7 ARTIFICIAL INSEMINATION IN BOS INDICUS WITH SEMEN IN SIMULTANEOUS POOL OF MULTIPLE BULLS

2010 ◽  
Vol 22 (1) ◽  
pp. 161
Author(s):  
J. R. S. Torres-Júnior ◽  
J. A. T. Souza ◽  
M. I. S. Silva ◽  
J. S. Costa ◽  
G. S. Lima ◽  
...  
Keyword(s):  
Body Condition Score ◽  
Bos Indicus ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Sao Paulo ◽  
Conception Rate ◽  
São Paulo ◽  
Conventional Dose ◽  
Conception Rates ◽  
Time Required

The duration of spermatic viability in the female reproductive system in cattle is 18 to 24 hours. Therefore, the time required for sperm capacitation in different bulls demonstrated to be highly variable and may affect the adequate amount of capacitated sperm within an optimal window of time for fertilization. The aim of this study was to evaluate the conception rate in Nelore cows inseminated with semen from a single bull compared with semen from a pool of 3 bulls (Fertility Plus®, ABS Pecplan, Minas Gerais, Brazil). On a random day of the estrous cycle (Day 0), 400 Nelore multiparous cows received a progesterone intravaginal device (1 g of P4, DIB®, Intervet/Schering-Plough, Cotia, Brazil) plus 2mg of estradiol benzoate (i.m. Estrogin®, Farmavet, São Paulo, Brazil). At the time of P4 device withdrawal (Day 8), 150 (ig of d-cloprostenol (i.m. Preloban®, Intervet/Schering-Plough), 1 mg of estradiol cypionate (i.m. ECP®, Pfizer, Brazil), and 300IU of eCG (i.m. Folligon®, Intervet/Schering-Plough) were administered. At 48 to 52 h after implant removal, all cows were fixed-time inseminated (FTAI). Then, cows were homogeneously distributed based on the semen used for FTAI in 4 groups: CONV1 (conventional dose of semen from bull 1), CONV2 (conventional dose of semen from bull 2), CONV3 (conventional dose of semen from bull 3), FERT (pool of semen from bulls 1, 2 and 3 homogeneously mixed in a single dose). Both conventional and Fertility Plus batches of semen contained a total dose of 30 × 106 spermatozoa. For pregnancy diagnosis, an ultrasonographic (CHISON 500VET, USP Brasil Eletromedicina, São Paulo, Brazil) examination was performed 30 days after the FTAI. Data were analyzed using the GENMOD procedure of SAS (SAS Institute, Cary, NC, USA). There was no interaction between treatment and body condition score [using a 1 (very thin) to 5 (very fat) point scale] or AI technician. The conception rates for CONV1, CONV2, CONV3, and FERT were, respectively, 40/101 (39.6%)b, 50/99 (50.5%)ab, 46/99 (46.5%)ab, 54/101 (53.5%)a; P < 0.05. In addition, the mean of conventional v. Fertility Plus treatments presented conception rates of 136/299 (45.5%) v. 54/101 (53.5%), respectively (P = 0.14). No significant statistical differences were observed between treatments. However, this trial showed that the utilization of the new Fertility Plus technology increased the overall conception rate by 8.0%. This probably implicates a complementary effect among semen samples and provides a greater time of sperm viability in oviduct. Also it provides evidence of the individual variation in capacitation time of bulls used for composition of the pool. Pooled semen of multiple sires may improve the reproductive performance at AI in cattle and increase the potential of the beef industry to produce pregnancies and born calves.

8 EFFECT OF SEMEN TYPES (SEXED v. NONSEXED) ON CONCEPTION RATES OF GIROLANDO HEIFERS INSEMINATED IN FIXED-TIME AI

2010 ◽  
Vol 22 (1) ◽  
pp. 162
Author(s):  
J. Almeida ◽  
O. A. Resende ◽  
D. L. Silveira
Keyword(s):  
Body Condition Score ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Sao Paulo ◽  
São Paulo ◽  
Device Removal ◽  
Conception Rates ◽  
Sexed Semen ◽  
Condition Score ◽  
Intravaginal Device

