Aeromoniosis (Aeromonas salmonicida).

2020 ◽  
pp. 211-234
Author(s):  
Margaret Crumlish ◽  
Brian Austin
Keyword(s):  
Aeromonas Salmonicida

Infeksi Aeromonas salmonicida dari Berbagai Wilayah di Indonesia Pada Ikan Mas (Cyprinus carpio)

2011 ◽  
Vol 16 (2) ◽  
Author(s):  
Riza Priyatna ◽  
Soedarmanto Indarjulianto ◽  
Kurniasih Kurniasih
Keyword(s):  
Cyprinus Carpio ◽  
Aeromonas Salmonicida

Aeromonas salmonicida merupakan bakteri penyebab furunculosis pada ikan yang mengakibatkan kerugian ekonomi di dalam budidaya ikan air tawar. Penelitian bertujuan mengetahui gambaran darah ikan Mas (Cyprinus carpio) yang diinfeksi oleh A. salmonicida, juga dilakukan pemeriksaan histopatologi. Sebanyak empat isolat atipikal A. salmonicida telah diisolasi dari ikan di empat daerah di Indonesia yaitu Pontianak, Semarang, Yogyakarta, Jambi dan satu isolat atipikal A. salmonicida subjenis smithia dari ATCC sebagai kontrol. Sebanyak 45 ekor ikan mas berukuran 1215 cm dibagi menjadi lima kelompok. Kelompok 14 diinfeksi dengan A. salmonicida 0,1ml x 10 4 sel/ml secara intraperitoneal dari empat isolat berbeda. Kelompok ikan 5/kontrol tidak dilakukan infeksi bakteri. Isolat A. salmonicida yang berasal dari Pontianak menunjukkan jumlah leukosit total dan kadar hemoglobin meningkat jelas pada hari ke-7 sesudah infeksi, disertai peningkatan jumlah rata-rata heterofil, limfosit dan monosit pada hari ke-7 sesudah infeksi. Berdasarkan hasil pemeriksaan darah menunjukkan bahwa isolat A. salmonicida dari Pontianak merupakan isolat patogen yang menyebabkan reaksi akut jika dibandingkan dengan isolat dari daerah lain. Isolat A. salmonicida dari Yogyakarta menyebabkan perubahan patologi paling ringan. Isolat dari Jambi dan Pontianak menyebabkan lesi kulit hingga lapisan otot, epicarditis mulai hari ke-3 sesudah infeksi.

Changes in Immunological Parameters and Disease Resistance in Juvenile Coho Salmon (Oncorhynchus kisutch) in Response to Dehydroabietic Acid Exposure under Varying Thermal Conditions

2004 ◽  
Vol 39 (3) ◽  
pp. 175-182 ◽  
Author(s):  
Keith B. Tierney ◽  
Eric Stockner ◽  
Christopher J. Kennedy
Keyword(s):  
Coho Salmon ◽  
Thermal Conditions ◽  
Dehydroabietic Acid ◽  
Aeromonas Salmonicida ◽  
Acclimation Temperature ◽  
Dependent Manner ◽  
Immunological Parameters ◽  
Cold Temperatures ◽  
Temperature Shock ◽  
Exposure Temperature

Abstract This study explored the effects of a sublethal 96-h dehydroabietic acid (DHAA) exposure on aspects of the immune system of juvenile coho salmon under varying temperature conditions. Coho were exposed to DHAA concentrations below the determined LC50 value of 0.94 mg/L (95% confidence limits of 0.81 to 1.24 mg/L) for 96 h at either their acclimation temperature (8 or 18°C), or during an acute warm-shock (8 to 18°C) or cold-shock (18 to 8°C). Acclimation temperature alone significantly affected hematocrit (Hct), neutrophil respiratory burst activity (RBA) and leucocyte proportions. With temperature-shock, leucocrit (Lct), RBA and leucocyte proportions were altered. All parameters were affected by DHAA exposure, but not always in a dose-dependent manner. Across groups, DHAA caused Hct, lysozyme, thrombocyte, neutrophil and monocyte proportions to increase, and Lct, RBA and lymphocyte proportions to decrease. DHAA-temperature interactions resulted in the exacerbation of DHAA-induced effects. Exposure temperature had the most significant effect on the susceptibility of coho to Aeromonas salmonicida; fish were more susceptible at cold temperatures and when subjected to a temperature-shock compared to their respective controls. DHAA exposure modulated the response of temperature-shocked fish to this pathogen.

