A novel triterpene glycoside from the sea cucumber Holothuria atra

This study aimed at evaluating the antibiofilm activity of the Red Sea metabolites from green alga Avrainvillea amadelpha, sea cucumber Holothuria atra and costal plant Sarcocornia fruticosa against three biofilm bacterial strains isolated from Jeddah coast. Free fatty acids (FFAs) and other lipoidal matters were extracted from these organisms and analyzed by GC-MS. The composition of lipoidal fractions showed that A. amadelpha is rich by 74% saturated FAs, while sea cucumber H. atra revealed high content (60%) of unsaturated FAs. Palmitic acid is the major FA component in all species ranging from 14.5 to 26.7%. Phytol, sterols and hydrocarbons (C8-C29) were represented in the alga A. amadelpha as high contents with values 25.8, 21.9 and 18.5%, respectively. The extracts and lipoidal contents showed biofilm inhibitory activity against the isolated bacterial strains, where the unsaponified lipoidal fraction of S. fruticosa exhibited highest inhibitory activity against Planomicrobium sp. at concentration of 200 µg/mL.

Download Full-text

A new triterpene glycoside from sea cucumber Holothuria leucospilota

Chinese Chemical Letters ◽

10.1016/j.cclet.2006.12.027 ◽

2007 ◽

Vol 18 (2) ◽

pp. 161-164 ◽

Cited By ~ 23

Author(s):

Hua Han ◽

Yang Hua Yi ◽

Ling Li ◽

Xiao Hua Wang ◽

Bao Shu Liu ◽

...

Keyword(s):

Triterpene Glycoside ◽

Sea Cucumber

Download Full-text

Isolation of sea cucumbers’s (Holothuria atra) secondary metabolite using column-chromatography technique

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/869/1/012010 ◽

2021 ◽

Vol 869 (1) ◽

pp. 012010

Author(s):

S Agustina ◽

S Bella ◽

S Karina ◽

I Irwan ◽

M Ulfah

Keyword(s):

Secondary Metabolites ◽

Ethyl Acetate ◽

Secondary Metabolite ◽

Column Chromatography ◽

Extraction Method ◽

Sea Cucumber ◽

Sea Cucumbers ◽

Marine Chemistry ◽

Holothuria Atra ◽

Metabolite Content

Abstract Identification of sea cucumbers from Benteng Inong Balee, Aceh Besar and their phytochemistry screening were conducted in December 2020 to January 2021 at Laboratory of Marine Chemistry and Fisheries Biotechnology, Universitas Syiah Kuala. The purpose of this study was to identify the species of sea cucumbers and its secondary metabolite content using phytochemistry screening and column chromatography. The species of sea cucumbers that were identified was Holothuria atra. The extraction method used in sea cucumber extraction was maceration method, while the separation of secondary metabolites used column-chromatography with eluent of n-hexane : ethyl acetate (8:4). The results showed that secondary metabolites obtained from phytochemical tests were flavonoids, saponins and triterpenoids.

Download Full-text

Sea cucumber community in the coastal area of the Bahoi Village,West Likupang Sub-district, North Minahasa District

JURNAL ILMIAH PLATAX ◽

10.35800/jip.8.1.2020.27601 ◽

2020 ◽

Vol 8 (1) ◽

pp. 37

Author(s):

Andia Tri Fritama Lumbu ◽

Lawrence J. L. Lumingas ◽

Gaspar D. Manu

Keyword(s):

Sea Cucumber ◽

Diversity Index ◽

Similarity Index ◽

Sampling Technique ◽

Distribution Patterns ◽

Line Transect ◽

Dominance Index ◽

Holothuria Scabra ◽

Research Site ◽

Holothuria Atra

This research is conducted in the coastal waters of Bahoi Village, West Likupang Sub-district, North Minahasa District with the aims to know the types of sea-related environmental factors in the research site and know the community structure of the sea cucumber including the density, Index of diversity, dominance index, and distribution patterns. Data collection was done by using the line transect method, and sampling technique by using quadrat. The results of the study found 5 species of sea cucumber i.e. Holothuria atra, Holothuria scabra, Bohadschia marmorata, Actinopyga echinites, and Bohadschia vitiensis. The individual density of all species is 0.12 ind/m2, and the diversity index (H ') 0.950. The value of the similarity index/compatibility obtained is e = 0.918. The result of the calculated dominance index of sea cucumber species is 1.0 and the pattern of spreading species of sea cucumber in the research site shows a random spread pattern because the value of dispersion index (I) approaches 1 (one). Keywords: community; Sea cucumber; Bahoi; Dominance; Diversity.AbstrakPenelitian ini dilakukan di perairan pantai desa Bahoi, Kecamatan Likupang Barat, Kabupaten Minahasa Utara dengan tujuan untuk mengetahui jenis-jenis teripang yang ada di lokasi penelitian dan mengetahui struktur komunitas teripang yang meliputi kepadatan, indeks keanekaragaman, indeks dominasi, dan pola penyebaran. Pengambilan data dilakukan dengan metode line transek, dan teknik pengambilan sampel dengan mengunakan kuadrat. Hasil penelitian ditemukan 5 spesies teripang yaitu Holothuria atra, Holothuria scabra, Bohadschia marmorata, Actinopyga echinites dan Bohadschia vitiensis. Kepadatan individu dari seluruh spesies yaitu 0,12 ind/m2, indeks keanekaragaman (H’) 0,950. Nilai indeks kemerataan/keserasian diperoleh nilai indeks e = 0,918. nilai indeks dominasi spesies teripang diperoleh 1,0 dan pola penyebaran spesies teripang dilokasi penelitian menunjukkan pola sebaran acak (random) dikarenakan nilai indeks dispersi (I) mendekati 1 (satu).Kata Kunci : Komunitas; Teripang; Perairan Bahoi; Dominasi; Keanekaragaman

