scholarly journals Coordinated bacterial and plant sulfur metabolism in Enterobacter sp. SA187–induced plant salt stress tolerance

2021 ◽  
Vol 118 (46) ◽  
pp. e2107417118
Author(s):  
Cristina Andrés-Barrao ◽  
Hanin Alzubaidy ◽  
Rewaa Jalal ◽  
Kiruthiga G. Mariappan ◽  
Axel de Zélicourt ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Tolerance ◽  
Sulfur Metabolism ◽  
Endophytic Bacterium ◽  
Growth And Yield ◽  
Common Mechanism ◽  
Salt Stress Tolerance ◽  
Bacterial Gene ◽  
Bacterial Gene Expression ◽  
Flagellar Biosynthesis

Enterobacter sp. SA187 is a root endophytic bacterium that maintains growth and yield of plants under abiotic stress conditions. In this work, we compared the metabolic wirings of Arabidopsis and SA187 in the free-living and endophytic interaction states. The interaction of SA187 with Arabidopsis induced massive changes in bacterial gene expression for chemotaxis, flagellar biosynthesis, quorum sensing, and biofilm formation. Besides modification of the bacterial carbon and energy metabolism, various nutrient and metabolite transporters and the entire sulfur pathway were up-regulated. Under salt stress, Arabidopsis resembled plants under sulfate starvation but not when colonized by SA187, which reprogramed the sulfur regulon of Arabidopsis. In accordance, salt hypersensitivity of multiple Arabidopsis sulfur metabolism mutants was partially or completely rescued by SA187 as much as by the addition of sulfate, L-cysteine, or L-methionine. Many components of the sulfur metabolism that are localized in the chloroplast were partially rescued by SA187. Finally, salt-induced accumulation of reactive oxygen species as well as the hypersensitivity of LSU mutants were suppressed by SA187. LSUs encode a central regulator linking sulfur metabolism to chloroplast superoxide dismutase activity. The coordinated regulation of the sulfur metabolic pathways in both the beneficial microorganism and the host plant is required for salt stress tolerance in Arabidopsis and might be a common mechanism utilized by different beneficial microbes to mitigate the harmful effects of different abiotic stresses on plants.

scholarly journals The Potentiality of Marine Macro-Algae as Bio-Fertilizers to Improve the Productivity and Salt Stress Tolerance of Canola (Brassica napus L.) Plants

Agronomy ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 146 ◽  
Author(s):  
Hashem H.A. ◽  
Mansour H.A. ◽  
El-Khawas S.A. ◽  
Hassanein R.A.
Keyword(s):  
Salt Stress ◽  
Brassica Napus ◽  
Stress Tolerance ◽  
Growth And Yield ◽  
Phytochemical Analysis ◽  
Salt Stress Tolerance ◽  
Brassica Napus L ◽  
Indole Butyric Acid ◽  
Total Carbohydrates ◽  
The Impact

The present study aimed to evaluate the potentiality of three seaweeds, which belong to different algal taxa (green alga Ulva lactuca Linnaeus, brown alga Cystoseira spp., and red alga Gelidium crinale (Hare ex Turner) Gaillon) as bio-fertilizers to improve the growth and yield of canola (Brassica napus L.) plants under greenhouse conditions. Furthermore, the impact of seaweeds in alleviating the effects of salt stress (75 and 150 mM NaCl) on canola plants was also investigated. The three examined seaweeds (applied as soil amendments) successfully alleviated the harmful effects of salinity on canola plants by significantly reducing the inhibition of chlorophyll a, b, total carbohydrate accumulation, and growth promoting hormones, while increasing antioxidative compounds, such as phenols, flavonoids, anthocyanin, and osmoprotectants, including total carbohydrates and proline. Phytochemical analysis of the three examined seaweeds suggests that their stimulatory effect on growth and productivity under normal and salinity growth conditions may be linked to their constituents of a wide variety of growth promotive hormones, including indole acetic acid, indole butyric acid, gibberellic acid, cytokinins, total carbohydrates, and phenolic compounds. U. lactuca was found to be the best candidate to be used as a bio-fertilizer to improve canola growth, yield, and salt stress tolerance.

