scholarly journals Separation and identification of polar polyphenols in oily formulation using high-performance thin-layer chromatography and mass spectroscopy techniques

OCL ◽  
2018 ◽  
Vol 25 (5) ◽  
pp. D506 ◽  
Author(s):  
Ophélie Fadel ◽  
Donatien Gomes Rodrigues ◽  
Luc Girard ◽  
Pierre Bauduin ◽  
Anne Rossignol-Castera ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Vegetable Oil ◽  
Mass Spectroscopy ◽  
High Performance ◽  
Extraction Process ◽  
Oil Formulation ◽  
Layer Chromatography

In cosmetics, there is some interest to solubilized polar antioxidant molecules in oil. This is a matrix that can indeed protect these fragile molecules against oxidation and that makes possible the polar antioxidants transfer through the epidermis in order to be delivered within the dermis. Varying the oil formulation allows the achievement of a high content of antioxidants in a natural vegetable oil using an oleo-eco extraction process. However, it was necessary to characterize this content using quantitative and efficient methods such as High-Performance Thin-Layer Chromatography technique. The aim of this work was to develop a method of antioxidant’s characterization with this technique without a preliminary re-extraction of the antioxidants.

scholarly journals Recovery of Bioactive Compounds from Hazelnuts and Walnuts Shells: Quantitative–Qualitative Analysis and Chromatographic Purification

Biomolecules ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1363 ◽  
Author(s):  
René Herrera ◽  
Jarl Hemming ◽  
Annika Smeds ◽  
Oihana Gordobil ◽  
Stefan Willför ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Thin Layer ◽  
Antioxidant Capacity ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Short Chain Fatty Acids ◽  
Extraction Process ◽  
Size Exclusion ◽  
Flash Chromatography ◽  
Layer Chromatography

Hazelnut (HS) and walnut (WS) shells, an abundant by-product of the processing industries of these edible nuts, are traditionally considered as a low-value waste. However, they are a source of valuable compounds with an interesting chemical profile for the chemical and pharmaceutical sectors. In this study, the lipophilic and hydrophilic extracts present in HS and WS were quantified and identified, then the polar fractions were chromatographically separated, and their antioxidant capacity was studied. The experimental work includes the isolation of crude lipophilic and hydrophilic extracts by an accelerated extraction process, chromatographic analysis (gas chromatography-flame ionization (GC-FID), GC-mass spectroscopy (GC-MS), high-performance size-exclusion chromatography (HPSEC), thin-layer chromatography (TLC)), and quantification of the components. In addition, a thorough compositional characterization of the subgroups obtained by flash chromatography and their antioxidant capacity was carried out. The gravimetric concentrations showed different lipophilic/hydrophilic ratios (0.70 for HS and 0.23 for WS), indicating a higher proportion of polar compounds in WS than in HS. Moreover, the lipophilic extracts were principally composed of short-chain fatty acids (stearic, palmitic, and oleic acid), triglycerides, and sterols. The polar fractions were screened by thin-layer chromatography and then separated by flash chromatography, obtaining fractions free of fatty acids and sugar derivatives (97:3 in HS and 95:5 in WS), and mixtures richer in phenolic compounds and flavonoids such as guaiacyl derivatives, quercetin, pinobanksin, and catechin. The most polar fractions presented a higher antioxidant capacity than that of the crude extracts.

scholarly journals Isolation and Structure Elucidation of Quercetin like Structure from Dalbergia sissoo (Fabaceae)

2020 ◽  
Vol 10 (3-s) ◽  
pp. 6-11
Author(s):  
Rohit Kumar KUMAR Bijauliya ◽  
Pushpendra Kannojia ◽  
Pankaj Mishra ◽  
Gaurav Kumar Pathak
Keyword(s):  
Nmr Spectroscopy ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mass Spectroscopy ◽  
Column Chromatography ◽  
Structure Elucidation ◽  
High Performance ◽  
18Th Century ◽  
Dalbergia Sissoo ◽  
Layer Chromatography

The present study was conducted to isolate and classify Dalbergia sissoo (L.) bioactive compounds. The genus consists of 300 species in India, including 25 species. The generic name Dalbergia honors the 18th century Swedish brothers Nils and Carl Dalberg. Various phytoconstituents of alkaloids, glycosides, flavanoids, tannins, saponins, sterols and terpenoids were isolated and classified from different parts of the plant. Thin Layer Chromatography, High Performance Thin Layer Chromatography, and Column Chromatography were used to isolate spots from the fraction of the crude extract to elucidate the chemical structure of Dalbergia sissoo (L.) leaf extract. Potential spots have been exposed to techniques of FTIR, NMR and mass spectroscopy. Column chromatography was exposed to the raw extract, obtaining 125 fractions, conducting TLC. Among them was a single band in TLC, characterized by FTIR, NMR spectroscopy and mass spectroscopy, and the structure was known as Quercetin. The results of this study indicate the effective potential compound of the ethanolic fraction of Dalbergia sissoo (L.). Keywords: Dalbergia sissoo; Isolation & Structure Elucidation; FTIR; NMR spectroscopy; Mass spectroscopy.

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