With the goal of optimizing protocols, fixed-time artificial insemination (FTAI) was performed under field conditions in a study to evaluate conception rates in Girolando (5/8) heifers (18 to 24 months of age) treated with estradiol benzoate (BE) to induce ovulation. Forty heifers were allocated to 2 groups with 20 animals each: GI-sexed semen (2 million spermatozoa/dose) and GII-nonsexed semen (20 million spermatozoa/dose), with body condition score (BCS) of 3.0 ± 0.14 (scale of 1 to 5), middleweight of 335 ± 38 kg, so that it was made for reutilization of progesterone device. On Day 0 (morning), the animals received 2.0 mg of BE (RIC-BE®,Tecnopec, São Paulo, Brazil), 0.075 mg of D-cloprostenol (Prolise®, ARSA S.R.L., Buenos Aires, Argentina), and an intravaginal device of 1 g of progesterone (Primer®, Tecnopec). On Day 8 (morning) the device was removed and 0.075 mg of D-cloprostenol was managed in the GI animals, then these animals received 1.0 mg of BE 24 h after device removal. The insemination was in the late afternoon of Day 10 with a 6-hour delay (4 to 6 pm). The GII animals received Primer previously used for 8 days (being used in the GI animals under the same protocol). After pregnancy diagnosis at 60 days, a third group was created (GIII) using the not-pregnant animals of GI and GII. In GIII, Primer from third use [disinfected in solution Kilol®-L 1:250 (Quinabra, São Paulo, Brazil) and rinsed with water, dried, and packed in plastic sacks and kept for 60 days] was used following the GI protocol with sexed semen. There were no significant differences (P > 0.05) in the conception rates of 40, 58, and 65% among groups GI (8/20), GII (11/20), and GIII (13/20). These results indicate very good conception rates for heifers, contrary to recommendations of commercial companies of sexed semen that alert for the conception rate for mated animals to be lower when using FTAI and mating protocols in mass, compared with that of AI after estrus observation.

178 USE OF A NEW INTRAVAGINAL DEVICE TO RELEASE PROGESTERONE IN FIXED-TIME EMBRYO TRANSFER EMBRYO RECIPIENTS

2010 ◽  
Vol 22 (1) ◽  
pp. 247
Author(s):  
J. R. V. Pimentel ◽  
P. H. P. Miguez ◽  
A. Kehrle ◽  
E. H. Madureira ◽  
F. A. Braga ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Body Condition Score ◽  
Block Design ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Sao Paulo ◽  
P Value ◽  
São Paulo ◽  
Condition Score ◽  
Intravaginal Device

Progestagen-releasing devices are commonly used for fixed-time artificial insemination and fixed-time embryo transfer (FTET). The aim of this study was to evaluate the efficacy of a new progesterone-releasing intravaginal device made of poly(3-hydroxy)butyrate-valerate copolymer (PHB-V) and poly-ϵ-caprolactone (PCL; Progestar®, Innovare, São Paulo, Brazil) to synchronize crossbredcows and heifers for FTET by determining ovulation, conception, and pregnancy rates. A total of 205 recipients ranging in age from 2 to 8 years (heifers, n = 77; non-suckled cows, n = 69; suckled cows, n = 59) were used on 3 different farms during February, March, and April 2008. The experimental design was a generalized block design (cyclicity, body condition score, postpartum interval, and animal category, i.e. heifers, suckled, or non-suckled cows), with 2 different synchronization treatments for FTET (Progestar® v. Crestar®). Cows were assigned to either Crestar® (norgestomet ear implants; Intervet Schering-Plough, Kenilworth, NJ, USA; n = 103) or Progestar® (PHB-V; n = 102) treatments. All groups were treated with 2 mg of estradiol benzoate i.m. (Innovare) and received the intravaginal or auricular device according to their group (Day 0). Together with P4 device withdrawal (Day 8), all animals received 400 IU of eCG i.m. (Novormon®, Schering-Plough) and 500 μg i.m. of PGF2α, (Sincrocio®, Ouro Fino, Brasil). On Day 9, all cows were treated with 1 mg of estradiol benzoate (Innovare) and the innovulation was performed on Day 17. Embryos were maintained at 37°C during the innovulation period. The percentage of usable recipients (AR) based on the presence of a functional CL on the day of embryo transfer, conception rate (numbers of pregnant recipients of the total number transferred; CR), and pregnancy rate (numbers of pregnant recipients of the total number synchronized; PR) were determined by ultrasonography 23 days after embryo transfer. Data were submitted to ANOVA (PROC GLM, SAS Institute Inc., Cary, NC, USA) and significant differences were considered to have a P-value of <0.05. None of the interactions were significant and there was no effect of treatment on AR (72.8% v. 83.3%), CR (47.9% v. 42.7%), and PR (34.7% v. 35.0%) for Progestar® v. Crestar® groups, respectively. This experiment showed that Progestar® was as efficacious as Crestar® to synchronize crossbred cows and heifers for FTET. FAPESP-Fundação de Amparo à Pesquisa do Estado de São Paulo and CNPq-Conselho Nacional de Desenvolvimento Científico e Tecnológico.

