Effect of Auxin, Fusicoccin and Tris Buffer on Ion Uptake, Organic Acid Synthesis and Cell Elongation in Barley Coleoptile Segments

1981 ◽  
Vol 8 (5) ◽  
pp. 375
Author(s):  
B Kholdebarin
Keyword(s):  
Organic Acids ◽  
Organic Acid ◽  
Cell Elongation ◽  
Acid Synthesis ◽  
Ion Uptake ◽  
Tris Buffer ◽  
Common Mechanism ◽  
K Uptake ◽  
Presence And Absence ◽  
Total Organic Acid

The effects of auxin, fusicoccin and tris buffer on K+ and Cl- uptake, total organic acids synthesis and cell elongation in barley coleoptile segments were tested. Some differences between the effects of fusicoccin and auxin on cell elongation and ion uptake were observed which support the idea that the two hormones may not be acting through a common mechanism. Tris buffer in the presence and absence of hormones stimulated K+ uptake and inhibited both Cl- absorption and to some extent cell elongation. Tris buffer treatments, which resulted in excess K+ uptake over Cl-, also caused a net synthesis of organic acids in the tissues. In some experiments, the relation between auxin-induced cell elongation and total organic acid synthesis was also examined.

scholarly journals Transcriptome Analysis Provides Insights into the Root Response of Chinese Fir to Phosphorus Deficiency

2021 ◽  
Author(s):  
Wanting Chen ◽  
Mengyan Zhou ◽  
Mingzhen Zhao ◽  
Ranhong Chen ◽  
Mulualem Tigabu ◽  
...  
Keyword(s):  
Citric Acid ◽  
Organic Acids ◽  
Organic Acid ◽  
Malic Acid ◽  
Glyoxylate Cycle ◽  
Acid Synthesis ◽  
Chinese Fir ◽  
Purple Acid Phosphatase ◽  
Timber Species ◽  
Pi Deficiency

Abstract Background: Phosphorus is one of the essential elements for plant growth and development, but the content of plant available phosphorus (Pi) in many soil types is low. As a fast-growing timber species, Chinese fir is in great demand of Pi, and the lack of Pi in soil restricts the increase of productivity of Chinese fir plantation. Root morphology and the synthesis and secretion of organic acids play an important role in the uptake of phosphorus, but the molecular mechanisms of Chinese fir in response to Pi deficiency are largely unexplored. Results: In this study, seedlings of Yang 061 clone were grown under three Pi supply levels (0, 5 and 10 mg·L-1 P) and morphological attributes, organic acid content and enzyme activity were measured, the transcriptome data of Chinese fir root system were obtained and the expression levels of phosphorus responsive genes and organic acid synthesis related genes on citric acid and glyoxylate cycle pathway were determined. The results showed that there were 50,808 Unigenes annotated from the transcriptome of Chinese fir roots. Among differentially expressed genes, seven genes of phosphate transporter family and seventeen genes of purple acid phosphatase family were up-regulated by Pi deficiency, two proteins of SPX domain were up-regulated and one was down-regulated. The metabolic pathways of the citric acid and glyoxylate cycle pathway were mapped, and the expression characteristics of the related Unigenes under different phosphorus treatments were analyzed. The genes involved in malic acid and citric acid synthesis were up-regulated, and the activities of the related enzymes were significantly enhanced under long-term stress. The contents of citric acid and malic acid in the roots of Chinese fir increased after 30days of Pi deficiency. Conclusion: The Chinese fir roots showed increased expression of citrate and malate synthesis genes, increased content of organic acids and enhanced activities of related enzymes under Pi deficiency. The results provide a new insight for revealing the molecular mechanism of Pi deficiency and the pathway of organic acid synthesis in Chinese fir roots.

scholarly journals Transcriptome analysis provides insights into the root response of Chinese fir to phosphorus deficiency

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Wanting Chen ◽  
Mengyan Zhou ◽  
Mingzhen Zhao ◽  
Ranhong Chen ◽  
Mulualem Tigabu ◽  
...  
Keyword(s):  
Citric Acid ◽  
Organic Acids ◽  
Organic Acid ◽  
Malic Acid ◽  
Glyoxylate Cycle ◽  
Phosphorus Deficiency ◽  
Acid Synthesis ◽  
Chinese Fir ◽  
Purple Acid Phosphatase ◽  
Pi Deficiency

