scholarly journals Prominent changes in blood coagulation of patients with SARS-CoV-2 infection

2020 ◽  
Vol 58 (7) ◽  
pp. 1116-1120 ◽  
Author(s):  
Huan Han ◽  
Lan Yang ◽  
Rui Liu ◽  
Fang Liu ◽  
Kai-lang Wu ◽  
...  
Keyword(s):  
Blood Coagulation ◽  
Degradation Products ◽  
Control Group ◽  
Thrombin Time ◽  
Healthy Controls ◽  
Time Activity ◽  
Coagulation Function ◽  
Number Of Patients ◽  
Novel Coronavirus ◽  
D Dimer

AbstractBackgroundAs the number of patients increases, there is a growing understanding of the form of pneumonia sustained by the 2019 novel coronavirus (SARS-CoV-2), which has caused an outbreak in China. Up to now, clinical features and treatment of patients infected with SARS-CoV-2 have been reported in detail. However, the relationship between SARS-CoV-2 and coagulation has been scarcely addressed. Our aim is to investigate the blood coagulation function of patients with SARS-CoV-2 infection.MethodsIn our study, 94 patients with confirmed SARS-CoV-2 infection were admitted in Renmin Hospital of Wuhan University. We prospectively collect blood coagulation data in these patients and in 40 healthy controls during the same period.ResultsAntithrombin values in patients were lower than that in the control group (p < 0.001). The values of D-dimer, fibrin/fibrinogen degradation products (FDP), and fibrinogen (FIB) in all SARS-CoV-2 cases were substantially higher than those in healthy controls. Moreover, D-dimer and FDP values in patients with severe SARS-CoV-2 infection were higher than those in patients with milder forms. Compared with healthy controls, prothrombin time activity (PT-act) was lower in SARS-CoV-2 patients. Thrombin time in critical SARS-CoV-2 patients was also shorter than that in controls.ConclusionsThe coagulation function in patients with SARS-CoV-2 is significantly deranged compared with healthy people, but monitoring D-dimer and FDP values may be helpful for the early identification of severe cases.

Effects or Blood Transrusion Amount on Coagulation Function and Safety in Patients with Severe Trauma

2021 ◽  
Vol 7 (5) ◽  
pp. 3957-3966
Author(s):  
Ke Yuan ◽  
Ling Ruan ◽  
Fang Zuo ◽  
Hui Liu ◽  
Jiewen Zhu ◽  
...  
Keyword(s):  
Partial Pressure ◽  
Blood Coagulation ◽  
Adverse Reactions ◽  
Massive Transfusion ◽  
Severe Trauma ◽  
Control Group ◽  
Study Group ◽  
Thrombin Time ◽  
Coagulation Function ◽  
Volume Level

Objective: To investigate the effects of different blood transfusion volumes on coagulation function and safety in patients with severe trauma.Patients and Methods: One hundred and ten cases with severe trauma were selected as the research subjects. They were admitted to our hospital from April 2016 to April 2018. Sixty patients who needed massive transfusion (1-1.5 times of their own blood volume level) were assigned to the study group, and another 50 patients who needed small amount of transfusion (less than their own blood volume level) were assigned to the control group. The blood coagulation function index, thrombelastogram index, blood gas analysis index and the influence of adverse reactions were compared between patients in the two groups before transfusion and 24h after transfusion. Results:Twenty fourhoursafter transfusion, prothrombin time (PT), activated partial thrombin time (APTT) and thrombin time (TT) were prolonged in both groups. Both platelet (PLT) and fibrinogen (FIB) decreased(p<0.05). Both response time of blood coagulation factor (R) and fibrin polymerization reaction time (K) decreased and were lowerin the study group than those in the control group, while fibrous protein aggregation function (Angle), platelet aggregation function (MA) elevated and were higherin the study group than those in the control group (p<0.05). pH in both groups decreased, oxygen partial pressure (P02) and carbon dioxide partial pressure (PC02) in both groups increased (p<0.05). PaC02 and Pa02 in the study group were higher than those in the control group, and pH was lower than that in the control group (p<0.05). The incidence of total adverse reactions in the study group was higher than that in the control group (p<0.05). Conclusion:ln conclusion, massive transfusion for patients with severe trauma could reduce the patient’s platelet and affect their coagulation function.

