Phenotyping oilseed rape growth-related traits and their responses to water deficit: the disturbing pot size effect

2017 ◽  
Vol 44 (1) ◽  
pp. 35 ◽  
Author(s):  
Anaëlle Dambreville ◽  
Mélanie Griolet ◽  
Gaëlle Rolland ◽  
Myriam Dauzat ◽  
Alexis Bédiée ◽  
...  
Keyword(s):  
Water Deficit ◽  
Oilseed Rape ◽  
High Throughput ◽  
Interactive Effects ◽  
Brassica Napus L ◽  
Root Shoot Ratio ◽  
Whole Plant ◽  
Pot Size ◽  
High Throughput Phenotyping ◽  
Root Restriction

Following the recent development of high-throughput phenotyping platforms for plant research, the number of individual plants grown together in a same experiment has raised, sometimes at the expense of pot size. However, root restriction in excessively small pots affects plant growth and carbon partitioning, and may interact with other stresses targeted in these experiments. In work reported here, we investigated the interactive effects of pot size and soil water deficit on multiple growth-related traits from the cellular to the whole-plant scale in oilseed rape (Brassica napus L.). The effects of pot size on responses to water deficit and allometric relationships revealed strong, multilevel interactions between pot size and watering regime. Notably, water deficit increased the root : shoot ratio in large pots, but not in small pots. At the cellular scale, water deficit decreased epidermal leaf cell area in large pots, but not in small pots. These results were consistent with changes in the level of endoreduplication factor in leaf cells. Our study illustrates the disturbing interaction of pot size with water deficit and raises the need to carefully consider this factor in the frame of the current development of high-throughput phenotyping experiments.

scholarly journals High-throughput phenotyping (HTP) identifies seedling root traits linked to variation in seed yield and nutrient capture in field-grown oilseed rape (Brassica napusL.)

2016 ◽  
Vol 118 (4) ◽  
pp. 655-665 ◽  
Author(s):  
C. L. Thomas ◽  
N. S. Graham ◽  
R. Hayden ◽  
M. C. Meacham ◽  
K. Neugebauer ◽  
...  
Keyword(s):  
Oilseed Rape ◽  
Seed Yield ◽  
High Throughput ◽  
Root Traits ◽  
Seedling Root ◽  
High Throughput Phenotyping

scholarly journals Proteomic data from leaves of twenty-four sunflower genotypes underwater deficit

2020 ◽  
Author(s):  
Thierry Balliau ◽  
Harold Duruflé ◽  
Nicolas Blanchet ◽  
Mélisande Blein-Nicolas ◽  
Nicolas B. Langlade ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Water Deficit ◽  
High Throughput ◽  
Molecular Basis ◽  
Inbred Lines ◽  
Vegetative Stage ◽  
Valuable Resource ◽  
Proteomic Data ◽  
High Throughput Phenotyping

AbstractThis article describes how the proteomic data were produced on sunflower plants subjected to water deficit. Twenty-four sunflower genotypes were selected to represent genetic diversity within cultivated sunflower. They included both inbred lines and their hybridsWater deficit was applied to plants in pots at the vegetative stage using the high-throughput phenotyping platform Heliaphen. Here, we provide proteomic data from sunflower leaves corresponding to the identification of 3062 proteins and the quantification of 1211 of them in these 24 genotypes grown in two watering conditions. These data differentiate both treatment and the different genotypes and constitute a valuable resource to the community to study adaptation of crops to drought and the molecular basis of heterosis.

scholarly journals Phenotyping Root Systems in a Set of Japonica Rice Accessions: Can Structural Traits Predict the Response to Drought?

