Hydrogen-rich water promotes elongation of hypocotyls and roots in plants through mediating the level of endogenous gibberellin and auxin

2020 ◽  
Vol 47 (9) ◽  
pp. 771
Author(s):  
Qi Wu ◽  
Nana Su ◽  
Xin Huang ◽  
Xiaoping Ling ◽  
Min Yu ◽  
...  
Keyword(s):  
Mung Bean ◽  
Hypocotyl Elongation ◽  
General Nature ◽  
Hypocotyl Length ◽  
Root Cells ◽  
Protected Cultivation ◽  
Endogenous Phytohormones ◽  
Growth Promoting ◽  
Radish Seedlings ◽  
Growth Of Seedlings

The aim of this study was to investigate effects of the hydrogen-rich water (HRW) on the vegetable growth, and explore the possibility of applying HRW for protected cultivation of vegetables. Results showed that compared with control, HRW treatment significantly promoted fresh weight, hypocotyl length and root length of mung bean seedlings. The strongest stimulation was observed for 480 μM H2 (60% of saturated HRW concentration) treatment. This concentration was used in the following experiments. The enhanced cell elongation was correlated with the changes in the level of endogenous phytohormones. In the dark-grown hypocotyls and roots of mung bean seedlings, HRW significantly increased the content of IAA and GA3. Addition of GA3 enhanced the hypocotyl elongation only. uniconazole, an inhibitor of GA3 biosynthesis, inhibited HRW-induced hypocotyl elongation, but did not affect root elongation. Exogenous application of IAA promoted HRW effects on elongation of both the hypocotyl and the root, while the IAA biosynthesis inhibitor TIBA negated the above affects. The general nature of HRW-induced growth-promoting effects was further confirmed in experiments involving cucumber and radish seedlings. Taken together, HRW treatment promoted growth of seedlings, by stimulating elongation of hypocotyl and root cells, via HRW-induced increase in GA and IAA content in the hypocotyl and the root respectively.

scholarly journals Laser Irradiation of Mung Bean (Vigna radiata L.) at Two Wavelengths for Enhanced Seedling Development

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Rayno Vic B. Janayon ◽  
Raphael A. Guerrero
Keyword(s):  
Laser Irradiation ◽  
Vigna Radiata ◽  
Mung Bean ◽  
Continuous Wave ◽  
Hypocotyl Length ◽  
Maximum Increase ◽  
Bean Seedling ◽  
Laser Sources ◽  
Level Of Significance ◽  
Seedling Characteristics

Two laser sources operating at wavelengths of 632.8 nm (He-Ne) and 488 nm (Ar+) are used in examining the effects of coherent, low-power, continuous wave exposure on the development of Vigna radiata L. Presowing laser irradiation of seeds leads to an improvement of mung bean seedling characteristics, with increased values of hypocotyl length (an increase of up to 22.5%), root length (an increase of up to 28.8%), and seedling mass (a maximum increase of 29.2%). Measured parameters between seedlings from laser-irradiated seeds and from control samples are statistically different at a level of significance equal to 0.05. For both laser wavelengths, an optimal exposure time of 2 minutes is determined for enhanced growth of mung bean seedlings.

Investigations of growth-promoting factors in conditioned soybean root cells and in the liquid medium in which they grow: ammonium, glutamine, and amino acids

1974 ◽  
Vol 52 (7) ◽  
pp. 1747-1755 ◽  
Author(s):  
P. A. Sargent ◽  
J. King
Keyword(s):  
Amino Acids ◽  
Dry Weight ◽  
Liquid Media ◽  
Root Cells ◽  
Nitrogenous Compounds ◽  
Soybean Root ◽  
Haplopappus Gracilis ◽  
Glycine Max L ◽  
Growth Promoting ◽  
Cells Cultured