32 USE OF HORMONE WITH ACTION FOLLICLE-STIMULATING IN OVULATION SYNCHRONIZATION OF CROSSBRED HOLSTEIN-GYR COWS

2010 ◽  
Vol 22 (1) ◽  
pp. 174
Author(s):  
V. A. Nascimento ◽  
G. S. Moura ◽  
M. Dias ◽  
F. A. Oliveira ◽  
A. R. Dias ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Body Condition ◽  
Buenos Aires ◽  
Body Condition Score ◽  
Animal Health ◽  
Live Weight ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Sao Paulo ◽  
São Paulo

The objective of this study was to evaluate the administration of the follicle-stimulating hormone (FSH-p) or equine chorionic gonadotropin (eCG) in protocols of ovulation synchronization. One hundred forty-nine crossbred Holstein-Gyr cows were used in 2 properties in Minas Gerais and 3 treatments: TControl (n = 49 cows), Day 0: insert of a progesterone device (Primer®, Tecnopec, São Paulo, Brazil) + 2 mg of estradiol benzoate (RIC-BE®); Day 8: removal of Primer + 112 mg of D-cloprostenol (Prolise®, ARSA SRL, Buenos Aires, Argentina) + 1 mg of estradiol benzoate and fixed-time artificial insemination (FTAI) accomplished 44 h after the removal of Primer; TFSH (n = 50 cows), similar to TControl, but on Day 8, 15 mg of FSH-p (Folltropin®, Bioniche Animal Health) was administered; TeCG (n = 50 cows), similar to TControl, with administration on Day 8 of 300 IU of eCG (Novormon®, Syntex SA, Buenos Aires, Argentina). Cows that returned to estrus 14 to 28 days after the FTAI were inseminated once again. For all statistical analysis, we used the program SAS 9.0 (2002; SAS Institute Inc., Cary, NC, USA) at 5% probability. Quantitative variables (live weight, days postpartum, follicular average per cow) were analyzed by variance, and body condition score by Friedman test. The pregnancy rates were submitted to logistic analysis considering the effect of local experimental factors and were analyzed by Spearman correlation among the variables studied and the pregnancy rate. There was no difference (P > 0.05) between treatment for pregnancy rate in FTAI protocols (TControl = 40.82%; TFSH = 38.00%; TeCG = 26.00%), in the first insemination after FTAI (TControl = 56.25%; TFSH = 70.59%; TeCG = 68.42%), and in FTAI plus first insemination after FTAI (TControl = 59.18%; TFSH =62.00%; TeCG = 52.00%), as well as for the estrus repetition (TControl = 55.17%; TFSH = 54.84%; TeCG = 51.35%) and the correlation (P > 0.05) among the pregnancy rate and postpartum period, milk production, body weight and body condition. No effect was observed on the pregnancy rate by the addition of the hormones FSH and eCG in the protocol of ovulation synchronization in crossbred Holstein-Gyr cows; however, reinsemination in the period from 14 to 28 days after FTAI increased the pregnancy rate. CNPq; FAPEMIG; TECNOPEC/São Paulo, Brazil.

scholarly journals 215SUCCESSFUL OUT-OF-BREEDING SEASON ESTRUS SYNCHRONIZATION FOLLOWED BY FIXED TIME INSEMINATION IN WATERBUFFALO (BUBALUS BUBALIS)

2004 ◽  
Vol 16 (2) ◽  
pp. 229 ◽  
Author(s):  
R.M. Züge ◽  
U. Rodacki ◽  
A.T. Grandi ◽  
J.M.J. Aerts ◽  
P.E.J. Bols
Keyword(s):  
Breeding Season ◽  
Buenos Aires ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Sao Paulo ◽  
Reproductive Efficiency ◽  
São Paulo ◽  
Estrus Synchronization ◽  
The Impact ◽  
Synchronization Protocol

