Four AUXIN RESPONSE FACTOR genes downregulated by microRNA167 are associated with growth and development in Oryza sativa

2012 ◽  
Vol 39 (9) ◽  
pp. 736 ◽  
Author(s):  
Hai Liu ◽  
Shenghua Jia ◽  
Defeng Shen ◽  
Jin Liu ◽  
Jie Li ◽  
...  
Keyword(s):  
Transgenic Rice ◽  
Growth And Development ◽  
Target Genes ◽  
High Efficiency ◽  
Developmental Process ◽  
Auxin Response Factor ◽  
Response Factor ◽  
Mrna Levels ◽  
Auxin Response

MicroRNA167 (miR167), as a conserved miRNA, has been implicated in auxin signalling by regulating the expression of certain auxin response factor (ARF) genes to determine the plant developmental process. Among the 10 MIR167 genes of rice, the precursor structures derived from MIR167a, MIR167b and MIR167c produce miR167 with high efficiency. To explore the biological function of miR167 in rice, four of its predicted target genes, OsARF6, OsARF12, OsARF17 and OsARF25, were identified in vivo. Although the expression levels of miR167 and its target OsARFs did not show an obvious negative correlation, the enhanced miR167 level in transgenic rice overexpressing miR167 resulted in a substantial decrease in mRNA levels of the four OsARF genes. Moreover, the transgenic rice plants were small in stature with remarkably reduced tiller number. These results suggest that miR167 is important for the appropriate expression of at least four OsARFs, which mediate the auxin response, to contribute to the normal growth and development of rice.

scholarly journals Antisense Phenotypes Reveal a Functional Expression of OsARF1 , an Auxin Response Factor, in Transgenic Rice

2009 ◽  
Author(s):  
Kotb A. Attia ◽  
Amr F. Abdelkhalik ◽  
Megahed H. Ammar ◽  
Chun Wei ◽  
Jinshui Yang ◽  
...  
Keyword(s):  
Transgenic Rice ◽  
Functional Expression ◽  
Auxin Response Factor ◽  
Response Factor ◽  
Auxin Response

scholarly journals Genome-Wide Identification Analysis of The Auxin Response Factor Gene Family (ARF) in Zizania Latifolia and its Role in Swelling Stem Formation After Ustilago Esculenta Infection

2021 ◽  
Author(s):  
Jie Li ◽  
Jinfeng Hou ◽  
Zhiyuan Lu ◽  
Yang Yang ◽  
Hao Wang ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Gene Family ◽  
Growth And Development ◽  
Auxin Response Factor ◽  
Response Factor ◽  
Rna Seq ◽  
Auxin Response ◽  
Zizania Latifolia ◽  
Factor Gene ◽  
Auxin Response Factor Gene

Abstract Background: Auxin signalling plays a crucial role in plant growth and development. Although the auxin response factor (ARF) gene family has been studied in some plant species, its structural features, molecular evolution, and expression profile in Zizania latifolia (Z. latifolia) are still not clear.Results: Our study identified 33 putative YSL genes from the whole Z. latifolia genome. Furthermore, a comprehensive overview of the ZlARFs was undertaken, including phylogenetic relationship, gene structures, conserved domains, synteny, Ka/Ks, motifs, and subcellular locations of the gene product. Synteny analyses and the calculation of Ka/Ks values suggested that all 57 orthologous/paralogous gene pairs between Z. latifolia and Z. latifolia, Z. latifolia and Oryza sativa have experienced strong purifying selection. The phylogenetic analysis of ARFs indicated that the ZlARFs can be divided into 6 classes and that most ZlARFs from Z. latifolia have closer relationships with Oryza sativa than with Arabidopsis. RNA-Seq data and qRT-PCR analyses showed that ZlARF genes were expressed in TDF treatment and U. esculenta infection, while some ZlARFs exhibited high expression levels only in U. esculenta infection. Meanwhile, the interaction networks and gene ontology (GO) term of the ZlARF genes were constructed and 23 ZlARF co-expressed genes were identified, most of which were down-regulated involve auxin-activated signalling pathway in after swelling stem formation. Transcriptome analysis results verified the relevant functions of ARF genes, and most ZlARF genes regulated physiological processes in response to differential cell expansion. Conclusion: Comprehensive bioinformation analysis of the auxin response factor gene family (ARF) in Z. latifolia and its association with swelling stem formation after U. esculenta infection. The bioinformatic and RNA-Seq analyses provided valuable information for further study on the regulation of the growth and development of swelling stem formation by ZlARFs in Z. latifolia.

scholarly journals Characterization of cotton ARF factors and the role of GhARF2b in fiber development