Abstract Background Phosphorus is one of the essential elements for plant growth and development, but available phosphorus (Pi) content in many soil types is low. As a fast-growing tree species for timber production, Chinese fir is in great demand of Pi, and the lack of Pi in soil restricts the increase of productivity of Chinese fir plantation. Root morphology and the synthesis and secretion of organic acids play an important role in the uptake of phosphorus, but the molecular mechanisms of Chinese fir root responses to Pi deficiency are largely unexplored. In this study, seedlings of Yang 061 clone were grown under three Pi supply levels (0, 5 and 10 mg·L-1 P) and morphological attributes, organic acid content and enzyme activity were measured. The transcriptome data of Chinese fir root system were obtained and the expression levels of phosphorus responsive genes and organic acid synthesis related genes on citric acid and glyoxylate cycle pathway were determined. Results We annotated 50,808 Unigenes from the transcriptome of Chinese fir roots. Among differentially expressed genes, seven genes of phosphate transporter family and 17 genes of purple acid phosphatase family were up-regulated by Pi deficiency, two proteins of SPX domain were up-regulated and one was down-regulated. The metabolic pathways of the citric acid and glyoxylate cycle pathway were mapped, and the expression characteristics of the related Unigenes under different phosphorus treatments were analyzed. The genes involved in malic acid and citric acid synthesis were up-regulated, and the activities of the related enzymes were significantly enhanced under long-term Pi stress. The contents of citric acid and malic acid in the roots of Chinese fir increased after 30 days of Pi deficiency. Conclusion Chinese fir roots showed increased expression of genes related with phosphorus starvation, citrate and malate synthesis genes, increased content of organic acids, and enhanced activities of related enzymes under Pi deficiency. The results provide a new insight for revealing the molecular mechanism of adaption to Pi deficiency and the pathway of organic acid synthesis in Chinese fir roots.

scholarly journals PSVIII-16 The effects of adding two organic acid blends with or without high levels of zinc or copper, on post-weaning pig performance

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 209-210
Author(s):  
Casey L Bradley ◽  
Jon Bergstrom ◽  
Jeremiah Nemechek ◽  
J D Hahn
Keyword(s):  
Phosphoric Acid ◽  
Benzoic Acid ◽  
Organic Acids ◽  
Organic Acid ◽  
Factorial Design ◽  
Sodium Butyrate ◽  
Weaned Pigs ◽  
Weaning Pig ◽  
Pig Performance ◽  
Dietary Treatments

Abstract A subset of 720 weaned pigs (6.44 ± 0.1 kg, PIC genetics, approximately 21-d of age) were used in a 42-d trial with a 2x3 factorial design evaluating the effects of adding organic acid (OA) blends [factor 1 = no organic acid (NO), Acid Pak 1 (AP1), Acid Pak 2 (AP2)] to diets with or without higher levels of Zn or Cu [factor 2 = +/-PZC] on pig performance. Pigs were allotted 10 pigs/pen to 12 weight blocks and randomly assigned the six dietary treatments. The +PZC diets contained 3000 ppm Zn (d 0-7), 2000 ppm Zn (d 8-21), and 250 ppm Cu (d 21-42) and -PZC diets contained 95 ppm Zn and 20 ppm Cu (d 0-42). The AP1 and AP2 diets used 0.9% of 2 acid premixes (d 0-21), and 0.45% of the premixes (day 22-42). AP1 provided 0.5% benzoic acid, 0.07% sodium butyrate, and 0.025% phosphoric acid (day 0-21) and half those levels (day 22-42). AP2 included the same acids as AP1 but at half the rate and combined with 7 other organic acids and carvacrol. From d 0-21, ADG, ADFI, and G:F were improved (P< 0.01) by +PZC compared to -PZC and by AP1 or AP2 compared to NO (P< 0.02). Overall (d 0-42), ADG and G:F were improved (P< 0.01) by +PZC compared to -PZC and by AP1 or AP2 compared to NO (P< .010). Data from this trial indicate that performance was improved by the addition of both OA and PZC. However, pigs fed OA and -PZC performed similarly to those fed NO and +PZC in the post-weaning period. In summary, regardless of the acid combination, organic acid supplementation has the potential to improve growth performance in weaned pigs.

scholarly journals Carboxylate anion generation in aqueous solution from carbonate radiolysis, a potential route for abiotic organic acid synthesis on Earth and beyond

