scholarly journals Changes in arterial blood pressure in hypertensive rats caused by long-term intake of milk fermented by Enterococcus faecalis CECT 5728

2005 ◽  
Vol 94 (1) ◽  
pp. 36-43 ◽  
Author(s):  
M. Miguel ◽  
B. Muguerza ◽  
E. Sánchez ◽  
M. A. Delgado ◽  
I. Recio ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Inhibitory Activity ◽  
Tap Water ◽  
Fermented Milk ◽  
Negative Control ◽  
Hypertensive Rats ◽  
Ace Inhibitory Activity ◽  
Arterial Blood ◽  
Ace Inhibitory

We have evaluated the changes in arterial blood pressure caused in spontaneously hypertensive rats (SHR) by long-term intake of an Enterococcus faecalis CECT 5728-fermented milk with significant angiotensin-converting enzyme (ACE)-inhibitory activity. After being weaned, male 3-week-old SHR were randomized into five groups. Until the 20th week of life, rats in each group were given one of the following drinking fluids: tap water (negative control 1), a fermented milk without ACE-inhibitory activity (negative control 2), captopril (100 mg/kg) (positive control), the E. faecalis CECT 5728-fermented milk that had significant ACE-inhibitory activity, or Ca-enriched E. faecalis CECT 5728-fermented milk. Animals in the different groups were then given tap water as drinking fluid from the 20th to 25th week of life. Systolic blood pressure (SBP) and diastolic blood pressure (DBP) were measured weekly in the rats, from the 6th to 25th week of life, by the tail-cuff method. A definite decrease in SBP and DBP could be observed in the rats treated with captopril and also in the rats that received the E. faecalis CECT 5728-fermented milks. The greatest antihypertensive effect was observed when the pharmacological treatment was administered. The effect of the Ca-enriched fermented milk was slightly more accentuated and more constant than the effect of the E. faecalis CECT 5728-fermented milk that had not been enriched in Ca. SBP and DBP increased in the treated SHR when the corresponding antihypertensive treatment was removed. Fermentation of milk with E. faecalis CECT 5728 may therefore be a successful strategy to produce a functional food with antihypertensive activity.

Effect of long-term intake of milk products on blood pressure in hypertensive rats

2002 ◽  
Vol 69 (1) ◽  
pp. 103-111 ◽  
Author(s):  
MARIKA SIPOLA ◽  
PIET FINCKENBERG ◽  
RIITTA KORPELA ◽  
HEIKKI VAPAATALO ◽  
MARJA-LEENA NURMINEN
Keyword(s):  
Blood Pressure ◽  
Blood Pressure Level ◽  
Skim Milk ◽  
Fermented Milk ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Milk Products ◽  
Ace Inhibitory ◽  
Fermented Milk Products

The effect of long-term intake of two fermented milk products on the development of hypertension was compared in young spontaneously hypertensive rats (SHR). The products contained tripeptides isoleucine-proline-proline (IPP) and valine-proline-proline (VPP), which have been shown to possess angiotensin converting enzyme (ACE) inhibitory activity. Six-week-old SHR were divided into four groups to receive orally ad libitum water, skim milk or two fermented milk products (fermented milk A or fermented milk B; the latter is commercially available in Japan with trade name Calpis®) for 14 weeks. The calculated intake of IPP was 0·4 mg/d and 0·2 mg/d in the groups receiving fermented milk A and B, respectively, whereas the corresponding amounts for VPP were 0·6 mg/d and 0·3 mg/d. Systolic blood pressure (SBP) was monitored weekly by tail-cuff method. The development of hypertension was significantly attenuated in both groups receiving fermented milk products, whereas skim milk did not affect blood pressure. The effect was detectable after 6 weeks of treatment. At the end of the experiment, the lowest blood pressure level was found in the group receiving fermented milk A: the SBP was 21 mm Hg lower than in the group receiving water and 10 mm Hg lower than in the group receiving fermented milk B. This difference could be explained by larger intake of ACE inhibitory tripeptides in the group receiving fermented milk A as compared with fermented milk B.