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Xiufang Zhang ◽  
Junfeng Cao ◽  
Chaochen Huang ◽  
Zishou Zheng ◽  
Xia Liu ◽  
...  
Keyword(s):  
Cotton Fiber ◽  
Target Genes ◽  
Early Stage ◽  
Fiber Development ◽  
Auxin Response Factor ◽  
Response Factor ◽  
Auxin Response ◽  
Cotton Fiber Development ◽  
Cotton Species

AbstractBackgroundCotton fiber is a model system for studying plant cell development. At present, the functions of many transcription factors in cotton fiber development have been elucidated, however, the roles of auxin response factor (ARF) genes in cotton fiber development need be further explored.ResultsHere, we identify auxin response factor (ARF) genes in three cotton species: the tetraploid upland cottonG. hirsutum, which has 73 ARF genes, and its putative extent parental diploidsG. arboreumandG. raimondii, which have 36 and 35 ARFs, respectively. Ka and Ks analyses revealed that inG. hirsutum ARFgenes have undergone asymmetric evolution in the two subgenomes. The cotton ARFs can be classified into four phylogenetic clades and are actively expressed in young tissues. We demonstrate thatGhARF2b, a homolog of the ArabidopsisAtARF2, was preferentially expressed in developing ovules and fibers. Overexpression ofGhARF2bby a fiber specific promoter inhibited fiber cell elongation but promoted initiation and, conversely, its downregulation by RNAi resulted in fewer but longer fiber. We show that GhARF2b directly interacts with GhHOX3 and represses the transcriptional activity of GhHOX3 on target genes.ConclusionOur results uncover an important role of the ARF factor in modulating cotton fiber development at the early stage.

scholarly journals Auxin response factor gene family in Brassica rapa: genomic organization, divergence, expression, and evolution

2012 ◽  
Vol 287 (10) ◽  
pp. 765-784 ◽  
Author(s):  
Jeong-Hwan Mun ◽  
Hee-Ju Yu ◽  
Ja Young Shin ◽  
Mijin Oh ◽  
Hyun-Ju Hwang ◽  
...  
Keyword(s):  
Gene Family ◽  
Brassica Rapa ◽  
Genomic Organization ◽  
Auxin Response Factor ◽  
Response Factor ◽  
Auxin Response ◽  
Factor Gene ◽  
Auxin Response Factor Gene

Genome-wide analysis of the auxin response factor (ARF) gene family in maize (Zea mays)

2010 ◽  
Vol 63 (3) ◽  
pp. 225-234 ◽  
Author(s):  
Yan Liu ◽  
HaiYang Jiang ◽  
Wenjuan Chen ◽  
Yexiong Qian ◽  
Qing Ma ◽  
...  
Keyword(s):  
Zea Mays ◽  
Gene Family ◽  
Auxin Response Factor ◽  
Response Factor ◽  
Auxin Response ◽  
Genome Wide Analysis ◽  
Genome Wide

scholarly journals Circular RNA CDR1as Exerts Oncogenic Properties Partially through Regulating MicroRNA 641 in Cholangiocarcinoma

2020 ◽  
Vol 40 (15) ◽  
Author(s):  
Dingyang Li ◽  
Zhe Tang ◽  
Zhiqiang Gao ◽  
Pengcheng Shen ◽  
Zhaochen Liu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Target Genes ◽  
Circular Rna ◽  
Tumor Xenograft ◽  
Mrna Levels ◽  
Cell Behaviors ◽  
Xenograft Growth ◽  
Tumor Xenograft Growth

ABSTRACT It has been found that the circular RNA (circRNA) CDR1as is upregulated in cholangiocarcinoma (CCA) tissues. In this study, we tried to explore the roles of CDR1as in CCA. CDR1as was overexpressed or knocked down in human CCA cells to assess the effects of CDR1as on cell behaviors and tumor xenograft growth. In vitro, the CDR1as level was significantly increased in CCA cell lines. The results showed that CDR1as promoted the cell proliferation, migration, invasion, and activation of the AKT3/mTOR pathway in CCA cells. Moreover, miR-641, a predicted target microRNA (miRNA) of CDR1as, could partially reverse the effects of CDR1as on cell behaviors in CCA cells. Furthermore, CDR1as improved tumor xenograft growth, and it could be attenuated by miR-641 in vivo. Additionally, CDR1as expression was inversely correlated with miR-641 in CCA cells, and miR-641 could directly bind with CDR1as and its target genes, the AKT3 and mTOR genes. Mechanistically, CDR1as could bind with miR-641 and accelerate miR-641 degradation, which possibly leads to the upregulation of the relative mRNA levels of AKT3 and mTOR in RBE cells. In conclusion, our findings indicated that CDR1as might exert oncogenic properties, at least partially, by regulating miR-641 in CCA. CDR1as and miR-641 could be considered therapeutic targets for CCA.

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