Cells cultured in sterile, liquid media from a number of Phaseolus spp., soybean cotyledons, shoots, and roots and from rice explants grew, in terms of dry-weight increase, much better in the presence of NH4+ and NO3− as sources of nitrogen than with NO3− alone. Other cultures tested, including other legumes, either did not respond positively to added NH4+ or, as in the case of Haplopappus gracilis cells, grew better in its absence.Earlier it had been shown that soybean (Glycine max. L. cv. Mandarin) root cells grew better in the presence of NH4+ than in its absence and that 'conditioning' substances were produced by cells and excreted into the medium between about the 15th and 35th h of incubation. These observations and those above with other cell cultures led to the initiation of an investigation of why some cells respond to NH4+ while others do not.This investigation has so far taken the form of an analysis of nitrogenous compounds in soybean root cells and in the NH4+-containing medium in which they were grown during 120 h of incubation and especially after 24 h of incubation, the time of maximum production of growth-enhancing ability in both cells and medium.Growth enhancement can be accounted for, apparently, by the occurrence of residual NH4+ in conditioned medium and by the presumed occurrence of NH4+ in cells. However, glutamine and its derivatives are implicated in the conditioning process.

Synthesis and preliminary bioactivity evaluation of new pregnane brassinosteroid-like compounds

2005 ◽  
Vol 83 (8) ◽  
pp. 1084-1092 ◽  
Author(s):  
Daniel G Rivera ◽  
Francisco Coll
Keyword(s):  
Hydrogen Bond ◽  
Plant Growth ◽  
Hypocotyl Elongation ◽  
Hydrogen Bond Donor ◽  
Donor And Acceptor ◽  
Plant Growth Promoting ◽  
Plant Growth Promoting Activity ◽  
Growth Promoting ◽  
Cotyledon Expansion

Seven new pregnane compounds bearing some representative A- and B-ring brassinosteroid functions, as well as hydrogen bond donor and acceptor ones on the D ring, were efficiently synthesized. The obtained compounds did not show remarkable plant growth-promoting activity in the radish hypocotyl elongation and cotyledon expansion bioassays, however, introducing oxygen and amino functions on the D ring led to an enhancement of the bioactivity. The 16β-functionalized pregnane brassinosteroid-like compounds were slightly more active than the 16α-functionalized ones.Key words: steroids, brassinosteroids, pregnane analogues.

Evaluation of plant growth promoting rhizobacteria (PGPR) for the control of charcoal rot of Mung bean

2021 ◽  
Vol 54 (1) ◽  
Author(s):  
Amjid Khan ◽  
Shehzad Asad ◽  
Asghari Bano ◽  
Rashid Abbas Khan ◽  
Tauqeer Ahmed Qadri
Keyword(s):  
Plant Growth ◽  
Mung Bean ◽  
Plant Growth Promoting Rhizobacteria ◽  
Charcoal Rot ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Supraoptimal ethylene acts antagonistically with exogenous gibberellins during Solanum lycopersicum (Solanaceae) hypocotyl growth.

2016 ◽  
Vol 51 (2) ◽  
pp. 235-242 ◽  
Author(s):  
Francesco Mignolli ◽  
Graciela Beatriz Rojas ◽  
María Laura Vidoz
Keyword(s):  
Inhibitory Effect ◽  
Hypocotyl Elongation ◽  
Plant Growth And Development ◽  
Hypocotyl Growth ◽  
Hypocotyl Length ◽  
Constitutive Activation ◽  
Tomato Seedlings ◽  
Promotive Effect ◽  
Endogenous Ethylene ◽  
Exogenous Ethylene

In many plant species, ethylene and gibberellins interact to regulate plant growth and development. In some cases, these hormones can act in a synergistic way whereas in others they can be antagonistic. To date, the control of hypocotyl elongation by ethylene and gibberellins has been poorly explored in tomato. In this paper, we report that, application of exogenous ethylene to tomato seedlings or high endogenous ethylene production, as in the epinastic mutant, strongly prevent the effect of gibberellic acid (GA3) application. Moreover, constitutive activation of gibberellin signal in a DELLA deficient mutant is not able to counteract the inhibitory effect of ethylene on hypocotyl elongation, suggesting that ethylene acts independently from DELLA-mediated gibberellin response. Interestingly, when ethylene perception is blocked, the GA3 promotive effect on hypocotyl length is less effective, indicating that the presence of a basal level of ethylene could synergistically enhance hypocotyl growth. Taken together, these observations may suggest that, in tomato, supraoptimal concentrations of ethylene are able to antagonize gibberellin effect but normal levels seem to promote gibberellin-induced hypocotyl elongation.

