Orbita-Infektion mit Delftia acidovorans nach Katzenkratzer

Walter Johannes Smits; Heinz-Hubert Feucht; Frank Oellig; Bernhard Zöllner

doi:10.1055/a-1173-4598

Orbita-Infektion mit Delftia acidovorans nach Katzenkratzer

DMW - Deutsche Medizinische Wochenschrift ◽

10.1055/a-1173-4598 ◽

2020 ◽

Vol 145 (21) ◽

pp. 1559-1561

Author(s):

Walter Johannes Smits ◽

Heinz-Hubert Feucht ◽

Frank Oellig ◽

Bernhard Zöllner

Keyword(s):

16S Rrna ◽

Delftia Acidovorans ◽

Klinischer Befund

Zusammenfassung Anamnese und klinischer Befund Die 60-jährige Patientin wurde vor 34 Monaten von ihrer Katze am linken Augenlid gekratzt. Danach kam es zu chronischen, langsam progredienten Hautveränderungen mit sich verstärkenden Kopfschmerzen. Therapieversuche mit Kortison, Pimecrolimus, Pregabalin und Metamizol waren erfolglos. Nach 24 Monaten klagte sie über starken Bulbus-Schmerz am linken Auge, zunehmende Schmerzen bei Augenbewegungen und große Lichtempfindlichkeit. Es zeigten sich granulomatöse Papeln im Bereich des Auges. Untersuchungen und Diagnose Die interdisziplinären Untersuchungsbefunde und klinisch-chemischen Parameter waren unauffällig. Eine Biopsie aus dem Lidbereich erbrachte den Nachweis von Delftia acidovorans mittels bakterieller 16S-rRNA-PCR. Therapie und Verlauf Unter der Therapie mit Piperacillin/Tazobactam 3-mal 4,5 g/d i. v. für 10 Tage kam es zu einer raschen klinischen Besserung, sodass die Patientin nach 11 Tagen entlassen werden konnte. Nach weiteren 10 Monaten war sie rezidiv- und beschwerdefrei. Folgerung D. acidovorans ist bislang nicht als zoonotischer Erreger in Erscheinung getreten, sollte aber bei Verletzungen durch Tiere in die differenzialdiagnostischen Erwägungen einbezogen werden.

Download Full-text

Protein-induced conformational changes in 16S rRNA during assembly of the Escherichia Coli 30S ribosomal subunit: A STEM study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100128833 ◽

1987 ◽

Vol 45 ◽

pp. 910-911

Author(s):

M. Boublik ◽

V. Mandiyan ◽

J.F. Hainfeld ◽

J.S. Wall

Keyword(s):

16S Rrna ◽

Conformational Changes ◽

Ribosomal Proteins ◽

Structural Changes ◽

Ribosomal Subunit ◽

Compact Structure ◽

E Coli ◽

Freeze Dried ◽

16S Rna ◽

30S Subunit

The aim of this study is to understand the mechanism of 16S rRNA folding into the compact structure of the small 30S subunit of E. coli ribosome. The assembly of the 30S E. coli ribosomal subunit is a sequence of specific interactions of 16S rRNA with 21 ribosomal proteins (S1-S21). Using dedicated high resolution STEM we have monitored structural changes induced in 16S rRNA by the proteins S4, S8, S15 and S20 which are involved in the initial steps of 30S subunit assembly. S4 is the first protein to bind directly and stoichiometrically to 16S rRNA. Direct binding also occurs individually between 16S RNA and S8 and S15. However, binding of S20 requires the presence of S4 and S8. The RNA-protein complexes are prepared by the standard reconstitution procedure, dialyzed against 60 mM KCl, 2 mM Mg(OAc)2, 10 mM-Hepes-KOH pH 7.5 (Buffer A), freeze-dried and observed unstained in dark field at -160°.

