal pathology caused by bacteria.

Quantifying Serum Derived Differential Expressed and Low Molecular Weight Protein in Breast Cancer Patients

2020 ◽  
Vol 27 (7) ◽  
pp. 658-673
Author(s):  
Ayesha Zafar ◽  
Maryum Jabbar ◽  
Yasmeen Manzoor ◽  
Huma Gulzar ◽  
Shahzad Gul Hassan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Molecular Weight ◽  
Total Protein ◽  
Early Stage ◽  
Low Molecular Weight ◽  
Molecular Weight Protein ◽  
Early Detection Of Disease ◽  
Differential Protein ◽  
Weight Protein ◽  
Low Molecular Weight Protein

Background: Searching the biomarker from complex heterogeneous material for early detection of disease is a challenging task in the field of biomedical sciences. Objective: The study has been arranged to explore the proteomics serum derived profiling of the differential expressed and low molecular weight protein in breast cancer patient. Methods: Quantitative proteome was analyzed using the Nano LC/Mass and Bioinformatics tool. Results: This quantification yields 239 total protein constituting 29% of differentially expressed protein, with 82% downregulated differential protein and 18% up-regulated differential protein. While 12% of total protein were found to be cancer inducing proteins. Gene Ontology (GO) described that the altered proteins with 0-60 kDa mass in nucleus, cytosol, ER, and mitochondria were abundant that chiefly controlled the RNA, DNA, ATP, Ca ion and receptor bindings. Conclusion: The study demonstrate that the organelle specific, low molecular weighted proteins are significantly important biomarker. That act as strong agents in the prognosis and diagnosis of breast cancer at early stage.

A review of the two major regulatory pathways for non-proprietary low-molecular-weight heparins

2012 ◽  
Vol 107 (02) ◽  
pp. 201-214 ◽  
Author(s):  
Frederick Ofosu
Keyword(s):  
Molecular Weight ◽  
Drug Application ◽  
World Health ◽  
European Medicines Agency ◽  
Low Molecular Weight ◽  
Regulatory Pathways ◽  
Synthetic Drugs ◽  
Chemical Structures ◽  
Asian Society ◽  
Health Organization

SummaryWith the expiry or pending expiry of originator low-molecular-weight heparin (LMWH) patents, pharmaceutical companies have invested in developing non-proprietary versions of LMWHs. LMWHs are manufactured by depolymerising highly purified unfractionated heparin. In contrast to traditional synthetic drugs with well-defined chemical structures, LMWHs contain complex oligosaccharide mixtures and the different manufacturing processes for LMWHs add to the heterogeneity in their physicochemical properties such that the European Medicines Agency (EMA) and the US Food and Drug Administration (FDA) consider existing originator LMWHs to be distinct medicinal entities that are not clinically interchangeable. The FDA views LMWHs as drugs and has approved two non-proprietary (generic) LMWHs, using the Abbreviated New Drug Application pathway. In contrast, the World Health Organization and the EMA view LMWHs as biological medicines. Therefore, the EMA and also the Scientific and Standardization Subcommittee on Anticoagulation of the International Society on Thrombosis and Haemostasis and the South Asian Society of Atherosclerosis and Thrombosis have all published specific guidelines for assessing non-proprietary (biosimilar) LMWHs. This manuscript reviews why there are two distinct pathways for approving non-proprietary LMWHs. Available literature on non-proprietary LMWHs approved in some jurisdictions is also reviewed in order to assess whether they satisfy the requirements for LMWHs in the three guidance documents. The review also highlights some of the significant difficulties the two pathways pose for manufacturers and an urgent need to develop a consensus governing the manufacture and regulation of non-proprietary LMWHs to make them more widely available.

scholarly journals Total protein, albumin and low-molecular-weight protein excretion in HIV-positive patients

