scholarly journals Vectors for the development of high-tech industrial aquaculture

2020 ◽  
Vol 27 ◽  
pp. 00132
Author(s):  
E. M. Romanova ◽  
V. V. Romanov ◽  
V. N. Lyubomirova ◽  
L. A. Shadieva ◽  
T. M. Shlenkina

The article discusses the prospects of optimizing the technology of growing African catfish in high-tech industrial aquaculture. The goal was to determine the range of biologically active substances that can increase the productivity, organoleptic qualities of fish grown in recirculating aquaculture systems and optimize their living conditions. For this purpose, a complex of biologically active substances was tested, including probiotics, prebiotics, amino acids, and natural sorbents. It was introduced into feed and added to the water of fish tanks. Studies have shown that the developed complex of biologically active substances accelerates growth and development, guarantees the growth of fish biomass, reduces the level of bacterial pollution of the fish habitat, and normalizes hydrochemical parameters in the tanks. Improving the technology of fish farming in recirculating aquaculture systems (RAS) involves changing the ideology of feeding and keeping fish. The results of our studies show that, under conditions of artificial breeding in RAS, it is necessary to use cultures of living microorganisms probiotics that improve intestinal microbiocenosis in fish feeding and introduce them into the fish habitat. Probiotics should be used with prebiotics containing vitamin-amino acid complexes that stimulate the development of symbiotic microbiota. The use of natural sorbents plays an important role. The proposed set of ingredients increases the organoleptic qualities of fish. The studies were supported by the Russian Foundation for Basic Research with grant 18-016-00127.

2021 ◽  
Vol 247 ◽  
pp. 01055
Author(s):  
G.H. Gumerova ◽  
M.R. Vakhitov ◽  
I.S. Dokuchaeva

The foundations of efficient production are laid at the stage of developing a high-tech product. The object of research is wild berries (lingonberry, honeysuckle, viburnum, cranberry, cloudberry, sea buckthorn, mountain ash, aronia), which are used as a source of a natural complex of biologically active substances and trace elements as the main substance of a new parapharmaceutical. The primary technological task of food engineering of paramarceuticals is the maximum extraction of biologically active substances of plant raw materials and their preservation in their native form during the warranty period of storage. Therefore, as the main criterion for changing the properties of raw materials during processing, we used the amount of change in the redox potential of the system. A set of methodological approaches has been developed to ensure the quality and safety of the new parapharmaceutical.


2020 ◽  
Vol 62 (1-2) ◽  
pp. 49-68
Author(s):  
T. O. Kondratiuk ◽  
T. V. Beregova ◽  
I. Yu. Parnikoza ◽  
S. Y. Kondratyuk ◽  
A. Thell

The identification of the diversity of microscopic fungi of lithobiont communities of the Argentine Islands in specimens collected during the 22nd Ukrainian Antarctic Expedition was the purpose of this work. Samples of rock, soil, mosses and lichens of rock micro-habitats of “Crustose lichen sub-formation and fruticose lichen and moss cushion sub-formation” were used in the work. These samples were used for extracting and cultivation of filamentous fungi on dense nutrient media. Determination of physiological and biochemical characteristics and identification of yeast-like fungi were performed using a microbiological analyser ‘Vitek-2’ (‘Bio Merieux’, France). Cultivation of microorganisms was carried out at temperatures from +2 to +37 °C. In results cultures of microscopic fungi of Zygomycota (Mucor circinelloides), Ascomycota (species of the genera cf. Tlielebolus, Talaromyces), representatives of the Anamorphic fungi group (Geomyces pannorum, species of the genera Alternaria, Acremonium, Aspergillus, Penicillium, and Cladosporium) were isolated from Antarctic samples. Microscopic fungi Penicillium spp. were dominated after the frequency in the studied samples (54.5%). Rhodotorula rubra and Candida sp. among isolated yeast fungi, and dark pigmented fungi represented by Aureobasidium pulhdans and Exophiala spp. were identified. The biological properties of a number of isolated fungi (the potential ability to synthesise important biologically active substances: melanins, carotenoids, lipids) are characterised. Mycobiota of rock communities of Argentine Islands is rich on filamentous and yeast fungi similarly to other regions of Antarctica. A number of fungi investigated are potentially able to synthesise biologically active substances. The dark pigmented species of the genera Cladosporium, Exophiala, Aureobasidium pulhdans, capable of melanin synthesis; ‘red’ yeast Rhodotorula rubra (carotenoid producers and resistant to toxic metals); Mucor circinelloides and Geomyces pannorum, lipid producers, are among these fungi. Yeast-like fungi assimilated a wide range of carbohydrates, which will allow them to be further used for cultivation in laboratory and process conditions. The collection of technologically promising strains of microorganisms, part of the Culture Collection of Fungi at Taras Shevchenko National University of Kyiv (Ukraine), is updated with isolated species (strains) of filamentous fungi and yeast – potential producers of biologically active substances, obtained within this study.