scholarly journals A Retrospective Analysis of the Coagulation Dysfunction in COVID-19 Patients

2020 ◽  
Vol 26 ◽  
pp. 107602962096486
Author(s):  
Xu Chen ◽  
Qinghua Wang ◽  
Min Xu ◽  
Chengbin Li
Keyword(s):  
Prothrombin Time ◽  
Retrospective Analysis ◽  
Sensitivity And Specificity ◽  
Area Under The Curve ◽  
Thrombin Time ◽  
Coagulation Function ◽  
Severe Group ◽  
New Biomarkers ◽  
Coagulation Dysfunction ◽  
D Dimer

To discuss the coagulation dysfunction in COVID-19 patients and to find new biomarkers to separate severe COVID-19 patients from mild ones. We use a retrospective analysis of 88 COVID-19 patients, and compare the coagulation function between severe and mild groups. We found the prothrombin time (PT), thrombin time (TT), D-dimer were significantly higher in the severe group ( P < 0.05), and the highest area under the curve (AUC) is 0.91 for D-dimer, while the AUC of PT and TT were 0.80 and 0.61 respectively. We identified that D-dimer has a better value in predicting patients who are likely to develop into severe cases, with the sensitivity and specificity were 84.4% and 88.8%, respectively. D-dimer may be a good biomarker to separate the severe COVID-19 patients from the mild ones.

scholarly journals Plasma levels of D-dimer and fibrin degradation products correlate with bullous pemphigoid severity: a cross-sectional study

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sijia Wang ◽  
Mei Lu ◽  
Zijun Zhao ◽  
Xueting Peng ◽  
Liang Li ◽  
...  
Keyword(s):  
Bullous Pemphigoid ◽  
Degradation Products ◽  
Eosinophil Cationic Protein ◽  
Control Group ◽  
Cationic Protein ◽  
Glucocorticoid Treatment ◽  
Cross Sectional ◽  
Fibrin Degradation Products ◽  
Eosinophil Counts ◽  
D Dimer

AbstractBullous pemphigoid (BP), the most frequent blistering dermatosis in the elderly, is associated with increased mortality. The severity of BP can be assessed by detecting the anti-BP180 immunoglobulin G (IgG) concentration, but the lab test is not available in many community clinics. BP patients are usually in a hypercoagulable state with increased levels of D-dimer and fibrin degradation products (FDPs). We aimed to evaluate the use of D-dimer and FDPs in assessing BP severity. We compared the levels of plasma D-dimer, plasma FDPs, eosinophil counts, eosinophil cationic protein, and serum anti-BP180 IgG concentration between 48 typical BP patients and 33 Herpes zoster (HZ) patients (control group). Correlational analyses were conducted to determine the relationships between the lab values and common BP severity markers. The plasma D-dimer and FDP levels were higher in BP patients than in HZ controls (D-dimer: 3297 ± 2517 µg/L vs. 569.70 ± 412.40 µg/L; FDP: 9.74 ± 5.88 mg/L vs. 2.02 ± 1.69 mg/L, respectively, P < 0.0001). Significant positive correlations were found between D-dimer/FDP levels and BP severity markers (i.e. anti-BP180 IgG concentration [D-dimer: r = 0.3928, P = 0.0058; FDP: r = 0.4379, P = 0.0019] and eosinophil counts [D-dimer: r = 0.3625, P = 0.0013; FDP: r = 0.2880, P = 0.0472]) in BP patients. We also found an association between FDP and urticaria/erythema lesions (r = 0.3016, P = 0.0372), but no other BPDAI components. In 19 BP patients with complete remission after systemic glucocorticoid treatment, D-dimer and FDP levels decreased post-therapy (D-dimer: 5559 ± 7492 µg/L vs. 1738 ± 1478 µg/L; P < 0.0001; FDP: 11.20 ± 5.88 mg/L vs. 5.13 ± 3.44 mg/L; P = 0.0003), whereas they did not in BP patients with treatment resistant. Plasma D-dimer and FDP are convenient markers to evaluate BP severity assistant on BPDAI and eosinophil counts. FDP is also helpful for inflammatory lesions in BP patients.

