Application of DNA markers in animal industries

2006 ◽  
Vol 46 (2) ◽  
pp. 173 ◽  
Author(s):  
J.-T. Jeon ◽  
J.-H. Lee ◽  
K.-S. Kim ◽  
C.-K. Park ◽  
S.-J. Oh
Keyword(s):  
Mitochondrial Dna ◽  
Beef Cattle ◽  
Dna Markers ◽  
Preliminary Data ◽  
Molecular Tools ◽  
Economic Traits ◽  
Korean Cattle ◽  
D Loop ◽  
Porcine Stress Syndrome ◽  
Animal Industry

The current animal industry is both technology-intensive and globalised. Efficient molecular tools, such as DNA markers, are in demand to strengthen competitive power by maximising the improvement of livestock and obtaining the trust of customers by the verification of product origins. This review describes the present techniques applying DNA markers in the animal industry, with a focus on beef cattle and pigs. Preliminary data from an individual traceability assay for Hanwoo (Korean cattle) using 20 microsatellite markers is described. The potential uses of the assay are demonstrated for several key markers of different traits: for the porcine stress syndrome gene using the RYR mutation; for acid meat using the PRKAG3 mutation; for intramuscular fat using the FABP3 mutation and for fixing the Dominant white allele using KIT duplication. In addition, a possible strategy is suggested to discriminate between pig breeds using mutations of KIT, MC1R, ND2 and the 11-bp insertion in the D-loop of mitochondrial DNA. The industrial application of DNA techniques is limited at present, however, it is expected that DNA markers originating from trait genes, especially those of low-heritability and difficult-to-measure traits, may contribute to maximising the improvement of the major economic traits of animals in the future.

Genetic structure of ukrainian large white pigs, estimated using mitochondrial DNA-markers

2016 ◽  
Vol 3 (1) ◽  
pp. 61-65 ◽  
Author(s):  
K. Pocherniayev
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Structure ◽  
Dna Markers ◽  
Aflp Analysis ◽  
Large White ◽  
Maternal Line ◽  
Wild Pigs ◽  
D Loop ◽  
Wild Pig ◽  
Japan And China

Aim. To study the genetic structure of Ukrainian Large White pigs using mitochondrial DNA-markers. Methods. Multiplex PCR-AFLP analysis, remarkable for the investigation of D-loop fragment between posi- tions 15531 and 15959 of the pig mitochondrial genome with one monomorphic 15558W and fi ve polymorphic sites TasI 15580T > C, 15616T > C, 15714T > C, 15758T > C, 15916A > T, was used. The presence or absence of site TasI in the abovementioned positions determines mitochondrial DNA haplotypes, indicated with Latin letters from A to P. Results. Seven mitochondrial DNA haplotypes of different frequency were revealed among Ukrainian Large White pigs. The highest frequency was noted for haplotypes G (0.141), J1 (0.133) and N (0.591), previously described for wild pigs of Belgium, France, Italy (G); wild pigs of Japan and China, Meis- han and Large White breeds (J1) and wild pigs of Japan and China, Berkshire and Large White breeds (N). The frequency for haplotype L (Belgian wild pig and Large White) is 0.022; haplotype A (French wild boar, Mangalica, Duroc) − 0.003; haplotype C (wild pig of France, Belgium, Poland, Israel and Landrace, Welsh breeds) − 0.092. Haplotype B1 (0.019) was revealed in Myrgorod breed, Poltava Meat and Red White Belted breeds, bred on its basis. Conclusions. The haplogroup of mitochondrial DNA of Ukrainian Large White pigs includes seven haplotypes of mitochondrial DNA. Pigs with haplotypes G, J1, N and L may be referred to rep- resentatives of Ukrainian Large White pigs, which did not have introgression of maternal line of other breeds.

scholarly journals Somatic mitochondrial DNA D-loop mutations in meningioma discovered: A preliminary data

2018 ◽  
Vol 0 (0) ◽  
pp. 0
Author(s):  
AbdulAziz Mohamed Yusoff ◽  
SitiZulaikha Nashwa Mohd Khair ◽  
WanSalihah Wan Abdullah ◽  
SitiMuslihah Abd Radzak ◽  
JafriMalin Abdullah
Keyword(s):  
Mitochondrial Dna ◽  
Preliminary Data ◽  
D Loop

scholarly journals Nuclear and mitochondrial DNA markers in traceability of retail beef samples

2010 ◽  
Vol 30 (9) ◽  
pp. 783-786
Author(s):  
Aline S.M. Cesar ◽  
Fernando H. Biase ◽  
Paula Ripamonte ◽  
Albino Luchiari Filho ◽  
Giovana K. Merighe ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Beef Cattle ◽  
Dna Markers ◽  
Bos Taurus ◽  
Meat Products ◽  
Local Market ◽  
Meat Processing ◽  
Animal Products ◽  
Traceability System ◽  
Meat Samples

