scholarly journals Antioxidant Activities of Centella asiatica Extract-loaded Bovine Serum Albumin Nanoparticles in Simulated Gastrointestinal System Study

2020 ◽  
Vol 141 ◽  
pp. 03001
Author(s):  
Kittiya Kesornbuakao ◽  
Patteera Chanapongpisan ◽  
Malinee Sriariyanun ◽  
Ir. Lindayani ◽  
Patchanee Yasurin
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Crude Extract ◽  
Bovine Serum ◽  
Antioxidant Activities ◽  
Centella Asiatica ◽  
Gastrointestinal System ◽  
Physical Parameters ◽  
Active Compounds ◽  
Albumin Nanoparticles

Bioavailability of active compounds extracted from herbs is generally limited to be adsorbed or expressed to target organisms due to several physical and chemical factors. Nanoparticle encapsulation techniques was developed to carry bioactive macromolecules of Centella asiatica (Buabok) in the form of C. asiatica Extract-loaded Bovine Serum Albumin Nanoparticles (CBNPs) to improve bioavailability. In this study, the antioxidant activities of CBNPs and C. asiatica crude extract were evaluated by using DPPH radical scavenging assay in the simulated gastrointestinal system, including mastication, stomach, duodenum, and ileum conditions to provide the environment which similar to in vivo system in terms of chemical and physical parameters. CBNPs were prepared by mixing of C. asiatica crude extract and BSA at different ratio of 1:2, 1:3, and 1:4. The results showed that the highest antioxidant activity of CBNP was observed when the ratio of crude extract and BSA at 1:2 was used at pH 2.0 or in the simulated stomach condition. The denaturation or unfolding of BSA in the simulated stomach occurred when pH was low could lead to the release of active compounds at certain area in gastrointestinal system.

Transfecting pDNA to E . coli DH5α using bovine serum albumin nanoparticles as a delivery vehicle

Luminescence ◽  
2014 ◽  
Vol 30 (5) ◽  
pp. 583-591 ◽  
Author(s):  
Jitendra Wagh ◽  
Kuldeep J. Patel ◽  
Parth Soni ◽  
Krutika Desai ◽  
Pratik Upadhyay ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Delivery Vehicle ◽  
E Coli ◽  
Albumin Nanoparticles

Quality by Design Approach for Development and Optimization of Rifampicin Loaded Bovine Serum Albumin Nanoparticles and Characterization

2021 ◽  
Vol 18 ◽  
Author(s):  
Monica Joshi ◽  
Khushwant S. Yadav ◽  
Bala Prabhakar
Keyword(s):  
Particle Size ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Quality By Design ◽  
Entrapment Efficiency ◽  
Quality Attributes ◽  
Albumin Nanoparticles ◽  
Therapeutic Benefits ◽  
Intravenous Application

Background: Rifampicin is one of the first line drugs used for tuberculosis therapy. The therapy lasts for a long time. Thus, there is a need to develop sustained release formulation of rifampicin for intravenous application. Aim: This study is focused on preparing rifampicin loaded bovine serum albumin nanoparticles (RIF BSA NPs) suitable for intravenous application using systematic quality by design (QbD) approach. Objectives: The main objective of this study is optimizing particle size and entrapment efficiency of rifampicin loaded bovine serum albumin nanoparticles (RIF BSA NPs) and making it suitable for intravenous application using QbD approach. Methods: Quality target product profile was defined along with critical quality attributes (CQAs) for the formulation. 32 factorial design was used for achieving the predetermined values of CQAs, i.e., mean particle size <200 nm and percent entrapment efficiency>50%. Incubation time of drug with colloidal albumin solution and ratio of rifampicin: albumin, were selected as independent variables. Check point analysis was performed to confirm the suitability of regression model for optimization. Results: : The optimized RIF BSA NPs were characterized by FTIR, DSC, 1H NMR techniques. The NPs observed by transmission electron microscopy were spherical in shape. The rifampicin release could be sustained for 72 hours from BSA NPs matrix. RIF BSA NPs dispersion was stable at 5 ± 3°C for 72 hours. Non-toxicity of nanoparticles to RAW 264.7 cell line was proved by MTT assay. Conclusion: Development of RIF BSA NPs with desired quality attributes was possible by implementing QbD approach. The optimized formulation suitable for intravenous application can potentially improve the therapeutic benefits of rifampicin.

scholarly journals Improved drug targeting to liver tumor by sorafenib-loaded folate-decorated bovine serum albumin nanoparticles

Drug Delivery ◽  
2019 ◽  
Vol 26 (1) ◽  
pp. 89-97 ◽  
Author(s):  
Haipeng Wang ◽  
Shuilin Sun ◽  
Yu Zhang ◽  
Jiayi Wang ◽  
Shouhua Zhang ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Liver Tumor ◽  
Bovine Serum ◽  
Drug Targeting ◽  
Albumin Nanoparticles

Biotin-modified bovine serum albumin nanoparticles as a potential drug delivery system for paclitaxel

2019 ◽  
Vol 54 (11) ◽  
pp. 8613-8626 ◽  
Author(s):  
Danfeng Wang ◽  
Na Liang ◽  
Yoshiaki Kawashima ◽  
Fude Cui ◽  
Pengfei Yan ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Drug Delivery System ◽  
Delivery System ◽  
Bovine Serum ◽  
Potential Drug ◽  
Albumin Nanoparticles

Presentation of a nano‐based tag for immunoassay, based on amine‐modified bovine serum albumin nanoparticles

2015 ◽  
Vol 9 (1) ◽  
pp. 43-51 ◽  
Author(s):  
Ali Jebali ◽  
Seyedhossein Hekmatimoghaddam ◽  
Bahram Kazemi ◽  
Jesus Martinez De La Fuente
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Albumin Nanoparticles
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