Changes in blood acid-base balance parameters and coagulation profile during diarrhea in calves

2013 ◽  
Vol 16 (3) ◽  
pp. 543-549 ◽  
Author(s):  
P. Sobiech ◽  
W. Rękawek ◽  
M. Ali ◽  
R. Targoński ◽  
K. Żarczyńska ◽  
...  
Keyword(s):  
Control Group ◽  
Thrombin Time ◽  
Acid Base Balance ◽  
Acid Base ◽  
Blood Samples ◽  
Coagulation Profile ◽  
At Iii ◽  
Base Balance ◽  
Diarrheic Calves ◽  
D Dimer

Abstract The purpose of this study was to investigate possible alterations in acid-base balance parameters and the coagulation profile in neonatal diarrheic calves. Twenty neonatal diarrheic and 20 clinically healthy neonatal calves aged between 1 week to 10 days were used. All blood samples were taken on the third day from the onset of diarrhea symptom. Venous blood samples were collected from each animal to determine platelet numbers, pH, pCO2, pO2, HCO3-, BE, O2SAT, ctCO2 and electrolytes (K+, Na+ and Cl-). Plasma samples were collected from each animal for the measurement of prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), the concentrations of fibrinogen, D-dimer and the activity of antithrombin III (AT III). Blood pH (7.19), BE (-10.6 mmol/l), HCO3 - (25.15 mmol/l), pO2 (3.33 kPa), O2SAT (24.12 %) were significantly lower and serum concentration of K+ (6.55 mmol/l) was significantly higher in diarrheic calves. These changes indicate the state of uncompensated metabolic acidosis with accompanying hyperkalemia. TT (32.05s) and APTT (39.9s) values were more prolonged in calves with diarrhea than in the control group. D-dimer (587.25 μg/l) concentrations were significantly increased while a visible drop in AT III (103.75%) activity and platelets counts (598 x109/l) were observed in diarrheic group of calves. The results suggest that a consumptive type of disseminated intravascular coagulation (DIC) developed in diarrheic calves.

scholarly journals Plasma Levels of D-dimer and Fibrin Degradation Products Correlate with Bullous Pemphigoid Severity: a Cross-sectional Study

2021 ◽  
Author(s):  
Sijia Wang ◽  
Mei Lu ◽  
Zijun Zhao ◽  
Xueting Peng ◽  
Liang Li ◽  
...  
Keyword(s):  
Bullous Pemphigoid ◽  
Degradation Products ◽  
The Elderly ◽  
Cross Sectional Study ◽  
Control Group ◽  
Glucocorticoid Treatment ◽  
Cross Sectional ◽  
Fibrin Degradation Products ◽  
Positive Correlations ◽  
D Dimer

Abstract Background Bullous pemphigoid (BP), the most frequent blistering dermatosis in the elderly, is associated with increased mortality. The severity of BP can be assessed by detecting the anti-BP180 immunoglobulin G (IgG) titer, but the lab test is not available in many community clinics. BP patients are usually in a hypercoagulable state with increased levels of D-dimer and fibrin degradation products (FDPs). ObjectivesTo evaluate the use of D-dimer and FDPs in assessing BP severity. Methods We compared the levels of plasma D-dimer, FDPs, eosinophils, and anti-BP180 IgG titer between 48 BP patients and 33 Herpes zoster (HZ) patients (control group). Correlational analyses were conducted to determine the relationships between the lab values and BP.ResultsThe plasma D-dimer and FDP levels were higher in BP patients than in HZ controls (D-dimer: 3297 ±2517 µg/L vs. 569.70 ±412.40 µg/L; FDP: 9.74 ±5.88 mg/L vs. 2.02 ±1.69 mg/L, respectively, P<0.0001). Significant positive correlations were found between D-dimer/FDP levels and BP severity markers (anti-BP180 IgG titer [D-dimer: r=0.3928, P=0.0058; FDP: r=0.4379, P=0.0019] and eosinophil counts [D-dimer: r=0.3625, P=0.0013; FDP: r=0.2880, P=0.0472]) in BP patients. We also found an association between FDP and urticaria/erythema lesions (r=0.3016, P=0.0372), but no other BPDAI components. In 19 BP patients with complete remission after systemic glucocorticoid treatment, D-dimer and FDP levels decreased post-therapy (D-dimer: 5559 ±7492µg/L vs. 1738 ±1478 µg/L; P<0.0001; FDP: 11.20 ±5.88 mg/L vs. 5.13 ±3.44 mg/L; P=0.0003), where as they did not in BP patients with treatment resistant.Conclusion Plasma D-dimer and FDP are convenient markers to evaluate BP severity.

