Comparative Chemical, Biological and Clinical Studies of 99mTc-Glucoheptonate and 99mTc-Dimercaptosucci- nate as Used in Renal Scintigraphy

1979 ◽  
Vol 18 (01) ◽  
pp. 40-45 ◽  
Author(s):  
M. Malešević ◽  
Lj. Stefanović ◽  
N. Vanlić-Razumenić
Keyword(s):  
Clinical Studies ◽  
Gamma Camera ◽  
Radiochemical Purity ◽  
Renal Cortex ◽  
Renal Scintigraphy ◽  
Time Intervals ◽  
Experimental Animals ◽  
Dynamic Studies

The renal radiopharmaceutical preparations 99mTc-DMS and 99mTc-GH were examined chemically, biologically and clinically. Both preparations are of high radiochemical purity. The biodistribution of both preparations was examined in experimental animals at different time intervals, from 15 min to 4 hr; the percentage of incorporation of 99mTc-DMS into kidneys is much higher (29.4% to 52.0%) than that of 99mTc-GH (12.80% to 22.20%). Both preparations accumulate to a greater extent in the renal cortex than in the medulla.The most suitable time for renal scintigraphy for "mTc-DMS is 90-150 min while for 99mTc-GH it is 60-90 min. It is concluded that 99mTc-DMS is more suitable for static scintigrams on the scanner and 99mTc-GH for dynamic studies with the gamma camera.

scholarly journals 10 Final Report on the Safety Assessment of Cholesterol

1986 ◽  
Vol 5 (5) ◽  
pp. 491-516 ◽  
Keyword(s):  
Clinical Studies ◽  
Safety Assessment ◽  
Final Report ◽  
Experimental Animals ◽  
Normal Metabolism ◽  
Hair Care ◽  
Uvb Exposure ◽  
High Doses ◽  
Skin Irritants

Cholesterol is used as an emulsifier in cosmetic skin and hair care products and eye and face makeup formulations at concentrations up to 5%. The normal metabolism and excretion of Cholesterol is well documented in man and experimental animals. Cholesterol is not a significant dermal or ocular irritant. Cholesterol does not appear to have any genotoxic activity in bacterial or mammalian cell in vitro mutagenic and transformation assays. High doses of Cholesterol were teratogenic in rats. Cholesterol has not been established as a promoter, cocarcinogen, or total carcinogen. Clinical studies to evaluate the safety of topically applied Cholesterol were restricted to products formulated with the ingredient. Most products were moisturizers containing 1.4% Cholesterol. The highest concentration of Cholesterol tested (6%) was evaluated in a modified prophetic test (110 subjects) and an RIPT (45 subjects); both assays had UVA and UVB exposure incorporated into the protocols. The Cholesterol-containing products were minimal to mild primary and cumulative skin irritants but not sensitizers or photosensitizers.

scholarly journals Implementing Curcumin in Translational Oncology Research

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5240
Author(s):  
Koraljka Gall Trošelj ◽  
Ivana Samaržija ◽  
Marko Tomljanović ◽  
Renata Novak Kujundžić ◽  
Nikola Đaković ◽  
...  
Keyword(s):  
Clinical Trials ◽  
Clinical Studies ◽  
Metabolic Reprogramming ◽  
Current Status ◽  
Strong Indication ◽  
Experimental Animals ◽  
Oncology Research ◽  
Modifying Effect ◽  
Detailed Presentation

Most data published on curcumin and curcumin-based formulations are very promising. In cancer research, the majority of data has been obtained in vitro. Less frequently, researchers used experimental animals. The results of several clinical studies are conclusive, and these studies have established a good foundation for further research focusing on implementing curcumin in clinical oncology. However, the issues regarding timely data reporting and lack of disclosure of the exact curcumin formulations used in these studies should not be neglected. This article is a snapshot of the current status of publicly available data on curcumin clinical trials and a detailed presentation of results obtained so far with some curcumin formulations. Phenomena related to the observed effects of curcumin shown in clinical trials are presented, and its modifying effect on gut microbiota and metabolic reprogramming is discussed. Based on available data, there is a strong indication that curcumin and its metabolites present molecules that do not necessarily need to be abundant in order to act locally and benefit systemically. Future clinical studies should be designed in a way that will take that fact into consideration.

