scholarly journals Characterization of a Stem-like Subpopulation in Basal-like Ductal Carcinoma in Situ (DCIS) Lesions

2013 ◽  
Vol 289 (3) ◽  
pp. 1303-1312 ◽  
Author(s):  
Qinglin Li ◽  
Gabriel Eades ◽  
Yuan Yao ◽  
Yongshu Zhang ◽  
Qun Zhou
Keyword(s):  
Cancer Cells ◽  
Aldehyde Dehydrogenase ◽  
Breast Cancer Cells ◽  
Ductal Carcinoma ◽  
Chemopreventive Agent ◽  
Aldehyde Dehydrogenase 1 ◽  
Aldh1 Expression ◽  
Migration Potential

Previously, we found that basal-like ductal carcinoma in situ (DCIS) contains cancer stem-like cells. Here, we characterize stem-like subpopulations in a model of basal-like DCIS and identify subpopulations of CD49f+/CD24− stem-like cells that possess aldehyde dehydrogenase 1 activity. We found that these cells show enhanced migration potential compared with non-stem DCIS cells. We also found that the chemopreventive agent sulforaphane can target these DCIS stem-like cells, reduce aldehyde dehydrogenase 1 (ALDH1) expression, and decrease mammosphere and progenitor colony formation. Furthermore, we characterized exosomal trafficking of microRNAs in DCIS and found that several microRNAs (miRs) including miR-140, miR-29a, and miR-21 are differentially expressed in exosomes from DCIS stem-like cells. We found that SFN treatment could reprogram DCIS stem-like cells as evidenced by significant changes in exosomal secretion more closely resembling that of non-stem cancer cells. Finally, we demonstrated that exosomal secretion of miR-140 might impact signaling in nearby breast cancer cells.

scholarly journals Characterization of aldehyde dehydrogenase 1 high ovarian cancer cells: Towards targeted stem cell therapy

2016 ◽  
Vol 142 (2) ◽  
pp. 341-348 ◽  
Author(s):  
Allison C. Sharrow ◽  
Brandy Perkins ◽  
Michael I. Collector ◽  
Wayne Yu ◽  
Brian W. Simons ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Stem Cell ◽  
Cell Therapy ◽  
Cancer Cells ◽  
Aldehyde Dehydrogenase ◽  
Stem Cell Therapy ◽  
Ovarian Cancer Cells ◽  
Aldehyde Dehydrogenase 1

scholarly journals Synthesis and characterization of novel protein nanodots as drug delivery carriers with an enhanced biological efficacy of melatonin in breast cancer cells

RSC Advances ◽  
2021 ◽  
Vol 11 (16) ◽  
pp. 9076-9085
Author(s):  
Kanchan Yadav ◽  
Megha Das ◽  
Nurul Hassan ◽  
Archana Mishra ◽  
Jayeeta Lahiri ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Drug Delivery ◽  
Cancer Cells ◽  
Live Cell Imaging ◽  
Biomedical Applications ◽  
Breast Cancer Cells ◽  
Cell Imaging ◽  
Synthesis And Characterization ◽  
Novel Protein

A novel nanodot-using protein has been synthesized for the live cell imaging and drug delivery of melatonin in breast cancer cells. Its unique properties hold potential for various biomedical applications in the field of bioimaging and drug delivery.

scholarly journals Analysis of EGFR and HER-2 expressions in ductal carcinomas in situ in canine mammary glands

2014 ◽  
Vol 66 (3) ◽  
pp. 763-768 ◽  
Author(s):  
I.L.D. Silva ◽  
A.P.M. Dias ◽  
A.C. Bertagnolli ◽  
G.D. Cassali ◽  
E. Ferreira
Keyword(s):  
Ductal Carcinoma ◽  
Mammary Glands ◽  
Statistical Correlation ◽  
Low Grade ◽  
Neoplastic Progression ◽  
Her 2 ◽  
Epidermal Growth ◽  
Ductal Carcinomas

Biomolecular evidence has shown that ductal carcinoma in situ(DCIS) may develop into invasive carcinoma of the canine mammary gland, and mutations in proto-oncogenes HER2 and EGFR; two members of the family of epidermal growth factor receptors, may be involved in this process. The purpose of this study was the characterization of the immunohistochemical expression of the EGFR and HER2 proteins in the process of neoplastic transformation, supposedly present in ductal carcinomas in situin canine mammary glands. Fifteen cases of DCIS were evaluated, with a higher expression of HER2 and EGFR being observed in low-grade carcinomas when compared with high-grade neoplasms, and with a high positive statistical correlation in the latter. Results suggest that aggressive tumors tend to lose the expression of EGFR and HER2 simultaneously. The loss of the expression of these markers may be related to the process of neoplastic progression in canine mammary tumors.

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