scholarly journals The influence of hyperprolactinemia on coagulation parameters in females with prolactinomas

2014 ◽  
Vol 142 (5-6) ◽  
pp. 314-319 ◽  
Author(s):  
Milica Medic-Stojanoska ◽  
Gorana Mitic ◽  
Igor Mitic ◽  
Dragan Spasic ◽  
Nikola Curic ◽  
...  
Keyword(s):  
Young Female ◽  
Coagulation System ◽  
Control Group ◽  
Thrombin Time ◽  
Contributing Factors ◽  
Normal Range ◽  
Healthy Female ◽  
Before And After ◽  
Von Willebrand ◽  
D Dimer

Introduction. Currently there is little information on the effects of prolactin (PRL) on the coagulation and fibrinolytic systems. Objective. The aim of this study was to evaluate the effects of hypeprolactinemia on the parameters of the hemostatic system and activation of the coagulation system. Methods. We studied PRL levels, body mass index (BMI), values of activated partial thromboplastin time (aPTT), prothrombin time (PT), thrombin time (TT), D-dimer level, von Willebrand factor antigen (vWFAg) and fibrinogen in 15 young female patients with microprolactinomas before and after therapy and in 15 healthy female controls. Results. As expected, pretreatment PRL levels were significantly higher in patients than in controls (140.90?42.87 vs. 12.53?4.05 ng/ml; p<0.001). PT, although still in the normal range, was prolonged in patients with hyperprolactinemia as compared to the control group (13.53?1.39 vs. 12.65?0.53 s; p=0.03) and normalized after therapy (12.69?0.65 vs. 12.65?0.53 s; p=0.88). TT, although in normal range, was significantly shorter in the hypeprolactinemic patients than in the controls (14.34?4.52 vs. 17.21?1.35 s; p<0.025) and after treatment remained significantly shorter than in the controls (15.17?1.55 vs. 17.21?1.35 s; p<0.0001). D-dimer values before treatment in the patients with hyperproplactinemia were above the normal range (239.47?107.93 vs. 131.27?50.64 ng/ml, p=0.002) and decreased to normal values after therapy (239.47?107.93 vs. 146.60?39.15 ng/ml; p<0.001). D-dimer levels correlated with PRL (r=0.30) and the change in serum D-dimer values significantly correlated with the change in PRL levels during therapy (r=0.62). aPTT, vWFAg and fibrinogen were similar in patients and controls. Conclusion. In our study, increased thrombin generation that resulted in elevated D-dimer levels may be one of the contributing factors to the prethrombotic state in patients with hyperprolactinemia.

Binding Properties of Monoclonal Antibodies against Human Fragment D-Dimer of Cross-Linked Fibrin to Human Plasma Clots in an In Vivo Model in Rabbits

1989 ◽  
Vol 61 (02) ◽  
pp. 307-313 ◽  
Author(s):  
P Holvoet ◽  
J M Stassen ◽  
Y Hashimoto ◽  
D Spriggs ◽  
P Devos ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Human Plasma ◽  
Jugular Vein ◽  
Degradation Products ◽  
Control Group ◽  
Human Fibrinogen ◽  
Fab Fragments ◽  
Vein Segment ◽  
D Dimer