Several characteristics are important in a traceability system of animal products, such as age at slaughter, breed composition, besides information of the productive chain. In general, the certification agent records information about the animals and the system which it came from, although cannot guarantee that the slaughtering, meat processing and distribution are error proof. Besides, there is a differential price, at least at the international market, based on sex and breed composition of the animals. Genetic markers allow identification of characteristics controlled in the beef cattle traceability program, as sex and breed composition, in order to correctly identify and appraise the final product for the consumer. The hypothesis of this study was that the majority beef samples retailed in the local market originate from female with a great participation of zebu breeds. Therefore, the objective of this work was to characterize retail beef samples with DNA markers that identify cattle sex and breed composition. Within 10 beef shops localized in Pirassununga, SP, Brazil, 61 samples were collected, all were genotyped as harboring Bos taurus mitochondrial DNA and 18 were positive for the Y chromosome amplification (male). For the marker sat1711b-Msp I the frequency of the allele A was 0.278 and for the marker Lhr-Hha I the frequency of the allele T was 0.417. The results of sat1711b-Msp I and Lhr-Hha I allelic frequencies are suggestive that the proportion of indicus genome compared with the taurine genome in the market meat is smaller than the observed in the Nellore breed. The procedure described in this study identified sex and subspecies characteristics of beef meat samples, with potential application in meat products certification in special as an auxiliary tool in beef cattle traceability programs.

scholarly journals DNA marking of the rate of maturation of gonads in female sterlet x beluga (A. ruthenus x H. huso) hybrid under conditions of recirculation systems

2021 ◽  
Vol 937 (3) ◽  
pp. 032038
Author(s):  
P Geraskin ◽  
I Kornienko ◽  
V Grigoriev ◽  
A Kovaleva ◽  
M Mahotkin
Keyword(s):  
Mitochondrial Dna ◽  
Dna Markers ◽  
New Technologies ◽  
Molecular Genetic ◽  
Base Pairs ◽  
Exact Test ◽  
D Loop ◽  
Mitochondrial Dna Haplotype ◽  
Closed Water ◽  
Conducting Research

Abstract To create a theoretical basis for the development of new technologies for the formation of highly productive sturgeon herds, work has begun on conducting research on DNA - markers associated with economically useful traits. At the first stage of the work, polymorphic regions of the mitochondrial DNA (mtDNA) D-loop were investigated in order to search for promising molecular genetic markers associated with the production properties of sturgeon hybrids. Along with point polymorphisms in mtDNA hybrids, variability in the length of the D-loop was observed, as well as the presence of heteroplasmy in length. The length variability of the D-loop is due to the presence of tandem repeating units in multiples of 80 base pairs (bp). Using Fisher’s exact test, it was shown that the proportion of individuals with four tandem repeating units of 80 bp eachsignificantly higher (p = 0.030) in the group of highly productive hybrids. The obtained data suggest that such a trait as the accelerated maturation of female hybrids (A. ruthenus x H. huso) grown in a closed water supply can be associated with the mitochondrial DNA haplotype, in the D-loop of which there are fourrepeating units.

Genetic Variation in Yellowfin Tuna Thunnus Albacares (Bonnaterre, 1788) Along Indian Coast Using Pcr-Rflp Analysis of Mitochondrial Dna D-Loop Region

2012 ◽  
Vol 3 (1) ◽  
pp. 25-30
Author(s):  
Swaraj Priyaranjan Kunal ◽  
◽  
Girish Kumar Girish Kumar ◽  
Maria Rosalia Menezes
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Variation ◽  
Rflp Analysis ◽  
Yellowfin Tuna ◽  
Thunnus Albacares ◽  
Indian Coast ◽  
Loop Region ◽  
Pcr Rflp ◽  
D Loop

Genetic diversity and origin of mitochondrial DNA D-loop region for some domestic goat breeds of China

2009 ◽  
Vol 30 (9) ◽  
pp. 1187-1194
Author(s):  
Rong-Chao HAO
Keyword(s):  
Genetic Diversity ◽  
Mitochondrial Dna ◽  
Domestic Goat ◽  
Loop Region ◽  
D Loop

The genetic diversity of mitochondrial DNA D-loop region of three geographical populations inTrionyx sinensis

2012 ◽  
Vol 36 (1) ◽  
pp. 17 ◽  
Author(s):  
Xue-zhen HUANG ◽  
Guo-ying QIAN ◽  
Cai-yan LI
Keyword(s):  
Genetic Diversity ◽  
Mitochondrial Dna ◽  
Loop Region ◽  
Geographical Populations ◽  
D Loop
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