Topical steroid treatment of allergic rhinitis decreases nasal fluid TH2 cytokines, eosinophils, eosinophil cationic protein, and IgE but has no significant effect on IFN-γ, IL-1β, TNF-α, or neutrophils

2000 ◽  
Vol 106 (2) ◽  
pp. 307-312 ◽  
Author(s):  
Mikael Benson ◽  
Inga-Lisa Strannegård ◽  
Örjan Strannegård ◽  
Göran Wennergren
Keyword(s):  
Allergic Rhinitis ◽  
Eosinophil Cationic Protein ◽  
Topical Steroid ◽  
Steroid Treatment ◽  
Th2 Cytokines ◽  
Cationic Protein ◽  
Tnf Α ◽  
Ifn Γ ◽  
Nasal Fluid

scholarly journals Cytokine Profiles in Nasal Fluid in Patients with Nasal Polyps: A Flow Cytometric Study

2010 ◽  
Vol 29 (1) ◽  
pp. 28-33 ◽  
Author(s):  
Aleksandar Perić ◽  
Danilo Vojvodić ◽  
Vesna Radulović
Keyword(s):  
Nasal Polyps ◽  
Th2 Cytokines ◽  
Eosinophilic Inflammation ◽  
Cytokine Profiles ◽  
Flow Cytometric ◽  
Tnf Α ◽  
Ifn Γ ◽  
Flow Cytometric Study ◽  
Nasal Fluid

Cytokine Profiles in Nasal Fluid in Patients with Nasal Polyps: A Flow Cytometric StudyBiological markers in nasal fluid provide valuable information on nasal pathophysiology. The aims of this study were to compare the cytokine profiles of nasal fluid in subjects with nasal polyps (NP) and co-morbid asthma and NP patients without asthma and to determine the role of these cytokines in the development of NP. Thirty patients with NP (15 asthmatic and 15 non asthmatic) were included in this prospective study. Nasal secretion samples were collected from nasal cavities of all 30 subjects. The levels of eleven cytokines (TNF-α, TNF-β, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, and IFN-γ) were measured using flow cytometry. The concentrations of Th2 cytokines IL-5, IL-6 and IL-10 were significantly higher in patients with NP and asthma compared with subjects with NP without asthma. We also found significantly higher levels of IFN-α, IL-4, IL-6 and IL-10 in allergic patients with NP and asthma compared with those without asthma. In nonallergic patients with NP and asthma, the concentrations of TNF-α, IL-5 and IL-6 were significantly higher than in nonallergic patients with NP without asthma. Our results show that the presence of Th2 cytokines, especially IL-5 and IL-6 in patients with NP and asthma is a more prominent feature than in those without asthma that relates to the increased eosinophilic inflammation. We have also found a significant influence of allergy on the cytokine profiles both in asthmatic and nonasthmatic patients.

Paracoccidioidomycosis: characterization of subpopulations of macrophages and cytokines in human mucosal lesions

2018 ◽  
Vol 57 (6) ◽  
pp. 757-763 ◽  
Author(s):  
C Pagliari ◽  
L Kanashiro-Galo ◽  
A C C Jesus ◽  
M G Saldanha ◽  
M N Sotto
Keyword(s):  
Th2 Cytokines ◽  
Arginase 1 ◽  
Immunohistochemistry Analysis ◽  
Tnf Α ◽  
Ifn Γ ◽  
Mucosal Lesions ◽  
Th1 Cytokines ◽  
Necrosis Factor ◽  
Number Of Cells

AbstractMucosal lesions of paracoccidioidomycosis (PCM) are frequently described and clinically important. Macrophages are classified as M1 or M2. M1 are proinflammatory and M2 are related to chronicity. Dectin-1 recognizes β-glucan and plays an important role against fungal cells. The objective was to verify the presence of M1, M2, and dectin-1 and a possible correlation with Th1/Th2 cytokines in mucosal PCM lesions. In sum, 33 biopsies of oral PCM were submitted to histological and immunohistochemistry analysis, and positive cells were quantified. Eleven biopsies were characterized by compact granulomas (G1), 12 with loose granulomas (G2), and 10 with both kind of granulomas (G3). pSTAT-1 was equally increased in the three groups. G1 was characterized by an increased number of CD163+ macrophages. G2 presented similar number of arginase 1, iNOS, and CD163 expressing cells. G3 presented an increased number of cells expressing arginase 1 and CD163 over iNOS. G1 and G3 presented high number of cells expressing interferon (IFN)-γ; interleukin (IL) 5 was increased in G2 and G3; the expression of IL10 was similar among the three groups, and the expression of tumor necrosis factor (TNF)-α was higher in G3. G1 correlates to Th1 cytokines and pSTAT-1 and G2 correlates to Th2 cytokines. G3 presents both kinds of cytokines. We could not associate the expression of arginase-1, CD163, iNOS, and dectin-1 with the pattern of cytokines or kind of granuloma.