scholarly journals Influence of GdVO4:Eu3+ Nanocrystals on Growth, Germination, Root Cell Viability and Oxidative Stress of Wheat (Triticum aestivum L.) Seedlings

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1187
Author(s):  
Anna Ekner-Grzyb ◽  
Jagna Chmielowska-Bąk ◽  
Agata Szczeszak
Keyword(s):  
Oxidative Stress ◽  
Triticum Aestivum ◽  
Germination Rate ◽  
Thiobarbituric Acid ◽  
Triticum Aestivum L ◽  
Root Cell ◽  
Lipid Peroxidation Product ◽  
Hypocotyl Length ◽  
Root Cells ◽  
Tetrazolium Chloride

The increasing application of lanthanide-doped nanocrystals (LDNCs) entails the risk of a harmful impact on the natural environment. Therefore, in the presented study the influence of gadolinium orthovanadates doped with Eu3+ (GdVO4:Eu3) nanocrystals on wheat (Triticum aestivum L.), chosen as a model plant species, was investigated. The seeds were grown in Petri dishes filled with colloids of LDNCs at the concentrations of 0, 10, 50 and 100 µg/mL. The plants’ growth endpoints (number of roots, roots length, roots mass, hypocotyl length and hypocotyl mass) and germination rate were not significantly changed after the exposure to GdVO4:Eu3+ nanocrystals at all used concentrations. The presence of LDNCs also had no effect on oxidative stress intensity, which was determined on the basis of the amount of lipid peroxidation product (thiobarbituric acid reactive substances; TBARS) in the roots. Similarly, TTC (tetrazolium chloride) assay did not show any differences in cells’ viability. However, root cells of the treated seedlings contained less Evans Blue (EB) when compared to the control. The obtained results, on the one hand, suggest that GdVO4:Eu3+ nanocrystals are safe for plants in the tested concentrations, while on the other hand they indicate that LDNCs may interfere with the functioning of the root cell membrane.

scholarly journals Genome Sequencing of a Mung Bean Plant Growth Promoting Strain ofP. aeruginosawith Biocontrol Ability

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Devaraj Illakkiam ◽  
Manoharan Shankar ◽  
Paramasivan Ponraj ◽  
Jeyaprakash Rajendhran ◽  
Paramasamy Gunasekaran
Keyword(s):  
Antifungal Activity ◽  
Plant Growth ◽  
Genome Sequencing ◽  
Mung Bean ◽  
Draft Genome ◽  
Hydrolytic Enzymes ◽  
Personal Genome ◽  
Cellulose Synthesis ◽  
Plant Growth Promoting ◽  
Growth Promoting

Pseudomonas aeruginosaPGPR2 is a mung bean rhizosphere strain that produces secondary metabolites and hydrolytic enzymes contributing to excellent antifungal activity againstMacrophomina phaseolina, one of the prevalent fungal pathogens of mung bean. Genome sequencing was performed using the Ion Torrent Personal Genome Machine generating 1,354,732 reads (6,772,433 sequenced bases) achieving ~25-fold coverage of the genome. Reference genome assembly using MIRA 3.4.0 yielded 198 contigs. The draft genome of PGPR2 encoded 6803 open reading frames, of which 5314 were genes with predicted functions, 1489 were genes of known functions, and 80 were RNA-coding genes. Strain specific and core genes ofP. aeruginosaPGPR2 that are relevant to rhizospheric habitat were identified by pangenome analysis. Genes involved in plant growth promoting function such as synthesis of ACC deaminase, indole-3-acetic acid, trehalose, mineral scavenging siderophores, hydrogen cyanide, chitinases, acyl homoserine lactones, acetoin, 2,3-butanediol, and phytases were identified. In addition, niche-specific genes such as phosphate solubilising 3-phytase, adhesins, pathway-specific transcriptional regulators, a diguanylate cyclase involved in cellulose synthesis, a receptor for ferrienterochelin, a DEAD/DEAH-box helicase involved in stress tolerance, chemotaxis/motility determinants, an HtpX protease, and enzymes involved in the production of a chromanone derivative with potent antifungal activity were identified.

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