2021 ◽  
Vol 564 ◽  
pp. 116892
Author(s):  
Johan Vandenborre ◽  
Laurent Truche ◽  
Amaury Costagliola ◽  
Emeline Craff ◽  
Guillaume Blain ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Organic Acid ◽  
Acid Synthesis ◽  
Carboxylate Anion

The Xylem Sap of Maize Roots: Its Collection, Composition and Formation

1988 ◽  
Vol 15 (4) ◽  
pp. 557 ◽  
Author(s):  
MJ Canny ◽  
ME Mccully
Keyword(s):  
Amino Acids ◽  
Organic Acids ◽  
Organic Acid ◽  
Aspartic Acid ◽  
Xylem Sap ◽  
Great Variability ◽  
Reduced Pressure ◽  
Transpiration Stream ◽  
Maize Roots ◽  
Total Amino Acids

Three methods of sampling xylem sap of maize roots were compared: sap bleeding from the stem cut just above the ground; sap bleeding from the cut tops of roots still undisturbed in the ground; and sap aspirated from excavated roots under reduced pressure. The bleeding saps were often unobtainable. When their composition was measured with time from cutting, the concentrations of the major solutes approximately doubled in 2 h. Aspirated sap was chosen as the most reliable sample of root xylem contents. Solute concentrations of the saps showed great variability between individual roots for all solutes, but on average the concentrations found (in �mol g-1 sap) were: total amino acids, 1.8; nitrate, 1.8; sugars (mainly sucrose), 5.4; total organic acids, 18.3. Individual amino acids also varied greatly between roots. Glutamine, aspartic acid and serine were generally most abundant. The principal organic acid found was malic, approximately 8 �mol g-1. From these analyses the ratios of carbon in the fractions (sugars : amino acids : organic acids) = (44 : 6 : 50). 14Carbon pulse fed to a leaf appeared in the root sap within 30 min, rose to a peak at 4-6 h, and declined slowly over a week. During all this time the neutral, cation and anion fractions were sensibly constant in the proportions 86 : 10 : 4. The 14C therefore did not move towards the equilibrium of 12C-compounds in the sap. It is argued that the results do not support a hypothesis of formation of amino carbon from recent assimilate and reduced nitrate in the roots and an export of this to the shoot in the transpiration stream.

scholarly journals Characterization of Aerosol Composition, Aerosol Acidity and Organic Acid Partitioning at an Agriculture-Intensive Rural Southeastern U.S. Site

2018 ◽  
Author(s):  
Theodora Nah ◽  
Hongyu Guo ◽  
Amy P. Sullivan ◽  
Yunle Chen ◽  
David J. Tanner ◽  
...  
Keyword(s):  
Oxalic Acid ◽  
Organic Acids ◽  
Organic Acid ◽  
Organic Aerosol ◽  
Water Soluble ◽  
Molar Concentration ◽  
Particle Phase ◽  
Aerosol Composition ◽  
Phase Water ◽  
Acetic Acids

Abstract. The implementation of stringent emission regulations has resulted in the decline of anthropogenic pollutants including sulfur dioxide (SO2), nitrogen oxides (NOx) and carbon monoxide (CO). In contrast, ammonia (NH3) emissions are largely unregulated, with emissions projected to increase in the future. We present real-time aerosol and gas measurements from a field study conducted in an agricultural-intensive region in the southeastern U.S. during the fall of 2016 to investigate how NH3 affects particle acidity and SOA formation via the gas-particle partitioning of semi-volatile organic acids. Particle water and pH were determined using the ISORROPIA-II thermodynamic model and validated by comparing predicted inorganic HNO3-NO3− and NH3-NH4+ gas-particle partitioning ratios with measured values. Our results showed that despite the high NH3 concentrations (study average 8.1 ± 5.2 ppb), PM1 were highly acidic with pH values ranging from 0.9 to 3.8, and a study-averaged pH of 2.2 ± 0.6. PM1 pH varied by approximately 1.4 units diurnally. Formic and acetic acids were the most abundant gas-phase organic acids, and oxalate was the most abundant particle-phase water-soluble organic acid anion. Measured particle-phase water-soluble organic acids were on average 6 % of the total non-refractory PM1 organic aerosol mass. The measured molar fraction of oxalic acid in the particle phase (i.e., particle-phase oxalic acid molar concentration divided by the total oxalic acid molar concentration) ranged between 47 and 90 % for PM1 pH 1.2 to 3.4. The measured oxalic acid gas-particle partitioning ratios were in good agreement with their corresponding thermodynamic predictions, calculated based on oxalic acid’s physicochemical properties, ambient temperature, particle water and pH. In contrast, gas-particle partitioning of formic and acetic acids were not well predicted for reasons currently unknown. For this study, higher NH3 concentrations relative to what has been measured in the region in previous studies had minor effects on PM1 organic acids and their influence on the overall organic aerosol and PM1 mass concentrations.