Download Full-text

Imaging of ribosomal RNA-protein interactions by dark-field STEM

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100153221 ◽

1989 ◽

Vol 47 ◽

pp. 250-251

Author(s):

M. Boublik ◽

V. Mandiyan ◽

S. Tumminia ◽

J.F. Hainfeld ◽

J.S. Wall

Keyword(s):

Nucleic Acid ◽

16S Rrna ◽

Dark Field ◽

Radius Of Gyration ◽

Central Domain ◽

The Core ◽

16S Rna ◽

30S Subunit ◽

Core Particles

Success in protein-free deposition of native nucleic acid molecules from solutions of selected ionic conditions prompted attempts for high resolution imaging of nucleic acid interactions with proteins, not attainable by conventional EM. Since the nucleic acid molecules can be visualized in the dark-field STEM mode without contrasting by heavy atoms, the established linearity between scattering cross-section and molecular weight can be applied to the determination of their molecular mass (M) linear density (M/L), mass distribution and radius of gyration (RG). Determination of these parameters promotes electron microscopic imaging of biological macromolecules by STEM to a quantitative analytical level. This technique is applied to study the mechanism of 16S rRNA folding during the assembly process of the 30S ribosomal subunit of E. coli. The sequential addition of protein S4 which binds to the 5'end of the 16S rRNA and S8 and S15 which bind to the central domain of the molecule leads to a corresponding increase of mass and increased coiling of the 16S rRNA in the core particles. This increased compactness is evident from the decrease in RG values from 114Å to 91Å (in “ribosomal” buffer consisting of 10 mM Hepes pH 7.6, 60 mM KCl, 2 m Mg(OAc)2, 1 mM DTT). The binding of S20, S17 and S7 which interact with the 5'domain, the central domain and the 3'domain, respectively, continues the trend of mass increase. However, the RG values of the core particles exhibit a reverse trend, an increase to 108Å. In addition, the binding of S7 leads to the formation of a globular mass cluster with a diameter of about 115Å and a mass of ∽300 kDa. The rest of the mass, about 330 kDa, remains loosely coiled giving the particle a “medusa-like” appearance. These results provide direct evidence that 16S RNA undergoes significant structural reorganization during the 30S subunit assembly and show that its interactions with the six primary binding proteins are not sufficient for 16S rRNA coiling into particles resembling the native 30S subunit, contrary to what has been reported in the literature.

Download Full-text

Differentiation Of Clarias Batrachus, C. Gariepinus And Heteropneustes Fossilis By Pcr-Sequencing Of Mitochondrial 16s Rrna Gene

Journal of the Asiatic Society of Bangladesh Science ◽

10.3329/jasbs.v41i1.46190 ◽

2015 ◽

Vol 41 (1) ◽

pp. 51-58

Author(s):

Mohammad Shamimul Alam ◽

Hawa Jahan ◽

Rowshan Ara Begum ◽

Reza M Shahjahan

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Fish Larva ◽

16S Rrna Genes ◽

Rrna Genes ◽

Rrna Gene ◽

Valuable Insight ◽

Multiple Sequence ◽

Pcr Rflp ◽

Alternative Means

Heteropneustesfossilis, Clariasbatrachus and C. gariepinus are three major catfishes ofecological and economic importance. Identification of these fish species becomes aproblem when the usual external morphological features of the fish are lost or removed,such as in canned fish. Also, newly hatched fish larva is often difficult to identify. PCRsequencingprovides accurate alternative means of identification of individuals at specieslevel. So, 16S rRNA genes of three locally collected catfishes were sequenced after PCRamplification and compared with the same gene sequences available from othergeographical regions. Multiple sequence alignment of the 16S rRNA gene fragments ofthe catfish species has revealed polymorphic sites which can be used to differentiate thesethree species from one another and will provide valuable insight in choosing appropriaterestriction enzymes for PCR-RFLP based identification in future. Asiat. Soc. Bangladesh, Sci. 41(1): 51-58, June 2015

Download Full-text

Haemophilus parainfluenzaeInfective Endocarditis Diagnosed by Direct 16S rRNA Sequencing of Vegetation

Laboratory Medicine Online ◽

10.3343/lmo.2012.2.2.111 ◽

2012 ◽

Vol 2 (2) ◽

pp. 111

Author(s):

Sung-Hee Oh ◽

Min-Chul Cho ◽

Jae-Wook Kim ◽

Dongheui An ◽

Mun-Hui Jeong ◽

...

