International Quality Control In Blood Coagulation: Measurement Of Fibrinogen

1979 ◽  
Author(s):  
Jean Thomson ◽  
L Poller ◽  
K Yee
Keyword(s):  
Quality Control ◽  
Blood Coagulation ◽  
Thrombin Time

Fibrinogen estimations were included in four of the collaborative exercises from the above Centre between 1976-1978. More than thirty different techniques were employed by participants in over four hundred laboratories. The thrombin time, fibrinogen titre and turbidity techniques showed poor discrimination between normal and low fibrinogen levels compared with other routine methods e.g. Clauss technique, immunological and clot weight methods.

International, Quality Control in Blood Coagulation: Measurement of Fibrinogen

1979 ◽  
Author(s):  
Jean M. Thomson ◽  
L. Poller ◽  
K.F. Yee
Keyword(s):  
Quality Control ◽  
Blood Coagulation ◽  
Thrombin Time

Fibrinogen estimations were included in four of the collaborative exercises from the above Centre between 1976-1078. More than thirty different techniques were employed by participants in over four hundred laboratories. The thrombin time, fibrinogen titre and turbidity techniques showed poor discrimination between normal and low fibrinogen levels compared with other routine methods e.g. Clauss technique, immunological and clot weight methods.

Study of Haemostasis in Schoenlein-Henoch’s Syndrome

1975 ◽  
Author(s):  
Gy. Boros ◽  
J. Sámik ◽  
Ljubov Gofman ◽  
Judit Nagv ◽  
Gy. Deák ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Blood Coagulation ◽  
Fibrinolytic Activity ◽  
Capillary Permeability ◽  
Thrombin Time ◽  
Consumption Coagulopathy ◽  
Fibrin Deposition ◽  
Coagulation Defect ◽  
Capillary Resistance

Blood coagulation, thrombocyte function and capillary resistance were studied in 21 adult patients with Schoenlein-Henoch’s syndrome. 20 different tests were carried out at different stages of the disease. In 2 cases skin, in 1 mesocolon and in 10 kidneys were examined histologicaly (light microscopy, immunofluorescence, electron microscopy).Capillary permeability was increased at least once in every patient. All patients but 2 were characterized by hypercoagulability. In addition to thrombotic changes, an increase in labile fibrinogen was detected in the serum of 12 patients, and thrombin time was prolonged in 11 patients on 31 occasions. Plasma fibrinolytic activity was increased in 11 patients. Fibrin deposition was demonstrable in the skin of 2 in the mesocolon of 1 and in the kidneys of 7 patients.It is suggested that besides the capillaropathy a coagulation defect, resembling consumption coagulopathy is a characteristic of Schoenlein-Henoch’s syndrome.

Antihemostatic Effect of Hsien-Ho-T'sao (Agrimonia pilosa)

1984 ◽  
Vol 12 (01n04) ◽  
pp. 116-123 ◽  
Author(s):  
Jih-Pyang Wang ◽  
Mei-Feng Hsu ◽  
Che-Ming Teng
Keyword(s):  
Prothrombin Time ◽  
Blood Coagulation ◽  
Partial Thromboplastin Time ◽  
Bleeding Time ◽  
Fibrinogen Level ◽  
Platelet Rich Plasma ◽  
Water Extract ◽  
Activated Partial Thromboplastin Time ◽  
Thrombin Time

Bleeding time in rats was markedly prolonged after the adminstration of the water extract of Hsien-Ho-T'sao. This antihemostatic effect was more marked in the group of i.p. injection of the drug than in the group of p.o. administration for 2 to 7 consecutive days. Blood coagulation studies showed that plasma prothrombin time, activated partial thromboplastin time and stypven time were prolonged, while thrombin time adnd fibrinogen level were not changed. The thromboelastographic recording showed that reation time was prolonged and maximal elasticity of clot was decreased. In addition, ADP- and collagen- induced aggregations of platelet-rich plasma was suppressed. In conclusion, the prolongation of the bleeding time might be due to both anticoagulant and antiplatelet action of the drug.

Blood Coagulation Disturbances During Oliguria And Polyuria Of Acute Renal Failure In Man

1981 ◽  
Author(s):  
J Schrader ◽  
H Köstering ◽  
H Kaiser ◽  
P Kramer ◽  
F Scheler
Keyword(s):  
Renal Failure ◽  
Acute Renal Failure ◽  
Blood Coagulation ◽  
Daily Basis ◽  
Coagulation System ◽  
Factor V ◽  
Thrombin Time ◽  
Complex Activity ◽  
Ischemic Renal Failure ◽  
Postrenal Failure

