New sources of resistance to Sclerotinia stem rot caused bySclerotinia sclerotiorumin Chinese and AustralianBrassica napusandB. junceagermplasm screened under Western Australian conditions

2009 ◽  
Vol 38 (2) ◽  
pp. 149 ◽  
Author(s):  
C. X. Li ◽  
S. Y. Liu ◽  
K. Sivasithamparam ◽  
M. J. Barbetti
Keyword(s):  
Stem Rot ◽  
Sclerotinia Stem Rot ◽  
Sources Of Resistance ◽  
Western Australian

Expression of field resistance under Western Australian conditions to Sclerotinia sclerotiorum in Chinese and Australian Brassica napus and Brassica juncea germplasm and its relation with stem diameter

2006 ◽  
Vol 57 (10) ◽  
pp. 1131 ◽  
Author(s):  
C. X. Li ◽  
Hua Li ◽  
K. Sivasithamparam ◽  
T. D. Fu ◽  
Y. C. Li ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Brassica Juncea ◽  
Sclerotinia Sclerotiorum ◽  
Stem Diameter ◽  
Stem Rot ◽  
Lesion Length ◽  
Sclerotinia Stem Rot ◽  
Sources Of Resistance ◽  
Stem Lesion ◽  
Western Australian

Sclerotinia stem rot, caused by Sclerotinia sclerotiorum, has become one of the most serious disease problems in oilseed rape-growing areas in Australia. Sources of resistance to this disease have been sought worldwide. In this study, germplasm comprising 42 Brassica napus and 12 Brassica juncea accessions from China and Australia, was screened for resistance to Sclerotinia stem rot under Western Australian field conditions. Resistance was confirmed in some germplasm from China and new sources of resistance were identified in germplasm from Australia. Furthermore, our study found that the severity of stem lesions was related to stem diameter and percentage of the host plants that were dead. It was evident that both stem lesion length and percentage of plant death were at the lowest level when the stem diameter was approximately 10 mm. Smaller or greater stem diameter resulted both in increased stem lesion length and plant death. Stem diameter may be a useful parameter in breeding cultivars of oilseed Brassicas with Sclerotinia resistance.

The importance of the type and time of inoculation and assessment in the determination of resistance in Brassia napus and B. juncea to Sclerotinia sclerotiorum

2007 ◽  
Vol 58 (12) ◽  
pp. 1198 ◽  
Author(s):  
C. X. Li ◽  
Hua Li ◽  
A. B. Siddique ◽  
K. Sivasithamparam ◽  
P. Salisbury ◽  
...  
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Screening Methods ◽  
Post Inoculation ◽  
Sclerotinia Stem Rot ◽  
Sources Of Resistance ◽  
Myceliogenic Germination ◽  
Variable Impact ◽  
Western Australian ◽  
Mycelial Suspension

Sclerotinia stem rot (SSR) is a significant agricultural problem worldwide. Finding sources of resistance is crucial to the ongoing search for better management of this disease. Brassica germplasm from Australia, China and India was screened for resistance to SSR under Western Australian field conditions following stem inoculation, application of a spray of mycelial suspension, or as a consequence of myceliogenic germination originating from sclerotia resident in soil. Significant differences in response were observed among 53 genotypes using each of the three screening methods. There was a variable impact of the time of inoculation on the disease level depending upon time of assessment post-stem inoculation. However, this impact could be reduced to an insignificant level provided the assessment after stem inoculation was delayed until 3 weeks post-inoculation. The results of these studies indicate that the use of appropriate inoculation and assessment methods could significantly reduce variability in the responses commonly observed in screening for resistance in crop plants against Sclerotinia sclerotiorum.

Identification of new sources of resistance in Rajmash (Phaseolus vulgaris L.) against powdery mildew (Erysiphe polygoni) and stem rot (Sclerotinia sclerotiorum) diseases

2019 ◽  
Author(s):  
R. K. Mishra ◽  
A. K. Parihar ◽  
T. Basvaraj ◽  
Krishna Kumar
Keyword(s):  
Phaseolus Vulgaris ◽  
Powdery Mildew ◽  
Central India ◽  
Stem Rot ◽  
Sclerotinia Stem Rot ◽  
Phaseolus Vulgaris L ◽  
Sources Of Resistance ◽  
Potential Yield ◽  
Powdery Mildew Disease ◽  
Rot Disease

