scholarly journals Role of EGFR and HER-2/NEU Expression in Gall Bladder Carcinoma

2021 ◽  
Author(s):  
Chhanda Das ◽  
Madhumita Mukhopadhyay ◽  
Srijana Subba ◽  
Ashis Kumar Saha ◽  
Biswanath Mukhopadhyay
Keyword(s):  
Gall Bladder ◽  
Bladder Carcinoma ◽  
Tertiary Care ◽  
Molecular Therapy ◽  
Tumor Type ◽  
Care Hospital ◽  
Gall Bladder Carcinoma ◽  
Poorly Differentiated ◽  
Her 2 ◽  
Well Differentiated

Abstract Background Gall bladder carcinoma (GBC) is the most common malignancy of the biliary tract. Being known for its geographical and racial variations, and compared with the global statistics, its incidence is higher in the Indian subcontinent, mainly in the northern and eastern regions, accounting for 80 to 95% of cases. Aims and Objectives This study was conducted to evaluate the clinic-pathological spectrum and expression of EGFR and HER-2/NEU in GBCs and to understand their relation to prognosis, paving the way for targeted therapies for better treatment outcomes and patient survival. Materials and Methods This is a prospective study performed in a tertiary care hospital in 30 resected specimens of GBC cases recorded in our Department of Pathology from November 2017 to November 2019. Clinical history including the radiological reports and demographic parameters were included in the study pro forma. Immunohistochemical (IHC) staining for EGFR and HER-2/NEU was performed on all the selected cases. Clinicopathologic parameters like age, sex, histologic type, perineural, and lymphovascular invasion were compared and correlated with EGFR and HER-2/NEU status. Results Expression of EGFR was found in 93.33% of cases, which showed a highly significant correlation with histological tumor type (p = 0.000). HER-2/NEU expression was found in 56.66% of cases, which also showed a significant correlation with histological tumour type (p = 0.021). We found most of the cases with strong EGFR immunoreactivity (3+) were poorly differentiated tumors and most of the cases showing weak immunoreactivity for EGFR (1+) were well-differentiated. Conversely, in case of HER-2/NEU immunoreactivity, strong staining (3+) was seen in well-differentiated tumors and weak staining (1+) in poorly differentiated tumors. A significant correlation was also found between EGFR and HER-2/NEU expression (p = 0.000) and between cholelithiasis and EGFR expression (p = 0.033). Conclusion EGFR is expressed in most cases of GBC. Its expression is more in poorly differentiated carcinomas as compared to the well-differentiated carcinomas, whereas HER-2/NEU expression is more in well-differentiated carcinomas. Therefore, they may serve as independent prognostic factors and also as targets for molecular therapy in GBCs.

scholarly journals EGFR and HER2neu Expression in Gall Bladder Carcinoma and Its Association with Clinicopathological Parameters - An Institutional Experience from North India

2021 ◽  
Vol 6 (1) ◽  
pp. 57-65
Author(s):  
Inara Abeer ◽  
Sabina Khan ◽  
Mohd. Jaseem Hasan ◽  
Musharraf Hussain
Keyword(s):  
Gall Bladder ◽  
Bladder Carcinoma ◽  
North India ◽  
Clinicopathological Parameters ◽  
Advanced Stage ◽  
Gall Bladder Carcinoma ◽  
Egfr Expression ◽  
Poorly Differentiated ◽  
Well Differentiated ◽  
Biliary Tract Malignancy

Objective: Gallbladder cancer (GBC) is the most common biliary tract malignancy found worldwide with very high incidence in North India especially Delhi region. It is characterized by poor prognosis and ineffective treatment especially in advanced stage. The aim of this study was to evaluate EGFR and HER2/neu immunoexpression in cancer patients and to correlate it with the clinicopathological parameters so as to identify GBC patients who can benefit from targeted therapy.Methods: Present study was conducted in the Department of Pathology, Hamdard Institute of Medical Sciences & Research, New Delhi. A total of 40 cases of Gallbladder carcinoma (GBC) were evaluated for Immunohistochemical expression of EGFR and HER2/neu. Clinicopathological parameters of GBC were studied and correlated with immunoexpression of EGFR and HER2 /neu. Result: The mean age of the GBC patients was 55.9 years with 90% being females. On histopathology, 34(85%) cases were conventional adenocarcinoma. The EGFR expression was positive in 29/40 cases (72.5%). It was significantly more positive in poorly differentiated grade and advanced stages of gall bladder carcinoma (P<0.05). The expression of HER2/neu was positive in 13/40 cases (32.5%). It was significantly more positive in well differentiated gall bladder carcinoma (P<0.05). Immunoexpression of EGFR was inversely related with HER2/neu expression and this association was statistically significant.Conclusion: Among GBC patients, EGFR expression and HER2/neu expression was 72.5% and 32.5%, respectively. Significant EGFR expression was seen in poorly differentiated and advanced stage cancers while significant HER2/neu expression was seen in well differentiated gall bladder carcinomas. To conclude, these two markers HER2/neu and EGFR can be used as predictive and prognostic markers respectively, with rationale to further explore the use of anti-HER2 and anti- EGFR therapy in gall bladder cancer.