SummaryTwo (MA-15C5 and MA-8D3) out of approximately 500 monoclonal antibodies, obtained by fusion of P3X63-Ag8-6.5.3 myeloma cells with spleen cells of mice immunized with purified fragment D-dimer from human fibrin, demonstrated a more than 1,000-fold higher affinity for fragment D-dimer than for native fibrinogen. MA-15C5 was directed against a neoantigenic determinant only expressed in fragment D-dimer. MA-8D3 reacted equally well with fragment D-dimer of crosslinked fibrin and with fragment D of non-crosslinked fibrin but not with fragment D of fibrinogen. Both monoclonal antibodies did not crossreact with rabbit fibrin and its degradation products.The binding of 125I-labeled Fab fragments to human plasma clots, introduced and aged for 1 hr in the jugular vein of heparinized rabbits was studied. Following injection of an equimolar mixture of Fab fragments derived from MA-15C5 and MA-8D3, the clot to blood ratios of radioactivity increased from 3.2 ± 1.2(mean ± SD) at 4 hr to 7.2 ± 1.4 at 17 hr. The binding of Fab fragments of MA-15C5 and MA-8D3 was independent of the age (1 to 72hrs) of the clot and of heparin anticoagulation and was only slightly decreased (by 20%) in the presence of circulating human fibrinogen (90 mg/kg body weight) and of human crosslinked fibrin degradation products at a plasma concentration of 10 pg/ml. The binding of Fab fragments of MA-15C5 and MA-8D3 to occlusive human plasma clots in the femoral artery of rabbits was comparable to that of the non-occlusive human plasma clots in the jugular vein. The Fab fragments of MA-15C5 and MA-8D3, labeled with 123I to a specific activity of 10 μCi/pg were injected intravenously (3 μg/kg) in 72 rabbits with a nonocclusive 0.2 ml human plasma clot in the jugular vein and in 7 control rabbits that underwent the surgical procedure without clot formation. Total body scans performed at hourly intervals revealed a higher relative increase in gamma counts over the thrombus region in the group with thrombus as compared to controls: at 6 hr 54 ± 18 vs 16 ± 13% (mean ± SEM, p <0.1) and at12 hrs 35 ± 11 vs –7 ± 12 (p <0.05). The vein segment to blood ratios of 123I at 24 hrs were 6.6 ± 2.4 in the group with clot and 1.5 to 0.7 in the control group (p <0.01). We conclude that these Fab fragments may have a sufficiently high fibrin-affinity to allow in vivo thrombus localization by external scanning.

scholarly journals Blood coagulation studies in domestic pigs (Hanover breed) and minipigs (Goettingen breed)

1983 ◽  
Vol 17 (4) ◽  
pp. 346-349 ◽  
Author(s):  
H. Köstering ◽  
W.-P. Mast ◽  
T. Kaethner ◽  
K. Nebendahl ◽  
W. H. Holtz
Keyword(s):  
Blood Coagulation ◽  
Degradation Products ◽  
Thrombin Time ◽  
Domestic Pig ◽  
Thrombocyte Count ◽  
Domestic Pigs ◽  
Fibrin Degradation Products

Blood coagulation studies were performed on 60 Hanover domestic pigs ready for slaughter and a total of 84 Goettingen minipigs. A tendency to hypercoagulability was found in these pigs, expressed in a shortened PTT, r- and k-time in the thromboelastogram and a slightly reduced plasminogen and plasmin level. Many values were similar to those of man. Thrombin time was longer. This may be due more to enhanced formation of fibrin degradation products (anti-thrombin VI) than to greater amounts of endogenous heparin being released. The longer reptilase and thrombin coagulase times support this. The thrombocyte count differed only slightly. The findings suggest that the Goettingen minipig and Hanover domestic pig are suitable animals for comparative blood coagulation studies.

scholarly journals Sleep and Prospective Memory: A Retrospective Study in Different Clinical Populations