Production of Meta-Iodobenzilguanidine, 123I Preparation for Medical Diagnostics

2015 ◽  
Vol 1084 ◽  
pp. 451-455
Author(s):  
Ilyas E. Slamkulov ◽  
Viktor S. Skuridin ◽  
Aleksey S. Semenov ◽  
Aleksandr A. Garapatsky
Keyword(s):  
Ammonium Sulfate ◽  
Radiochemical Purity ◽  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Medical Diagnostics ◽  
Phase Synthesis ◽  
Experimental Animals ◽  
Experimental Installation ◽  
Iodine 123

This paper demonstrates a method for production of the radiopharmaceutical meta-iodobenzilguanidin (MIBG) labeled with radionuclide iodine-123 (123I). An experimental installation has been developed and the conditions of solid-phase synthesis of the preparation have been described. The influence of the content of ammonium sulfate in the reaction mixture and the temperature and duration of dry synthesis on the process of labeling has been researched. It has been established experimentally that when the ratio of MIBG and ammonium sulfate is 1:4, it is efficient to carry out synthesis at the temperature of 150°C to rapidly obtain a preparation with radiochemical purity of over 97%. Biomedical tests of the synthesized preparation have been carried out in experimental animals. Its functional suitability for scintigraphic studies has been shown.

Pharmacology of Radioiodinated Hexadecenoic Acid – A Myocardial Imaging Agent

1984 ◽  
Vol 23 (02) ◽  
pp. 73-74 ◽  
Author(s):  
J. Zhang ◽  
Q.-M. Ji ◽  
Y.-C. Wang ◽  
D.-F. Xie ◽  
R.-L. Hua ◽  
...  
Keyword(s):  
Gamma Camera ◽  
Radiochemical Purity ◽  
Myocardial Imaging ◽  
Blood Levels ◽  
Hexadecenoic Acid ◽  
Myocardial Uptake ◽  
Bovine Serum Albumin Solution ◽  
Albumin Solution

Summary 123I- and 131I-labeled hexadecenoic acid (IHDA, radiochemical purity over 92%, dissolved in 6% bovine serum albumin solution) was investigated in vivo. ICR mice were administered IHDA via the tail vein. Maximum myocardial uptake (27.3 ± 5.1%) was reached about 0.5 min after the injection. The ratio of uptake in the heart to that in the lungs was 2.3, to that in liver 1.5 and to that in other organs 2.4 to 6.4. The dog myocardium was visualized distinctly within 3-5 min with a gamma camera after i.v. 131I-IHDA, and not interfered with by activities in the lungs, liver and other organs. The low blood levels at 20 min had little effect on the quality of the heart images.

scholarly journals History of the therapy of pernicious anemia

2013 ◽  
Vol 154 (44) ◽  
pp. 1754-1758
Author(s):  
András Jeney
Keyword(s):  
Blood Cell ◽  
Oral Administration ◽  
Pernicious Anemia ◽  
Clinical Studies ◽  
Mode Of Action ◽  
Vitamin B ◽  
Cell Regeneration ◽  
Experimental Animals ◽  
Therapeutic Value ◽  
History Of

Increased blood cell regeneration in exsanguinated experimental animals treated either with liver or with aqueous liver extracts was reported by Whipple and by Jeney and Jobling, respectively. These findings stimulated Minot and Murphy to provide evidence for the efficacy of liver against anaemia in clinical studies. After oral administration of liver (45–50 g per day) for 45 patients with anaemia perniciosa improvement of the hematological status was demonstrated. Consequently, for proving the therapeutic value of liver therapy Whipple, Minot and Murphy received Nobel price in 1934. The isolation of the antianemic factor from the liver has been succeded in 1948 and designated as vitamin B12. At the same time Lucy Wills applied yeast for the treatment of pregnant women with anemia related to undernourisment. The conclusions of this study inspired the discovery of folate. The detailed investigation of the mode of action of vitamin B12and folate enriched our knowledge in the area of pathophysiology and extended the clinical application of these two drugs. Orv. Hetil., 154 (44), 1754–1758.