The most important barrier to the increase of buffalo productivity is an overall poor reproductive efficiency, characterized by late sexual maturity, seasonal anestrus and long periods of postpartum ovarian inactivity resulting in extended calving intervals and poor expression of estrus behavior (Singh J et al., 2000, Anim. Reprod. Sci. 60–61, 593–604). Buffaloes are seasonal breeders with the highest reproductive activities during winter (short day lengths) and a high frequency of anestrus during the summer months (Singh G et al. 1985, Ind. J. Anim. Res. 19, 57–60). Recent research demonstrated that a combination of progesterone, estradiol benzoate and equine chorionic gonadotropin (eCG) was effective for estrus induction and synchronization in buffalo heifers under Mediterranean conditions (Barile et al. 2001, Livestock Prod. Sci. 68, 283–287). The aim of the present study was to investigate the impact of an estrus synchronization protocol on reproductive efficiency of water buffalo during out of the normal breeding season. A total of six heifers (21 to 23 months of age) and three cows (5, 6 and 18 years of age) were enrolled in an estrus synchronization protocol lasting for 12 days. All animals were kept under tropical conditions in the coastal part of Paraná (Antonina), about 450km south of São Paulo. The experiment was performed in December, 2002, during the Brazilian summer season, when reproductive efficiency of buffaloes is greatly reduced. On the first day of the protocol (Day 0), animals were implanted with an intravaginal device containing 1g of progesterone (DIB, Syntex SA, Buenos Aires, Argentina) and injected with 10mg estradiol benzoate (Estrogin, Famavet, São Paulo, Brazil). On Day 9, the DIB implant was removed and the animals received 150μg (i.m.) of cloprostenol (Prolise, Syntex SA, Buenos Aires, Argentina) and 2500 IU of eCG (Novormon, Syntex SA, Buenos Aires, Argentina). On Day 11, all animals received 1500 IU of hCG (Vetecor, Lab. Calier, Spain). Artificial insemination (AI) was performed on Day 12 using frozen-thawed semen from a bull of proven fertility. Only one AI was performed per heifer/cow. Pregnancies were determined by ultrasound examination at 53 days following AI and confirmed by rectal palpation at 90 days post AI. The use of this estrus synchronization protocol, followed by fixed-time insemination, resulted in four pregnant heifers (66%) and three pregnant cows (100%). Our results demonstrate that buffalo reproduction can be successful during out-of-breeding season when adequate hormonal treatment is used. Additional experiments should be done to validate the protocol.

210 FACTORS AFFECTING CONCEPTION RATE IN LACTATING HOLSTEIN RECIPIENTS

2007 ◽  
Vol 19 (1) ◽  
pp. 222
Author(s):  
D. T. G. Jardina ◽  
C. A. Rodrigues ◽  
J. L. M. Vasconcelos
Keyword(s):  
Embryonic Development ◽  
Embryo Quality ◽  
Estradiol Benzoate ◽  
Sao Paulo ◽  
Conception Rate ◽  
Embryonic Mortality ◽  
São Paulo ◽  
Factors Affecting ◽  
Size Classification ◽  
Rectal Palpation

This study evaluated embryo transfers from Holstein donors in lactating Holstein recipients. It was performed at the Santa Rita Farm, Descalvado, SP, Brazil, between January 2005 and January 2006. Holstein recipients were milked 3 times a day and fed with total mixed ration. Embryos were obtained by a superstimulation protocol with two 3-mg norgestomet ear implants (Crestar�; Intervet, Sao Paulo, Brazil) and an IM injection of 3 mg of estradiol benzoate (EB) on random days of the estrous cycle. Four days later, 500 IU FSH (Pluset�; Laboratorios Calier de Argentina) was divided into 8 decreasing doses given IM 12 h apart. With the seventh FSH injection, 0.530 mg of sodium cloprostenol (PGF2�, Ciosin�; Coopers Brazil, Sao Paulo, Brazil) was given IM; implants were removed with the eighth FSH injection. Twelve hours later, 250 �g gonadorelin (Fertagyl�: Intervet, Brazil) was given IM, followed by two AI 12 and 24 h later. Ova/embryos were recovered 6.5 days after the first insemination. Recipients (n = 2543) received an injection of PGF2� and were observed in estrus (n = 1322). Presence of CL was evaluated by rectal palpation of cows that presented heat, and embryo transfers (n = 873) were performed with a nonsurgical method 6 to 8 days after heat. Pregnancy diagnoses were performed by ultrasonography (Aloka 500, probe 5.0 MHz; Aloka) on Days 25 and 39. Embryonic mortality was considered when any recipient pregnant on Day 25 was open on Day 39. Data were analyzed by logistic regression and variables included in the model were: donor status (lactating or non-lactating), recipient CL external size classification (1 &gt; 2 &gt; 3), donor–recipient synchrony (-1, 0, +1), fresh or frozen embryos, embryonic development (Mo, Bi, Bl, Bx – IETS), and embryo quality (degree 1, 2, 3 – IETS). The conception rate (CR) on Day 25 was influenced by donor–recipient synchrony (-1: 37.7%; 0: 46.9%; +1: 45.6%; P = 0.053). Embryonic development (Mo: 45.6%; Bi: 41.8%; Bl: 46.9%; Bx: 43.8%), embryo quality (1: 44.2%; 2: 47.4%; 3: 42.3%), CL external size classification (CL 1: 47.9%; CL 2: 43.5%; CL 3: 30.0%), embryo condition (fresh: 45.8%; frozen: 43.1%), and donor status (lactating: 45.5%; non-lactating: 47.08%) did not affect conception rate. The CR on Day 39 was also affected by donor–recipient synchrony (-1: 29.9%; 0: 34.9%; +1: 38.2%; P = 0.021). Embryonic mortality was affected by donor status (lactating, 16.6% vs. non-lactating, 24.0%; P &lt; 0.10). These data suggest that donor–recipient synchrony is a major factor affecting conception rate in lactating Holstein recipients.