Rice ◽  
2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Paulo Henrique Ramos Guimarães ◽  
Isabela Pereira de Lima ◽  
Adriano Pereira de Castro ◽  
Anna Cristina Lanna ◽  
Patrícia Guimarães Santos Melo ◽  
...  
Keyword(s):  
Root System ◽  
Water Deficit ◽  
High Throughput ◽  
Root Traits ◽  
Root Systems ◽  
Japonica Rice ◽  
Plastic Response ◽  
Reproductive Phase ◽  
High Throughput Phenotyping ◽  
Structural Traits

Abstract Background The root system plays a major role in plant growth and development and root system architecture is reported to be the main trait related to plant adaptation to drought. However, phenotyping root systems in situ is not suited to high-throughput methods, leading to the development of non-destructive methods for evaluations in more or less controlled root environments. This study used a root phenotyping platform with a panel of 20 japonica rice accessions in order to: (i) assess their genetic diversity for a set of structural and morphological root traits and classify the different types; (ii) analyze the plastic response of their root system to a water deficit at reproductive phase and (iii) explore the ability of the platform for high-throughput phenotyping of root structure and morphology. Results High variability for the studied root traits was found in the reduced set of accessions. Using eight selected traits under irrigated conditions, five root clusters were found that differed in root thickness, branching index and the pattern of fine and thick root distribution along the profile. When water deficit occurred at reproductive phase, some accessions significantly reduced root growth compared to the irrigated treatment, while others stimulated it. It was found that root cluster, as defined under irrigated conditions, could not predict the plastic response of roots under drought. Conclusions This study revealed the possibility of reconstructing the structure of root systems from scanned images. It was thus possible to significantly class root systems according to simple structural traits, opening up the way for using such a platform for medium to high-throughput phenotyping. The study also highlighted the uncoupling between root structures under non-limiting water conditions and their response to drought.

Distribution of 14carbon-labelled assimilates in flowering plants of oilseed rape (Brassica napus L.)

1988 ◽  
Vol 111 (2) ◽  
pp. 347-355 ◽  
Author(s):  
D. R. Keiller ◽  
D. G. Morgan
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Flowering Plants ◽  
Flower Buds ◽  
Brassica Napus L ◽  
Whole Plant ◽  
Sink Capacity ◽  
Days After Anthesis ◽  
Plant Basis

SummaryA study has been made of the distribution of 14carbon-labelled assimilates in flowering oilseed rape plants. Up to 10 days after anthesis individual flowers were strong sinks for assimilates for 2 days after opening but thereafter became weak sinks for a period of 24 h. This period coincided with pollination and subsequent fertilization. After fertilization the young pods again became strong sinks. Between 13 and 16 days after anthesis other flower buds, flowers and young pods which had previously been strong sinks failed to attract assimilates and aborted. This fall in sink capacity occurred at about the same time on all branches and appeared to be co-ordinated on a whole-plant basis.

Warming alters the positive impact of elevated CO2 concentration on cotton growth and physiology during soil water deficit

2017 ◽  
Vol 44 (2) ◽  
pp. 267 ◽  
Author(s):  
Katrina J. Broughton ◽  
Renee A. Smith ◽  
Remko A. Duursma ◽  
Daniel K. Y. Tan ◽  
Paxton Payton ◽  
...  
Keyword(s):  
Soil Water ◽  
Water Deficit ◽  
Elevated Co2 ◽  
Water Loss ◽  
Recovery Period ◽  
Co2 Concentration ◽  
Interactive Effects ◽  
Plant Water ◽  
Soil Water Deficit ◽  
Whole Plant

Alterations in climate factors such as rising CO2 concentration ([CO2]), warming and reduced precipitation may have significant impacts on plant physiology and growth. This research investigated the interactive effects of elevated [CO2], warming and soil water deficit on biomass production, leaf-level physiological responses and whole-plant water use efficiency (WUEP) in cotton (Gossypium hirsutum L.). Cotton was grown in the glasshouse under two [CO2] treatments (CA, 400 µL L–1; CE, 640 µL L–1) and two temperature treatments (TA, 28°C : 17°C day : night; TE, 32°C : 21°C day : night). Plants were subjected to two progressive water deficit cycles, with a 5-day recovery period between the water deficit periods. CE increased vegetative biomass and photosynthetic rates, and decreased stomatal conductance in TA; however, these responses to CE were not evident under TE. CE increased whole-plant water loss under TA, but increased WUEp, whereas increased whole-plant water loss in TE decreased WUEp regardless of atmospheric [CO2]. CE may provide some positive growth and physiological benefits to cotton at TA if sufficient water is available but CE will not mitigate the negative effects of rising temperature on cotton growth and physiology in future environments.