scholarly journals Transcriptome Changes Reveal the Molecular Mechanisms of Humic Acid-Induced Salt Stress Tolerance in Arabidopsis

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 782
Author(s):  
Joon-Yung Cha ◽  
Sang-Ho Kang ◽  
Myung Geun Ji ◽  
Gyeong-Im Shin ◽  
Song Yi Jeong ◽  
...  
Keyword(s):  
Salt Stress ◽  
Abiotic Stress ◽  
Humic Acid ◽  
Stress Tolerance ◽  
Plant Development ◽  
Molecular Mechanisms ◽  
Abiotic Stress Tolerance ◽  
Principal Component ◽  
Salt Stress Tolerance ◽  
Genes Encoding

Humic acid (HA) is a principal component of humic substances, which make up the complex organic matter that broadly exists in soil environments. HA promotes plant development as well as stress tolerance, however the precise molecular mechanism for these is little known. Here we conducted transcriptome analysis to elucidate the molecular mechanisms by which HA enhances salt stress tolerance. Gene Ontology Enrichment Analysis pointed to the involvement of diverse abiotic stress-related genes encoding HEAT-SHOCK PROTEINs and redox proteins, which were up-regulated by HA regardless of salt stress. Genes related to biotic stress and secondary metabolic process were mainly down-regulated by HA. In addition, HA up-regulated genes encoding transcription factors (TFs) involved in plant development as well as abiotic stress tolerance, and down-regulated TF genes involved in secondary metabolic processes. Our transcriptome information provided here provides molecular evidences and improves our understanding of how HA confers tolerance to salinity stress in plants.

scholarly journals Expression Analyses of Soybean VOZ Transcription Factors and the Role of GmVOZ1G in Drought and Salt Stress Tolerance

2020 ◽  
Vol 21 (6) ◽  
pp. 2177 ◽  
Author(s):  
Bo Li ◽  
Jia-Cheng Zheng ◽  
Ting-Ting Wang ◽  
Dong-Hong Min ◽  
Wen-Liang Wei ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Salt Stress ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Zinc Finger ◽  
Hairy Roots ◽  
Yeast Cells ◽  
Salt Stress Tolerance ◽  
Drought And Salt Stress

Vascular plant one-zinc-finger (VOZ) transcription factor, a plant specific one-zinc-finger-type transcriptional activator, is involved in regulating numerous biological processes such as floral induction and development, defense against pathogens, and response to multiple types of abiotic stress. Six VOZ transcription factor-encoding genes (GmVOZs) have been reported to exist in the soybean (Glycine max) genome. In spite of this, little information is currently available regarding GmVOZs. In this study, GmVOZs were cloned and characterized. GmVOZ genes encode proteins possessing transcriptional activation activity in yeast cells. GmVOZ1E, GmVOZ2B, and GmVOZ2D gene products were widely dispersed in the cytosol, while GmVOZ1G was primarily located in the nucleus. GmVOZs displayed a differential expression profile under dehydration, salt, and salicylic acid (SA) stress conditions. Among them, GmVOZ1G showed a significantly induced expression in response to all stress treatments. Overexpression of GmVOZ1G in soybean hairy roots resulted in a greater tolerance to drought and salt stress. In contrast, RNA interference (RNAi) soybean hairy roots suppressing GmVOZ1G were more sensitive to both of these stresses. Under drought treatment, soybean composite plants with an overexpression of hairy roots had higher relative water content (RWC). In response to drought and salt stress, lower malondialdehyde (MDA) accumulation and higher peroxidase (POD) and superoxide dismutase (SOD) activities were observed in soybean composite seedlings with an overexpression of hairy roots. The opposite results for each physiological parameter were obtained in RNAi lines. In conclusion, GmVOZ1G positively regulates drought and salt stress tolerance in soybean hairy roots. Our results will be valuable for the functional characterization of soybean VOZ transcription factors under abiotic stress.

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