39 EFFECT OF TIME OF FIXED-TIME AI ON PREGNANCY RATES IN ZEBU CROSS-BRED BEEF HEIFERS TREATED WITH PROGESTERONE RELEASING DEVICES AND ESTRADIOL CYPIONATE

2010 ◽  
Vol 22 (1) ◽  
pp. 177 ◽  
Author(s):  
M. Ramos ◽  
L. Cutaia ◽  
P. Chesta ◽  
G. A. Bó
Keyword(s):  
Bos Taurus ◽  
Body Condition Score ◽  
Bos Indicus ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Beef Heifers ◽  
Treatment Groups ◽  
Condition Score ◽  
To Receive

Two experiments were designed to evaluate the effect of the timing of fixed-time AI (FTAI) in relation to the removal of an intravaginal progesterone-releasing device (1 g of progesterone, DIB, Syntex SA, Buenos Aires, Argentina) on pregnancy rates in Bos indicus × Bos taurus cross-bred heifers. In experiment 1, 285 Bonsmara × zebu cross-bred heifers, between 18 and 24 months of age and with a body condition score (BCS) between 3.0 and 3.5 (1-5 scale) were used. On the day of initiation of treatment (Day 0), the heifers’ ovaries were palpated (92% of them had a CL) and they received a new DIB plus 2 mg of estradiol benzoate (EB; Syntex SA) and 250 μg of cloprostenol (Ciclase DL, Syntex SA). On Day 8, DIB devices were removed and all heifers received 250 μg of Ciclase plus 0.5 mg of estradiol cypionate (ECP; Cipiosyn, Syntex SA). At that time the heifers were randomly divided to receive FTAI between 48 to 49 h, 53 to 54 h, or 58 to 59 h after DIB removal. The heifers underwent FTAI with semen from 4 bulls by 2 inseminators. In experiment 2, 260 heifers from the same group as those used in experiment 1 (87% with a CL) were treated exactly as those in experiment 1, except that previously used DIB was inserted on Day 0. Pregnancy diagnosis was performed 30 days post-fixed-time AI by ultrasonography. The data were analyzed by logistic regression, taking into account the effect of time of FTAI, semen, and inseminator on pregnancy rates. In experiment 1, pregnancy rates were lower (P = 0.04) in the heifers undergoing FTAI between 48 and 49 h after DIB removal (46/95, 48.4%) than those undergoing FTAI 53 to 54 h (61/99, 61.6%) or 58 to 60 h (57/91, 62.6%) after DIB removal. However, no differences in pregnancy rates were found (P = 0.72) in experiment 2 between the 3 treatment groups, with 39/91 (42.9%) for the 48 to 49 h group, 45/89 (50.6%) for the 53 to 54 h group, and 35/89 (43.8%) for the 58 to 59 h group. There was no effect of the semen or inseminator (P > 0.2) in either experiment. We conclude that when Bos indicus × Bos taurus beef heifers are synchronized with new DIB devices and ECP, higher pregnancy rates are obtained in heifers undergoing FTAI late (between 53 to 60 h after DIB removal) than in those undergoing FTAI early (48 to 49 h after DIB removal). However, time of insemination does not apparently affect pregnancy rates when Bos indicus × Bos taurus beef heifers are synchronized with previously used DIB devices and ECP.

13 USE OF A NEW INTRAVAGINAL DEVICE TO RELEASE PROGESTERONE IN FIXED-TIMED ARTIFICIAL INSEMINATION IN NELLORE COWS (BOS TAURUS INDICUS)

2010 ◽  
Vol 22 (1) ◽  
pp. 164
Author(s):  
F. A. Braga ◽  
F. J. D. Pardo ◽  
P. H. Miguez ◽  
A. Kehrle ◽  
J. R. V. Pimentel ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Body Condition Score ◽  
Estradiol Benzoate ◽  
Sao Paulo ◽  
Pregnancy Rates ◽  
Control Group ◽  
São Paulo ◽  
Effect Of Treatment ◽  
Intravaginal Device

