DNA marking of the rate of maturation of gonads in female sterlet x beluga (A. ruthenus x H. huso) hybrid under conditions of recirculation systems

Abstract To create a theoretical basis for the development of new technologies for the formation of highly productive sturgeon herds, work has begun on conducting research on DNA - markers associated with economically useful traits. At the first stage of the work, polymorphic regions of the mitochondrial DNA (mtDNA) D-loop were investigated in order to search for promising molecular genetic markers associated with the production properties of sturgeon hybrids. Along with point polymorphisms in mtDNA hybrids, variability in the length of the D-loop was observed, as well as the presence of heteroplasmy in length. The length variability of the D-loop is due to the presence of tandem repeating units in multiples of 80 base pairs (bp). Using Fisher’s exact test, it was shown that the proportion of individuals with four tandem repeating units of 80 bp eachsignificantly higher (p = 0.030) in the group of highly productive hybrids. The obtained data suggest that such a trait as the accelerated maturation of female hybrids (A. ruthenus x H. huso) grown in a closed water supply can be associated with the mitochondrial DNA haplotype, in the D-loop of which there are fourrepeating units.

Download Full-text

Molecular Genetic Status of Aleutian Canada Geese From Buldir and the Semidi Islands, Alaska

The Condor ◽

10.1093/condor/102.1.172 ◽

2000 ◽

Vol 102 (1) ◽

pp. 172-180 ◽

Cited By ~ 2

Author(s):

Barbara J. Pierson ◽

John M. Pearce ◽

Sandra L. Talbot ◽

Gerald F. Shields ◽

Kim T. Scribner

Keyword(s):

Mitochondrial Dna ◽

Random Mating ◽

Molecular Genetic ◽

Canada Goose ◽

Canada Geese ◽

Population Bottlenecks ◽

Base Pairs ◽

Population Genetic Differentiation ◽

Contemporary Gene Flow ◽

Mitochondrial Dna Haplotype

Abstract We conducted genetic analyses of Aleutian Canada Geese (Branta canadensis leucopareia) from Buldir Island in the western Aleutians and the Semidi Islands in the eastern portion of their breeding range. We compared data from seven microsatellite DNA loci and 143 base pairs of the control region of mitochondrial DNA from the two populations of Aleutian Canada Geese and another small-bodied subspecies, the Cackling Canada Goose (B. c. minima) which nests in western Alaska. The widely separated island-nesting Aleutian geese were genetically more closely related to each other than to mainland-nesting small-bodied geese. The populations of Aleutian geese were genetically differentiated from one another in terms of mitochondrial DNA haplotype and microsatellite allele frequencies, suggesting limited contemporary gene flow and/or major shifts in gene frequency through genetic drift. The degree of population genetic differentiation suggests that Aleutian Canada Goose populations could be considered separate management units. There was some evidence of population bottlenecks, although we found no significant genetic evidence of non-random mating or inbreeding.

Download Full-text

Genetic Diversities and Historical Dynamics of Native Ethiopian Horse Populations (Equus caballus) Inferred from Mitochondrial DNA Polymorphisms

Genes ◽

10.3390/genes12020155 ◽

2021 ◽

Vol 12 (2) ◽

pp. 155

Author(s):

Kefena Effa ◽

Sonia Rosenbom ◽

Jianlin Han ◽

Tadelle Dessie ◽

Albano Beja-Pereira

Keyword(s):

