scholarly journals From The Cover: The host response to smallpox: Analysis of the gene expression program in peripheral blood cells in a nonhuman primate model

2004 ◽  
Vol 101 (42) ◽  
pp. 15190-15195 ◽  
Author(s):  
K. H. Rubins ◽  
L. E. Hensley ◽  
P. B. Jahrling ◽  
A. R. Whitney ◽  
T. W. Geisbert ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nonhuman Primate ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Host Response ◽  
Gene Expression Program ◽  
Peripheral Blood Cells ◽  
Nonhuman Primate Model ◽  
Primate Model ◽  
Expression Program

scholarly journals Transcriptomic phases of periodontitis lesions using the nonhuman primate model

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jeffrey L. Ebersole ◽  
Radhakrishnan Nagarajan ◽  
Sreenatha Kirakodu ◽  
Octavio A. Gonzalez
Keyword(s):  
Gene Expression ◽  
Nonhuman Primate ◽  
Gingival Crevicular Fluid ◽  
Inflammatory Responses ◽  
Expression Profiles ◽  
Lesion Detection ◽  
Gingival Tissue ◽  
Specific Gene ◽  
Nonhuman Primate Model ◽  
Primate Model

AbstractWe used a nonhuman primate model of ligature-induced periodontitis to identify patterns of gingival transcriptomic after changes demarcating phases of periodontitis lesions (initiation, progression, resolution). A total of 18 adult Macaca mulatta (12–22 years) had ligatures placed (premolar, 1st molar teeth) in all 4 quadrants. Gingival tissue samples were obtained (baseline, 2 weeks, 1 and 3 months during periodontitis and at 5 months resolution). Gene expression was analyzed by microarray [Rhesus Gene 1.0 ST Array (Affymetrix)]. Compared to baseline, a large array of genes were significantly altered at initiation (n = 6049), early progression (n = 4893), and late progression (n = 5078) of disease, with the preponderance being up-regulated. Additionally, 1918 genes were altered in expression with disease resolution, skewed towards down-regulation. Assessment of the genes demonstrated specific profiles of epithelial, bone/connective tissue, apoptosis/autophagy, metabolism, regulatory, immune, and inflammatory responses that were related to health, stages of disease, and tissues with resolved lesions. Unique transcriptomic profiles occured during the kinetics of the periodontitis lesion exacerbation and remission. We delineated phase specific gene expression profiles of the disease lesion. Detection of these gene products in gingival crevicular fluid samples from human disease may contribute to a better understanding of the biological dynamics of the disease to improve patient management.

scholarly journals RNA Sequencing of Human Peripheral Blood Cells Indicates Upregulation of Immune-Related Genes in Huntington's Disease

2020 ◽  
Vol 11 ◽  
Author(s):  
Miguel A. Andrade-Navarro ◽  
Katja Mühlenberg ◽  
Eike J. Spruth ◽  
Nancy Mah ◽  
Adrián González-López ◽  
...  
Keyword(s):  
Gene Expression ◽  
Huntington's Disease ◽  
Huntington’S Disease ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Trinucleotide Repeat ◽  
Neurodegenerative Disorder ◽  
Gene Expression Signature ◽  
Interferon Response ◽  
Peripheral Blood Cells

Huntington's disease (HD) is an autosomal dominantly inherited neurodegenerative disorder caused by a trinucleotide repeat expansion in the Huntingtin gene. As disease-modifying therapies for HD are being developed, peripheral blood cells may be used to indicate disease progression and to monitor treatment response. In order to investigate whether gene expression changes can be found in the blood of individuals with HD that distinguish them from healthy controls, we performed transcriptome analysis by next-generation sequencing (RNA-seq). We detected a gene expression signature consistent with dysregulation of immune-related functions and inflammatory response in peripheral blood from HD cases vs. controls, including induction of the interferon response genes, IFITM3, IFI6 and IRF7. Our results suggest that it is possible to detect gene expression changes in blood samples from individuals with HD, which may reflect the immune pathology associated with the disease.