scholarly journals Frequency of Gall Bladder Carcinoma among Patients of Cholelithiasis at Tertiary Care Hospital

2020 ◽  
pp. 20-24
Author(s):  
Mairaj Muhammad Khan ◽  
Agha Taj Mohammed ◽  
Rasool Bux Behan ◽  
Sohail Yousif
Keyword(s):  
Gall Bladder ◽  
Bladder Carcinoma ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Abdominal Ultrasound ◽  
Stone Disease ◽  
Care Hospital ◽  
Gall Bladder Carcinoma ◽  
Diagnostic Laboratory ◽  
Female Ratio

Objective: To determine the frequency of carcinoma of Gall Bladder in Hospitalized patients undergoing surgeries for cholelithiasis at tertiary care Hospital. Study Setting: General surgery department of Muhammad Medical College Mirpurkhas. Study Design: Descriptive.  Study Duration: From 2018 to 2019. Methodology: All the patients of cholelithiasis diagnosed via trans-abdominal ultrasound, those who underwent cholecystectomies and either of gender were included. After surgeries, specimens immediately were sent to the Hospital diagnostic laboratory to evaluate the gall bladder carcinoma. The information obtained was noted on a pre-designed proforma. Results: A total 200 patient of gall bladder stone disease were observed who underwent cholecystectomies. Average age was 53.8+5.62 years and male to female ratio was 1:2.5.  Incidence of carcinoma of gall bladder was 4%, which was significantly associated to gall bladder mass, chronic calculus cholecystitis and porcelain gall bladder (p=0.001).  Conclusion: The incidence of the gall bladder cancer in the study population was 4% and its relationship with gall bladder diseases (chronic cholecystitis and porcelain) was found to be significant.

scholarly journals Spectrum of computed tomography findings in gall bladder carcinoma patients: a retrospective observational study from tertiary care oncology setup

2020 ◽  
Vol 7 (7) ◽  
pp. 2091
Author(s):  
Palwasha Gul ◽  
Pari Gul ◽  
Omer Altaf ◽  
Talha Yaseen Kaimkhani ◽  
Hira Asghar
Keyword(s):  
Computed Tomography ◽  
Gall Bladder ◽  
Bladder Carcinoma ◽  
Bladder Wall ◽  
Tertiary Care ◽  
Research Centre ◽  
Therapeutic Window ◽  
Imaging Findings ◽  
Gall Bladder Carcinoma ◽  
Contrast Enhanced Ct

Background: Gallbladder carcinoma (CA) is the most common primary biliary carcinoma and fifth most common malignancy of the gastrointestinal tract. Patients are usually asymptomatic early in the course of the disease and as a result, therapeutic window is usually missed. Authors discuss spectrum of imaging findings in CA gall bladder with multidetector computed tomography (CT) as modality of choice.Methods: Retrospective cross-sectional study conducted at Shaukat Khanum memorial hospital and research centre. Data from January 2019 to January 2020 which included 70 patients was collected from HIS. Patients with imaging findings of CA gall bladder were examined who had undergone contrast enhanced CT. Institutional review board approved this study.Results: Out of 70 cases in this study, cholelithiasis was present in 60%. The commonest MDCT finding was mass replacing GB (78.6%), followed by eccentric/diffuse gall bladder wall thickening. Extension to porta hepatis was noted in 25 (35.7%) cases. Antroduodenal involvement was noted in 11 (15.8%), hepatic flexure involvement in 8 (11.4%). Vascular encasement/thrombosis of the portal vein noted in 6 (8.6%). Metastasis at presentation was seen in 16 patients i.e., 22.9%. Majority patients presented with stage III and IV disease.Conclusions: Single-center tertiary care oncology hospital study gives insight about imaging findings of carcinoma gallbladder. Authors conclude that multidetector CT is the diagnostic tool of choice in detection of gall bladder carcinoma, locoregional disease, distant metastasis and hence operability and non-operability of the disease.

scholarly journals A histopathological evaluation of gall bladder cancer with special reference to HER2/neu and E-cadherin in Southern part of Assam

2021 ◽  
Vol 9 (5) ◽  
pp. 1368
Author(s):  
Madhusmita Choudhury ◽  
Gargi Roy Choudhury ◽  
Monoj K. Deka ◽  
Shah A. Sheikh
Keyword(s):  
Bladder Cancer ◽  
Gall Bladder ◽  
Bladder Carcinoma ◽  
Prognostic Significance ◽  
Gall Bladder Cancer ◽  
Clinicopathological Parameters ◽  
Gall Bladder Carcinoma ◽  
Female Ratio ◽  
Her 2 ◽  
E Cadherin

