Down-regulation ofCdc6, a Cell Cycle Regulatory Gene, in Prostate Cancer

Prostate cancer is the most common cancer in Western countries. Recently, Asian countries are being affected by Western habits, which have had an important role in the rapid increase in cancer incidence. Sanggenol L (San L) is a natural flavonoid present in the root barks of Morus alba, which induces anti-cancer activities in ovarian cancer cells. However, the molecular and cellular mechanisms of the effects of sanggenol L on human prostate cancer cells have not been elucidated. In this study, we investigated whether sanggenol L exerts anti-cancer activity in human prostate cancer cells via apoptosis and cell cycle arrest. Sanggenol L induced caspase-dependent apoptosis (up-regulation of PARP and Bax or down-regulation of procaspase-3, -8, -9, Bid, and Bcl-2), induction of caspase-independent apoptosis (up-regulation of AIF and Endo G on cytosol), suppression of cell cycle (down-regulation of CDK1/2, CDK4, CDK6, cyclin D1, cyclin E, cyclin A, and cyclin B1 or up-regulation of p53 and p21), and inhibition of PI3K/Akt/mTOR signaling (down-regulation of PI3K, p-Akt, and p-mTOR) in prostate cancer cells. These results suggest the induction of apoptosis via suppression of PI3K/Akt/mTOR signaling and cell cycle arrest via activation of p53 in response to sanggenol L in prostate cancer cells.

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PO-0963: Mitochondrial function of the prognostic cell cycle regulatory gene CKS2 in cervical cancer

Radiotherapy and Oncology ◽

10.1016/s0167-8140(15)31081-1 ◽

2014 ◽

Vol 111 ◽

pp. S132

Author(s):

M. Jonsson ◽

C.H. Julin ◽

E.K. Aarnes ◽

G.B. Kristensen ◽

R. Holm ◽

...

Keyword(s):

Cell Cycle ◽

Cervical Cancer ◽

Mitochondrial Function ◽

Regulatory Gene ◽

Cell Cycle Regulatory Gene

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Immunohistochemical Analysis of Cell Cycle Regulatory Gene Products in Normal Trophoblast and Placental Site Trophoblastic Tumor

International Journal of Gynecological Pathology ◽

10.1097/00004347-199807000-00007 ◽

1998 ◽

Vol 17 (3) ◽

pp. 235-240 ◽

Cited By ~ 31

Author(s):

Naoko Ichikawa ◽

Ya-Li Zhai ◽

Tanri Shiozawa ◽

Toshihiko Toki ◽

Hiroshi Noguchi ◽

...

Keyword(s):

Cell Cycle ◽

Regulatory Gene ◽

Immunohistochemical Analysis ◽

Gene Products ◽

Placental Site Trophoblastic Tumor ◽

Trophoblastic Tumor ◽

Placental Site ◽

Cell Cycle Regulatory Gene

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Expressions of cell-cycle regulatory gene products in conventional gastric adenomas: Possible immunohistochemical markers of malignant transformation

Human Pathology ◽

10.1016/s0046-8177(00)80239-7 ◽

2000 ◽

Vol 31 (3) ◽

pp. 279-287 ◽

Cited By ~ 13

Author(s):

M OYA ◽

T YAO ◽

M TSUNEYOSHI

Keyword(s):

Cell Cycle ◽

Malignant Transformation ◽

Regulatory Gene ◽

Gene Products ◽

Immunohistochemical Markers ◽

Cell Cycle Regulatory Gene

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Evidence for a new kind of regulatory gene controlling expression of genes for morphogenesis during the cell cycle in Ustilago violacea

Genetics Research ◽

10.1017/s0016672300015688 ◽

1975 ◽

Vol 25 (3) ◽

pp. 253-266 ◽

Cited By ~ 1

Author(s):

A. W. Day ◽

J. E. Cummins

Keyword(s):

Cell Cycle ◽

Mating Type ◽

Cell Cycle Control ◽

Regulatory Gene ◽

Type Locus ◽

Ustilago Violacea ◽

Temporal Properties ◽

Expression Of Genes ◽

Separate Control ◽

A Cell

SUMMARYThe first part of the paper provides strong supportive evidence for the previous findings (Cummins & Day, 1973; Day & Cummins, 1973) that the two alleles of the mating-type locus of the basidiomycete Ustilago violacea have different periods of inducibility during a cell cycle, and that the cell cycle characteristics of each allele are maintained in freshly isolated diploids. This difference in temporal properties of the alleles appears to be the basis of the dominance of allele a2 as it is inducible during a phase of the cell cycle when allele a1 is non-inducible. During G1 both alleles appear to be inducible and apparently ‘neutralize’ each other so that the cell cannot mate.The second part of the paper provides evidence for a unique genetic control mechanism. The evidence suggests that the period of cell cycle inducibility of a locus governing a morphogenetic pathway may be regulated by a separate control gene the cc locus, with two known alleles ccstr(a stringent or restricted period of inducibility) and ccrel (a relaxed or non-restricted period of inducibility). This hypothesis stems from analysis of a diploid that was a1· ccstr/a2· ccrel and showed dominance of allele a2 during the S and G2 phases when freshly isolated, but which became incapable of mating after a period of subculturing. Analysis of haploids derived from this diploid strain showed that both mating-type alleles were functional but that it was now homozygous for ccstr, i.e. of genotype a1· ccstr/a2·ccstr· Thus the temporal and functional aspects of the mating type alleles are determined by different loci. It is postulated that cell cycle control loci may be widespread and serve to regulate the action of genes concerned with morphogenesis in relation to other cell cycle events.

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