Rajmash (Phaseolus vulgaris L.) is one of the important legume crops of North and Central India. This crop is commonly affected by most devastating diseases like powdery mildew and stem rot. Therefore, identification of new resistance source is urgently needed to achieve potential yield. In present investigation fifty-two genotypes of rajmash comprising landraces, cultivars and exotic genotypes were screened against powdery mildew and stem rot diseases under natural field conditions during rabi 2014-15 and 2015-16. The results revealed that, out of 52 genotypes screened against powdery mildew disease, four genotypes/cultivars were free from powdery mildew disease, 16 were found resistant, 12 were moderately resistant, 4 were moderately susceptible, 10 were susceptible and 6 lines were highly susceptible. In case of Sclerotinia stem rot disease, out of 52 genotypes 20 genotypes exhibited resistance, 12 moderately resistance, 7 found susceptible and 5 genotypes were highly susceptible. Furthermore, only four genotypes viz., EC150250, BLF101, EC 565673A and GPR 203 demonstrated resistance reaction against both powdery mildew and stem rot disease. Thus, these genotypes could be used in breeding programme as donor for development of disease resistant varieties against powdery mildew and stem rot disease in future.

scholarly journals Modeling first order additive × additive epistasis improves accuracy of genomic prediction for sclerotinia stem rot resistance in canola

2021 ◽  
Author(s):  
Mark C Derbyshire ◽  
Yuphin Khentry ◽  
Anita Severn‐Ellis ◽  
Virginia Mwape ◽  
Nur Shuhadah Mohd Saad ◽  
...  
Keyword(s):  
Genomic Prediction ◽  
Stem Rot ◽  
Sclerotinia Stem Rot ◽  
First Order

MicroRNA319a regulates plant resistance to Sclerotinia stem rot

2021 ◽  
Author(s):  
Weiguo Dong ◽  
Wenqing Ren ◽  
Xuan Wang ◽  
He Yuke
Keyword(s):  
Plant Resistance ◽  
Host Susceptibility ◽  
Stem Rot ◽  
Sclerotinia Stem Rot ◽  
Sequencing Data ◽  
Mobility Shift ◽  
Expression Levels ◽  
Affected Plant ◽  
Mobility Shift Assay ◽  
Rot Disease

Abstract MicroRNA319a (miR319a) controls cell division arrest in plant leaves by inhibiting the expression of TCP (TEOSINTE BRANCHED 1/CYCLOIDEA/PCF) family genes. However, it is unclear whether miR319a influences infections by necrotrophic pathogens and host susceptibility. In this study, we revealed that miR319a affected plant resistance to stem rot disease of Sclerotinia sclerotiorum. In the plants of Brassica rapa infected with S. sclerotiorum, miR319a levels increased while expression levels of several BraTCP genes significantly decreased compared with those of the uninfected plants. The overexpression of BraMIR319a in B. rapa increased the susceptibility of the plants to S. sclerotiorum and aggravated stem rot disease, whereas the overexpression of BraTCP4-1 promoted the plant resistance. Our RNA-sequencing data revealed a potential relationship between miR319a and pathogen-related WRKY genes. Chromatin immunoprecipitation (ChIP) assay, electrophoretic mobility shift assay (EMSA) and reporter transaction assay showed that BraTCP4-1 was bound to the promoters of WRKY75, WRKY70, and WRKY33 genes and directly activated these pathogen-related genes. Moreover, the expression levels of WRKY75, WRKY70, and WRKY33 in the plants overexpressing BraMIR319a declined significantly whereas those of the plants overexpressing BraTCP4-1 increased significantly. These results suggest that miR319a and its targeted gene BraTCP4 regulate stem rot resistance through pathways of WRKY genes.

Prevalence of sclerotinia stem rot of canola in New South Wales

2003 ◽  
Vol 43 (2) ◽  
pp. 163 ◽  
Author(s):  
T. L. Hind ◽  
G. J. Ash ◽  
G. M. Murray
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
New South ◽  
New South Wales ◽  
Stem Rot ◽  
Sclerotinia Stem Rot ◽  
Mean Values ◽  
Stem Infection ◽  
South Wales

Surveys of petal infestation and stem infection conducted in 1998, 1999 and 2000 indicated that Sclerotinia sclerotiorum poses a threat to the Australian canola industry. Inoculum was present throughout all canola-growing regions of New South Wales and the stem disease was widespread throughout southern New South Wales. Percentage petal infestation increased over the 3 years surveyed with values ranging from 0 to 99.4%. The highest petal infestation values were observed in 2000 (maximum of 99.4%, mean of 82.2%), with lower mean values in 1998 (38.4%) and 1999 (49.6%). Stem infection ranged from 0 to 37.5% and most fields had less than 10% stem infection. Stem rot incidence before harvest did not relate to percentage petal infestation determined during flowering. This indicated that factors other than percentage petal infestation were important in influencing stem rot incidence. While there was no relationship between percentage petal infestation and stem rot incidence, stem infection never occurred without prior petal infestation.

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