Keyword(s):

16S Rrna ◽

16S Rrna Sequencing ◽

Rrna Sequencing

Download Full-text

Marine mammals are natural hosts of Oceanivirga salmonicida, a bacterial pathogen of Atlantic salmon

Diseases of Aquatic Organisms ◽

10.3354/dao03478 ◽

2020 ◽

Vol 139 ◽

pp. 161-174

Author(s):

R Palmer ◽

GTA Fleming ◽

S Glaeser ◽

T Semmler ◽

A Flamm ◽

...

Keyword(s):

16S Rrna ◽

Atlantic Salmon ◽

16S Rrna Gene ◽

Marine Mammals ◽

Rrna Gene ◽

Bacterial Disease ◽

Common Dolphin ◽

Stenella Coeruleoalba ◽

Striped Dolphin ◽

Natural Hosts

During 1992 and 1993, a bacterial disease occurred in a seawater Atlantic salmon Salmo salar farm, causing serious mortalities. The causative agent was subsequently named as Oceanivirga salmonicida, a member of the Leptotrichiaceae. Searches of 16S rRNA gene sequence databases have shown sequence similarities between O. salmonicida and uncultured bacterial clones from the digestive tracts of marine mammals. In the current study, oral samples were taken from stranded dolphins (common dolphin Delphinus delphis, striped dolphin Stenella coeruleoalba) and healthy harbour seals Phoca vitulina. A bacterium with growth characteristics consistent with O. salmonicida was isolated from a common dolphin. The isolate was confirmed as O. salmonicida, by comparisons to the type strain, using 16S rRNA gene, gyrB, groEL, and recA sequence analyses, average nucleotide identity analysis, and MALDI-TOF mass spectrometry. Metagenomic analysis indicated that the genus Oceanivirga represented a significant component of the oral bacterial microbiomes of the dolphins and seals. However, sequences consistent with O. salmonicida were only found in the dolphin samples. Analyses of marine mammal microbiome studies in the NCBI databases showed sequences consistent with O. salmonicida from the common dolphin, striped dolphin, bottlenose dolphin Tursiops truncatus, humpback whale Megaptera novaeangliae, and harbour seal. Sequences from marine environmental studies in the NCBI databases showed no sequences consistent with O. salmonicida. The findings suggest that several species of marine mammals are natural hosts of O. salmonicida.

Download Full-text

ISOLASI, SELEKSI, DAN IDENTIFIKASI BAKTERI SELULOLITIK DARI RUMPUT LAUT Turbinaria sp. DAN Sargassum sp. SEBAGAI KANDIDAT PENDEGRADASI SERAT KASAR PAKAN IKAN

Jurnal Riset Akuakultur ◽

10.15578/jra.10.1.2015.51-60 ◽

2015 ◽

Vol 10 (1) ◽

pp. 51

Author(s):

Mulyasari Mulyasari ◽

Irma Melati ◽

Mas Tri Djoko Sunarno

Keyword(s):

Bacillus Subtilis ◽

16S Rrna

Kecernaan karbohidrat oleh ikan dapat ditingkatkan antara lain melalui penambahan bakteri selulolitik dalam pakan. Salah satu sumber bakteri selulolitik adalah rumput laut. Oleh karena itu, suatu penelitian dilakukan dengan tujuan untuk mendapatkan bakteri selulolitik dari rumput laut Turbinaria sp. dan Sargassum sp. Bakteri selulolitik diisolasi dengan metode pengenceran, streaking dan spreading pada media carboxymethylcellulose (CMC). Koloni yang didapat dimurnikan dan diseleksi dengan menggunakan uji aktivitas enzim selulase secara kualitatif (zona bening) dan kuantitatif, serta diidentifikasi secara biokimia dan molekuler menggunakan gen 16S-rRNA. Pada penelitian ini diperoleh 22 isolat murni bakteri dengan dua isolat yang mempunyai aktivitas selulolitik tertinggi, yaitu TS2b dan SS4b. Hasil uji biokimia dan karakterisasi secara molekuler menunjukkan bahwa kedua isolat tersebut adalah Bacillus subtilis dan B. megaterium.

Download Full-text