Measurement of tumor necrosis factor-α messenger RNA in synovial fibroblasts by real-time quantitative reverse transcriptase–polymerase chain reaction

2001 ◽  
Vol 137 (2) ◽  
pp. 101-106 ◽  
Author(s):  
Minako Takahashi ◽  
Tadao Funato ◽  
Keiko Kumura Ishii ◽  
Mitsuo Kaku ◽  
Takeshi Sasaki
Keyword(s):  
Polymerase Chain Reaction ◽  
Tumor Necrosis Factor ◽  
Messenger Rna ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Synovial Fibroblasts ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Factor Α ◽  
Necrosis Factor

scholarly journals Detection of Tumor Necrosis Factor-α mRNA Induction in Ischemic Brain Tolerance by Means of Real-Time Polymerase Chain Reaction

2000 ◽  
Vol 20 (1) ◽  
pp. 15-20 ◽  
Author(s):  
Xinkang Wang ◽  
Xiang Li ◽  
Joseph A. Erhardt ◽  
Frank C. Barone ◽  
Giora Z. Feuerstein
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Ischemic Preconditioning ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Chain Reaction ◽  
Tnf Α ◽  
Polymerase Chain ◽  
Factor Α ◽  
Necrosis Factor

A short duration of ischemia (i.e., ischemic preconditioning) results in significant brain protection to subsequent severe ischemic insult. Because previous studies suggest that tumor necrosis factor-α (TNF-α) plays a role in both promoting ischemic damage and neuroprotection, the present work aimed to evaluate the expression of TNF-α mRNA in an established model of ischemic preconditioning using a transient 10-minute occlusion of the middle cerebral artery. Because the level of TNF-α mRNA expression in the brain was too low to be consistently detected by Northern technique, a real-time polymerase chain reaction method was applied to quantitate the absolute copy number of TNF-α transcript in rat brain after the preconditioning procedure. TNF-α mRNA was induced in the ipsilateral cortex as early as 1 hour (27 ± 1 copies of mRNA per microgram of tissue compared to 11 ± 3 copies in sham-operated samples) after preconditioning, reached a peak level at 6 hours (49 ± 10 copies of transcript, n = 4, P < 0.01), and persisted up to 2 days. These data not only demonstrate the utility of real-time polymerase chain reaction for sensitive and accurate measurement of mRNA expression in normal and injured tissues but also suggest a potential role of TNF-α in the phenomenon of ischemic preconditioning.

Myostatin induces tumor necrosis factor‐α expression in rheumatoid arthritis synovial fibroblasts through the PI3K–Akt signaling pathway

2018 ◽  
Vol 234 (6) ◽  
pp. 9793-9801 ◽  
Author(s):  
Chen‐Ming Su ◽  
Sung‐Lin Hu ◽  
Yi Sun ◽  
Jin Zhao ◽  
Chengqian Dai ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Tumor Necrosis Factor ◽  
Signaling Pathway ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Synovial Fibroblasts ◽  
Akt Signaling ◽  
Akt Signaling Pathway ◽  
Factor Α ◽  
Necrosis Factor

Tumor necrosis factor α promotes the expression of stem cell factor in synovial fibroblasts and their capacity to induce mast cell chemotaxis

2000 ◽  
Vol 43 (1) ◽  
pp. 164-174 ◽  
Author(s):  
Hans P. Kiener ◽  
Roland Hofbauer ◽  
Makiyeh Tohidast-Akrad ◽  
Sabine Walchshofer ◽  
Kurt Redlich ◽  
...  
Keyword(s):  
Mast Cell ◽  
Stem Cell ◽  
Tumor Necrosis Factor ◽  
Stem Cell Factor ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Synovial Fibroblasts ◽  
Factor Α ◽  
Necrosis Factor ◽  
Cell Chemotaxis

Butyrate suppresses tumor necrosis factor α production by regulating specific messenger RNA degradation mediated through acis-acting AU-rich element

2005 ◽  
Vol 52 (9) ◽  
pp. 2697-2707 ◽  
Author(s):  
Jun Fukae ◽  
Yoshiharu Amasaki ◽  
Yumi Yamashita ◽  
Toshiyuki Bohgaki ◽  
Shinsuke Yasuda ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Messenger Rna ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Rna Degradation ◽  
Factor Α ◽  
Necrosis Factor
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