Artificial insemination results can be improved using fixed-timed AI (FTAI). Currently, progesterone release devices, indispensable in FTAI procedures, such as DIB® (Schering-Plough, Kenilworth, NJ, USA), are made of silicone, a nonbiodegradable biocompatible polymer. Recently, biodegradable progesterone (P4)-releasing devices made of poly(3-hydroxy)butyrate-valerate copolymer (PHB-V) and poly-ϵ-caprolactona (PCL) have been developed. To investigate the efficiency on pregnancy rate of FTAI programs using a new intravaginal device made of PHB-V (Progestar®, Innovare, São Paulo, Brazil), 240 Nellore (Bos indicus) cows, between 3 and 8 years of age, were randomly assigned to 2 experiments. In experiment 1, new P4 devices DIB (control group, DIB1; n = 60) were compared with PHB-V (Progestar1; n = 76); in experiment 2, previously used DIB (DIB2; n = 52) and PHB-V (Progestar2; n = 52) devices were compared. All groups were treated with 2 mg of estradiol benzoate i.m. (Innovare) and received the intravaginal device according to group (Day 0). Together with P4 device withdrawal (Day 8) all animals received 300IU of eCG i.m. (Novormon®, Schering-Plough) and 150 μg i.m. of D-cloprostenol (Croniben®, Biogenesis-Bagó, Buenos Aires, Argentina). On Day 9, all cows were treated with 1 mg of estradiol benzoate and the FTAI was performed 30 h later. The data were submitted to ANOVA (PROC GLM of SAS, SAS Institute, Cary, NC, USA), separating as causes of variance the effect of treatment, postpartum period (PPP1-45 until 70 days and PPP2-71 until 90 days), body condition score (BCS4, BCS5, and BCS6 using a 1 to 9 scale), cyclicity (cycling and noncycling) and clean-up bulls (5 bulls nominated with numbers 1 to 5). Significant differences were considered to have P < 0.05. In experiment 1, interactions were not significant, there was no effect of treatment on FTAI (DIB1: 66.7% v. Progestar1: 65.8%) or breeding season pregnancy rates (BSPR; DIB1: 95.0% v. Progestar1: 94.7%) and no effect of other variables on FTAI pregnancy rates (FTAIPR) and BSPR. In experiment 2 there was no effect of treatment on FTAIPR (DIB2: 61.6% v. Progestar2: 55.8%) and BSPR (DIB2: 94.2% v. Progestar2: 88.5%). As ANOVA indicated a significant effect of clean-up bulls on BSPR, Tukey test was used to split it up. A bull of the Progestar2 group had lower performance (41.7%) causing the difference (albeit nonsignificant) on BSPR. This experiment showed that Progestar and DIB had the same efficiency on pregnancy rate and both treatments have the potential to produce FTAIPR >50% and BSPR >90%. Fapesp-Fundação de Amparo à Pesquisa do Estado de São Paulo. CNPq-Conselho Nacional de Desenvolvimento Científico e Tecnológico.

24 EVALUATION OF THE PREGNANCY RATE OF FIXED-TIME ARTIFICIALLY INSEMINATED BOS INDICUS COWS USING HETEROSPERMIC AND MONOSPERMIC SEMEN

2013 ◽  
Vol 25 (1) ◽  
pp. 159
Author(s):  
J. P. Detomini ◽  
R. R. Tirloni ◽  
C. V. Araujo ◽  
L. R. Martins
Keyword(s):  
Pregnancy Rate ◽  
Estrous Cycle ◽  
Body Condition Score ◽  
Bos Indicus ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Chi Square ◽  
Mato Grosso ◽  
Chi Square Test