The blood coagulation system makes a significant contribution to renal damage in many disease processes. Intrarenal coagulation appears to occur in a wide variety of diseases as a primary or secondary event. As there is evidence that intraglomerular coagulation is a significant factor in the development and maintenance of oliguria in acute ischemic renal failure, blood coagulation investigations were performed in 20 patients with acute renal failure of varied etiology. The investigations were done on a daily basis from the onset of oliguria (urine flow <20 ml/h)until serum creatinine declined to less than 2,0 mg%. Thus, we were able to detect changes in blood coagulation during oliguria and polyuria. We found an enhanced thrombin generation in both oliguria and polyria. Fibrin monomer complexes were significantly increased in both states, but more predominantly in polyuria. Factor VIII and alpha-1 antitrypsin activities were also elevated. PTT and r- and k-time in TEG were shortened more in polyuria than in oliguria, whereas fibrinogen was elevated more in oliguria than in polyuria. Factor XIII activity and prothrombin complex activity (Quick’s test) were lowered in both states, the lowest values of the former being found in polyuria, the lowest values of the latter in oliguria with a normalizing tendency in the following days. Fibrinolytic activity was also decreased. No significant changes were found in plasminogen, antithrombin III, alpha-2 macroglobulin, factor V and thrombin time. In summary, we found a hypercoagulability in these patients with acute renal failure, which was more predominant during polyuria and which correlated with the tendency to thrombosis and to shorter indwelling periods of i.v. catheters in this state. Consequently, the changes in blood coagulation of 3 patients with acute postrenal failure were not as significant as those found in the other patients. The treatment with anticoagulants in patients with acute renal failure will be discussed.

Influence Of Elastase On Blood Coagulation Factors: Studies In Vitro, In Rats And In Göttinger Miniatur Pigs

1981 ◽  
Author(s):  
U Kasten ◽  
U Artmann ◽  
T Kaethner ◽  
H Burchardi ◽  
H Köstering
Keyword(s):  
Blood Coagulation ◽  
Coagulation Factors ◽  
Bleeding Complications ◽  
Thrombin Time ◽  
Normal Range ◽  
Blood Coagulation Factors ◽  
Acute Respiratory Insufficiency ◽  
Coagulation Tests ◽  
Antifibrinolytic Drugs

The influence of blood coagulation factors in pat. with acute respiratory insufficiency of adults, especially of the so called “pancreatitis lungs” is still unknown. In order to find out the effect of elastase, possibly activated by trypsin in pat. with acute pancreatitis, on blood coagulation factors, we performed some studies. In vitro elastase induces in plasma and blood in correlation to the dosages Enhancement of thrombingeneration in the TGT, a shortening of PTT, Thrombin time and of r- and k-time in the TEG, a loss of fibrinogen and an increase of fibrinmono-mercomplexes. In another study, elastase (960 U/ kg b.w.) was injected intravenously in rats. 30 min. later there was found a loss of fibrinogen, number of platelets, Prothrombin and a prolongation of PTT and Thrombin time and an increase of fibrinomonomercomplexes, especially in these rats, which received beside elastase Kalikreininhibitors or antifibrinolytic drugs. After repeated injections (3 times within 30 h) we found histomorpholgically thrombi as well as bleeding complications. In another study we performed (150 min) an infusion of elastase (333 U/kg b.w./h) to 9 pigs. We determined a loss of fibrinogen of platelets, of F. II, F. VII and F. XIII, a prolongation of PTT. F. VIII and F. V remained within the normal range But there was found an enhancement of Thrombin generation in the TGT, too. Compariening the results of blood coagulation tests and of histomorphological findings, elastase induced a DIC. We have to discuss their influence on ARIA and “Pancreatic lungs”.

Studies On Coagulation Physiology In Perioperative Thromboembolism Prophylaxis In Surgery

1981 ◽  
Author(s):  
K Koppenhagen ◽  
A Häring ◽  
H Zühlke ◽  
A Wiechmann ◽  
M Matthes ◽  
...  
Keyword(s):  
Side Effects ◽  
Blood Coagulation ◽  
Low Dose ◽  
Combination Group ◽  
Double Blind Study ◽  
Thrombin Time ◽  
Thromboembolism Prophylaxis ◽  
Double Blind ◽  
Heparin Plasma ◽  
Low Dose Heparin

In a prospective and randomized double-blind study in 630 general surgery patients with different thromboembolism prophylaxis (130 patients with 5000 and 122 with 2500 I.U. of heparin, 128 with a fixed combination of 2500 I.U. of heparin and 0.5 mg dihydroergotamine ((2500-heparin-DHE)), 124 with 0.5 mg DHE and 126 with a placebo, 3 × daily) the influence of the medication on blood coagulation was examined by repeated pre-and post-operative determinations of blood-and coagulation parameters (activated partial thromboplastin time, heparin-plasma level, AT III, thromboplastin time, fibrinogen, thrombin time, thrombocyte aggregation test, β-thromboglobulin, Hb, erythrocytes, hematocrit, thrombocytes and differential blood picture). The frequency of postoperative thromboses and pulmonary embolisms was investigated by means of the radiofibrinogen test and lung scintigraphy. The results show that the lowest thromboembolism rate occurs in that patient collective in which there is in addition to the heparin application a simultaneous hemostatic effect of DHE. Side effects of the prophylaxis and influences on blood coagulation are dependent on the amount of heparin administered both in the single-drug low-dose treatment and in the combination therapy. The lowest rate of side effects was found in the group receiving 3×2500 I.U. of heparin and in the combination group 2500-heparin-DHE, but the combination treatment additionally affords the same thromboembolism protection as the routinely applied 3×5000 I.E. of low dose heparin.