Genetic Diversity ◽

Mitochondrial Dna ◽

Genetic Differentiation ◽

Sequence Divergence ◽

Dna Polymorphisms ◽

Base Pairs ◽

Feral Horses ◽

Domestic Horses ◽

D Loop ◽

Diversity Estimates

Matrilineal genetic diversity and relationship were investigated among eight morphologically identified native Ethiopian horse populations using polymorphisms in 46 mtDNA D-loop sequences (454 base pairs). The horse populations identified were Abyssinian, Bale, Borana, Horro, Kafa, Kundido feral horses, Ogaden and Selale. Mitochondrial DNA D-loop sequences were characterized by 15 variable sites that defined five different haplotypes. All genetic diversity estimates, including Reynolds’ linearized genetic distance, genetic differentiation (FST) and nucleotide sequence divergence (DA), revealed a low genetic differentiation in native Ethiopian horse populations. However, Kundido feral and Borana domestic horses were slightly diverged from the rest of the Ethiopian horse populations. We also tried to shed some light on the matrilineal genetic root of native Ethiopian horses from a network constructed by combining newly generated haplotypes and reference haplotypes deposited in the GenBank for Eurasian type Turkish Anatolian horses that were used as a genetic conduit between Eurasian and African horse populations. Ninety-two haplotypes were generated from the combined Ethio-Eurasian mtDNA D-loop sequences. A network reconstructed from the combined haplotypes using Median-Joining algorithm showed that haplotypes generated from native Ethiopian horses formed separate clusters. The present result encourages further investigation of the genetic origin of native African horses by retrieving additional mtDNA sequences deposited in the GenBank for African and Eurasian type horses.

Download Full-text

Genetic structure of ukrainian large white pigs, estimated using mitochondrial DNA-markers

Agricultural science and practice ◽

10.15407/agrisp3.01.061 ◽

2016 ◽

Vol 3 (1) ◽

pp. 61-65 ◽

Cited By ~ 1

Author(s):

K. Pocherniayev

Keyword(s):

Mitochondrial Dna ◽

Genetic Structure ◽

Dna Markers ◽

Aflp Analysis ◽

Large White ◽

Maternal Line ◽

Wild Pigs ◽

D Loop ◽

Wild Pig ◽

Japan And China

Aim. To study the genetic structure of Ukrainian Large White pigs using mitochondrial DNA-markers. Methods. Multiplex PCR-AFLP analysis, remarkable for the investigation of D-loop fragment between posi- tions 15531 and 15959 of the pig mitochondrial genome with one monomorphic 15558W and fi ve polymorphic sites TasI 15580T > C, 15616T > C, 15714T > C, 15758T > C, 15916A > T, was used. The presence or absence of site TasI in the abovementioned positions determines mitochondrial DNA haplotypes, indicated with Latin letters from A to P. Results. Seven mitochondrial DNA haplotypes of different frequency were revealed among Ukrainian Large White pigs. The highest frequency was noted for haplotypes G (0.141), J1 (0.133) and N (0.591), previously described for wild pigs of Belgium, France, Italy (G); wild pigs of Japan and China, Meis- han and Large White breeds (J1) and wild pigs of Japan and China, Berkshire and Large White breeds (N). The frequency for haplotype L (Belgian wild pig and Large White) is 0.022; haplotype A (French wild boar, Mangalica, Duroc) − 0.003; haplotype C (wild pig of France, Belgium, Poland, Israel and Landrace, Welsh breeds) − 0.092. Haplotype B1 (0.019) was revealed in Myrgorod breed, Poltava Meat and Red White Belted breeds, bred on its basis. Conclusions. The haplogroup of mitochondrial DNA of Ukrainian Large White pigs includes seven haplotypes of mitochondrial DNA. Pigs with haplotypes G, J1, N and L may be referred to rep- resentatives of Ukrainian Large White pigs, which did not have introgression of maternal line of other breeds.

Download Full-text

Application of DNA markers in animal industries

Australian Journal of Experimental Agriculture ◽

10.1071/ea05191 ◽

2006 ◽

Vol 46 (2) ◽

pp. 173 ◽

Cited By ~ 7

Author(s):

J.-T. Jeon ◽

J.-H. Lee ◽

K.-S. Kim ◽

C.-K. Park ◽

S.-J. Oh

Keyword(s):

Mitochondrial Dna ◽

Beef Cattle ◽

Dna Markers ◽

Preliminary Data ◽

Molecular Tools ◽

Economic Traits ◽

Korean Cattle ◽

D Loop ◽

Porcine Stress Syndrome ◽

Animal Industry

The current animal industry is both technology-intensive and globalised. Efficient molecular tools, such as DNA markers, are in demand to strengthen competitive power by maximising the improvement of livestock and obtaining the trust of customers by the verification of product origins. This review describes the present techniques applying DNA markers in the animal industry, with a focus on beef cattle and pigs. Preliminary data from an individual traceability assay for Hanwoo (Korean cattle) using 20 microsatellite markers is described. The potential uses of the assay are demonstrated for several key markers of different traits: for the porcine stress syndrome gene using the RYR mutation; for acid meat using the PRKAG3 mutation; for intramuscular fat using the FABP3 mutation and for fixing the Dominant white allele using KIT duplication. In addition, a possible strategy is suggested to discriminate between pig breeds using mutations of KIT, MC1R, ND2 and the 11-bp insertion in the D-loop of mitochondrial DNA. The industrial application of DNA techniques is limited at present, however, it is expected that DNA markers originating from trait genes, especially those of low-heritability and difficult-to-measure traits, may contribute to maximising the improvement of the major economic traits of animals in the future.