Download Full-text

Black sea cucumber (Holothuria atra Jaeger, 1833) rescues Pseudomonas aeruginosa-infected Caenorhabditis elegans via reduction of pathogen virulence factors and enhancement of host immunity

Food & Function ◽

10.1039/c9fo01357a ◽

2019 ◽

Vol 10 (9) ◽

pp. 5759-5767

Author(s):

Wan-Ting Lee ◽

Boon-Khai Tan ◽

Su-Anne Eng ◽

Gan Chee Yuen ◽

Kit Lam Chan ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Virulence Factors ◽

Black Sea ◽

Sea Cucumber ◽

Multidrug Resistant ◽

Host Immunity ◽

Bacterial Virulence ◽

Pathogen Virulence ◽

Multidrug Resistant Pathogens ◽

Holothuria Atra

A strategy to circumvent the problem of multidrug resistant pathogens is the discovery of anti-infectives targeting bacterial virulence or host immunity.

Download Full-text

Colochiroside E, an Unusual Non-holostane Triterpene Sulfated Trioside from the Sea Cucumber Colochirus Robustus and Evidence of the Impossibility of a 7(8)-Double Bond Migration in Lanostane Derivatives having an 18(16)-Lactone

Natural Product Communications ◽

10.1177/1934578x1601100611 ◽

2016 ◽

Vol 11 (6) ◽

pp. 1934578X1601100 ◽

Cited By ~ 1

Author(s):

Alexandra S. Silchenko ◽

Anatoly L Kalinovsky ◽

Sergey A. Avilov ◽

Pelageya V. Andryjaschenko ◽

Pavel S. Dmitrenok ◽

...

Keyword(s):

Double Bond ◽

Triterpene Glycoside ◽

Sea Cucumber ◽

Carbohydrate Chain ◽

2D Nmr ◽

Double Bond Migration ◽

Chlorine Derivative ◽

Rare Group

The unusual non-holostane triterpene glycoside, colochiroside E (1) was isolated from the sea cucumber Colochirus robustus (Cucumariidae, Dendrochirotida). The structure of 1 was established by analysis of ID, 2D NMR and HRESI MS data. Colochiroside E (1) belongs to a rare group of glycosylated 9β-H-lanosta-18(16)-lactones and has an unprecedented sulfated trisaccharide carbohydrate chain consisting of two glucose and one xylose units. In contrast with (9β-H)-7(8)-unsaturated holostane glycosides, the 7(8)-double bond in the having (9β-H)-configuration aglycone of colochiroside E is not capable of migration into the 8(9)- and then into the 9(11)-position on treatment with HC1. The formation of a chlorine derivative of 1 was observed under these conditions.

Download Full-text

Article

Canadian Journal of Chemistry ◽

10.1139/v97-218 ◽

1998 ◽

Vol 76 (2) ◽

pp. 137-141

Author(s):

Sergey A Avilov ◽

Olga A Drozdova ◽

Vladimir I Kalinin ◽

Anatoly I Kalinovsky ◽

Valentin A Stonik ◽

...

Keyword(s):

Antitumor Activity ◽

Spectral Data ◽

Triterpene Glycoside ◽

Sea Cucumber ◽

Sea Cucumbers ◽

Cucumaria Frondosa

Frondoside C (1) is a new sulfated nonholostane triterpene glycoside obtained (with the glycosides closed by aglycone structure as impurities) from the sea cucumber Cucumaria frondosa. Its structure has been elucidated on the basis of spectral data (NMR and MS) of compound 1 and of its desulfated derivative (2) obtained by solvolysis. Frondoside C (1) is just the seventh glycoside reported from sea cucumbers, having a lanostane-type aglycone devoid of the typical 18(20)- lactone ring.Key words: Cucumaria frondosa, sea cucumbers, frondosides, triterepene glycosides, antitumor activity.

Download Full-text

New Cytotoxic Triterpene Glycoside from the East China Sea Cucumber Holothuria nobilis

Natural Product Communications ◽

10.1177/1934578x1501000207 ◽

2015 ◽

Vol 10 (2) ◽

pp. 1934578X1501000

Author(s):

Jia-Jia Zhang ◽

Qi-Ke Zhu ◽

Jun Wu ◽

Hui-Wen Zhang

Keyword(s):

East China Sea ◽

Triterpene Glycoside ◽

Sea Cucumber ◽

Human Tumor ◽

East China ◽

The East China Sea ◽

Human Tumor Cell ◽

Human Tumor Cell Lines ◽

China Sea ◽

Chemical Evidence

A new cytotoxic triterpene glycoside named nobiliside E (1) has been obtained from the East China Sea cucumber Holothuria nobilis Selenka. Its structure was determined on the basis of NMR spectroscopic and MS analyses, together with chemical evidence. The new compound showed significant cytotoxicity to eight human tumor cell lines with IC50 values in the range of 0.53–4.06 μg/mL.

Download Full-text