Oilseed rape (Brassica napus L.) pod shatter resistance and its relationship with whole plant and pod characteristics

2021 ◽  
Vol 166 ◽  
pp. 113459
Author(s):  
Yiren Qing ◽  
Yaoming Li ◽  
Lizhang Xu ◽  
Zheng Ma ◽  
Xiaoli Tan ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Brassica Napus L ◽  
Whole Plant ◽  
Pod Shatter

A functional leaf may represent the assimilate accumulation characteristics of the whole seedling plant in winter oilseed rape (Brassica napus L.)

2015 ◽  
Vol 66 (8) ◽  
pp. 849 ◽  
Author(s):  
Xinghua Li ◽  
Tewu Yang ◽  
Zhongnan Nie ◽  
Guoxing Chen ◽  
Liyong Hu ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Dry Matter ◽  
Seedling Stage ◽  
Regression Equations ◽  
Winter Oilseed Rape ◽  
Brassica Napus L ◽  
Whole Plant ◽  
Photosynthetic Assimilation ◽  
Accumulation Characteristics

A single functional leaf is usually sampled to evaluate the growth and photosynthetic assimilation of crops. However, there is large variation between leaves in winter oilseed rape (Brassica napus L.) at seedling stage. In this study, the morphological and physiological characteristics of various functional leaves were compared with characteristics of the whole plant at seedling stage for 2 years by using the oilseed rape cultivars Huaza 9 and Huaza 62 as plant material. The aim of this study was to identify a leaf that can represent the whole plant for assimilate accumulation characteristics at the seedling stage of the crop. The results showed that the photosynthetic rate and the contents of non-structural carbohydrates, nutrients and soluble proteins in fourth and fifth leaf of a plant were most closely related to those of the whole plant. The area and dry matter (DM) of all functional leaves were well correlated with those of whole plant, with the fifth leaf having the highest correlations. It is therefore recommended that the fifth leaf is most suitable to represent the whole plant for evaluation of growth and assimilate accumulation for winter oilseed rape at the seedling stage. The following regression equations for whole plant (y) and fifth leaf (x, dry matter or length × width) can be used to predict (1) DM accumulation (g) and (2) total leaf area (cm2) of the whole plant: (1) y = 3.32x + 1.51 (R2 = 0.88, P < 0.001); (2) y = 1.24x + 222.69 (R2 = 0.67, P < 0.001).

scholarly journals Proteomic data from leaves of twenty-four sunflower genotypes under water deficit

OCL ◽  
2021 ◽  
Vol 28 ◽  
pp. 12
Author(s):  
Thierry Balliau ◽  
Harold Duruflé ◽  
Nicolas Blanchet ◽  
Mélisande Blein-Nicolas ◽  
Nicolas B. Langlade ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Water Deficit ◽  
High Throughput ◽  
Molecular Basis ◽  
Inbred Lines ◽  
Vegetative Stage ◽  
Valuable Resource ◽  
Data Set ◽  
Proteomic Data ◽  
High Throughput Phenotyping

This article describes a proteomic data set produced from sunflower plants subjected to water deficit. Twenty-four sunflower genotypes were selected to represent genetic diversity within cultivated sunflower. They included both inbred lines and their hybrids. Water deficit was applied to plants in pots at the vegetative stage using the high-throughput phenotyping platform Heliaphen. We present here the identification of 3062 proteins and the quantification of 1211 of them in the leaves of the 24 genotypes grown under two watering conditions. These data allow the study of both the effects of genetic variations and watering conditions. They constitute a valuable resource for the community to study adaptation of crops to drought and the molecular basis of heterosis.

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