Nanometric plasmonic optical trapping on gold nanostructures

2019 ◽  
Vol 86 (3) ◽  
pp. 30501
Author(s):  
Domna G. Kotsifaki ◽  
Mersini Makropoulou ◽  
Alexander A. Searfetinides
Keyword(s):  
Optical Tweezers ◽  
Single Molecule ◽  
Optical Trapping ◽  
Numerical Aperture ◽  
Resonance Wavelength ◽  
Objective Lens ◽  
Plasmonic Nanostructures ◽  
Theoretical Support ◽  
Trapping Force ◽  
Trapping Forces

The precise noninvasive optical manipulation of nanometer-sized particles by evanescent fields, instead of the conventional optical tweezers, has recently awaken an increasing interest, opening a way for investigating phenomena relevant to both fundamental and applied science. In this work, the optical trapping force exerted on trapped dielectric nanoparticle was theoretically investigated as a function on the trapping beam wavelength and as a function of several plasmonic nanostructures schemes based on numerical simulation. The maximum optical trapping forces are obtained at the resonance wavelength for each plasmonic nanostructure geometry. Prominent tunabilities, such as radius and separation of gold nanoparticles as well as the numerical aperture of objective lens were examined. This work will provide theoretical support for developing new types of plasmonic sensing substrates for exciting biomedical applications such as single-molecule fluorescence.

Axial optical trapping forces on two particles trapped simultaneously by optical tweezers

2005 ◽  
Vol 44 (13) ◽  
pp. 2667 ◽  
Author(s):  
Shenghua Xu ◽  
Yinmei Li ◽  
Liren Lou
Keyword(s):  
Optical Tweezers ◽  
Optical Trapping ◽  
Trapping Forces

Nanowire-type plasmonic waveguides as strong and tunable optical tweezers

2019 ◽  
Vol 33 (07) ◽  
pp. 1950081 ◽  
Author(s):  
Shu Yang ◽  
Kang Zhao
Keyword(s):  
Long Range ◽  
Optical Tweezers ◽  
Optical Trapping ◽  
Near Field ◽  
Optical Forces ◽  
Plasmonic Waveguides ◽  
Trapping Forces

A series of nanowire-type plasmonic waveguides are proposed. The mode properties of these waveguides and their dependences on various geometry parameters are studied. It is shown that they can generate deep subwavelength confinement and long-range propagation simultaneously. Moreover, the optical forces exerted on dielectric nanoparticles by these waveguides are calculated. It is found that the optical trapping forces are very strong, and that their distribution can be effectively regulated by certain geometry parameters. Using these features, strong and tunable near-field optical tweezers can be designed.

Optical trapping with low numerical aperture objective lens

2012 ◽  
Author(s):  
R. Dasgupta ◽  
S. Ahlawat ◽  
P. K. Gupta ◽  
J. Xavier ◽  
J. Joseph
Keyword(s):  
Optical Trapping ◽  
Numerical Aperture ◽  
Objective Lens

scholarly journals Three-Dimensional Optical Trapping of a Plasmonic Nanoparticle using Low Numerical Aperture Optical Tweezers

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Oto Brzobohatý ◽  
Martin Šiler ◽  
Jan Trojek ◽  
Lukáš Chvátal ◽  
Vítězslav Karásek ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Optical Trapping ◽  
Three Dimensional ◽  
Numerical Aperture ◽  
Plasmonic Nanoparticle

scholarly journals High-numerical-aperture cryogenic light microscopy for increased precision of superresolution reconstructions

2017 ◽  
Vol 114 (15) ◽  
pp. 3832-3836 ◽  
Author(s):  
Marc Nahmani ◽  
Conor Lanahan ◽  
David DeRosier ◽  
Gina G. Turrigiano
Keyword(s):  
Single Molecule ◽  
Room Temperature ◽  
Fluorescent Protein ◽  
Numerical Aperture ◽  
Fluorescent Imaging ◽  
Objective Lens ◽  
High Numerical Aperture ◽  
Superresolution Microscopy ◽  
Superresolution Imaging ◽  
Photoactivated Localization Microscopy

Superresolution microscopy has fundamentally altered our ability to resolve subcellular proteins, but improving on these techniques to study dense structures composed of single-molecule-sized elements has been a challenge. One possible approach to enhance superresolution precision is to use cryogenic fluorescent imaging, reported to reduce fluorescent protein bleaching rates, thereby increasing the precision of superresolution imaging. Here, we describe an approach to cryogenic photoactivated localization microscopy (cPALM) that permits the use of a room-temperature high-numerical-aperture objective lens to image frozen samples in their native state. We find that cPALM increases photon yields and show that this approach can be used to enhance the effective resolution of two photoactivatable/switchable fluorophore-labeled structures in the same frozen sample. This higher resolution, two-color extension of the cPALM technique will expand the accessibility of this approach to a range of laboratories interested in more precise reconstructions of complex subcellular targets.

scholarly journals Robust optical autofocus system utilizing neural networks trained for extended range and time-course and automated multiwell plate imaging including single molecule localization microscopy