Biomics ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 389-393
Author(s):  
D.V. Mitrofanov ◽  
N.V. Budnikova

The drone brood contains a large number of substances with antioxidant activity. These substances require stabilization and strict adherence to storage conditions. Among these substances are unique decenoic acids, the content of which is an indicator of the quality of drone brood and products based on it. The ability of drone brood to reduce the manifestations of oxidative stress is shown. There are dietary supplements for food and drugs based on drone brood, which are used for a wide range of diseases. Together with drone brood, chitosan-containing products, propolis, royal jelly can be used. They enrich the composition with their own biologically active substances and affect the preservation of the biologically active substances of the drone brood. Promising are the products containing, in addition to the drone brood, a chitin-chitosan-melanin complex from bees, propolis, royal jelly. The chitin-chitosan-melanin complex in the amount of 5% in the composition of the adsorbent practically does not affect the preservation of decenic acids, while in the amount of 2% and 10% it somewhat worsens. The acid-soluble and water-soluble chitosan of marine crustaceans significantly worsens the preservation of decenoic acids in the product. Drone brood with royal jelly demonstrates a rather high content of decenoic acids. When propolis is introduced into the composition of the product, the content of decenoic acids increases according to the content of propolis.


2018 ◽  
Vol 22 (2) ◽  
pp. 263-266
Author(s):  
R.V. Kutsyk ◽  
O.I. Yurchyshyn

The emergence of microorganisms resistant strains is a natural biological response to the use of antimicrobial drugs that creates selective pressure, contributing to pathogens selection, survival and reproduction. The purpose of the investigation was to study the resistance development of staphylococci skin isolates to erythromycin and influence on it Alnus incana L. fruit extract subinhibitory concentrations. Development of resistance to erythromycin and influence on it Alnus incana L. fruit extract (extraction by 90% ethanol) subinhibitory concentrations were conducted with S epidermidis strains: sensitive and resistant to 14 and 15-membered macrolides. The study was carried out within 30 days by multiple consecutive passages of staphylococci test strains (concentration 1×107 CFU/ml) into test tubes containing broth and erythromycin ranging from 3 doubling dilutions above to doubling dilutions below the minimum inhibitory concentration. Statistical analysis of the results was carried out by one-and two-factor analysis of variance (ANOVA) and Microsoft Office Excel 2011. Rapid increase of resistance from 32 to 1024 μg/ml (F=34.2804; F> Fstand. max = 5.9874; p=0.0011) for S.epidermidis with a low level of resistance to 14 and 15-membered macrolides resistance to the erythromycine was observed. In the presence of Alnus incana L. fruit extract subinhibitory concentrations (¼ MIC), the initial MIC of erythromycin was decreased by 32 times to 1 μg/ml (F = 9.7497; F> Fstand. max = 5.9874; p = 0.0205). The sensitive strain after 30 passages did not develop resistance to erythromycin. Under the influence of erythromycin selective pressure, S.epidermidis strain with low initial level of MLS-resistance rapidly reaches a high-level resistance. Biologically active substances of the Alnus incana L. fruit extract significantly inhibit the resistance development in S. epidermidis to macrolides and eliminate it phenotypic features.


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