scholarly journals Traditional Chinese MedicineZHENGIdentification Provides a Novel Stratification Approach in Patients with Allergic Rhinitis

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Kai-Li Liang ◽  
Rong-San Jiang ◽  
Chia-Lin Lee ◽  
Pei-Jung Chiang ◽  
Jui-Shan Lin ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Immunoglobulin E ◽  
Linear Regression Analysis ◽  
Eosinophil Cationic Protein ◽  
Multiple Linear Regression Analysis ◽  
Total Serum ◽  
Blood Eosinophil ◽  
Cationic Protein ◽  
Explanatory Factors ◽  
Severity Scores

Background. We aimed to apply theZHENGidentification to provide an easy and useful tool to stratify the patients with allergic rhinitis (AR) through exploring the correlation between the quantified scores of AR symptoms and the TCMZHENGs.Methods. A total of 114 AR patients were enrolled in this observational study. All participants received the examinations of anterior rhinoscopy and acoustic rhinometry. Their blood samples were collected for measurement of total serum immunoglobulin E (IgE), blood eosinophil count (Eos), and serum eosinophil cationic protein (ECP). They also received two questionnaire to assess the severity scores of AR symptoms and quantified TCMZHENGscores. Multiple linear regression analysis was used to determine explanatory factors for the score of AR manifestations.Results. IgE and ECP level, duration of AR, the 2 derived TCMZHENGscores of “Yin-Xu − Yang-Xu”, and “Qi-Xu+Blood-Xu” were 5 explanatory variables to predict the severity scores of AR symptoms. The patients who had higher scores of “Yin-Xu − Yang-Xu” or “Qi-Xu+Blood-Xu” tended to manifest as “sneezer and runner” or “blockers,” respectively.Conclusions.The TCMZHENGscores correlated with the severity scores of AR symptoms and provided an easy and useful tool to stratify the AR patients.

The effect of immunotherapy on the serum levels of eosinophil cationic protein in seasonal allergic rhinitis

1997 ◽  
Vol 22 (2) ◽  
pp. 100-105 ◽  
Author(s):  
Y. OHASHI ◽  
Y. NAKAI ◽  
Y. KAKINOKI ◽  
Y. OHNO ◽  
H. OKAMOTO ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Seasonal Allergic Rhinitis ◽  
Eosinophil Cationic Protein ◽  
Serum Levels ◽  
Cationic Protein

scholarly journals Differentiating the endotypes in allergic rhinitis

2021 ◽  
Vol 36 (2) ◽  
pp. 92-97
Author(s):  
A. V. Klimov ◽  
Z. V. Salahutdinova ◽  
N. A. Pronina ◽  
G. A. Kuznetsov
Keyword(s):  
Allergic Rhinitis ◽  
Eosinophil Cationic Protein ◽  
Skin Tests ◽  
Research Trend ◽  
General Group ◽  
Cationic Protein ◽  
Total Ige ◽  
Interleukin 5 ◽  
Local Allergic Rhinitis ◽  
History Of

Aim. The aim of the study was to differentiate the endotypes in allergic rhinitis by key allergy markers in a mixed group of patients.Material and Methods. The study comprised a total of 48 patients, men and women, aged 18-60 years suffering from three endotypes of allergic rhinitis including the classic, local, and dual allergic rhinitis. The standard diagnostics of allergic rhinitis included taking a history of allergies, family history of allergic disease, video rhinoscopy, serum total IgE level assessment, allergy skin tests to house dust mite and pollen allergens, and study of eosinophilic inflammation parameters (eosinophil cationic protein, interleukin-5 (IL5), and eosinophil counts in blood and nasal secretion).Results. Based on total IgE level, the general group of patients was divided to two subgroups: subgroup 1 comprised patients with high IgE level (n = 22); subgroup 2 comprised patients with low IgE level (n = 26). Most of patients in these groups had contradictory results of allergy skin tests i.e. positive allergy skin test results in case of high IgE level (group 1) and vice versa. Cluster analysis-based exminations of general group allowed to categorize three subgroups of patients: patients with classic allergic rhinitis (n = 22), local allergic rhinitis (n = 22), and dual allergic rhinitis (n = 4). Besides, an increased rate of anxiety disorder was found in patients with local allergic rhinitis (p < 0.001).Conclusion. The obtained data showed promise for a new research trend in studying allergic rhinitis endotypes, namely: investigation of neuroimmune relationships in allergic tolerance disruption in the presence of this pathology.