Gene Expression Analysis in Human Peripheral Blood Cells after 900 MHz RF-EMF Short-Term Exposure

2018 ◽  
Vol 189 (5) ◽  
pp. 529-540 ◽  
Author(s):  
Andreas Lamkowski ◽  
Matthias Kreitlow ◽  
Jörg Radunz ◽  
Martin Willenbockel ◽  
Frank Sabath ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Gene Expression Analysis ◽  
Human Peripheral Blood ◽  
Peripheral Blood Cells ◽  
Short Term ◽  
Short Term Exposure

Comparison of Plasma Cell-Free DNA Levels with Gene Expression Profiles of Peripheral Blood Cells During Haemodialysis

2010 ◽  
pp. 159-163
Author(s):  
Ales Horinek ◽  
Ales Panczak ◽  
Magdalena Mokrejsova ◽  
Katarina Rocinova ◽  
Marie Korabecna ◽  
...  
Keyword(s):  
Gene Expression ◽  
Plasma Cell ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Peripheral Blood Cells ◽  
Cell Free Dna ◽  
Free Dna

Gene expression signature in peripheral blood cells from medical students exposed to chronic psychological stress

2007 ◽  
Vol 76 (3) ◽  
pp. 147-155 ◽  
Author(s):  
Tomoko Kawai ◽  
Kyoko Morita ◽  
Kiyoshi Masuda ◽  
Kensei Nishida ◽  
Michiyo Shikishima ◽  
...  
Keyword(s):  
Gene Expression ◽  
Medical Students ◽  
Psychological Stress ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Gene Expression Signature ◽  
Peripheral Blood Cells ◽  
Expression Signature

Abstract 12559: Rapid Atrial Pacing Augments Fibrinogen Expression With Activation of IL-6/STAT3 Signaling Pathway in the Rat Liver

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Takanori Yaegashi ◽  
Takeshi Kato ◽  
Soichiro Usui ◽  
Naomi Kanamori ◽  
Hiroshi Furusho ◽  
...  
Keyword(s):  
Gene Expression ◽  
Signaling Pathway ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Atrial Pacing ◽  
Peripheral Blood Cells ◽  
Stat3 Signaling ◽  
Stat3 Signaling Pathway ◽  
Stat3 Phosphorylation ◽  
Atrial Excitation

Introduction: Atrial fibrillation (AF) activates coagulation system leading to hypercoagulation of the blood. However, it is still unknown whether rapid atrial excitation per se affects gene expression remotely in the liver, the major source of coagulation factors and other prothrombotic molecules. Methods and Results: The AF model was created by rapid atrial pacing at the frequency of 1200 bpm in anesthetized 10-week-old Sprague-Dawley rats. The livers and peripheral blood cells were collected and analyzed after the pacing of 12 hours. Sham-operated rats underwent the identical procedure without electrical stimulation. DNA microarray revealed marked changes in hepatic gene expression after 12 hours atrial pacing. Hierarchical clustering with 13871 filtered genes or genes related to coagulation including fibrinogen, demonstrated clusters for the pacing or sham. The quantitative RT-PCR focused on prothrombotic molecules revealed that rapid atrial pacing significantly augmented the hepatic mRNA expressions of fibrinogen α, β, γ-chain, prothrombin, antithrombin-III, plasminogen, and coagulation factor X. The increase of fibrinogen protein in the liver was also confirmed by Western blotting (Figure A). We further investigated the mechanism of enhanced fibrinogen production and identified increased IL-6 mRNA expression in the peripheral blood cells by rapid atrial pacing (Figure B). IL-6 was also prominent in CD11b positive cells infiltrated in the liver, and possibly promoted STAT3 phosphorylation in the nuclei of hepatocytes (Figure C). Conclusions: The rapid atrial excitation mimicking paroxysmal AF altered the hepatic gene expressions of prothrombotic molecules. Increased fibrinogen expression in the liver was accompanied by activation of IL-6/STAT3 signaling pathway in the peripheral blood and the liver. These findings might imply the cardio-hepatic interaction in AF and provide new insight into the prevention of AF-related thromboembolism.

scholarly journals Gene Expression Profiling in Peripheral Blood Cells and Synovial Membranes of Patients with Psoriatic Arthritis

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0128262 ◽  
Author(s):  
Marzia Dolcino ◽  
Andrea Ottria ◽  
Alessandro Barbieri ◽  
Giuseppe Patuzzo ◽  
Elisa Tinazzi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Psoriatic Arthritis ◽  
Gene Expression Profiling ◽  
Peripheral Blood ◽  
Expression Profiling ◽  
Blood Cells ◽  
Peripheral Blood Cells ◽  
Synovial Membranes

scholarly journals Gene expression profiling of peripheral blood cells: new insights into Ewing sarcoma biology and clinical applications

2014 ◽  
Vol 31 (8) ◽  
Author(s):  
Joanna Przybyl ◽  
Katarzyna Kozak ◽  
Hanna Kosela ◽  
Slawomir Falkowski ◽  
Tomasz Switaj ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Ewing Sarcoma ◽  
Peripheral Blood ◽  
Expression Profiling ◽  
Blood Cells ◽  
Clinical Applications ◽  
Peripheral Blood Cells
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