Chicken prothrombin, thrombin, and fibrinogen

1961 ◽  
Vol 200 (5) ◽  
pp. 1013-1017 ◽  
Author(s):  
C. H. Bigland ◽  
D. C. Triantaphyllopoulos
Keyword(s):  
Prothrombin Time ◽  
Blood Coagulation ◽  
Thrombin Time ◽  
Bovine Thrombin ◽  
Chicken Brain ◽  
One Stage ◽  
Highly Sensitive ◽  
Avian Blood ◽  
The Mean ◽  
The One

Avian blood coagulation studies revealed the mean one-stage prothrombin time of plasma from 100 chickens, 10 weeks of age, as determined with chicken brain thromboplastin, to be 11.4 seconds, compared with 10–300 seconds found by other workers; the mean prothrombin content was 137% of human prothrombin levels, as determined by the one-stage prothrombin-time technique, but only 53% by the adsorption and elution technique used. Fibrinogen determinations on the same plasma revealed a mean of 346 mg/ 100 ml of plasma, compared with 250–400 for humans. Chicken thrombin was prepared which gave a mean thrombin time on the same, but buffered, plasma of 12 seconds. Coagulation of chicken plasma with chicken thrombin was found to be highly sensitive to variations in pH, being 12 seconds at pH 7.1, but lengthening to over 60 seconds at pH 7.7. Greatly increased clotting times of chicken plasma with bovine thrombin and with rabbit thromboplastin indicated evidence of species specificity.

scholarly journals The effects of fructose diphosphate on routine coagulation tests in vitro

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Tongqing Chen ◽  
Duan Chen ◽  
Lu Chen ◽  
Zhengxu Chen ◽  
Baolong Wang ◽  
...  
Keyword(s):  
Platelet Aggregation ◽  
Blood Coagulation ◽  
Detection System ◽  
Coagulation System ◽  
Thrombin Time ◽  
Aggregation Rate ◽  
Coagulation Testing ◽  
Coagulation Tests ◽  
Routine Coagulation

AbstractTo evaluate the effects of fructose diphosphate (FDP) on routine coagulation tests in vitro, we added FDP into the mixed normal plasma to obtain the final concentration of 0, 1, 2, 3, 4, 5, 6, 10, 15, 20, 25, 30 and 35 mg/mL of drug. Prothrombin time (PT), activated partial thromboplastin time (aPTT), fibrinogen (FBG) and thrombin time (TT) of samples were analyzed with blood coagulation analyzers from four different manufacturers(Sysmex, Stago, SEKISUI and Werfen) and their corresponding reagents, respectively. Before the experiment, we also observed whether there were significant differences in coagulation test results of different lots of reagents produced by each manufacturer. At the same time as the four routine clotting tests, the Sysmex blood coagulation analyzer and its proprietary analysis software were used to detect the change of maximum platelet aggregation rate in platelet-rich plasma after adding FDP (0, 1, 2, 3, 4, 5 and 6 mg/mL). The results of PT, aPTT and TT showed a FDP (0–35 mg/mL) concentration-dependent increase and a FBG concentration-dependent decrease. The degree of change (increase or decrease) varied depending on the assay system, with PT and aPTT being more affected by the Sysmex blood coagulation testing instrument reagent system and less affected by CEKISUI, TT less affected by CEKISUI and more affected by Stago, and FBG less affected by Stago and more affected by Sysmex. The results of PT, aPTT and TT were statistically positively correlated with their FDP concentrations, while FBG was negatively correlated. The correlation coefficients between FDP and the coagulation testing systems of Sysmex, Stago, Werfen and SEKISUI were 0.975, 0.988, 0.967, 0.986 for PT, and 0.993, 0.989, 0.990 and 0.962 for aPTT, 0.994, 0.960, 0.977 and 0.982 for TT, − 0.990, − 0.983, − 0.989 and − 0.954 for FBG, respectively. Different concentrations of FDP (0, 1, 2, 3, 4, 5 and 6 mg/mL) had different effects on the maximum aggregation rate of platelet induced by the agonists of adenosine diphosphate (ADP, 5 µmol/L), arachidonic acid (Ara, 1 mmol/L), collagen (Col, 2.5 µg/mL) and epinephrine (Epi,10 µmol/L), but the overall downward trend was consistent, that is, with the increase of FDP concentration, the platelet aggregation rate decreased significantly. Our experimental study demonstrated a possible effect of FDP on the assays of coagulation and Platelet aggregation, which may arise because the drug interferes with the coagulation and platelet aggregation detection system, or it may affect our in vivo coagulation system and Platelet aggregation function, the real mechanism of which remains to be further verified and studied.