The aim of this study was to establish a comparison between heterospermic and monospermic semen on the pregnancy rate of fixed-time AI Bos indicus cows. The experiment was conducted in the state of Mato Grosso, Brazil, during December 2010 and February 2011. Multiparous (n = 173) and primiparous (n = 204) Nelore cows, 45 ± 15 days postpartum, were evaluated on body condition score (BCS; score 1 to 9), and those that presented BCS between 5 and 8 were selected. Two protocols of estrous cycle synchronization were performed (PeCG and Pshang). Synchronization of the estrous cycle was achieved with the aid of the following protocol: on Day 0, all animals were treated with a progesterone vaginal implant with 1.9 g of progesterone combined with an IM injection of 2 mg of estradiol benzoate. On Day 7, an IM injection of 12.5 mg of dinoprost was administered. On Day 9, implants were withdrawn and the animals received an IM injection of 0.5 mg of estradiol cypionate. On Day 9, part of the animals (n = 222) received an IM injection of 300 IU mg of eCG (PeCG) and the other part (n = 155) had their calves removed for 48 hours (Pshang). All animals were inseminated 46 to 52 h after the end of the protocol. Cows were allocated randomly in 4 groups according to the donor male sample in the semen straw and groups were designated as MA (monospermic straw from bull A; n = 110), MB (monospermic straw from bull B; n = 102), MC (monospermic straw from bull C; n = 108), and HT (heterospermic sample from bulls A, B, and C; n = 57). Commercially available semen straws from Aberdeen Angus donors were used (ABS Pecplan, Brazil). Pregnancy diagnosis was performed 35 days after AI using real-time ultrasonography. Results are summarized in Table 1. There was no statistical difference between MA, MB, MC, and HT in any group according to the chi-square test (P > 0.05). However, there was a remarkable difference in pregnancy rates among the 3 bulls tested considering both protocols, probably due to the variation in the ovulation moment. Table 1.Pregnancy rates of Nelore cows submitted to FTAI protocol inseminated with monospermic (bull A, B, or C) or heterospermic (HT) semen

203 EFFECT OF SUPPLEMENTAL FOLLICLE-STIMULATING HORMONE OR EQUINE CHORIONIC GONADOTROPIN DURING THE PROTOCOL FOR TIMED AI IN HIGH-PRODUCING HOLSTEIN COWS

2010 ◽  
Vol 22 (1) ◽  
pp. 260 ◽  
Author(s):  
H. Ayres ◽  
R. M. Ferreira ◽  
M. C. Wiltbank ◽  
P. S. Baruselli
Keyword(s):  
Body Condition Score ◽  
Estradiol Benzoate ◽  
Sao Paulo ◽  
Additional Treatment ◽  
Dairy Herds ◽  
São Paulo ◽  
Condition Score ◽  
Parity Number ◽  
Equine Chorionic Gonadotropin ◽  
Ovulatory Follicles

Programs that allow timed AI (TAI) have been used to improve reproductive efficiency in dairy herds; however, in many cases pregnancies per AI (P/AI) during these programs remain suboptimal. In the present experiment, it was hypothesized that treatment with FSH or eCG at the time of the PGF2 treatment of the TAI protocol would increase fertility in dairy cattle. This experiment was performed in June 2008 (winter) and January 2009 (summer) on 2 commercial dairy farms located in southeast Brazil. On a random day of the estrous cycle (Day 0), 694 cows at 196 ± 138 days in milk (DIM) received 2 mg of estradiol benzoate (RICB®, Tecnopec, São Paulo, Brazil) i.m. and a releasing intravaginal device (P4; Primer®, Tecnopec), which was removed on Day 8 when 150 μg of PGF (Prolise®, ARSA, Buenos Aires, Argentina) i.m. were administered. At this time, the cows were stratified by days in milk (DIM), parity, number of insemination, milk production, presence of a CL on Day 0, and body condition score (BCS) and were randomly assigned to 1 of 3 treatments: control (n = 232), consisting of no additional treatment; eCG (n = 232), consisting of 400 IU of eCG (Folligon®, Intervet, São Paulo, Brazil); and FSH (n = 230), consisting of 20 mg of FSH (Folltropin®, Bioniche, Belleville, Ontario, Canada). All cows received GnRH (100 μg of gonadorelin; Fertagyl®, Intervet) and were TAI 56 h after the removal of the device. Pregnancy diagnosis was performed 30 and 60 days after TAI. Also, a subset of animals (n = 89) was submitted to ultrasonographic exams to evaluate the diameter of the ovulatory follicles (ØOF), time to ovulation after P4 removal (TOV), and ovulation rate (OR). Statistical analyses were performed with logistic regression by PROC GLIMMIX of SAS (SAS Institute, Cary, NC, USA). There were no interactions of treatment with DIM, parity, number of insemination, BCS, locomotion score, sire, batch, inseminator, farm, or month; therefore, these variables were removed from the statistical model. The overall P/AI was different between treatments at 30 days after TAI [control = 28.0% (65/232), eCG = 29.7% (69/232), and FSH = 18.7% (43/230); P = 0.01], but not at 60 days [control = 21.6% (50/232), eCG = 24.1% (56/232), and FSH = 16.1% (37/230); P = 0.08]. Pregnancy loss was also similar among treatments [control = 23.1% (15/65), eCG = 18.8% (13/69), and FSH = 14.0% (6/43); P = 0.08]. In addition, the treatments (control, eCG, and FSH, respectively) were not different among ØOF (13.78 ± 0.52; 14.50 ± 0.59; and 15.35 ± 0.69 mm; P = 0.15); TOV (82.50 ± 2.14; 78.48 ± 2.35; and 78.96 ± 2.39 h after P4 withdrawal; P = 0.30); or OR [82.76% (24/29); 83.33% (25/30); and 83.33% (25/30); P = 0.44]. Thus, these data do not indicate a major fertility-enhancing effect of a single eCG or FSH treatment during protocol for TAI in high-producing dairy cows, contrary to the hypothesis of the present study. Tecnopec, Fazenda Campestre, Agrindus S/A, Echoa e Nutricell Nutrientes Celulares.

