Antisense RNA inhibits endogenous gene expression in mouse preimplantation embryos: lack of double-stranded RNA "melting" activity.

AbstractPaternal obesity is known to have a negative impact on the male’s reproductive health as well as the health of his offspring. Although epigenetic mechanisms have been implicated in the non-genetic transmission of acquired traits, the effect of paternal obesity on gene expression in the preimplantation embryo has not been fully studied. To this end, we investigated whether paternal obesity is associated with gene expression changes in eight-cell stage embryos fathered by males on a high-fat diet. We used single embryo RNA-seq to compare the gene expression profile of embryos generated by males on a high fat (HFD) versus control (CD) diet. This analysis revealed significant upregulation of the Samd4b and Gata6 gene in embryos in response to a paternal HFD. Furthermore, we could show a significant increase in expression of both Gata6 and Samd4b during differentiation of stromal vascular cells into mature adipocytes. These findings suggest that paternal obesity may induce changes in the male germ cells which are associated with the gene expression changes in the resulting preimplantation embryos.

Download Full-text

The [KIL-d] Element Specifically Regulates Viral Gene Expression in Yeast

Genetics ◽

10.1093/genetics/155.2.601 ◽

2000 ◽

Vol 155 (2) ◽

pp. 601-609 ◽

Cited By ~ 1

Author(s):

Zsolt Tallóczy ◽

Rebecca Mazar ◽

Denise E Georgopoulos ◽

Fausto Ramos ◽

Michael J Leibowitz

Keyword(s):

Gene Expression ◽

Phenotypic Expression ◽

Viral Gene Expression ◽

Virus Genome ◽

Viral Rna ◽

High Rate ◽

Viral Gene ◽

Double Stranded Rna ◽

Yeast Prions ◽

Killer Virus

Abstract The cytoplasmically inherited [KIL-d] element epigenetically regulates killer virus gene expression in Saccharomyces cerevisiae. [KIL-d] results in variegated defects in expression of the M double-stranded RNA viral segment in haploid cells that are “healed” in diploids. We report that the [KIL-d] element is spontaneously lost with a frequency of 10−4–10−5 and reappears with variegated phenotypic expression with a frequency of ≥10−3. This high rate of loss and higher rate of reappearance is unlike any known nucleic acid replicon but resembles the behavior of yeast prions. However, [KIL-d] is distinct from the known yeast prions in its relative guanidinium hydrochloride incurability and independence of Hsp104 protein for its maintenance. Despite its transmissibility by successive cytoplasmic transfers, multiple cytoplasmic nucleic acids have been proven not to carry the [KIL-d] trait. [KIL-d] epigenetically regulates the expression of the M double-stranded RNA satellite virus genome, but fails to alter the expression of M cDNA. This specificity remained even after a cycle of mating and meiosis. Due to its unique genetic properties and viral RNA specificity, [KIL-d] represents a new type of genetic element that interacts with a viral RNA genome.

Download Full-text

Enhancement of interferon-gamma-induced major histocompatibility complex class II gene expression by expressing an antisense RNA of poly(ADP-ribose) synthetase.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)37495-1 ◽

1994 ◽

Vol 269 (8) ◽

pp. 5543-5547

Author(s):

Z. Qu ◽

S. Fujimoto ◽

T. Taniguchi

Keyword(s):

Gene Expression ◽

Major Histocompatibility Complex ◽

Major Histocompatibility Complex Class ◽

Interferon Gamma ◽

Antisense Rna ◽

Class Ii ◽

Complex Class ◽

Major Histocompatibility ◽

Histocompatibility Complex

Download Full-text

636. SOD3 Gene Transfer Enhances Tissue Healing by Regulating Endogenous Gene Expression

Molecular Therapy ◽

10.1016/s1525-0016(16)40039-0 ◽

2008 ◽

Vol 16 ◽

pp. S238

Keyword(s):

Gene Expression ◽

Gene Transfer ◽

Endogenous Gene ◽

Tissue Healing

Download Full-text

Double-stranded RNA induces S100 gene expression by a cycloheximide-sensitive factor

FEBS Letters ◽

10.1016/j.febslet.2011.12.022 ◽

2011 ◽

Vol 586 (2) ◽

pp. 196-203 ◽

Cited By ~ 10

Author(s):

Andreas Voss ◽

Kirsten Gescher ◽

Andreas Hensel ◽

Wolfgang Nacken ◽

Kurt S. Zänker ◽

...

Keyword(s):

Gene Expression ◽

Double Stranded Rna ◽

Sensitive Factor ◽

S100 Gene

Download Full-text

Effects of embryo culture on global pattern of gene expression in preimplantation mouse embryos

Reproduction ◽

10.1530/rep.1.00297 ◽

2004 ◽

Vol 128 (3) ◽

pp. 301-311 ◽

Cited By ~ 193

Author(s):

Paolo Rinaudo ◽

Richard M Schultz

Keyword(s):

Gene Expression ◽

Preimplantation Embryos ◽

Expression Analysis Systematic Explorer ◽

Cell Stage ◽

Global Pattern ◽

Preimplantation Mouse Embryos ◽

Preimplantation Mouse ◽

Global Patterns ◽

The One

Culture of preimplantation embryos affects gene expression. The magnitude of the effect on the global pattern of gene expression, however, is not known. We compared global patterns of gene expression in blastocysts cultured from the one-cell stage in either Whitten’s medium or KSOM + amino acids (KSOM/AA) with that of blastocysts that developed in vivo, using the Affymetrix MOE430A chip. The analysis revealed that expression of 114 genes was affected after culture in Whitten’s medium, whereas only 29 genes were mis-expressed after culture in KSOM/AA. Expression Analysis Systematic Explorer was used to identify biological and molecular processes that are perturbed after culture and indicated that genes involved in protein synthesis, cell proliferation and transporter function were down-regulated after culture in Whitten’s medium. A common set of genes involved in transporter function was also down-regulated after culture in KSOM/AA. These results provide insights as to why embryos develop better in KSOM/AA than in Whitten’s medium, and highlight the power of microarray analysis to assess global patterns of gene expression.

Download Full-text