scholarly journals Prominent changes in blood coagulation of patients with SARS-CoV-2 infection

2020 ◽  
Vol 58 (7) ◽  
pp. 1116-1120 ◽  
Author(s):  
Huan Han ◽  
Lan Yang ◽  
Rui Liu ◽  
Fang Liu ◽  
Kai-lang Wu ◽  
...  
Keyword(s):  
Blood Coagulation ◽  
Degradation Products ◽  
Control Group ◽  
Thrombin Time ◽  
Healthy Controls ◽  
Time Activity ◽  
Coagulation Function ◽  
Number Of Patients ◽  
Novel Coronavirus ◽  
D Dimer

AbstractBackgroundAs the number of patients increases, there is a growing understanding of the form of pneumonia sustained by the 2019 novel coronavirus (SARS-CoV-2), which has caused an outbreak in China. Up to now, clinical features and treatment of patients infected with SARS-CoV-2 have been reported in detail. However, the relationship between SARS-CoV-2 and coagulation has been scarcely addressed. Our aim is to investigate the blood coagulation function of patients with SARS-CoV-2 infection.MethodsIn our study, 94 patients with confirmed SARS-CoV-2 infection were admitted in Renmin Hospital of Wuhan University. We prospectively collect blood coagulation data in these patients and in 40 healthy controls during the same period.ResultsAntithrombin values in patients were lower than that in the control group (p < 0.001). The values of D-dimer, fibrin/fibrinogen degradation products (FDP), and fibrinogen (FIB) in all SARS-CoV-2 cases were substantially higher than those in healthy controls. Moreover, D-dimer and FDP values in patients with severe SARS-CoV-2 infection were higher than those in patients with milder forms. Compared with healthy controls, prothrombin time activity (PT-act) was lower in SARS-CoV-2 patients. Thrombin time in critical SARS-CoV-2 patients was also shorter than that in controls.ConclusionsThe coagulation function in patients with SARS-CoV-2 is significantly deranged compared with healthy people, but monitoring D-dimer and FDP values may be helpful for the early identification of severe cases.

The blood coagulation system of rats under the influence of laser radiation in different time parameters

2019 ◽  
Vol 11 (1) ◽  
pp. 19-26
Author(s):  
Oksana Ketsa ◽  
Mykhailo Marchenko ◽  
Nadiia Bodnarjuk
Keyword(s):  
Prothrombin Time ◽  
Laser Irradiation ◽  
Blood Coagulation ◽  
Laser Diode ◽  
Coagulation System ◽  
Simultaneous Increase ◽  
Thrombin Time ◽  
Blood Coagulation System ◽  
Group I ◽  
Time Parameters

The influence of different terms of laser irradiation on vascular-platelet and coagulation units of blood coagulation system is investigated. The rats were irradiated with a laser diode in the red spectrum (650 nm) with a power of 50 mW. Rats were divided into two groups: group I - rats, which were irradiated daily for 2 minutes; group II - rats, which were irradiated daily for 4 minutes. Euthanasia of animals was performed on the 7th and 14th days after the onset of irradiation. For the study of the blood coagulation system of rats used biochemical coagulogram with the analysis of the following indicators - the content of fibrinogen, thrombin and prothrombin time, activated partial thromboplastin time (APTT), platelet count. The content of lymphocytes and erythrocyte sedimentation rate were determined also. In the work used blood plasma with sodium citrate. It has been found that daily four-minute laser irradiation in the abdominal area for two weeks leads to a decrease in the concentration of factor I blood clotting (fibrinogen) and an increase in thrombin time. At the same time, prolongation of prothrombin time and APTT was detected, indicating a deficiency of other factors of blood coagulation - II, V, VII-XII, and the presence of anticoagulant status, which may be associated with inhibition of the internal pathway of clotting. Thrombocytopenia is probably caused by the increased destruction of platelets or their insufficient formation as a result of dysfunction of hematopoietic organs. The two-minute effect of laser irradiation does not affect the clinical and biochemical parameters of the blood coagulation system of rats after a weekly daily action of the laser diode. Two weeks irradiation is accompanied by processes of hypocoagulation, as evidenced by a decrease in fibrinogen levels, platelet counts, and a simultaneous increase in APTT, thrombin and prothrombin time.

Sign in / Sign up

Export Citation Format

Share Document