16 OVULATION AND PREGNANCY RATES IN POSTPARTUM BOS INDICUS COWS TREATED WITH PROGESTERONE VAGINAL DEVICES AND ESTRADIOL BENZOATE, WITH OR WITHOUT eCG AND TEMPORARY WEANING

2006 ◽  
Vol 18 (2) ◽  
pp. 116 ◽  
Author(s):  
D. Maraña ◽  
L. Cutaia ◽  
L. Peres ◽  
D. Pincinato ◽  
L. F. K. Borges ◽  
...  
Keyword(s):  
Growth Rate ◽  
Body Condition Score ◽  
Bos Indicus ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Corpora Lutea ◽  
Chi Square ◽  
Ovulatory Follicle ◽  
Chi Square Test

Two experiments were designed to compare the effects of eCG treatment and temporary weaning on ovulation and pregnancy rates in postpartum cows. In Experiment 1, 39 lactating multiparous crossbred Bos indicus cows, 60 to 80 d postpartum with a body condition score (BCS) between 2.0 to 2.5 out of 5 were randomly allocated to one of four treatment groups in a 2 � 2 factorial design. On Day 0, all cows received a progesterone (P4) intravaginal device (DIB; Syntex, Argentina) and 2 mg estradiol benzoate (EB) i.m. (Syntex). On Day 8, DIB devices were removed and all cows received 150 �g D(+)cloprostenol i.m. (Ciclase; Syntex) and were randomly divided to receive 400 IU eCG i.m. (Novormon 5000; Syntex) at the same time or no treatment. In addition, half of the cows in each group had their calves weaned temporarily (TW) for 56 h at the time of DIB removal; the other half remained with their calves. All cows received 1 mg EB i.m. on Day 9 and were examined every 8 h by ultrasonography from the time of DIB removal until ovulation. Data were analyzed by ANOVA and chi-square test. The diameter of the dominant follicle on Day 8 (eCG: 7.8 � 0.5 mm vs. no eCG: 8.2 � 0.4 mm, and TW: 7.6 � 0.4 mm vs. no TW 8.4 � 0.5 mm), the proportion of cows that ovulated (eCG: 12/20, 60% vs. no eCG: 9/19, 47%; and TW: 13/20, 65% vs. no TW: 8/19, 42%, and the interval to ovulation (eCG: 72.0 � 1.4 h vs. no eCG: 75.6 � 2.0 h, and TW: 73.8 � 1.6 h vs. no TW: 73.0 � 1.8 h) did not differ among groups (P > 0.05). Although there was no effect of eCG treatment or eCG by TW interaction (P > 0.3) on the size of the preovulatory follicle (eCG: 11.1 � 0.4 mm vs. no eCG: 10.1 � 0.6 mm), it was smaller in cows TW (9.9 � 04 mm), compared to those not TW (11.8 � 0.3 mm; P < 0.05). Nevertheless, the growth rate of the ovulatory follicle was greater (P < 0.02) in cows treated with eCG (1.1 � 0.1 mm/day) than in those not treated with eCG (0.6 � 0.1 mm/day). In Experiment 2 769 lactating multiparous crossbred Bos indicus cows received the same treatments as those in Experiment 1 and were fixed-time inseminated (FTAI) 52 to 56 h after DIB removal. Cows were examined by ultrasonography on Day 0 (22.5% with corpora lutea (CL), 30.0% with follicles >8 mm, and 47.5% with follicles <8 mm) and 42 d after FTAI to determine pregnancy. Data were analyzed by logistic regression. Pregnancy rates were not affected (P > 0.7) by TW (TW: 141/397, 37.2% vs. no TW: 141/390, 36.1%) or the TW by eCG interaction (P > 0.7). However, pregnancy rates were higher (P < 0.05) in cows treated with eCG (154/377, 40.8%) than in those not treated with eCG (128/392, 32.6%). The use of eCG but not TW improved pregnancy rates following FTAI in postpartum Bos indicus cows. Results also suggest that the eCG-related increase in pregnancy rates may be due to the final growth rate of the ovulatory follicle.

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