scholarly journals An Alternative Splice Form of Mdm2 Induces p53-independent Cell Growth and Tumorigenesis

2003 ◽  
Vol 279 (6) ◽  
pp. 4877-4886 ◽  
Author(s):  
Heather A. Steinman ◽  
Ezra Burstein ◽  
Christopher Lengner ◽  
Joseph Gosselin ◽  
German Pihan ◽  
...  
Keyword(s):  
Cell Growth ◽  
Alternative Splice ◽  
Splice Form ◽  
Alternative Splice Form

scholarly journals Specificity of Alternative Splice Form Detection Using RT-PCR with a Primer Spanning the Exon Junction

BioTechniques ◽  
2003 ◽  
Vol 34 (6) ◽  
pp. 1244-1249 ◽  
Author(s):  
Natalia Shulzhenko ◽  
Anna S. Smirnova ◽  
Andrey Morgun ◽  
Maria Gerbase-DeLima
Keyword(s):  
Alternative Splice ◽  
Splice Form ◽  
Alternative Splice Form ◽  
Rt Pcr ◽  
Exon Junction

An alternative splice form of CMTM8 induces apoptosis

2007 ◽  
Vol 39 (11) ◽  
pp. 2107-2119 ◽  
Author(s):  
Dan Li ◽  
Caining Jin ◽  
Caihua Yin ◽  
Yingmei Zhang ◽  
Bo Pang ◽  
...  
Keyword(s):  
Alternative Splice ◽  
Splice Form ◽  
Alternative Splice Form

scholarly journals Cloning and Functional Analysis of Hypothalamic Homeobox Gene Bsx1a and Its Isoform, Bsx1b

2007 ◽  
Vol 27 (10) ◽  
pp. 3743-3749 ◽  
Author(s):  
Hui-Yi Chu ◽  
Akihira Ohtoshi
Keyword(s):  
Rna Splicing ◽  
Molecular Mechanisms ◽  
Homeobox Gene ◽  
Dna Binding Protein ◽  
Terminal Region ◽  
Splice Form ◽  
Alternative Splice Form ◽  
Transcriptional Reporter ◽  
Reporter Assays ◽  
Immunohistochemical Analyses

ABSTRACT The hypothalamus is a key regulatory unit of the neuroendocrine system and plays an essential role in energy balance and reproduction. Despite its important role, the molecular mechanisms underlying hypothalamic development are not fully understood. Here, we report molecular analyses of a newly identified murine homeobox gene, Bsx/Bsx1a, that is expressed in the developing and postnatal hypothalamus. We demonstrate that BSX1A is a DNA binding protein and a transcriptional activator. Transcriptional reporter assays identified the C-terminal region of BSX1A as an activation domain. We have isolated an alternative splice form of Bsx1a, designated Bsx1b, which retains the N-terminal region but lacks the homeodomain. Analyses of subcellular localization using transfected cell lines revealed that BSX1A and BSX1B localize in the nuclei and cytoplasm, respectively. Immunohistochemical analyses suggested that both BSX1A and BSX1B are expressed in the neonatal hypothalamus. Taking these data together, we propose that alternative RNA splicing is involved in hypothalamic development/function.

scholarly journals BRS1 mediates plant redox regulation and cold responses

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Dongzhi Zhang ◽  
Yuqian Zhao ◽  
Junzhe Wang ◽  
Peng Zhao ◽  
Shengbao Xu
Keyword(s):  
Stress Responses ◽  
Redox Regulation ◽  
Splice Form ◽  
Alternative Splice Form ◽  
Serine Carboxypeptidase ◽  
Over Expression ◽  
Brassinosteroid Signaling ◽  
Cold Responses ◽  
Precise Role

Abstract Background Brassinosteroid-insensitive 1 suppressor 1 (BRS1) is a serine carboxypeptidase that mediates brassinosteroid signaling and participates in multiple developmental processes in Arabidopsis. However, little is known about the precise role of BRS1 in this context. Results In this study, we analyzed transcriptional and proteomic profiles of Arabidopsis seedlings overexpressing BRS1 and found that this gene was involved in both cold stress responses and redox regulation. Further proteomic evidence showed that BRS1 regulated cell redox by indirectly interacting with cytosolic NADP + -dependent isocitrate dehydrogenase (cICDH). One novel alternative splice form of BRS1 was identified in over-expression mutants brs1-1D, which may confer a new role in plant development and stress responses. Conclusions This study highlights the role of BRS1 in plant redox regulation and stress responses, which extends our understanding of extracellular serine carboxypeptidases.

Electron Microscopic Study of the Epithelium of the Seminal Vesicle of Aging Rats

1974 ◽  
Vol 32 ◽  
pp. 138-139
Author(s):  
V. F. Allison ◽  
G. C. Fink ◽  
G. W. Cearley
Keyword(s):  
Cell Growth ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Seminal Vesicle ◽  
Seminal Vesicles ◽  
Epithelial Layer ◽  
Epithelial Hyperplasia ◽  
Electron Microscopic ◽  
Aging Rats ◽  
Pseudostratified Epithelium

It is well known that epithelial hyperplasia (benign hypertrophy) is common in the aging prostate of dogs and man. In contrast, little evidence is available for abnormal epithelial cell growth in seminal vesicles of aging animals. Recently, enlarged seminal vesicles were reported in senescent mice, however, that enlargement resulted from increased storage of secretion in the lumen and occurred concomitant to epithelial hypoplasia in that species.The present study is concerned with electron microscopic observations of changes occurring in the pseudostratified epithelium of the seminal vescles of aging rats. Special attention is given to certain non-epithelial cells which have entered the epithelial layer.

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