scholarly journals Hypotensive and Angiotensin-Converting Enzyme Inhibitory Activities ofEisenia fetidaExtract in Spontaneously Hypertensive Rats

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Shumei Mao ◽  
Chengde Li
Keyword(s):  
Blood Pressure ◽  
Inhibitory Activity ◽  
Spontaneously Hypertensive Rats ◽  
Ang Ii ◽  
Shr Rats ◽  
Hypertensive Rats ◽  
Ace Inhibitory Activity ◽  
Spontaneously Hypertensive ◽  
Ace Inhibitory

Objectives. This study aimed to investigate the antihypertensive effects of anEisenia fetidaextract (EFE) and its possible mechanisms in spontaneously hypertensive rats (SHR rats).Methods. Sixteen-week-old SHR rats and Wistar-Kyoto rats (WKY rats) were used in this study. Rats were, respectively, given EFE (EFE group), captopril (captopril group), or phosphate-buffered saline (PBS) (normal control group and SHR group) for 4 weeks. ACE inhibitory activity of EFEin vitrowas determined. The systolic blood pressure (SBP) and diastolic blood pressure (DBP) were measured using a Rat Tail-Cuff Blood Pressure System. Levels of angiotensin II (Ang II), aldosterone (Ald), and 6-keto-prostaglandin F1 alpha (6-keto-PGF1α) in plasma were determined by radioimmunoassay, and serum nitric oxide (NO) concentration was measured by Griess reagent systems.Results. EFE had marked ACE inhibitory activityin vitro(IC50= 2.5 mg/mL). After the 4-week drug management, SHR rats in EFE group and in captopril group had lower SBP and DBP, lower levels of Ang II and Ald, and higher levels of 6-keto-PGF1αand NO than the SHR rats in SHR group.Conclusion. These results indicate that EFE has hypotensive effects in SHR rats and its effects might be associated with its ACE inhibitory activity.

scholarly journals Comparative Study of Angiotensin I-Converting Enzyme (ACE) Inhibition of Soy Foods as Affected by Processing Methods and Protein Isolation

Processes ◽  
2020 ◽  
Vol 8 (8) ◽  
pp. 978 ◽  
Author(s):  
Cíntia L. Handa ◽  
Yan Zhang ◽  
Shweta Kumari ◽  
Jing Xu ◽  
Elza I. Ida ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Inhibitory Activity ◽  
Ace Inhibition ◽  
Converting Enzyme ◽  
Angiotensin I ◽  
Ace Inhibitory Activity ◽  
Soy Foods ◽  
Inhibitory Capacity ◽  
Inhibitory Potential ◽  
Ace Inhibitory

Angiotensin converting enzyme (ACE) converts angiotensin I into the vasoconstrictor angiotensin II and eventually elevates blood pressure. High blood pressure is a major risk factor for heart disease and stroke. Studies show peptides present anti-hypertensive activity by ACE inhibition. During food processing and digestion, food proteins may be hydrolyzed and release peptides. Our objective was to determine and compare the ACE inhibitory potential of fermented and non-fermented soy foods and isolated 7S and 11S protein fractions. Soy foods (e.g., soybean, natto, tempeh, yogurt, soymilk, tofu, soy-sprouts) and isolated proteins were in vitro digested prior to the determination of ACE inhibitory activity. Peptide molecular weight distribution in digested samples was analyzed and correlated with ACE inhibitory capacity. Raw and cooked soymilk showed the highest ACE inhibitory potential. Bacteria-fermented soy foods had higher ACE inhibitory activity than fungus-fermented soy food, and 3 day germinated sprouts had higher ACE inhibition than those germinated for 5 and 7 days. The 11S hydrolysates showed higher ACE inhibitory capacity than 7S. Peptides of 1–4.5 kDa showed a higher contribution to reducing IC50. This study provides evidence that soy foods and isolated 7S and 11S proteins may be used as functional foods or ingredients to prevent or control hypertension.

scholarly journals A Novel Angiotensin-I-Converting Enzyme (ACE) Inhibitory Peptide from Takifugu flavidus