Download Full-text

Mitochondrial D-loop sequence variation among Hucul horse

Czech Journal of Animal Science ◽

10.17221/6992-cjas ◽

2013 ◽

Vol 58 (No. 10) ◽

pp. 437-442 ◽

Cited By ~ 5

Author(s):

V. Czerneková ◽

T. Kott ◽

I. Majzlík

Keyword(s):

Mitochondrial Dna ◽

Genetic Information ◽

Sequence Variation ◽

Dna Typing ◽

Conservation Strategies ◽

Base Pairs ◽

Horse Population ◽

Loop Region ◽

Loop Sequence ◽

D Loop

Genetic variation in the Czech Hucul horse population was analyzed using a sequence analysis of the D-loop region of mitochondrial DNA. One hundred and sixty-five Hucul horses were tested. Sequencing of the 700-base pairs fragment of the mitochondrial DNA D-loop region revealed 38 mutation sites representing 14 haplotypes, which were clustered into six haplogroups. The genetic information obtained from the mitochondrial DNA typing is of utmost importance for the future breed-conservation strategies.  

Download Full-text

Application of molecular genetic methods for species Equus caballus – Literature review

Acta Agraria Debreceniensis ◽

10.34101/actaagrar/73/1621 ◽

2017 ◽

pp. 21-28

Author(s):

Nikolett Csizmár ◽

Szilvia Kusza

Keyword(s):

Mitochondrial Dna ◽

Literature Review ◽

Molecular Genetic ◽

Equus Caballus ◽

Genetic Studies ◽

Loop Region ◽

D Loop

In this study our aim was to provide a comprihensive overview of the most commonly used methods in molecular genetic studies related to Equus caballus. Thus we are dealing with the D-loop region of mitochondrial DNA, with microsatellites and also with single nucleotid polimorphism as SNP. The advantages and drawbacks of each method were also explored.

Download Full-text

Intraspecific variation in sibling species of Simulium venustum and Simulium verecundum complexes (Diptera: Simuliidae) revealed by the sequence of the mitochondrial 16S rRNAgene

Canadian Journal of Zoology ◽

10.1139/z93-164 ◽

1993 ◽

Vol 71 (6) ◽

pp. 1202-1206 ◽

Cited By ~ 8

Author(s):

Bai Xiong ◽

Thomas D. Kocher

Keyword(s):

Mitochondrial Dna ◽

Intraspecific Variation ◽

New Hampshire ◽

Ribosomal Rna ◽

Sibling Species ◽

Black Flies ◽

Equal Frequency ◽

Base Pairs ◽

Three Samples ◽

Mitochondrial Dna Haplotype

A portion of the mitochondrial large ribosomal RNA gene was amplified and sequenced (460 base pairs) for 10 individuals of each of nine samples of black flies representing three sibling species of the Simulium venustum complex (CC, CC3, and AC(gB)) and two sibling species of the Simulium verecundum complex (ACD = Simulium rostratum and AA). Mitochondrial DNA haplotype heterogeneity was observed in all but the sample of S. verecundum AA from New Hampshire. The sample of S. venustum CC3 had a significantly larger number of haplotypes than other samples. A predominant haplotype was found in most samples except S. venustum CC3 and S. venustum CC from New Hampshire (nine haplotypes in the sample of S. venustum CC3 and two haplotypes with equal frequency in the sample of S. venustum CC from New Hampshire). The predominant haplotype was the same among the three samples of S. verecundum AA. Samples of S. venustum CC also shared haplotypes, but the predominant haplotype was different in each sample. Despite intraspecific variation, haplotypes are usually clustered on a conspecific basis.

Download Full-text