AsaGEI2d: a new variant of a genomic island identified in a group of Aeromonas salmonicida subsp. salmonicida isolated from France, which bears the pAsa7 plasmid

2021 ◽  
Author(s):  
Antony T Vincent ◽  
Laurent Intertaglia ◽  
Victor Loyer ◽  
Valérie E Paquet ◽  
Émilie Adouane ◽  
...  
Keyword(s):  
Genomic Island ◽  
Insertion Site ◽  
Aeromonas Salmonicida ◽  
Genomic Islands ◽  
The Other ◽  
Fish Pathogen ◽  
Integrase Gene ◽  
New Variant ◽  
Cat Gene ◽  
Unique Genes

Abstract Genomic islands (Aeromonas salmonicida genomic islands, AsaGEIs) are found worldwide in many isolates of Aeromonas salmonicida subsp. salmonicida, a fish pathogen. To date, five variants of AsaGEI (1a, 1b, 2a, 2b and 2c) have been described. Here, we investigate a sixth AsaGEI, which was identified in France between 2016 and 2019 in 20 A. salmonicida subsp. salmonicida isolates recovered from sick salmon all at the same location. This new AsaGEI shares the same insertion site in the chromosome as the other AsaGEI2s as they all have a homologous integrase gene. This new AsaGEI was thus named AsaGEI2d, and has 5 unique genes compared to the other AsaGEIs. The isolates carrying AsaGEI2d also bear the plasmid pAsa7, which was initially found in an isolate from Switzerland. This plasmid provides resistance to chloramphenicol thanks to a cat gene. This study reveals more about the diversity of the AsaGEIs.

Ultraviolet Treatment of Water for Destruction of Five Gram-Negative Bacteria Pathogenic to Fishes

1977 ◽  
Vol 34 (8) ◽  
pp. 1244-1249 ◽  
Author(s):  
G. L. Bullock ◽  
H. M. Stuckey
Keyword(s):  
Particulate Matter ◽  
Ultraviolet Irradiation ◽  
Vibrio Anguillarum ◽  
Spring Water ◽  
Aeromonas Salmonicida ◽  
Water Disinfection ◽  
Gram Negative Bacteria ◽  
Fish Pathogens ◽  
Gram Negative ◽  
Ultraviolet Treatment

Filtration (25 nm) and ultraviolet irradiation dosages of 13,100–29,400 microwatt seconds per square centimetre (μW∙s∙cm−2) effected a 99.98–100% reduction of five gram-negative fish pathogens — Aeromonas salmonicida, A. hydrophila, Vibrio anguillarum, Pseudomonas fluorescens, and the enteric redmouth organism in 12.5 °C clear spring water or spring water containing particulate matter. Filtration and a dosage of 4500 μW∙s∙cm−2 killed 99.83–100% of test strains in spring water and 4000–4750 μW∙s∙cm−2 killed 99.33–99.99% in water with particulate matter. Irradiation of unfiltered water containing particulate matter was less effective, especially at dosages of 5000 μW∙s∙cm−2 or less, which killed 97–99.94% of strains. Filtration and 13,100 μW∙s∙cm−2 irradiation of water containing A. salmonicida prevented transmission of furunculosis. Key words: ultraviolet irradiation, bacterial fish pathogens, water disinfection

Modulation of the mitogenic PHA response of carp, Cyprinus carpio L., by extracellular products of Aeromonas salmonicida