A phantom for simulating fast dynamic studies on a gamma camera

1977 ◽  
Vol 22 (1) ◽  
pp. 87-89 ◽  
Author(s):  
F R Hudson ◽  
J B Davis ◽  
G T Mott
Keyword(s):  
Gamma Camera ◽  
Dynamic Studies ◽  
Fast Dynamic

Technetium-99m Labelling of the IOR CEA 1 Monoclonal Antibody: Evaluation of Different Methods

1997 ◽  
Vol 36 (06) ◽  
pp. 205-212 ◽  
Author(s):  
L. Gano ◽  
C. Fernandes ◽  
G. Cantinho ◽  
A. I. Santos ◽  
H. Pena ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Clinical Studies ◽  
Radiochemical Purity ◽  
High Antibody ◽  
Sds Page ◽  
Direct Labelling ◽  
Stannous Ion ◽  
Biological Distribution

Summary Aim: The aim of this study was to investigate the in vivo and in vitro properties of 99mTc labelled monoclonal antibody, IOR CEA 1 when radio-labelled by different methods. Methods: To achieve that purpose IOR CEA was directly radiolabeled via 2-mercaptoethanol (2-Me) and stannous ion (SnCI2) reduction and indirectly via the 2-iminothiolane (2-lm) conjugation. The resulting 99mTc-MoAbs were analysed for number of free sulfhydryl groups, chemical and radiochemical purity (checked by HPLC and SDS PAGE), immunoreactivity and biological distribution in mice. Results: Experimental results indicated a similar radiochemical purity and immunoreactivity for direct labelling methods and a decrease of both for 2-lm method. 2-Me antibody reduction led to a high antibody fragmentation as indicated by non-denaturing SDS PAGE analysis. Nevertheless SnCI2 and 2-lm labels revealed lower in vivo stability. Conclusion: 99mTc-(2-Me) IOR CEA presented favorable in vitro and in vivo properties. Therefore this label was compared to 99mTc-monoclonal antibody BW 431/26. Similar characteristics were found. Clinical studies also revealed identical biodistribution profile.

3Rs as part of preclinical neuropsychiatric translational crisis, and ARRIVE guidelines as part of solution

2019 ◽  
Vol 31 (6) ◽  
pp. 348-349 ◽  
Author(s):  
Aage Kristian Olsen Alstrup ◽  
Christian Sonne
Keyword(s):  
Systematic Reviews ◽  
Clinical Studies ◽  
Animal Studies ◽  
Brain Diseases ◽  
Preclinical Research ◽  
Experimental Animals ◽  
Accurate Reporting

AbstractObjective:The ongoing translational and reproducibility crisis dominates preclinical research today as results from animal studies often disappoint when transferred to human clinical studies. This problem is especially relevant in the field of brain diseases and translational neuropsychiatry.Methods:Here, we discuss if the 3R concept could be part of the translational crisis.Results:The focus has been on the second R, which is to reduce the variation between the experimental animals, so that the number of animals per study can be reduced. However, the risk of obtaining false results has also increased. We, therefore, recommend that researchers use a broader perspective as also suggest by Russell and Burch who founded the 3Rs when considering the 3R concept, which involves the translational aspects described in detail in their 3R book from 1959.Conclusion:This may together with systematic reviews and well-designed and well-performed animal studies and accurate reporting of the results indeed contribute to solving the translational crisis in preclinical research.

Electron microscopic radioautographic study of glycoprotein biosynthesis and renewal in the renal glomeruli of the rat

1978 ◽  
Vol 36 (2) ◽  
pp. 476-477
Author(s):  
A. Haddad ◽  
J-J. Lachat ◽  
R. P. Gonçalves
Keyword(s):  
Renal Cortex ◽  
Mesangial Cells ◽  
Electron Microscopic ◽  
Time Intervals ◽  
Light Microscope Level ◽  
Thin Sections ◽  
Main Site ◽  
Renal Glomeruli ◽  
Silver Grains ◽  
Electron Microscopic Radioautography

Biosynthesis, migration and renewal of glycoproteins were studied by radioautography in the renal glomeruli after administering L-3H-fucose to rats which were killed at several time intervals after injection, by perfusion of glutaraldehyde through the abdominal aorta. Small pieces of the kidney were post fixed in 0s04, dehydrated and embedded in Epon 812. Semithin and thin sections of the renal cortex were processed for light and electron microscopic radioautography, respectively. At the light microscope level (Fig. 1) it was observed that the main site of incorporation of 3H-fucose into glycoproteins was the paranuclear region of the visceral epithelial cells (podocytes). Weak paranuclear radioautographic reactions were also observed in endothelial and mesangial cells at 10 minutes after injection. At the longer time intervals these paranuclear reactions disappeared and the silver grains were mostly overlying the several components of the capillary wall. Silver grain counts showed that the peak of the silver grain density over the glomeruli occurred at 4 hours after injection; by 14 days the radioautographic reactions were almost negligible.

Sign in / Sign up

Export Citation Format

Share Document