scholarly journals Understanding the anthropogenic influence on formation of biogenic secondary organic aerosols in Denmark via analysis of organosulfates and related oxidation products

2014 ◽  
Vol 14 (17) ◽  
pp. 8961-8981 ◽  
Author(s):  
Q. T. Nguyen ◽  
M. K. Christensen ◽  
F. Cozzi ◽  
A. Zare ◽  
A. M. K. Hansen ◽  
...  
Keyword(s):  
Organic Acids ◽  
Long Range ◽  
Secondary Organic Aerosols ◽  
Global Radiation ◽  
Organic Aerosols ◽  
Photochemical Reactions ◽  
Common Mechanism ◽  
Aerosol Acidity ◽  
Local Impacts ◽  
Concentration Levels

Abstract. Anthropogenic emissions of sulfur dioxide (SO2) and nitrogen oxides (NOx) may affect concentration levels and composition of biogenic secondary organic aerosols (BSOA) through photochemical reactions with biogenic organic precursors to form organosulfates and nitrooxy organosulfates. We investigated this influence in a field study from 19 May to 22 June, 2011 at two sampling sites in Denmark. Within the study, we identified a substantial number of organic acids, organosulfates and nitrooxy organosulfates in the ambient urban curbside and semi-rural background air. A high degree of correlation in concentrations was found among a group of specific organic acids, organosulfates and nitrooxy organosulfates, which may originate from various precursors, suggesting a common mechanism or factor affecting their concentration levels at the sites. It was proposed that the formation of those species most likely occurred on a larger spatial scale, with the compounds being long-range transported to the sites on the days with the highest concentrations. The origin of the long-range transported aerosols was investigated using the Hybrid Single Particle Lagrangian Integrated Trajectory (HYSPLIT) model in addition to modeled emissions of related precursors, including isoprene and monoterpenes using the global Model of Emissions of Gases and Aerosols from Nature (MEGAN) and SO2 emissions using the European Monitoring and Evaluation Program (EMEP) database. The local impacts were also studied by examining the correlation between selected species, which showed significantly enhanced concentrations at the urban curbside site and the local concentrations of various gases, including SO2, ozone (O3), NOx, aerosol acidity and other meteorological conditions. This investigation showed that an inter-play of the local parameters such as the aerosol acidity, NOx, SO2, relative humidity (RH), temperature and global radiation seemed to affect the concentration level of those species, suggesting the influence of aqueous aerosol chemistry. The local impacts, however, seemed minor compared to the regional impacts. The total concentrations of organosulfates and nitrooxy organosulfates, on average, contributed to approximately 0.5–0.8% of PM1 mass at the two sampling sites.

STUDIES ON INFANT DIARRHEA. III. CHANGES IN COMPOSITION OF SALINE AND GLUCOSE-SALINE SOLUTIONS INSTILLED INTO THE COLON

PEDIATRICS ◽  
1968 ◽  
Vol 42 (2) ◽  
pp. 303-311
Author(s):  
R. Torres-Pinedo ◽  
E. Conde ◽  
G. Robillard ◽  
M. Maldonado
Keyword(s):  
Organic Acids ◽  
Organic Acid ◽  
The Body ◽  
Hydrogen Ion ◽  
Sodium Absorption ◽  
Infectious Diarrhea ◽  
Subsequent Formation ◽  
Clear Cut ◽  
Compositional Changes ◽  
Saline Solutions

Saline and glucose-saline solutions were instilled into the distal colons of infants with acute infectious diarrhea. Samples of the fluid were obtained at hourly intervals. Clear-cut differences in compositional changes were observed with the saline and glucose-saline solutions. The net effects induced by glucose were: (1) generation of organic acids and subsequent formation of poorly absorbable organic acid salts, and (2) osmotic inflow of water. The overall process led to a net gain of hydrogen ion by the body fluids, decrease in sodium absorption, augmented potassium loss, and net increase in volume of the colonic fluid.

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