scholarly journals Macrophage immunomodulatory activity of Acanthopanax senticousus polysaccharide nanoemulsion via activation of P65/JNK/ikkαsignaling pathway and regulation of Th1/Th2 Cytokines

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12575
Author(s):  
Xianghui Li ◽  
Zhiqiang Zhang ◽  
Zhenhuan Guo ◽  
Li Zhao ◽  
Yonglu Liu ◽  
...  
Keyword(s):  
Immune Responses ◽  
Negative Charge ◽  
Bioactive Compound ◽  
Immunomodulatory Activity ◽  
Th2 Cytokines ◽  
Splenocyte Proliferation ◽  
Immunostimulatory Activity ◽  
Tnf Α ◽  
Ifn Γ ◽  
Immune Enhancement

Nanoemulsions (NE) are used widely in pharmaceutical drug formulations and vaccine preparation, and Acanthopanax senticousus polysaccharide (ASPS) is a natural bioactive compound with immunostimulatory activity. Therefore, NE-loaded ASPS is expected to provide immunological enhancement for effective treatment. In the present study, Acanthopanax senticousus polysaccharide (ASPS was encapsulated into nanoemulsions, the resultant ASPS–NE were coated with a negative charge, and the immune enhancement mechanism of these ASPS-NE formulations was analyzed. The immunosuppressive animal models (70 ICR mice, male) for the study were established using cyclophosphamide. In addition, the activation of splenocyte proliferation, phagocytosis of the macrophages, the ratio of CD4+ to CD8+, the concentrations of the cytokines in serum, Western blot analysis was used for the analysis of the P65/JNK/ikk α signaling pathway in the peritoneal macrophage s. The results revealed that the ASPS-NE could stimulated the proliferation of splenocytes and enhance immunity. The ASPS-NE induced the expression of different cytokines (TNF-α, IFN-γ, IL-2, and IL-6), could activate the expressions of P65, JNK, and ikkα, and regulated the Th1/Th2 cytokines. These findings demonstrated the potential of ASPS-NE formulations for drug delivery and to induce potent and sustained immune responses.

scholarly journals Evidence for eosinophil and IL-17 mediated inflammation in allergic rhinitis

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Kawa Amin ◽  
Sulaf Mosa Issa ◽  
Kosar Mohammad Ali ◽  
Muaid Ismiel Aziz ◽  
Huner Mohamed Hama Amieen ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Eosinophil Cationic Protein ◽  
Treatment Strategies ◽  
Serum Levels ◽  
Elisa Test ◽  
Control Group ◽  
Healthy Controls ◽  
Cationic Protein ◽  
Significant Difference ◽  
Symptom Scores

Abstract Background The aim was to determine the level of inflammatory cytokines, eosinophil cationic protein and IgE in allergic rhinitis (AR) patients. Subjects and methods Blood samples were taken from 88 AR patients and 88 healthy controls (HC). Each sample was analysed for eosinophil counts by flow cytometry, IgE by ECLIA, ECP, IL-17, and IL-33 by using ELISA test. Results There was no significant difference between AR patients and the control group in age and gender. Levels of eosinophils, IgE, ECP, IL-17, IL-33 and the total symptom scores were significantly higher in AR patients than the HC (P = 0.0001). Serum ECP correlated with IL-17 (P = 0.041, r = 0.42), IL-33 (P = 0.0001, r = 080), and IgE levels (P = 0.017, r = 0.45) in the R patients. There was no correlation between IL-17 and IL-33. There was a correlation between symptom scores and eosinophils (P = 0.026, r = 0.52), and IgE (P = 0.001, r = 0.60) in the patients. No correlation was observed between symptom scores and ECP, IL-17, and IL-33 in the AR patient. Conclusions Patients with AR have significant higher serum levels of ECP, IL-17, and IL-33 than healthy controls. This indicates that these markers could be used to in order to diagnose AR and to monitor disease. Inhibitory molecules to IL-17 and IL-33 may be considered as novel treatment strategies.

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