2012 ◽  
Vol 13 (1) ◽  
Author(s):  
Lucy J Campbell ◽  
Tracy Dew ◽  
Rashim Salota ◽  
Emily Cheserem ◽  
Lisa Hamzah ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Total Protein ◽  
Hiv Positive ◽  
Low Molecular Weight ◽  
Molecular Weight Protein ◽  
Protein Excretion ◽  
Weight Protein ◽  
Low Molecular Weight Protein

scholarly journals The potential of low molecular weight heparin to mitigate cytokine storm in severe COVID-19 patients: a retrospective clinical study

2020 ◽  
Author(s):  
Chen Shi ◽  
Cong Wang ◽  
Hanxiang Wang ◽  
Chao Yang ◽  
Fei Cai ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight Heparin ◽  
Complete Blood Count ◽  
World Health ◽  
Therapeutic Drug ◽  
Control Group ◽  
Controlled Clinical Trial ◽  
Low Molecular Weight ◽  
Retrospective Clinical Study ◽  
Before And After

SummaryBackgroundOn March 11, 2020, the World Health Organization declared its assessment of COVID-19 as a global pandemic. However, specific antiviral drugs are still unavailable, and pateints are managed by multiple complementary treatments.MethodsThe electronic medical records of COVID-19 patients where basic information, complete blood count, coagulation profile, inflammatory cytokines and serum biochemical indicators in 42 patients with COVID-19 (21 of whom were treated with low molecular weight heparin (LMWH), and 21 without LMWH) that were retrospectively analyzed to compare and evaluate the effect of LMWH treatment on disease progression.Findings42 patients with COVID-19 treated at the hospital between February 1 and March 15, 2020, were selected for the study, of which 21 underwent LMWH treatment (LMWH group), and 21 did not (Control), during hospitalization. Changes in the percentage of lymphocytes in the LMWH group before and after LMWH treatment were significantly different from those in the control group (11·10±9·50 vs. 3·08±9·66, p=0·011, respectively). Changes in the levels of D-dimer and fibrinogen degradation products (FDP) in the LMWH group before and after LMWH treatment were significantly different from those in the control group (-2·85±3·90, -0·05±0.85, p=0·002; -9·05±13·14, -1·78±3·15, p=0·035). Strikingly, in the LMWH group, IL-6 levels were significantly reduced after LMWH treatment (47·47±58·86, 15·76±25·71, p=0·006). Besides, the changes in IL-6 levels in the LMWH group before and after LMWH treatment were significantly different from those in the control group (-32·46±65·97, 14·96±151·09, p=0·031).InterpretationLMWH improves the coagulation dysfunction of COVID-19 patients and exerts anti-inflammatory effects by reducing IL-6 and increasing lymphocyte %. It appears that LMWH can be used as a potential therapeutic drug for the treatment of COVID-19, paving the way for a subsequent well-controlled clinical trial.FundingNational Natural Science Foundation of China (No. 81603037 to SC) and the National Key Research and Development Plan of China(2017YFC0909900).

scholarly journals Proteinuria in rats in relation to age-dependent renal changes

1980 ◽  
Vol 14 (2) ◽  
pp. 95-101 ◽  
Author(s):  
Jeannette M. Alt ◽  
H. Hackbarth ◽  
F. Deerberg ◽  
H. Stolte
Keyword(s):  
Molecular Weight ◽  
Total Protein ◽  
Plasma Proteins ◽  
Disc Electrophoresis ◽  
Male Rats ◽  
Low Molecular Weight ◽  
Urinary Proteins ◽  
Protein Excretion ◽  
Age Dependent ◽  
Renal Changes

A variety of sex-dependent urinary proteins of low molecular weight, absent in females and in castrated males, can be identified in male rats by disc electrophoresis. In the urine of male rats of age 5·5 months, albumin comprises only 1-2% of the total protein. Albumin excretion increases greatly with age and associated kidney disease. Total protein excretion, however, stays the same or even decreases slightly as the rat ages, due to a loss of low molecular weight, sex-dependent, proteins. These are virtually absent in senescent rats (38 months of age), although total protein excretion rises tenfold in these animals due to high molecular weight plasma proteins passing into the urine; the glomerular filtration rate decreases to 70% of the value measured at 5·5 months of age.

Sign in / Sign up

Export Citation Format

Share Document