Th1/Th17 Immune Response Is Induced by Mesenteric Lymph Node Dendritic Cells in Crohn's Disease

2009 ◽  
Vol 137 (5) ◽  
pp. 1736-1745 ◽  
Author(s):  
Atsushi Sakuraba ◽  
Toshiro Sato ◽  
Nobuhiko Kamada ◽  
Mina Kitazume ◽  
Akira Sugita ◽  
...  
Keyword(s):  
Immune Response ◽  
Dendritic Cells ◽  
Crohn’S Disease ◽  
Lymph Node ◽  
Crohn's Disease ◽  
Mesenteric Lymph Node ◽  
Mesenteric Lymph

scholarly journals The Human Mesenteric Lymph Node Microbiome Differentiates Between Crohn’s Disease and Ulcerative Colitis

2018 ◽  
Vol 13 (1) ◽  
pp. 58-66 ◽  
Author(s):  
Miranda G Kiernan ◽  
J Calvin Coffey ◽  
Kieran McDermott ◽  
Paul D Cotter ◽  
Raul Cabrera-Rubio ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Crohn’S Disease ◽  
Lymph Node ◽  
Crohn's Disease ◽  
Mesenteric Lymph Node ◽  
Mesenteric Lymph

scholarly journals Protective Mucosal Th2 Immune Response against Toxoplasma gondii by Murine Mesenteric Lymph Node Dendritic Cells

2003 ◽  
Vol 71 (9) ◽  
pp. 5254-5265 ◽  
Author(s):  
Isabelle Dimier-Poisson ◽  
Fleur Aline ◽  
Marie-Noëlle Mévélec ◽  
Céline Beauvillain ◽  
Dominique Buzoni-Gatel ◽  
...  
Keyword(s):  
Immune Response ◽  
Dendritic Cells ◽  
Lymph Node ◽  
Toxoplasma Gondii ◽  
Mesenteric Lymph Node ◽  
Ex Vivo ◽  
The Body ◽  
Mouse Strains ◽  
Mesenteric Lymph ◽  
Th2 Immune Response

ABSTRACT Toxoplasma gondii, an obligate intracellular parasite pathogen which initially invades the intestinal epithelium before disseminating throughout the body, may cause severe sequelae in fetuses and life-threatening neuropathy in immunocompromised patients. Immune protection is usually thought to be performed through a systemic Th1 response; considering the route of parasite entry it is important to study and characterize the local mucosal immune response to T. gondii. Despite considerable effort, Toxoplasma-targeted vaccines have proven to be elusive using conventional strategies. We report the use of mesenteric lymph node dendritic cells (MLNDCs) pulsed ex vivo with T. gondii antigens (TAg) as a novel investigation approach to vaccination against T. gondii-driven pathogenic processes. Using a murine model, we demonstrate in two genetically distinct mouse strains (C57BL/6 and CBA/J) that adoptively transferred TAg-pulsed MLNDCs elicit a mucosal Toxoplasma-specific Th2-biased immune response in vivo and confer strong protection against infection. We also observe that MLNDCs mostly traffic to the intestine where they enhance resistance by reduction in the mortality and in the number of brain cysts. Thus, ex vivo TAg-pulsed MLNDCs represent a powerful tool for the study of protective immunity to T. gondii, delivered through its natural route of entry. These findings might impact the design of vaccine strategies against other invasive microorganisms known to be delivered through digestive tract.

scholarly journals Peripheral blood and mesenteric lymph node lymphocytes in Crohn's disease.

Gut ◽  
1980 ◽  
Vol 21 (6) ◽  
pp. 507-511 ◽  
Author(s):  
E R Richens ◽  
C M Thorp ◽  
P W Bland ◽  
K R Gough
Keyword(s):  
Crohn’S Disease ◽  
Lymph Node ◽  
Crohn's Disease ◽  
Mesenteric Lymph Node ◽  
Peripheral Blood ◽  
Mesenteric Lymph

scholarly journals IFN-γ Secreted by CD103+Dendritic Cells Leads to IgG Generation in the Mesenteric Lymph Node in the Absence of Vitamin A

2011 ◽  
Vol 186 (12) ◽  
pp. 6999-7005 ◽  
Author(s):  
Jae-Hoon Chang ◽  
Hye-Ran Cha ◽  
Sun-Young Chang ◽  
Hyun-Jeong Ko ◽  
Sang-Uk Seo ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Lymph Node ◽  
Vitamin A ◽  
Mesenteric Lymph Node ◽  
Mesenteric Lymph ◽  
Ifn Γ

scholarly journals Immunofluorescent characterization of non-myelinating Schwann cells and their interactions with immune cells in mouse mesenteric lymph node