2020 ◽  
Vol 17 (17) ◽  
pp. 6113 ◽  
Author(s):  
Lorenzo Tonetti ◽  
Miranda Occhionero ◽  
Michele Boreggiani ◽  
Andreas Conca ◽  
Paola Dondi ◽  
...  
Keyword(s):  
Sleep Quality ◽  
Prospective Memory ◽  
Control Group ◽  
Poor Sleep ◽  
Clinical Group ◽  
Healthy Controls ◽  
Time Activity ◽  
Clinical Populations

Prospective memory (PM) is essential in everyday life because it concerns the ability to remember to perform an intended action in the future. This ability could be influenced by poor sleep quality, the role of which, however, is still being debated. To examine the role of sleep quality in PM in depth, we decided to perform a retrospective naturalistic study examining different clinical populations with a primary sleep disorder or comorbid low sleep quality. If sleep is important for PM function, we could expect poor sleep to affect PM performance tasks both directly and indirectly. We examined a total of 3600 nights, recorded using actigraphy in participants belonging to the following groups: primary insomnia (731 nights); narcolepsy type 1 (1069 nights); attention deficit hyperactivity disorder (152 nights in children and 239 in adults); severe obesity (232 nights); essential hypertension (226 nights); menopause (143 nights); healthy controls (808 nights). In a naturalistic activity-based PM task, each participant originally wore an actigraph around the non-dominant wrist and was requested to push the event-marker button at two specific times of day: bedtime (activity 1) and get-up time (activity 2). Each clinical group showed significantly lower sleep quality in comparison to the control group. However, only narcolepsy type 1 patients presented a significantly impaired PM performance at get-up time, remembering to push the event-marker button around half the time compared not only to healthy controls but also to the other clinical groups. Overall, the present results seem to point to sleep quality having no effect on the efficiency of a naturalistic activity-based PM task. Moreover, the data indicated that narcolepsy type 1 patients may show a disease-specific cognitive deficit of PM.

scholarly journals Tranexamic Acid Combined with Compression Bandage Following Total Knee Arthroplasty Promotes Blood Coagulation: A Retrospective Analysis

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Guo-hua Li ◽  
Li-ming Ding ◽  
Lei Sun ◽  
Fu Wang
Keyword(s):  
Total Knee Arthroplasty ◽  
Tranexamic Acid ◽  
Knee Arthroplasty ◽  
International Normalized Ratio ◽  
Control Group ◽  
Thrombin Time ◽  
Compression Bandage ◽  
Observation Group ◽  
Total Knee ◽  
D Dimer

Objective. This paper included a retrospective review of the effect of tranexamic acid (TXA) combined with pressure bandaging on hemostasis of patients who received a unilateral total knee arthroplasty (TKA) from 2017 to 2019. Methods. A total of 197 patients undergoing TKA were chosen to be classified into 2 groups, the compression bandage control group and compression bandage combined with TXA observation group. The patients received blood routine examination when they were in the 1st, 3rd, and 6th days of before and postoperation. Some parameters, such as hemoglobin (Hb), C-reactive protein (CRP), D-dimer value, fibrinogen, prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), international normalized ratio (INR), and erythrocyte sedimentation rate (ESR), were also investigated. Results. In our research, the mean age was 66.54 ± 7.95 years. No difference was found in patient sex ( P = 0.876 ) and age ( P = 0.749 ) between groups. No differences were found in the levels of Hb, fibrinogen, TT, and INR between the 2 groups at each period ( P > 0.05 ). The difference of PT was significantly different on the 1st day ( P = 0.011 ), 3rd day ( P = 0.010 ), and 6th day ( P = 0.004 ) after surgery. Besides, the changes in APTT in observation group were clearly higher compared with the control group on the 3rd day ( P = 0.001 ) and 6th day ( P = 0.001 ). On the 3rd and 6th days after operation, the CRP level of the two groups increased continuously, and the CRP level was significantly higher in the observation group in comparison with the control group ( P = 0.008 , P = 0.010 ). On 1st and 3rd days after surgery, compared to the control group, the D-dimer level of patients in the observation group was distinctly fewer ( P = 0.001 , P = 0.027 ). Conclusion. TXA combined with compression bandage is a potential option for the reduction of bleeding after TKA.

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