1987 ◽  
Vol 31 (sa) ◽  
pp. 133-143 ◽  
Author(s):  
C. N. Pourreau ◽  
M. B. H. Koopman ◽  
G. F. R. Hendriks ◽  
D. Evenberg ◽  
W. B. Muiswinkel
Keyword(s):  
Cyprinus Carpio ◽  
Aeromonas Salmonicida ◽  
Carp Cyprinus Carpio ◽  
Extracellular Products ◽  
Pha Response

scholarly journals Chimeric Protein IPath® with Chelating Activity Improves Atlantic Salmon’s Immunity against Infectious Diseases

Vaccines ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 361
Author(s):  
Valentina Valenzuela-Muñoz ◽  
Bárbara P. Benavente ◽  
Antonio Casuso ◽  
Yeny Leal ◽  
Cristian Gallardo-Escárate
Keyword(s):  
Immune Response ◽  
Atlantic Salmon ◽  
Iron Metabolism ◽  
Iron Transport ◽  
Aeromonas Salmonicida ◽  
Transcriptional Analysis ◽  
Control Fish ◽  
Control Group ◽  
Commercial Fish ◽  
Chelating Activity

Infection processes displayed by pathogens require the acquisition of essential inorganic nutrients and trace elements from the host to survive and proliferate. Without a doubt, iron is a crucial trace metal for all living organisms and also a pivotal component in the host–parasite interactions. In particular, the host reduces the iron available to face the infectious disease, increasing iron transport proteins’ expression and activating the heme synthesis and degradation pathways. Moreover, recent findings have suggested that iron metabolism modulation in fish promotes the immune response by reducing cellular iron toxicity. We hypothesized that recombinant proteins related to iron metabolism could modulate the fish’s immune system through iron metabolism and iron-responsive genes. Here a chimeric iron transport protein (IPath®) was bioinformatically designed and then expressed in a recombinant bacterial system. The IPath® protein showed a significant chelating activity under in vitro conditions and biological activity. Taking this evidence, a vaccine candidate based on IPath® was evaluated in Atlantic salmon challenged with three different fish pathogens. Experimental trials were conducted using two fish groups: one immunized with IPath® and another injected with adjutant as the control group. After 400 accumulated thermal units (ATUs), two different infection trials were performed. In the first one, fish were infected with the bacterium Aeromonas salmonicida, and in a second trial, fish were exposed to the ectoparasite Caligus rogercresseyi and subsequently infected with the intracellular bacterium Piscirickettsia salmonis. Fish immunized with IPath® showed a significant delay in the mortality curve in response to A. salmonicida and P. salmonis infections. However, no significant differences between infected and control fish groups were observed at the end of the experiment. Notably, sea lice burden reduction was observed in vaccinated Atlantic salmon. Transcriptional analysis evidenced a high modulation of iron-homeostasis-related genes in fish vaccinated with IPath® compared to the control group during the infection. Moreover, increasing expression of Atlantic salmon IgT was associated with IPath® immunization. This study provides evidence that the IPath® protein could be used as an antigen or booster in commercial fish vaccines, improving the immune response against relevant pathogens for salmon aquaculture.

scholarly journals Structure of the tetragonal surface virulence array protein and gene of Aeromonas salmonicida

1991 ◽  
Vol 266 (23) ◽  
pp. 15258-15265 ◽  
Author(s):  
S. Chu ◽  
S. Cavaignac ◽  
J. Feutrier ◽  
B.M. Phipps ◽  
M. Kostrzynska ◽  
...  
Keyword(s):  
Aeromonas Salmonicida

scholarly journals The color change and stress response of Atlantic salmon (Salmo salar L.) infected with Aeromonas salmonicida

2021 ◽  
Vol 20 ◽  
pp. 100664
Author(s):  
Mengmeng Yi ◽  
Huanda Lu ◽  
Yishuai Du ◽  
Guoxiang Sun ◽  
Ce Shi ◽  
...  
Keyword(s):  
Stress Response ◽  
Atlantic Salmon ◽  
Salmo Salar ◽  
Color Change ◽  
Aeromonas Salmonicida ◽  
Atlantic Salmon Salmo Salar
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