2021 ◽  
Author(s):  
Jonathan Lightley ◽  
Frederik Görlitz ◽  
Sunil Kumar ◽  
Ranjan Kalita ◽  
Arinbjorn Kolbeinsson ◽  
...  
Keyword(s):  
Data Acquisition ◽  
Single Molecule ◽  
Time Course ◽  
Numerical Aperture ◽  
Image Data ◽  
Training Data ◽  
Depth Of Field ◽  
Objective Lens ◽  
Localisation Microscopy ◽  
The Impact

We present a robust, long-range optical autofocus system for microscopy utilizing machine learning. This can be useful for experiments with long image data acquisition times that may be impacted by defocusing resulting from drift of components, e.g. due to changes in temperature or mechanical drift. It is also useful for automated slide scanning or multiwell plate imaging where the sample(s) to be imaged may not be in the same horizontal plane throughout the image data acquisition. To address the impact of (thermal or mechanical) fluctuations over time in the optical autofocus system itself, we utilise a convolutional neural network (CNN) that is trained over multiple days to account for such fluctuations. To address the trade-off between axial precision and range of the autofocus, we implement orthogonal optical readouts with separate CNN training data, thereby achieving an accuracy well within the 600 nm depth of field of our 1.3 numerical aperture objective lens over a defocus range of up to approximately +/-100 μm. We characterise the performance of this autofocus system and demonstrate its application to automated multiwell plate single molecule localisation microscopy.

scholarly journals Resonator nanophotonic standing-wave array trap for single-molecule manipulation and measurement

2022 ◽  
Vol 13 (1) ◽  
Author(s):  
Fan Ye ◽  
James T. Inman ◽  
Yifeng Hong ◽  
Porter M. Hall ◽  
Michelle D. Wang
Keyword(s):  
Standing Wave ◽  
Single Molecule ◽  
Biological Molecules ◽  
Force Generation ◽  
Dna Molecules ◽  
Force Extension ◽  
Resonator Design ◽  
Trapping Force ◽  
On Chip ◽  
Trapping Forces

AbstractNanophotonic tweezers represent emerging platforms with significant potential for parallel manipulation and measurements of single biological molecules on-chip. However, trapping force generation represents a substantial obstacle for their broader utility. Here, we present a resonator nanophotonic standing-wave array trap (resonator-nSWAT) that demonstrates significant force enhancement. This platform integrates a critically-coupled resonator design to the nSWAT and incorporates a novel trap reset scheme. The nSWAT can now perform standard single-molecule experiments, including stretching DNA molecules to measure their force-extension relations, unzipping DNA molecules, and disrupting and mapping protein-DNA interactions. These experiments have realized trapping forces on the order of 20 pN while demonstrating base-pair resolution with measurements performed on multiple molecules in parallel. Thus, the resonator-nSWAT platform now meets the benchmarks of a table-top precision optical trapping instrument in terms of force generation and resolution. This represents the first demonstration of a nanophotonic platform for such single-molecule experiments.

scholarly journals Optical tweezers in single-molecule experiments

2020 ◽  
Vol 135 (11) ◽  
Author(s):  
Annamaria Zaltron ◽  
Michele Merano ◽  
Giampaolo Mistura ◽  
Cinzia Sada ◽  
Flavio Seno
Keyword(s):  
Optical Tweezers ◽  
Single Molecule ◽  
Optical Trapping ◽  
Biological Systems ◽  
Force Spectroscopy ◽  
Research Field ◽  
Single Molecule Force Spectroscopy ◽  
Control Forces ◽  
And Control ◽  
Physical Principles

Abstract In the last decades, optical tweezers have progressively emerged as a unique tool to investigate the biophysical world, allowing to manipulate and control forces and movements of one molecule at a time with unprecedented resolution. In this review, we present the use of optical tweezers to perform single-molecule force spectroscopy investigations from an experimental perspective. After a comparison with other single-molecule force spectroscopy techniques, we illustrate at an introductory level the physical principles underlying optical trapping and the main experimental configurations employed nowadays in single-molecule experiments. We conclude with a brief summary of some remarkable results achieved with this approach in different biological systems, with the aim to highlight the great variety of experimental possibilities offered by optical tweezers to scientists interested in this research field.

scholarly journals Experimental investigation on optical vortex tweezers for microbubble trapping

Open Physics ◽  
2018 ◽  
Vol 16 (1) ◽  
pp. 383-386 ◽  
Author(s):  
Xiaoming Zhou ◽  
Ziyang Chen ◽  
Zetian Liu ◽  
Jixiong Pu
Keyword(s):  
Optical Tweezers ◽  
Laser Power ◽  
Topological Charge ◽  
Force Measurement ◽  
Numerical Aperture ◽  
Optical Vortex ◽  
Gradient Force ◽  
Vortex Beam ◽  
Trapping Forces ◽  
Topological Charges

AbstractIn this paper, we investigated the microbubble trapping using optical vortex tweezers. It is shown that the microbubble can be trapped by the vortex optical tweezers, in which the trapping light beam is of vortex beam. We studied a relationship between the transverse capture gradient force and the topological charges of the illuminating vortex beam. For objective lenses with numerical aperture of 1.25 (100×), the force measurement was performed by the power spectral density (PSD) roll-off method. It was shown that transverse trapping forces of vortex optical tweezers increase with the increment of the laser power for fixed topological charge. Whereas, the increase in the topological charges of vortex beam for the same laser power results in the decrease of the transverse trapping forces. The experimental results demonstrated that the laser mode (topological charge) has significant influence on the lateral trapping force.

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