Marine Drugs ◽  
2021 ◽  
Vol 19 (12) ◽  
pp. 651
Author(s):  
Yongchang Su ◽  
Shicheng Chen ◽  
Shuilin Cai ◽  
Shuji Liu ◽  
Nan Pan ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Inhibitory Activity ◽  
Gel Chromatography ◽  
Converting Enzyme ◽  
Angiotensin I ◽  
Ace Inhibitory Activity ◽  
Inhibitory Peptide ◽  
Ace Inhibitory Peptide ◽  
Angiotensin I Converting Enzyme ◽  
Ace Inhibitory

Alcalase, neutral protease, and pepsin were used to hydrolyze the skin of Takifugu flavidus. The T. flavidus hydrolysates (TFHs) with the maximum degree of hydrolysis (DH) and angiotensin-I-converting enzyme (ACE)-inhibitory activity were selected and then ultra-filtered to obtain fractions with components of different molecular weights (MWs) (<1, 1–3, 3–10, 10–50, and >50 kDa). The components with MWs < 1 kDa showed the strongest ACE-inhibitory activity with a half-maximal inhibitory concentration (IC50) of 0.58 mg/mL. Purification and identification using semi-preparative liquid chromatography, Sephadex G-15 gel chromatography, RP-HPLC, and LC–MS/MS yielded one new potential ACE-inhibitory peptide, PPLLFAAL (non-competitive suppression mode; IC50 of 28 μmmol·L−1). Molecular docking and molecular dynamics simulations indicated that the peptides should bind well to ACE and interact with amino acid residues and the zinc ion at the ACE active site. Furthermore, a short-term assay of antihypertensive activity in spontaneously hypertensive rats (SHRs) revealed that PPLLFAAL could significantly decrease the systolic blood pressure (SBP) and diastolic blood pressure (DBP) of SHRs after intravenous administration. These results suggested that PPLLFAAL may have potential applications in functional foods or pharmaceuticals as an antihypertensive agent.

scholarly journals Blood Pressure-Lowering Peptides from Neo-Fermented Buckwheat Sprouts: A New Approach to Estimating ACE-Inhibitory Activity

PLoS ONE ◽  
2014 ◽  
Vol 9 (9) ◽  
pp. e105802 ◽  
Author(s):  
Masahiro Koyama ◽  
Seiji Hattori ◽  
Yoshihiko Amano ◽  
Masanori Watanabe ◽  
Kozo Nakamura
Keyword(s):  
Blood Pressure ◽  
Inhibitory Activity ◽  
Blood Pressure Lowering ◽  
Ace Inhibitory Activity ◽  
New Approach ◽  
Pressure Lowering ◽  
Ace Inhibitory

scholarly journals Long-Term Effect of an AqueousFraxinus excelsiorL. Seed Extract in Spontaneously Hypertensive Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Noemi López-Carreras ◽  
Sandra Fernández-Vallinas ◽  
Marta Miguel ◽  
Amaya Aleixandre
Keyword(s):  
Blood Pressure ◽  
Body Weight ◽  
Spontaneously Hypertensive Rats ◽  
Seed Extract ◽  
The Body ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Arterial Blood ◽  
Plasma Antioxidant Capacity

The effect of long-term intake of different doses (20, 40, and 60 mg/kg/day) of aFraxinus excelsiorL. seed extract (FESE) on spontaneously hypertensive rats (SHR) was evaluated. Water was used as control and captopril (50 mg/kg/day) was used as positive control. Systolic blood pressure, body weight, and food and liquid intake were registered weekly in SHR. The antioxidant and vascular relaxing properties of FESE were also studied in these animals. The development of hypertension was attenuated in the groups treated with captopril or FESE. The antihypertensive effect was more accentuated in the captopril group than in the FESE groups, and it was paradoxically more accentuated in the groups treated with 20 mg/kg/day or 40 mg/kg/day of FESE than in the group treated with the highest dose of this extract. Body weight gain and food intake increased in the FESE groups. After removing the corresponding antihypertensive treatment, the arterial blood pressure and the body weight of the FESE treated animals returned to control values. In addition, FESE increased plasma antioxidant capacity and decreased plasma and liver malondialdehyde levels. Moreover, acetylcholine relaxation improved in the aorta rings from the FESE treated rats.

