Pressurized liquid extraction of tectoridin and tectorigenin in Belamcanda chinensis and pressurized water extraction of phyllodulcin in Hydrangea macrophylla

Pressurized water (PW) extraction of galanthamine from Narcissus pseudonarcissus bulbs was performed. The obtained yield was compared with the yield from conventional acidified water extraction and methanolic Soxhlet extraction. Both PW and conventional acidified water extraction were followed by a subsequent purification step for the alkaloids. The PW extraction (70 °C, 150 bar, 45 min) yielded as much galanthamine as methanolic-Soxhlet extraction (ca. 3.50 mg/g). Meanwhile, acid-base extraction with 1% of HBr (v/v) at 65 °C for 3 h gave a lower yield (ca. 2.65 mg/g). A higher PW temperature did not significantly increase the galanthamine yield. Pressure increase is not necessary since more water-soluble compounds such as proteins and polysaccharides are co-extracted, resulting in high viscosity of the water extract solution, which hampers the filtration process. Hence, the acidity of the solution is highly important both in the case of PW extraction and acidified water extraction. Besides galanthamine, the total alkaloid profile following Narcissus alkaloids was also obtained. Lycoramine, O-methyloduline, norgalanthamine, epi-norgalanthamine, narwedine, oduline, haemanthamine, O-methyllycorenine, and a haemanthamine derivate were identified. Although a high yield was obtained from PW extraction, the further purification needs to be improved to obtain an economically feasible industrial extraction process.

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Glycerol as Alternative Co-Solvent for Water Extraction of Polyphenols from Carménère Pomace: Hot Pressurized Liquid Extraction and Computational Chemistry Calculations

Biomolecules ◽

10.3390/biom10030474 ◽

2020 ◽

Vol 10 (3) ◽

pp. 474 ◽

Cited By ~ 3

Author(s):

Nils Leander Huamán-Castilla ◽

María Salomé Mariotti-Celis ◽

Maximiliano Martínez-Cifuentes ◽

José Ricardo Pérez-Correa

Keyword(s):

Hydrogen Bond ◽

Computational Chemistry ◽

Gallic Acid ◽

Liquid Extraction ◽

Grape Pomace ◽

Water Extraction ◽

Pressurized Liquid Extraction ◽

Hydroxyl Group ◽

Solvent Mixtures ◽

Structure Property

Glycerol is a co-solvent for water extraction that has been shown to be highly effective for obtaining polyphenol extracts under atmospheric conditions. However, its efficacy under subcritical conditions has not yet been studied. We assessed different water-glycerol mixtures (15%, 32.5%, and 50%) in a hot pressurized liquid extraction system (HPLE: 10 MPa) at 90 °C, 120 °C, and 150 °C to obtain extracts of low molecular weight polyphenols from Carménère grape pomace. Under the same extraction conditions, glycerol as a co-solvent achieved significantly higher yields in polyphenols than ethanol. Optimal extraction conditions were 150 °C, with 32.5% glycerol for flavonols and 50% for flavanols, stilbenes, and phenolic acids. Considering gallic acid as a model molecule, computational chemistry calculations were applied to explain some unusual extraction outcomes. Furthermore, glycerol, methanol, ethanol, and ethylene glycol were studied to establish an incipient structure–property relationship. The high extraction yields of gallic acid obtained with water and glycerol solvent mixtures can be explained not only by the additional hydrogen bonds between glycerol and gallic acid as compared with the other alcohols, but also because the third hydroxyl group allows the formation of a three-centered hydrogen bond, which intensifies the strongest glycerol and gallic acid hydrogen bond. The above occurs both in neutral and deprotonated gallic acid. Consequently, glycerol confers to the extraction solvent a higher solvation energy of polyphenols than ethanol.

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Pressurized Water Extraction as a Tool for Rapid and Efficient Isolation of Proteins from Almonds

Food Analytical Methods ◽

10.1007/s12161-021-02016-1 ◽

2021 ◽

Author(s):

Lenka Burdějová ◽

Filip Duša ◽

Dana Strouhalová ◽

Dana Moravcová ◽

Pavel Karásek

Keyword(s):

Water Extraction ◽

Pressurized Water ◽

Pressurized Water Extraction

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Pressurized water extraction of isoflavones by experimental design from soybean flour and Soybean Protein Isolate

Food Chemistry ◽

10.1016/j.foodchem.2016.07.053 ◽

2017 ◽

Vol 214 ◽

pp. 9-15 ◽

Cited By ~ 17

Author(s):

Benjamin Moras ◽

Stéphane Rey ◽

Gérard Vilarem ◽

Pierre-Yves Pontalier

Keyword(s):

Experimental Design ◽

Soybean Protein ◽

Protein Isolate ◽

Water Extraction ◽

Soybean Flour ◽

Pressurized Water ◽

Soybean Protein Isolate ◽

Pressurized Water Extraction

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In vitro anti-diabetic effects and phytochemical profiling of novel varieties of Cinnamomum zeylanicum (L.) extracts

PeerJ ◽

10.7717/peerj.10070 ◽

2020 ◽

Vol 8 ◽

pp. e10070

Author(s):

W.A. Niroshani M. Wariyapperuma ◽

Sagarika Kannangara ◽

Yasanandana S. Wijayasinghe ◽

Sri Subramanium ◽

Bimali Jayawardena

Keyword(s):

Microwave Digestion ◽

Alternative Therapy ◽

Water Extraction ◽

Total Phenolic ◽

Cinnamomum Zeylanicum ◽

Pressurized Water ◽

Synthetic Drugs ◽

Inhibitory Potential ◽

Pressurized Water Extraction

Background Diabetes mellitus type 2 (DMT2) is a leading metabolic disorder in the world. Anti-diabetic actions of phytochemicals from various medicinal herbs have been explored as an alternative therapy in the management of DMT2 due to adverse effects of synthetic drugs used in allopathic medicine. α-amylase and α-glucosidase inhibitory potential and phytochemical profiling were investigated in aqueous extracts of two new Cinnamomum zeylanicum accessions, namely C. zeylanicum Sri Wijaya (SW), C. zeylanicum Sri Gemunu (SG) and commercially available C. zeylanicum (CC). Methods Microwave Digestion (MD), Pressurized Water Extraction (PWE), Steam Distillation (SD), Solvent Extraction (SE), Decoction Water Extraction (DWE) and Infusion Water Extraction (IWE) methods were used to prepare Cinnamon quill extracts. Total phenolic content (TPC, Folin-Ciocalteu method) and Proanthocyanidin content (PC, vanillin assay), α-amylase and α-glucosidase inhibition of Cinnamon extracts were determined spectrophotometrically. The α-amylase and α-glucosidase inhibition were reported in terms of IC50 value. The phytochemical profiling was accomplished by GC-MS technique. Results and Discussion Lowest IC50 values were observed in PWE and DWE of SW. The highest PC and TPC were also observed in PWE and DWE of SW. Pressured water and decoctions are promising methods for the extraction of antidiabetic constituents from cinnamon. Benzoic acid, cinnamyl alcohol, benzyl alcohol, and 4-Allyl-2,6-dimethoxyphenol were identified as major compounds in SW extracts. These compounds are believed to be responsible for strong enzyme inhibitory activity of the extracts. Conclusions This is the first study to explore the use of pressured and decoctions water to extract anti-diabetic phytochemicals from cinnamon. The extensive metabolite profiling of novel SW and SG extracts and comparison of that with commercially available CC are reported for the first time in this study. The C. zeylanicum, SW accession holds some promise in the management of diabetes.

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