Background/Objective: Gall bladder carcinoma (GBC) is an aggressive malignancy with high mortality and aggressive course, with palliation as the only available option. The signs and symptoms of gall bladder arcinoma are not specific and often present late. Diagnosis is, therefore, often made at advanced stage with poorer outcomes. Identifying biomarkers and cancer specific cellular targets, that will pave the way for novel therapeutic approaches and early diagnosis for gall bladder carcinoma, is urgently needed. Proto-oncogenes (HER-2) and E-Cadherin are commonly deregulated in gallbladder cancer (GBC). This study evaluates the prognostic significance of HER-2 and E-Cadherin in GBC patients in Silchar Medical College.Our main objective was to evaluate frequency of HER-2/neu overexpression in GBC and to seek its correlation, if any with conventional clinicopathological parameters and survival.Methods: A total of 168 cases were received and evaluated for Gall Bladder cancer and control specimens were prospectively collected from 2018-2020. Immunohistochemical staining was done using monoclonal antibodies to semiquantitatively evaluate HER-2 and E-Cadherin protein expression. The criterion for HER-2 and E-Cadherin positivity was set at 10% and >5% tumor cells showing complete, membranous staining. Clinicopathological correlations were drawn with major clinical outcomes. Results: It was observed that out of 168 cases the male to female ratio is 1:5 with highest number of cases in the age group of 50-59 , i.e., 70 cases with 41.7%. The most common location in this study was fundus with 69% of cases (116 out 0f 168), most commonly presented as biliary colic with 56 number of cases. Grading was also done in 168 cases where most number of cases were moderately differentiated with 86 number of cases with a percentage of 51%. Expression of Her2Neu and E-Cadherin was evaluated where highest number of cases were seen with 1+ score  in the IHC expressions of both the markers with 81 and 61 number of cases respectively. Conclusion: The increasing global incidence, late presentation leading to poor prognosis and lack of effective therapy make the management of gall bladderv carcinoma really challenging. Our study shows the abnormal expression of HER-2 and E-Cadherin expression in gall bladder carcinoma patients in Southern Assam and suggests that  these two markers can be used for potential tool for early detection of gall bladder carcinoma and also can be used for targeted therapy in gall bladder carcinoma.

New scoring system for differentiating xanthogranulomatous cholecystitis from gall bladder carcinoma: a tertiary care centre experience

2016 ◽  
Vol 88 (1-2) ◽  
pp. E34-E39 ◽  
Author(s):  
Kishore Rajaguru ◽  
Siddharth Mehrotra ◽  
Shailendra Lalwani ◽  
Vivek Mangla ◽  
Naimish Mehta ◽  
...  
Keyword(s):  
Gall Bladder ◽  
Bladder Carcinoma ◽  
Scoring System ◽  
Tertiary Care ◽  
Xanthogranulomatous Cholecystitis ◽  
Care Centre ◽  
Tertiary Care Centre ◽  
Gall Bladder Carcinoma ◽  
New Scoring

scholarly journals Study of cholecystectomy specimens over a period of one year in tertiary care centre

2017 ◽  
Vol 5 (3) ◽  
pp. 916
Author(s):  
I. Vijaya Bharathi ◽  
P. Urmila Devi ◽  
A. Bhagya Lakshmi
Keyword(s):  
Bladder Cancer ◽  
Gall Bladder ◽  
Bladder Carcinoma ◽  
Histopathological Examination ◽  
Tertiary Care ◽  
Gall Bladder Cancer ◽  
Tertiary Care Centre ◽  
Gall Bladder Carcinoma ◽  
Hematoxylin And Eosin ◽  
One Year

Background: Gall bladder is among the most common surgically resected organs with various neoplastic and non-neoplastic lesions. Chronic chocystitis is the most commonly encountered lesion in India and worldwide and 78-90% are associated with calculi. Gall bladder cancer constitutes 0.5% to 1.09% including both suspected and incidental diagnosis. The objective of present study was to evaluate the various lesions of Gall bladder and the importance of grossing and histopathological examination of every cholecystectomy specimen in order to diagnose the incidental gall bladder cancer (IGBC).Methods: A Retrospective study of cholecystectomy specimens for a period of one year, May 2015 to June 2016 was carried out. 252 Cholecystectomy specimens were received and all of them subjected for histopathological examination and the sections stained with routine Hematoxylin and Eosin stain.Results: Commonest age group being 21-40years (41%) followed by 41-60years (40%). Gall bladder (GB) lesions are more common in females 175 (70%) and M:F ratio 1:2.2.Most commonest lesions were Chronic calculous cholecystitis constituting 155 cases (61%) followed by chronic cholecystitis 52 cases (21%). Cholelithiasis was associated with both Acute and Chronic Cholecytitis constituting 67%. Acute calculous cholecystits constitute 13 cases (5%) and Acute cholecystitis constitutes 10 cases (4%). In Congenital anomalies, 3 cases (1.2%) of Biliary atresia and 4 cases (1.2%) of choledochal cyst were diagnosed. 4 cases (1.6%) of Gall bladder carcinoma was diagnosed. Among these 3 cases were incidental gall bladder carcinoma (IGBC).Conclusions: cholecystectomy specimens should be subjected for histopathological examination to study various lesions of GB and to detect unsuspected incidental gall bladder cancer as cholecystectomy itself is the treatment for gall bladder cancer and also other lesions of GB associated with cholelithiasis.

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