2017 ◽  
Vol 61 (3) ◽  
Author(s):  
Zhongli Shi ◽  
Wayne K. Greene ◽  
Philip K. Nicholls ◽  
Dailun Hu ◽  
Janina E.E. Tirnitz-Parker ◽  
...  
Keyword(s):  
Nervous System ◽  
Dendritic Cells ◽  
Lymph Node ◽  
Immune System ◽  
Mesenteric Lymph Node ◽  
Immune Cell ◽  
Cell Interactions ◽  
Immunofluorescent Staining ◽  
Local Interactions ◽  
Mesenteric Lymph

<p>The central nervous system (CNS) influences the immune system in a general fashion by regulating the systemic concentration of humoral substances, whereas the autonomic nervous system communicates specifically with the immune system according to local interactions. Data concerning the mechanisms of this bidirectional crosstalk of the peripheral nervous system (PNS) and immune system remain limited. To gain a better understanding of local interactions of the PNS and immune system, we have used immunofluorescent staining of glial fibrillary acidic protein (GFAP), coupled with confocal microscopy, to investigate the non-myelinating Schwann cell (NMSC)-immune cell interactions in mouse mesenteric lymph nodes. Our results demonstrate i) the presence of extensive NMSC processes and even of cell bodies in each compartment of the mouse mesenteric lymph node; ii) close associations/interactions of NMSC processes with blood vessels (including high endothelial venules) and the lymphatic vessel/sinus; iii) close contacts/associations of NMSC processes with various subsets of dendritic cells (such as CD4<sup>+</sup>CD11c<sup>+</sup>, CD8<sup>+</sup>CD11c<sup>+ </sup>dendritic cells), macrophages (F4/80<sup>+</sup> and CD11b<sup>+</sup> macrophages), and lymphocytes. Our novel findings concerning the distribution of NMSCs and NMSC-immune cell interactions inside the mouse lymph node should help to elucidate the mechanisms through which the PNS affects cellular- and humoral-mediated immune responses or vice versa in health and disease.</p>

scholarly journals Crucial roles of B7-H1 and B7-DC expressed on mesenteric lymph node dendritic cells in the generation of antigen-specific CD4+Foxp3+ regulatory T cells in the establishment of oral tolerance

Blood ◽  
2010 ◽  
Vol 116 (13) ◽  
pp. 2266-2276 ◽  
Author(s):  
Tomohiro Fukaya ◽  
Hideaki Takagi ◽  
Yumiko Sato ◽  
Kaori Sato ◽  
Kawori Eizumi ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Lymph Node ◽  
Regulatory T Cells ◽  
Mesenteric Lymph Node ◽  
Oral Tolerance ◽  
Family Members ◽  
T Cell Response ◽  
Mesenteric Lymph ◽  
B7 Family

Abstract Oral tolerance is a key feature of intestinal immunity, generating systemic tolerance to fed antigens. However, the molecular mechanism mediating oral tolerance remains unclear. In this study, we examined the role of the B7 family members of costimulatory molecules in the establishment of oral tolerance. Deficiencies of B7-H1 and B7-DC abrogated the oral tolerance, accompanied by enhanced antigen-specific CD4+ T-cell response and IgG1 production. Mesenteric lymph node (MLN) dendritic cells (DCs) displayed higher levels of B7-H1 and B7-DC than systemic DCs, whereas they showed similar levels of CD80, CD86, and B7-H2. MLN DCs enhanced the antigen-specific generation of CD4+Foxp3+ inducible regulatory T cells (iTregs) from CD4+Foxp3− T cells rather than CD4+ effector T cells (Teff) relative to systemic DCs, owing to the dominant expression of B7-H1 and B7-DC. Furthermore, the antigen-specific conversion of CD4+Foxp3− T cells into CD4+Foxp3+ iTregs occurred in MLNs greater than in peripheral organs during oral tolerance under steady-state conditions, and such conversion required B7-H1 and B7-DC more than other B7 family members, whereas it was severely impaired under inflammatory conditions. In conclusion, our findings suggest that B7-H1 and B7-DC expressed on MLN DCs are essential for establishing oral tolerance through the de novo generation of antigen-specific CD4+Foxp3+ iTregs.

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