Short-term effect of egg-white hydrolysate products on the arterial blood pressure of hypertensive rats

2005 ◽  
Vol 94 (5) ◽  
pp. 731-737 ◽  
Author(s):  
Marta Miguel ◽  
Rosina López-Fandiño ◽  
Mercedes Ramos ◽  
Amaya Aleixandre
Keyword(s):  
Blood Pressure ◽  
Egg White ◽  
Therapeutic Benefit ◽  
Negative Control ◽  
Hypertensive Rats ◽  
Blood Pressure Lowering Effect ◽  
Wky Rats ◽  
Arterial Blood ◽  
Wistar Kyoto

In the present study we evaluate the blood pressure-lowering effect of the following products: the hydrolysate obtained from egg white (EW) by enzymatic treatment with pepsin (HEW), the peptide fraction of HEW with molecular mass lower than 3000 Da (HEW<3000 Da), and three peptide sequences isolated from HEW<3000 Da (Tyr-Ala-Glu-Glu-Arg-Tyr-Pro-Ile-Leu: YAEERYPIL); (Arg-Ala-Asp-His-Pro-Phe-Leu: RADHPFL); and (Ile-Val-Phe (IVF)). These peptides, and also HEW and HEW<3000 Da, had been characterized previously in vitro as potent inhibitors of angiotensin-converting enzyme (ACE). EW and the products mentioned earlier were orally administered by gastric intubation, to 17–20-week-old male spontaneously hypertensive rats (SHR) and normotensive Wistar–Kyoto (WKY) rats. We measured the systolic blood pressure (SBP) and the diastolic blood pressure (DBP) of the rats by the tail cuff method before administration and also 2, 4, 6, 8 and 24h post-administration. Distilled water served as negative control, and we used captopril (50mg/kg) as positive control to carry out similar experiments with a known ACE inhibitor. HEW, HEW<3000 Da and the three peptide sequences decreased SBP and DBP in SHR but they did not modify these variables in WKY rats. The peptide sequences YAEERYPIL, RADHPFL and IVF showed a potency to decrease blood pressure greater than HEW or HEW<3000 Da. The results obtained suggest that the studied products could be used as a functional food with potential therapeutic benefit in the prevention and treatment of hypertension.

scholarly journals Lisinopril Indifferently Improves Heart Rate Variability During Day and Night Periods in Spontaneously Hypertensive Rats

2013 ◽  
pp. 237-245 ◽  
Author(s):  
S. ALBARWANI ◽  
S. AL-SIYABI ◽  
M. O. TANIRA
Keyword(s):  
Blood Pressure ◽  
Heart Rate ◽  
Heart Rate Variability ◽  
Spontaneously Hypertensive Rats ◽  
Radio Telemetry ◽  
Tap Water ◽  
Fold Increase ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Arterial Blood

The aim of this work was to investigate the effect of 10 weeks of lisinopril treatment to spontaneously hypertensive rats (SHRs) on day/night variations of blood pressure, heart rate and autonomic cardio-regulation parameters. Male SHR with surgically implanted radio-telemetry implant that provided direct measurements of arterial pressure and electrocardiogram wave were used. Animals were allocated to two groups (n=5 each). The first group was treated with lisinopril (20 mg/kg by gavage) daily for 10 weeks (treated group); whereas the second was gavaged daily with tap water (untreated group). Arterial blood pressure, ECG and other telemetry parameters were recorded at the start and at the end of 10-week treatment. Collected data were analyzed using specialized software and were statistically tested. In addition to the expected lowering of blood pressure, spectral analysis of R-R intervals revealed that lisinopril treatment for 10 weeks significantly caused 2-3 fold increase in heart rate variability (HRV) during both active and inactive periods. However, R-R interval durations demonstrated variable distribution patterns during those periods. The cause of observed distribution pattern of R-R intervals during active and inactive periods may be of significance to better understand HRV changes and warrants further investigations.

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